SidaGhanaV1, Pmc, Corpus, bibRecord, 000342

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Identifieur interne : 000342 ( Pmc/Corpus ); précédent : 000341; suivant : 000343

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Auteurs : Augustina A. Sylverken ; Ellis Owusu-Dabo ; Denis D. Yar ; Samson P. Salifu ; Nana Yaa Awua-Boateng ; John H. Amuasi ; Portia B. Okyere ; Thomas Agyarko-Poku

Source :

Ghana Medical Journal [ 0016-9560 ] ; 2016.

RBID : PMC:5044789

Abstract

SummaryBackground

Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed.

Methods

We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana.

Results

Although a total 78.00% of the women were asymptomatic, 77.1% of them tested positive for at least one bacterial STI pathogen. Mycoplasma genitalium was the most commonly detectable pathogen present in 67.5% of all women. Of those testing positive, 25.0% had single infections, while 38.0% and 19.5% had double and triple infections respectively. Altogether, 86.54% and 90.91% of the symptomatic and asymptomatic women respectively tested positive for at least one pathogen (p<0.05). There were no significant associations (p<0.05) between the clinical manifestations of the symptomatic women and the pathogens detected in their samples.

Conclusions

Our study confirmed the importance of complementing the syndromic approach to STI management with pathogen detection and most importantly recognise that STIs in women are asymptomatic and regular empirical testing even for both symptomatic and asymptomatic patients is critical for complete clinical treatment.

Funding

EOD (Ellis Owusu-Dabo Research working group, KCCR)

