A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH
Identifieur interne : 000705 ( Ncbi/Merge ); précédent : 000704; suivant : 000706A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH
Auteurs : Alice Charwudzi [Ghana] ; Edeghonghon E. Olayemi [Ghana] ; Ivy Ekem [Ghana] ; Olufunmilayo Olopade [États-Unis] ; Mariann Coyle [États-Unis] ; Amma Anima Benneh [Ghana] ; Emmanuel Alote Allotey [Ghana]Source :
- Advances in Hematology [ 1687-9104 ] ; 2014.
Abstract
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DOI: 10.1155/2014/604165
PubMed: 25328527
PubMed Central: 4190273
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH</title><author><name sortKey="Charwudzi, Alice" sort="Charwudzi, Alice" uniqKey="Charwudzi A" first="Alice" last="Charwudzi">Alice Charwudzi</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast</wicri:regionArea><wicri:noRegion>Cape Coast</wicri:noRegion></affiliation></author><author><name sortKey="Olayemi, Edeghonghon E" sort="Olayemi, Edeghonghon E" uniqKey="Olayemi E" first="Edeghonghon E." last="Olayemi">Edeghonghon E. Olayemi</name><affiliation wicri:level="3"><nlm:aff id="I2">Department of Haematology, University of Ghana Medical School, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, University of Ghana Medical School, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Ekem, Ivy" sort="Ekem, Ivy" uniqKey="Ekem I" first="Ivy" last="Ekem">Ivy Ekem</name><affiliation wicri:level="3"><nlm:aff id="I2">Department of Haematology, University of Ghana Medical School, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, University of Ghana Medical School, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Olopade, Olufunmilayo" sort="Olopade, Olufunmilayo" uniqKey="Olopade O" first="Olufunmilayo" last="Olopade">Olufunmilayo Olopade</name><affiliation wicri:level="2"><nlm:aff id="I3">Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Coyle, Mariann" sort="Coyle, Mariann" uniqKey="Coyle M" first="Mariann" last="Coyle">Mariann Coyle</name><affiliation wicri:level="2"><nlm:aff id="I3">Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Benneh, Amma Anima" sort="Benneh, Amma Anima" uniqKey="Benneh A" first="Amma Anima" last="Benneh">Amma Anima Benneh</name><affiliation wicri:level="3"><nlm:aff id="I4">Department of Haematology, Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, Korle-Bu Teaching Hospital, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Allotey, Emmanuel Alote" sort="Allotey, Emmanuel Alote" uniqKey="Allotey E" first="Emmanuel Alote" last="Allotey">Emmanuel Alote Allotey</name><affiliation wicri:level="1"><nlm:aff id="I5">Haematology Unit, Tamale Teaching Hospital, Tamale, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Haematology Unit, Tamale Teaching Hospital, Tamale</wicri:regionArea><wicri:noRegion>Tamale</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25328527</idno><idno type="pmc">4190273</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190273</idno><idno type="RBID">PMC:4190273</idno><idno type="doi">10.1155/2014/604165</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000388</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000388</idno><idno type="wicri:Area/Pmc/Curation">000387</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000387</idno><idno type="wicri:Area/Pmc/Checkpoint">000269</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000269</idno><idno type="wicri:Area/Ncbi/Merge">000705</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH</title><author><name sortKey="Charwudzi, Alice" sort="Charwudzi, Alice" uniqKey="Charwudzi A" first="Alice" last="Charwudzi">Alice Charwudzi</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast</wicri:regionArea><wicri:noRegion>Cape Coast</wicri:noRegion></affiliation></author><author><name sortKey="Olayemi, Edeghonghon E" sort="Olayemi, Edeghonghon E" uniqKey="Olayemi E" first="Edeghonghon E." last="Olayemi">Edeghonghon E. Olayemi</name><affiliation