Performance of HerpeSelect and Kalon Assays in Detection of Antibodies to Herpes Simplex Virus Type 2▿
Identifieur interne : 000276 ( Ncbi/Curation ); précédent : 000275; suivant : 000277Performance of HerpeSelect and Kalon Assays in Detection of Antibodies to Herpes Simplex Virus Type 2▿
Auteurs : Jérôme Legoff ; Philippe Mayaud ; Gérard Gresenguet ; Helen A. Weiss ; Khonde Nzambi ; Eric Frost ; Jacques Pepin ; Laurent BelecSource :
- Journal of Clinical Microbiology [ 0095-1137 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN viral (analyse), ADN viral (isolement et purification), Aciclovir (administration et posologie), Aciclovir (usage thérapeutique), Anticorps antiviraux (sang), Antiviraux (administration et posologie), Antiviraux (usage thérapeutique), Femelle, Ghana (épidémiologie), Herpès génital (diagnostic), Herpès génital (traitement médicamenteux), Herpès génital (virologie), Herpès génital (épidémiologie), Herpèsvirus humain de type 2 (génétique), Herpèsvirus humain de type 2 (immunologie), Herpèsvirus humain de type 2 (isolement et purification), Humains, Protéines de l'enveloppe virale (immunologie), Réaction de polymérisation en chaîne (), République centrafricaine (épidémiologie), Sensibilité et spécificité, Test ELISA (), Trousses de réactifs pour diagnostic.
- MESH :
- administration et posologie : Aciclovir, Antiviraux.
- analyse : ADN viral.
- diagnostic : Herpès génital.
- génétique : Herpèsvirus humain de type 2.
- immunologie : Herpèsvirus humain de type 2, Protéines de l'enveloppe virale.
- isolement et purification : ADN viral, Herpèsvirus humain de type 2.
- sang : Anticorps antiviraux.
- traitement médicamenteux : Herpès génital.
- usage thérapeutique : Aciclovir, Antiviraux.
- virologie : Herpès génital.
- épidémiologie : Ghana, Herpès génital, République centrafricaine.
- Femelle, Humains, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Test ELISA, Trousses de réactifs pour diagnostic.
- Wicri :
- geographic : République centrafricaine, Ghana.
English descriptors
- KwdEn :
- Acyclovir (administration & dosage), Acyclovir (therapeutic use), Antibodies, Viral (blood), Antiviral Agents (administration & dosage), Antiviral Agents (therapeutic use), Central African Republic (epidemiology), DNA, Viral (analysis), DNA, Viral (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Female, Ghana (epidemiology), Herpes Genitalis (diagnosis), Herpes Genitalis (drug therapy), Herpes Genitalis (epidemiology), Herpes Genitalis (virology), Herpesvirus 2, Human (genetics), Herpesvirus 2, Human (immunology), Herpesvirus 2, Human (isolation & purification), Humans, Polymerase Chain Reaction (methods), Reagent Kits, Diagnostic, Sensitivity and Specificity, Viral Envelope Proteins (immunology).
- MESH :
- chemical , administration & dosage : Acyclovir, Antiviral Agents.
- chemical , analysis : DNA, Viral.
- chemical , blood : Antibodies, Viral.
- chemical , immunology : Viral Envelope Proteins.
- chemical , isolation & purification : DNA, Viral.
- chemical , therapeutic use : Acyclovir, Antiviral Agents.
- geographic , epidemiology : Central African Republic, Ghana.
- diagnosis : Herpes Genitalis.
- drug therapy : Herpes Genitalis.
- epidemiology : Herpes Genitalis.
- genetics : Herpesvirus 2, Human.
- immunology : Herpesvirus 2, Human.
- isolation & purification : Herpesvirus 2, Human.
- methods : Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction.
- virology : Herpes Genitalis.
- Female, Humans, Reagent Kits, Diagnostic, Sensitivity and Specificity.