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5044789

PubMed: 27752188
PubMed Central: 5044789

Links to Exploration step

PMC:5044789

Le document en format XML

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<author><name sortKey="Sylverken, Augustina A" sort="Sylverken, Augustina A" uniqKey="Sylverken A" first="Augustina A" last="Sylverken">Augustina A. Sylverken</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Owusu Dabo, Ellis" sort="Owusu Dabo, Ellis" uniqKey="Owusu Dabo E" first="Ellis" last="Owusu-Dabo">Ellis Owusu-Dabo</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">School of Public Health, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yar, Denis D" sort="Yar, Denis D" uniqKey="Yar D" first="Denis D" last="Yar">Denis D. Yar</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Salifu, Samson P" sort="Salifu, Samson P" uniqKey="Salifu S" first="Samson P" last="Salifu">Samson P. Salifu</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A4">Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Awua Boateng, Nana Yaa" sort="Awua Boateng, Nana Yaa" uniqKey="Awua Boateng N" first="Nana Yaa" last="Awua-Boateng">Nana Yaa Awua-Boateng</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
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<author><name sortKey="Amuasi, John H" sort="Amuasi, John H" uniqKey="Amuasi J" first="John H" last="Amuasi">John H. Amuasi</name>
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</affiliation>
</author>
<author><name sortKey="Okyere, Portia B" sort="Okyere, Portia B" uniqKey="Okyere P" first="Portia B" last="Okyere">Portia B. Okyere</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Agyarko Poku, Thomas" sort="Agyarko Poku, Thomas" uniqKey="Agyarko Poku T" first="Thomas" last="Agyarko-Poku">Thomas Agyarko-Poku</name>
<affiliation><nlm:aff id="A5">Suntreso Government Hospital, Ghana Health Services, Kumasi, Ghana</nlm:aff>
</affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR</title>
<author><name sortKey="Sylverken, Augustina A" sort="Sylverken, Augustina A" uniqKey="Sylverken A" first="Augustina A" last="Sylverken">Augustina A. Sylverken</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A2">Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
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<author><name sortKey="Owusu Dabo, Ellis" sort="Owusu Dabo, Ellis" uniqKey="Owusu Dabo E" first="Ellis" last="Owusu-Dabo">Ellis Owusu-Dabo</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A3">School of Public Health, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yar, Denis D" sort="Yar, Denis D" uniqKey="Yar D" first="Denis D" last="Yar">Denis D. Yar</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Salifu, Samson P" sort="Salifu, Samson P" uniqKey="Salifu S" first="Samson P" last="Salifu">Samson P. Salifu</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="A4">Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Awua Boateng, Nana Yaa" sort="Awua Boateng, Nana Yaa" uniqKey="Awua Boateng N" first="Nana Yaa" last="Awua-Boateng">Nana Yaa Awua-Boateng</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Amuasi, John H" sort="Amuasi, John H" uniqKey="Amuasi J" first="John H" last="Amuasi">John H. Amuasi</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Okyere, Portia B" sort="Okyere, Portia B" uniqKey="Okyere P" first="Portia B" last="Okyere">Portia B. Okyere</name>
<affiliation><nlm:aff id="A1">Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Agyarko Poku, Thomas" sort="Agyarko Poku, Thomas" uniqKey="Agyarko Poku T" first="Thomas" last="Agyarko-Poku">Thomas Agyarko-Poku</name>
<affiliation><nlm:aff id="A5">Suntreso Government Hospital, Ghana Health Services, Kumasi, Ghana</nlm:aff>
</affiliation>
</author>
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<series><title level="j">Ghana Medical Journal</title>
<idno type="ISSN">0016-9560</idno>
<imprint><date when="2016">2016</date>
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<front><div type="abstract" xml:lang="en"><title>Summary</title>
<sec><title>Background</title>
<p>Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana.</p>
</sec>
<sec sec-type="results"><title>Results</title>
<p>Although a total 78.00% of the women were asymptomatic, 77.1% of them tested positive for at least one bacterial STI pathogen. <italic>Mycoplasma genitalium</italic>
 was the most commonly detectable pathogen present in 67.5% of all women. Of those testing positive, 25.0% had single infections, while 38.0% and 19.5% had double and triple infections respectively. Altogether, 86.54% and 90.91% of the symptomatic and asymptomatic women respectively tested positive for at least one pathogen (p<0.05). There were no significant associations (p<0.05) between the clinical manifestations of the symptomatic women and the pathogens detected in their samples.</p>
</sec>
<sec sec-type="conclusions"><title>Conclusions</title>
<p>Our study confirmed the importance of complementing the syndromic approach to STI management with pathogen detection and most importantly recognise that STIs in women are asymptomatic and regular empirical testing even for both symptomatic and asymptomatic patients is critical for complete clinical treatment.</p>
</sec>
<sec><title>Funding</title>
<p><bold>EOD (Ellis Owusu-Dabo Research working group, KCCR)</bold>
</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Ghana Med J</journal-id>
<journal-title-group><journal-title>Ghana Medical Journal</journal-title>
</journal-title-group>
<issn pub-type="ppub">0016-9560</issn>
<publisher><publisher-name>Ghana Medical Association</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">27752188</article-id>
<article-id pub-id-type="pmc">5044789</article-id>
<article-id pub-id-type="publisher-id">jGMJ.v50.i3.pg142</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Sylverken</surname>
<given-names>Augustina A</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Owusu-Dabo</surname>
<given-names>Ellis</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Yar</surname>
<given-names>Denis D</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Salifu</surname>
<given-names>Samson P</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Awua-Boateng</surname>
<given-names>Nana Yaa</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Amuasi</surname>
<given-names>John H</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Okyere</surname>
<given-names>Portia B</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Agyarko-Poku</surname>
<given-names>Thomas</given-names>
</name>
<xref ref-type="aff" rid="A5">5</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana</aff>
<aff id="A2">Department of Theoretical and Applied Biology, KNUST, Kumasi, Ghana</aff>
<aff id="A3"><label>3</label>
School of Public Health, KNUST, Kumasi, Ghana</aff>
<aff id="A4"><label>4</label>
Department of Biochemistry and Biotechnology, KNUST, Kumasi, Ghana</aff>
<aff id="A5"><label>5</label>
Suntreso Government Hospital, Ghana Health Services, Kumasi, Ghana</aff>
<author-notes><corresp>Corresponding author Dr. Ellis Owusu-Dabo <email>owusudabo@kccr.de</email>
</corresp>
<fn fn-type="COI-statement"><p><bold>Conflict of interest:</bold>
 None declared</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>9</month>
<year>2016</year>
</pub-date>
<volume>50</volume>
<issue>3</issue>
<fpage>142</fpage>
<lpage>148</lpage>
<permissions><copyright-statement>Copyright © Ghana Medical Association 2016</copyright-statement>
<copyright-year>2016</copyright-year>
</permissions>
<abstract abstract-type="executive-summary"><title>Summary</title>
<sec><title>Background</title>
<p>Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed.</p>
</sec>
<sec sec-type="methods"><title>Methods</title>
<p>We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana.</p>
</sec>
<sec sec-type="results"><title>Results</title>
<p>Although a total 78.00% of the women were asymptomatic, 77.1% of them tested positive for at least one bacterial STI pathogen. <italic>Mycoplasma genitalium</italic>
 was the most commonly detectable pathogen present in 67.5% of all women. Of those testing positive, 25.0% had single infections, while 38.0% and 19.5% had double and triple infections respectively. Altogether, 86.54% and 90.91% of the symptomatic and asymptomatic women respectively tested positive for at least one pathogen (p<0.05). There were no significant associations (p<0.05) between the clinical manifestations of the symptomatic women and the pathogens detected in their samples.</p>
</sec>
<sec sec-type="conclusions"><title>Conclusions</title>
<p>Our study confirmed the importance of complementing the syndromic approach to STI management with pathogen detection and most importantly recognise that STIs in women are asymptomatic and regular empirical testing even for both symptomatic and asymptomatic patients is critical for complete clinical treatment.</p>
</sec>
<sec><title>Funding</title>
<p><bold>EOD (Ellis Owusu-Dabo Research working group, KCCR)</bold>
</p>
</sec>
</abstract>
<kwd-group><kwd>Etiology</kwd>
<kwd>Syndromic</kwd>
<kwd>Sexually Transmitted Infections</kwd>
<kwd>Multiplex real time PCR</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>

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Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Bacterial etiology of sexually transmitted infections at a STI clinic in Ghana; use of multiplex real time PCR

Source :

Abstract

Links to Exploration step

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