wicri:level="3"><nlm:aff id="I2">Department of Haematology, University of Ghana Medical School, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, University of Ghana Medical School, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Ekem, Ivy" sort="Ekem, Ivy" uniqKey="Ekem I" first="Ivy" last="Ekem">Ivy Ekem</name><affiliation wicri:level="3"><nlm:aff id="I2">Department of Haematology, University of Ghana Medical School, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, University of Ghana Medical School, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Olopade, Olufunmilayo" sort="Olopade, Olufunmilayo" uniqKey="Olopade O" first="Olufunmilayo" last="Olopade">Olufunmilayo Olopade</name><affiliation wicri:level="2"><nlm:aff id="I3">Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Coyle, Mariann" sort="Coyle, Mariann" uniqKey="Coyle M" first="Mariann" last="Coyle">Mariann Coyle</name><affiliation wicri:level="2"><nlm:aff id="I3">Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637</wicri:regionArea><placeName><region type="state">Illinois</region></placeName></affiliation></author><author><name sortKey="Benneh, Amma Anima" sort="Benneh, Amma Anima" uniqKey="Benneh A" first="Amma Anima" last="Benneh">Amma Anima Benneh</name><affiliation wicri:level="3"><nlm:aff id="I4">Department of Haematology, Korle-Bu Teaching Hospital, Accra, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Department of Haematology, Korle-Bu Teaching Hospital, Accra</wicri:regionArea><placeName><settlement type="city">Accra</settlement><region nuts="2">Région du Grand Accra</region></placeName></affiliation></author><author><name sortKey="Allotey, Emmanuel Alote" sort="Allotey, Emmanuel Alote" uniqKey="Allotey E" first="Emmanuel Alote" last="Allotey">Emmanuel Alote Allotey</name><affiliation wicri:level="1"><nlm:aff id="I5">Haematology Unit, Tamale Teaching Hospital, Tamale, Ghana</nlm:aff><country xml:lang="fr">Ghana</country><wicri:regionArea>Haematology Unit, Tamale Teaching Hospital, Tamale</wicri:regionArea><wicri:noRegion>Tamale</wicri:noRegion></affiliation></author></analytic><series><title level="j">Advances in Hematology</title><idno type="ISSN">1687-9104</idno><idno type="eISSN">1687-9112</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Background. </italic>FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML.<italic> Methods.</italic> Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known<italic> BCR-ABL</italic> fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved.<italic> Result.</italic> 19 (95%) of the CML marrow smears demonstrated the<italic> BCR-ABL </italic>translocation. There was a significant correlation between the<italic> BCR-ABL</italic> transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (<italic>r</italic> = 0.870; <italic>P</italic> = 0.035).<italic> Conclusion.</italic> Archival methanol-fixed marrow and peripheral blood smears can be used to detect the<italic> BCR-ABL </italic>transcript for CML diagnosis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Le Gouill, S" uniqKey="Le Gouill S">S Le Gouill</name></author><author><name sortKey="Talmant, P" uniqKey="Talmant P">P Talmant</name></author><author><name sortKey="Milpied, N" uniqKey="Milpied N">N Milpied</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dewald, Gw" uniqKey="Dewald G">GW Dewald</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolff, Dj" uniqKey="Wolff D">DJ 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Adv Hematol</journal-id><journal-id journal-id-type="iso-abbrev">Adv Hematol</journal-id><journal-id journal-id-type="publisher-id">AH</journal-id><journal-title-group><journal-title>Advances in Hematology</journal-title></journal-title-group><issn pub-type="ppub">1687-9104</issn><issn pub-type="epub">1687-9112</issn><publisher><publisher-name>Hindawi Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25328527</article-id><article-id pub-id-type="pmc">4190273</article-id><article-id pub-id-type="doi">10.1155/2014/604165</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH</article-title></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0001-8932-3735</contrib-id><name><surname>Charwudzi</surname><given-names>Alice</given-names></name><xref ref-type="aff" rid="I1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Olayemi</surname><given-names>Edeghonghon E.