Abstract
The performances of commercial enzyme-linked immunosorbent assays (ELISAs) in detecting herpes simplex virus type 2 (HSV-2) antibodies have been inconsistent for African and human immunodeficiency virus (HIV)-positive populations. We compared the performances of the HerpeSelect and Kalon glycoprotein G2 ELISAs for patients with genital ulcer disease in Ghana and the Central African Republic. Sera from 434 women were tested with the HerpeSelect assay, and a subsample (
Url:
DOI: 10.1128/JCM.02332-07
PubMed: 18385443
PubMed Central: 2446869
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PMC:2446869Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyclovir (administration & dosage)</term>
<term>Acyclovir (therapeutic use)</term>
<term>Antibodies, Viral (blood)</term>
<term>Antiviral Agents (administration & dosage)</term>
<term>Antiviral Agents (therapeutic use)</term>
<term>Central African Republic (epidemiology)</term>
<term>DNA, Viral (analysis)</term>
<term>DNA, Viral (isolation & purification)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Female</term>
<term>Ghana (epidemiology)</term>
<term>Herpes Genitalis (diagnosis)</term>
<term>Herpes Genitalis (drug therapy)</term>
<term>Herpes Genitalis (epidemiology)</term>
<term>Herpes Genitalis (virology)</term>
<term>Herpesvirus 2, Human (genetics)</term>
<term>Herpesvirus 2, Human (immunology)</term>
<term>Herpesvirus 2, Human (isolation & purification)</term>
<term>Humans</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Reagent Kits, Diagnostic</term>
<term>Sensitivity and Specificity</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term>
<term>ADN viral (isolement et purification)</term>
<term>Aciclovir (administration et posologie)</term>
<term>Aciclovir (usage thérapeutique)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Antiviraux (administration et posologie)</term>
<term>Antiviraux (usage thérapeutique)</term>
<term>Femelle</term>
<term>Ghana (épidémiologie)</term>
<term>Herpès génital (diagnostic)</term>
<term>Herpès génital (traitement médicamenteux)</term>
<term>Herpès génital (virologie)</term>
<term>Herpès génital (épidémiologie)</term>
<term>Herpèsvirus humain de type 2 (génétique)</term>
<term>Herpèsvirus humain de type 2 (immunologie)</term>
<term>Herpèsvirus humain de type 2 (isolement et purification)</term>
<term>Humains</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>République centrafricaine (épidémiologie)</term>
<term>Sensibilité et spécificité</term>
<term>Test ELISA ()</term>
<term>Trousses de réactifs pour diagnostic</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Acyclovir</term>
<term>Antiviral Agents</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Acyclovir</term>
<term>Antiviral Agents</term>
</keywords>
<keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Central African Republic</term>
<term>Ghana</term>
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<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Aciclovir</term>
<term>Antiviraux</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term>
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<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Herpes Genitalis</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Herpesvirus 2, Human</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Herpèsvirus humain de type 2</term>
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<term>Protéines de l'enveloppe virale</term>
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<term>Herpèsvirus humain de type 2</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Polymerase Chain Reaction</term>
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<term>Sensitivity and Specificity</term>
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<front><div type="abstract" xml:lang="en"><p>The performances of commercial enzyme-linked immunosorbent assays (ELISAs) in detecting herpes simplex virus type 2 (HSV-2) antibodies have been inconsistent for African and human immunodeficiency virus (HIV)-positive populations. We compared the performances of the HerpeSelect and Kalon glycoprotein G2 ELISAs for patients with genital ulcer disease in Ghana and the Central African Republic. Sera from 434 women were tested with the HerpeSelect assay, and a subsample (<italic>n</italic>
= 199) was tested by the Kalon assay. Ulcer swabs and cervicovaginal lavage samples were tested for HSV-2 DNA by PCR. HSV-2-seronegative women with detectable genital HSV-2 DNA were retested for HSV-2 antibodies 14 and 28 days later by the two ELISAs. A total of 346 (80%) women were positive by HerpeSelect at baseline, and 225 (54%) had detectable genital (lesional or cervicovaginal) HSV-2 DNA. Sixty-six (19%) HerpeSelect-positive samples had low-positive index values (1.1 to 3.5), and 58% of these samples had detectable genital HSV-2 DNA. Global agreement between the two serological assays was 86%. Concordance was high (99%) for sera that were negative by HerpeSelect or had high index values (>3.5). Defining infection detected by HSV-2 DNA PCR and/or Kalon assay as true infection, 71% of sera with low-positive index values were associated with true HSV-2 infection. Twenty-five women were identified as having nonprimary first-episode genital HSV-2 infection. Rates of HSV-2 seroconversion at day 14 were 77% (10/13 patients) by HerpeSelect assay and 23% (3/13 patients) by Kalon assay, with four additional seroconversions detected by Kalon assay at day 28. HIV serostatus did not influence assay performance. Low index values obtained with the HerpeSelect assay may correspond to true HSV-2 infection, in particular to nonprimary first episodes of genital HSV-2 infection, and need to be interpreted in the context of clinical history.</p>
</div>
</front>
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