Vertical transmission of HIV in ghanaian women diagnosed in cord blood and post-natal samples
Identifieur interne : 000096 ( PascalFrancis/Corpus ); précédent : 000095; suivant : 000097Vertical transmission of HIV in ghanaian women diagnosed in cord blood and post-natal samples
Auteurs : Lucia Fischetti ; Kwabena Danso ; Albert Dompreh ; Victor Addo ; Lars Haaheim ; Jean-Pierre AllainSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 103-107) or HIV-1 subtype and in utero transmission wasfound. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.
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Pour connaître la documentation sur le format Inist Standard.
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Format Inist (serveur)
NO : | PASCAL 06-0256799 INIST |
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ET : | Vertical transmission of HIV in ghanaian women diagnosed in cord blood and post-natal samples |
AU : | FISCHETTI (Lucia); DANSO (Kwabena); DOMPREH (Albert); ADDO (Victor); HAAHEIM (Lars); ALLAIN (Jean-Pierre) |
AF : | Department of Haematology, Division of Transfusion Medicine, University of Cambridge/Cambridge/Royaume-Uni (1 aut., 6 aut.); Department of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital/Kumasi/Ghana (2 aut., 4 aut.); Department of Microbiology, Komfo Anokye Teaching Hospital/Kumasi/Ghana (3 aut.); Department of Microbiology and Immunology, University of Bergen/Bergen/Norvège (5 aut.) |
DT : | Publication en série; Niveau analytique |
SO : | Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 3; Pp. 351-359; Bibl. 1 p.1/4 |
LA : | Anglais |
EA : | HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 103-107) or HIV-1 subtype and in utero transmission wasfound. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent. |
CC : | 002A05C10; 002B05C02J |
FD : | Virus immunodéficience humaine; Homme; Transmission verticale; Femelle; Sang; Ghana; Charge virale; Soustype |
FG : | Lentivirus; Retroviridae; Virus; Afrique |
ED : | Human immunodeficiency virus; Human; Vertical transmission; Female; Blood; Ghana; Viral load; Subtype |
EG : | Lentivirus; Retroviridae; Virus; Africa |
SD : | Human immunodeficiency virus; Hombre; Transmisión vertical; Hembra; Sangre; Ghana; Carga vírica; Subtipo |
LO : | INIST-17422.354000132096490040 |
ID : | 06-0256799 |
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Pascal:06-0256799Le document en format XML
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<front><div type="abstract" xml:lang="en">HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 10<sup>3</sup>
-10<sup>7</sup>
) or HIV-1 subtype and in utero transmission wasfound. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.</div>
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<server><NO>PASCAL 06-0256799 INIST</NO>
<ET>Vertical transmission of HIV in ghanaian women diagnosed in cord blood and post-natal samples</ET>
<AU>FISCHETTI (Lucia); DANSO (Kwabena); DOMPREH (Albert); ADDO (Victor); HAAHEIM (Lars); ALLAIN (Jean-Pierre)</AU>
<AF>Department of Haematology, Division of Transfusion Medicine, University of Cambridge/Cambridge/Royaume-Uni (1 aut., 6 aut.); Department of Obstetrics and Gynaecology, Komfo Anokye Teaching Hospital/Kumasi/Ghana (2 aut., 4 aut.); Department of Microbiology, Komfo Anokye Teaching Hospital/Kumasi/Ghana (3 aut.); Department of Microbiology and Immunology, University of Bergen/Bergen/Norvège (5 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of medical virology; ISSN 0146-6615; Coden JMVIDB; Etats-Unis; Da. 2005; Vol. 77; No. 3; Pp. 351-359; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 10<sup>3</sup>
-10<sup>7</sup>
) or HIV-1 subtype and in utero transmission wasfound. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.</EA>
<CC>002A05C10; 002B05C02J</CC>
<FD>Virus immunodéficience humaine; Homme; Transmission verticale; Femelle; Sang; Ghana; Charge virale; Soustype</FD>
<FG>Lentivirus; Retroviridae; Virus; Afrique</FG>
<ED>Human immunodeficiency virus; Human; Vertical transmission; Female; Blood; Ghana; Viral load; Subtype</ED>
<EG>Lentivirus; Retroviridae; Virus; Africa</EG>
<SD>Human immunodeficiency virus; Hombre; Transmisión vertical; Hembra; Sangre; Ghana; Carga vírica; Subtipo</SD>
<LO>INIST-17422.354000132096490040</LO>
<ID>06-0256799</ID>
</server>
</inist>
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