Diagnostic accuracy of blood centers in the screening of blood donors for viral markers.
Identifieur interne : 000250 ( PubMed/Checkpoint ); précédent : 000249; suivant : 000251Diagnostic accuracy of blood centers in the screening of blood donors for viral markers.
Auteurs : Elliot Eli Dogbe [Ghana] ; Fareed Arthur [Ghana]Source :
- The Pan African medical journal [ 1937-8688 ] ; 2015.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (sang), Antigènes de surface du virus de l'hépatite B (sang), Antigènes viraux (sang), Banques de sang, Donneurs de sang, Dosage radioimmunologique, Dépistage systématique (normes), Faux négatifs, Faux positifs, Ghana (épidémiologie), Hepacivirus (immunologie), Humains, Hépatite B (), Hépatite B (diagnostic), Hépatite B (sang), Hépatite B (épidémiologie), Hépatite C (), Hépatite C (diagnostic), Hépatite C (sang), Hépatite C (épidémiologie), Immunochromatographie, Infections à VIH (), Infections à VIH (diagnostic), Infections à VIH (sang), Infections à VIH (épidémiologie), Marqueurs biologiques (sang), Sensibilité et spécificité, Test ELISA, Transfusion sanguine, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (immunologie), Valeur prédictive des tests, Virus de l'hépatite B (immunologie), Virémie (), Virémie (diagnostic), Virémie (sang), Virémie (transmission), Virémie (épidémiologie), Évaluation de la compétence des laboratoires.
- MESH :
- diagnostic : Hépatite B, Hépatite C, Infections à VIH, Virémie.
- immunologie : Hepacivirus, VIH-1 (Virus de l'Immunodéficience Humaine de type 1), Virus de l'hépatite B.
- normes : Dépistage systématique.
- sang : Anticorps antiviraux, Antigènes de surface du virus de l'hépatite B, Antigènes viraux, Hépatite B, Hépatite C, Infections à VIH, Marqueurs biologiques, Virémie.
- épidémiologie : Ghana, Hépatite B, Hépatite C, Infections à VIH, Virémie.
- Banques de sang, Donneurs de sang, Dosage radioimmunologique, Faux négatifs, Faux positifs, Humains, Hépatite B, Hépatite C, Immunochromatographie, Infections à VIH, Sensibilité et spécificité, Test ELISA, Transfusion sanguine, Valeur prédictive des tests, Virémie, Évaluation de la compétence des laboratoires.
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Antigens, Viral (blood), Biomarkers (blood), Blood Banks, Blood Donors, Blood Transfusion, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, Ghana (epidemiology), HIV Infections (blood), HIV Infections (diagnosis), HIV Infections (epidemiology), HIV Infections (prevention & control), HIV-1 (immunology), Hepacivirus (immunology), Hepatitis B (blood), Hepatitis B (diagnosis), Hepatitis B (epidemiology), Hepatitis B (prevention & control), Hepatitis B Surface Antigens (blood), Hepatitis B virus (immunology), Hepatitis C (blood), Hepatitis C (diagnosis), Hepatitis C (epidemiology), Hepatitis C (prevention & control), Humans, Immunochromatography, Laboratory Proficiency Testing, Mass Screening (standards), Predictive Value of Tests, Radioimmunoassay, Sensitivity and Specificity, Viremia (blood), Viremia (diagnosis), Viremia (epidemiology), Viremia (prevention & control), Viremia (transmission).
- MESH :
- chemical , blood : Antibodies, Viral, Antigens, Viral, Biomarkers, Hepatitis B Surface Antigens.
- geographic , epidemiology : Ghana.
- blood : HIV Infections, Hepatitis B, Hepatitis C, Viremia.
- diagnosis : HIV Infections, Hepatitis B, Hepatitis C, Viremia.
- epidemiology : HIV Infections, Hepatitis B, Hepatitis C, Viremia.
- immunology : HIV-1, Hepacivirus, Hepatitis B virus.
- prevention & control : HIV Infections, Hepatitis B, Hepatitis C, Viremia.
- standards : Mass Screening.
- transmission : Viremia.
- Blood Banks, Blood Donors, Blood Transfusion, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, Humans, Immunochromatography, Laboratory Proficiency Testing, Predictive Value of Tests, Radioimmunoassay, Sensitivity and Specificity.
Abstract
Blood transfusion still remains a life saving intervention in almost all healthcare facilities worldwide. Screening of blood donors/blood units is done in almost every blood bank facility before the blood units/blood components are transfused to prevent transfusion-transmissible infections. The kind of testing kits or the methods used by a facility and the technical expertise of the personnel greatly affects the screening results of a facility. This study was aimed at evaluating the diagnostic accuracy of five hospital-based blood bank testing facilities (Komfo Anokye Teaching Hospital KNUST, Kwame Nkrumah University of Science and Technology, Agogo, Bekwai and Sunyani) that used rapid immunochromatograhic assays (RIA) in screening blood donors/blood units in Ghana.