</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-2426-314X</contrib-id><name><surname>Ekem</surname><given-names>Ivy</given-names></name><xref ref-type="aff" rid="I2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Olopade</surname><given-names>Olufunmilayo</given-names></name><xref ref-type="aff" rid="I3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid" authenticated="false">http://orcid.org/0000-0003-1878-7406</contrib-id><name><surname>Coyle</surname><given-names>Mariann</given-names></name><xref ref-type="aff" rid="I3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Benneh</surname><given-names>Amma Anima</given-names></name><xref ref-type="aff" rid="I4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Allotey</surname><given-names>Emmanuel Alote</given-names></name><xref ref-type="aff" rid="I5"><sup>5</sup></xref></contrib></contrib-group><aff id="I1"><sup>1</sup>Department of Chemical Pathology, University of Cape Coast School of Medical Sciences, Cape Coast, Ghana</aff><aff id="I2"><sup>2</sup>Department of Haematology, University of Ghana Medical School, Accra, Ghana</aff><aff id="I3"><sup>3</sup>Department of Medicine, University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA</aff><aff id="I4"><sup>4</sup>Department of Haematology, Korle-Bu Teaching Hospital, Accra, Ghana</aff><aff id="I5"><sup>5</sup>Haematology Unit, Tamale Teaching Hospital, Tamale, Ghana</aff><author-notes><corresp id="cor1">*Alice Charwudzi: <email>achawogi@yahoo.com</email></corresp><fn fn-type="other"><p>Academic Editor: Abdulkareem Almomen</p></fn></author-notes><pub-date pub-type="ppub"><year>2014</year></pub-date><pub-date pub-type="epub"><day>22</day><month>9</month><year>2014</year></pub-date><volume>2014</volume><elocation-id>604165</elocation-id><history><date date-type="received"><day>14</day><month>6</month><year>2014</year></date><date date-type="accepted"><day>7</day><month>9</month><year>2014</year></date></history><permissions><copyright-statement>Copyright © 2014 Alice Charwudzi et al.</copyright-statement><copyright-year>2014</copyright-year><license xlink:href="https://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p><italic>Background. </italic>FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML.<italic> Methods.</italic> Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had known<italic> BCR-ABL</italic> fusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved.<italic> Result.</italic> 19 (95%) of the CML marrow smears demonstrated the<italic> BCR-ABL </italic>translocation. There was a significant correlation between the<italic> BCR-ABL</italic> transcript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (<italic>r</italic> = 0.870; <italic>P</italic> = 0.035).<italic> Conclusion.</italic> Archival methanol-fixed marrow and peripheral blood smears can be used to detect the<italic> BCR-ABL </italic>transcript for CML diagnosis.</p></abstract></article-meta></front><floats-group><fig id="fig1" orientation="portrait" position="float"><label>Figure 1</label><caption><p><italic>BCR-ABL</italic> dual colour, dual fusion translocation probe hybridized to controls, and subject's bone marrow and peripheral blood smears. ((a1) and (b1)) Two red, two green (2R2G), negative control in a metaphase cell and an interphase cell, respectively; ((a2) and (b2)) five fusion, one green (5F1G), positive control in a metaphase and an interphase cell, respectively. (c)<italic> BCR-ABL</italic> negative FISH signal in a subject's peripheral blood smears. (d) 2F1R1G signals in a<italic> BCR-ABL</italic> positive subject BM smear. (e) 1F2R1G signals in a<italic> BCR-ABL</italic> positive subject marrow smear. (f) 2F1R in a<italic> BCR-ABL</italic> positive subject's peripheral blood (red cells lysed in glacial acetic acid: methanol fixative). Negative control is from a single<italic> BCR-ABL</italic> negative peripheral blood donor, and BV 173 is from a<italic> BCR-ABL</italic> positive cell line in blastic phase. Images were taken with ×100 oil immersion objective lens.