DOI: 10.11604/pamj.2015.20.119.5263
PubMed: 26090067
Affiliations:
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(immunologie)</term><term>Valeur prédictive des tests</term><term>Virus de l'hépatite B (immunologie)</term><term>Virémie ()</term><term>Virémie (diagnostic)</term><term>Virémie (sang)</term><term>Virémie (transmission)</term><term>Virémie (épidémiologie)</term><term>Évaluation de la compétence des laboratoires</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Biomarkers</term><term>Hepatitis B Surface Antigens</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>HIV Infections</term><term>Hepatitis B</term><term>Hepatitis C</term><term>Viremia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>HIV Infections</term><term>Hepatitis B</term><term>Hepatitis C</term><term>Viremia</term></keywords><keywords scheme="MESH" 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Testing</term><term>Predictive Value of Tests</term><term>Radioimmunoassay</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banques de sang</term><term>Donneurs de sang</term><term>Dosage radioimmunologique</term><term>Faux négatifs</term><term>Faux positifs</term><term>Humains</term><term>Hépatite B</term><term>Hépatite C</term><term>Immunochromatographie</term><term>Infections à VIH</term><term>Sensibilité et spécificité</term><term>Test ELISA</term><term>Transfusion sanguine</term><term>Valeur prédictive des tests</term><term>Virémie</term><term>Évaluation de la compétence des laboratoires</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Blood transfusion still remains a life saving intervention in almost all healthcare facilities worldwide. Screening of blood donors/blood units is done in almost every blood bank facility before the blood units/blood components are transfused to prevent transfusion-transmissible infections. The kind of testing kits or the methods used by a facility and the technical expertise of the personnel greatly affects the screening results of a facility. This study was aimed at evaluating the diagnostic accuracy of five hospital-based blood bank testing facilities (Komfo Anokye Teaching Hospital KNUST, Kwame Nkrumah University of Science and Technology, Agogo, Bekwai and Sunyani) that used rapid immunochromatograhic assays (RIA) in screening blood donors/blood units in Ghana.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26090067</PMID><DateCreated><Year>2015</Year><Month>06</Month><Day>19</Day></DateCreated><DateCompleted><Year>2016</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1937-8688</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>The Pan African medical journal</Title><ISOAbbreviation>Pan Afr Med J</ISOAbbreviation></Journal><ArticleTitle>Diagnostic accuracy of blood centers in the screening of blood donors for viral markers.</ArticleTitle><Pagination><MedlinePgn>119</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.11604/pamj.2015.20.119.5263</ELocationID><Abstract><AbstractText Label="INTRODUCTION" NlmCategory="BACKGROUND">Blood transfusion still remains a life saving intervention in almost all healthcare facilities worldwide. Screening of blood donors/blood units is done in almost every blood bank facility before the blood units/blood components are transfused to prevent transfusion-transmissible infections. The kind of testing kits or the methods used by a facility and the technical expertise of the personnel greatly affects the screening results of a facility. This study was aimed at evaluating the diagnostic accuracy of five hospital-based blood bank testing facilities (Komfo Anokye Teaching Hospital KNUST, Kwame Nkrumah University of Science and Technology, Agogo, Bekwai and Sunyani) that used rapid immunochromatograhic assays (RIA) in screening blood donors/blood units in Ghana.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Blood samples (300) from the five testing facilities and their screening results for hepatitis B surface antigen (HBsAg), antibodies to hepatitis C virus (HCV) and human immunodeficiency virus (HIV) using RIAs were obtained. All the samples were then analysed for the three viral markers using 3(rd) generational enzyme linked immunosorbent assay (ELISA) kit as the gold standard.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The mean false positive for HBsAg was 2.2% with Bekwai testing facility having the highest of 4.4%. For HCV, the mean false positive was 2.8% with Agogo and Bekwai testing facilities having the highest of 8.7% respectively. For HIV screening, the mean false positive was 11.1% with Bekwai testing facility having the highest of 28.0%. The mean false negative for the facilities were 3.0% for HBV, 75.0% for HCV and 0.0% for HIV with KATH having the highest of 6.3% for HBV, Bekwai having the highest of 100% for HCV and no facility showing false negative for HIV. Mean sensitivity of the screening procedure for the facilities was 97.0%, 25.0% and 100.0% whilst the mean specificity was 97.8%, 97.2% and 88.9% for HBV, HCV and HIV respectively. Statistical comparison among the testing facilities showed no significant differences among the various testing centres for HBV screening; however, significant differences were obtained for HCV and HIV screening.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This study has shown that there is no standardised screening procedure for blood bank testing facilities in the country. There is therefore an urgent need for an internal and external control body to oversee screening procedures in blood banks across the country.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dogbe</LastName><ForeName>Elliot Eli</ForeName><Initials>EE</Initials><AffiliationInfo><Affiliation>Transfusion Medicine Unit, Komfo Anokye Teaching Hospital, Kumasi, Ghana.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arthur</LastName><ForeName>Fareed</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>02</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Uganda</Country><MedlineTA>Pan Afr Med J</MedlineTA><NlmUniqueID>101517926</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000956">Antigens, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006514">Hepatitis B Surface Antigens</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Transfusion. 2000 Oct;40(10):1157-60</RefSource><PMID 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PubStatus="accepted"><Year>2014</Year><Month>11</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>6</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>6</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26090067</ArticleId><ArticleId IdType="doi">10.11604/pamj.2015.20.119.5263</ArticleId><ArticleId IdType="pii">PAMJ-20-119</ArticleId><ArticleId IdType="pmc">PMC4458317</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Ghana</li></country></list><tree><country name="Ghana"><noRegion><name sortKey="Dogbe, Elliot Eli" sort="Dogbe, Elliot Eli" uniqKey="Dogbe E" first="Elliot Eli" last="Dogbe">Elliot 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