</p></caption><graphic xlink:href="AH2014-604165.001"></graphic></fig><table-wrap id="tab1" orientation="portrait" position="float"><label>Table 1</label><caption><p>Pretreatment full blood count profile of study population.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" rowspan="2" colspan="1">Parameter </th><th align="center" rowspan="1" colspan="1">Study subject, <italic>N</italic> = 20</th><th align="center" rowspan="2" colspan="1"> Ranges </th></tr><tr><th align="center" rowspan="1" colspan="1">Median</th></tr></thead><tbody><tr><td align="left" rowspan="1" colspan="1">Leucocyte count (×10<sup>9</sup>/L)</td><td align="center" rowspan="1" colspan="1">253.1</td><td align="center" rowspan="1" colspan="1">20.7–636.8</td></tr><tr><td align="left" rowspan="1" colspan="1">Platelet count (×10<sup>9</sup>/L)</td><td align="center" rowspan="1" colspan="1">446.2<break></break></td><td align="center" rowspan="1" colspan="1">46.0–1051.0</td></tr><tr><td align="left" rowspan="1" colspan="1">Haemoglobin (g/dL)</td><td align="center" rowspan="1" colspan="1">8.2<break></break></td><td align="center" rowspan="1" colspan="1">5.2–11.5</td></tr><tr><td align="left" rowspan="1" colspan="1">Neutrophils (%)∗∗</td><td align="center" rowspan="1" colspan="1">67.6<break></break></td><td align="center" rowspan="1" colspan="1">45.7–89.3</td></tr><tr><td align="left" rowspan="1" colspan="1">Eosinophils (%)∗∗</td><td align="center" rowspan="1" colspan="1">2.2<break></break></td><td align="center" rowspan="1" colspan="1">0.1–12.3</td></tr><tr><td align="left" rowspan="1" colspan="1">Basophils (%)∗∗</td><td align="center" rowspan="1" colspan="1">4.5<break></break></td><td align="center" rowspan="1" colspan="1">0.0–25.0</td></tr></tbody></table><table-wrap-foot><fn><p><xref ref-type="table" rid="tab1">Table 1</xref> shows the full blood count profile of the 20 subjects at diagnosis. NB: % = percentage. ∗∗Absolute values for the differential counts were not available for majority of subjects at diagnosis; hence, percentage differential count was used.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list><country><li>Ghana</li><li>États-Unis</li></country><region><li>Illinois</li><li>Région du Grand Accra</li></region><settlement><li>Accra</li></settlement></list><tree><country name="Ghana"><noRegion><name sortKey="Charwudzi, Alice" sort="Charwudzi, Alice" uniqKey="Charwudzi A" first="Alice" last="Charwudzi">Alice Charwudzi</name></noRegion><name sortKey="Allotey, Emmanuel Alote" sort="Allotey, Emmanuel Alote" uniqKey="Allotey E" first="Emmanuel Alote" last="Allotey">Emmanuel Alote Allotey</name><name sortKey="Benneh, Amma Anima" sort="Benneh, Amma Anima" uniqKey="Benneh A" first="Amma Anima" last="Benneh">Amma Anima Benneh</name><name sortKey="Ekem, Ivy" sort="Ekem, Ivy" uniqKey="Ekem I" first="Ivy" last="Ekem">Ivy Ekem</name><name sortKey="Olayemi, Edeghonghon E" sort="Olayemi, Edeghonghon E" uniqKey="Olayemi E" first="Edeghonghon E." last="Olayemi">Edeghonghon E. Olayemi</name></country><country name="États-Unis"><region name="Illinois"><name sortKey="Olopade, Olufunmilayo" sort="Olopade, Olufunmilayo" uniqKey="Olopade O" first="Olufunmilayo" last="Olopade">Olufunmilayo Olopade</name></region><name sortKey="Coyle, Mariann" sort="Coyle, Mariann" uniqKey="Coyle M" first="Mariann" last="Coyle">Mariann Coyle</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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