Vertical transmission of HIV in Ghanaian women diagnosed in cord blood and post-natal samples.
Identifieur interne : 000183 ( Ncbi/Curation ); précédent : 000182; suivant : 000184Vertical transmission of HIV in Ghanaian women diagnosed in cord blood and post-natal samples.
Auteurs : Lucia Fischetti [Royaume-Uni] ; Kwabena Danso ; Albert Dompreh ; Victor Addo ; Lars Haaheim ; Jean-Pierre AllainSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (sang), Analyse de séquence d'ADN, Charge virale, Complications de la grossesse et maladies infectieuses (diagnostic), Complications de la grossesse et maladies infectieuses (virologie), Données de séquences moléculaires, Femelle, Ghana (épidémiologie), Grossesse, Humains, Infections à VIH (diagnostic), Infections à VIH (transmission), Infections à VIH (virologie), Nouveau-né, Phylogénie, Prévalence, Sang foetal (virologie), Transmission verticale de maladie infectieuse, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (isolement et purification), VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (physiologie).
- MESH :
- diagnostic : Complications de la grossesse et maladies infectieuses, Infections à VIH.
- génétique : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- physiologie : VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- sang : ARN viral.
- virologie : Complications de la grossesse et maladies infectieuses, Infections à VIH, Sang foetal.
- épidémiologie : Ghana, Infections à VIH.
- Analyse de séquence d'ADN, Charge virale, Données de séquences moléculaires, Femelle, Grossesse, Humains, Nouveau-né, Phylogénie, Prévalence, Transmission verticale de maladie infectieuse.
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Female, Fetal Blood (virology), Ghana (epidemiology), HIV Infections (diagnosis), HIV Infections (transmission), HIV Infections (virology), HIV-1 (genetics), HIV-1 (isolation & purification), HIV-1 (physiology), Humans, Infant, Newborn, Infectious Disease Transmission, Vertical, Molecular Sequence Data, Phylogeny, Pregnancy, Pregnancy Complications, Infectious (diagnosis), Pregnancy Complications, Infectious (virology), Prevalence, RNA, Viral (blood), Sequence Analysis, DNA, Viral Load.
- MESH :
- chemical , blood : RNA, Viral.
- geographic , epidemiology : Ghana.
- diagnosis : HIV Infections, Pregnancy Complications, Infectious.
- genetics : HIV-1.
- isolation & purification : HIV-1.
- physiology : HIV-1.
- transmission : HIV Infections.
- virology : Fetal Blood, HIV Infections, Pregnancy Complications, Infectious.
- Female, Humans, Infant, Newborn, Infectious Disease Transmission, Vertical, Molecular Sequence Data, Phylogeny, Pregnancy, Prevalence, Sequence Analysis, DNA, Viral Load.
Abstract
HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 10(3)-10(7)) or HIV-1 subtype and in utero transmission was found. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.
DOI: 10.1002/jmv.20463
PubMed: 16173021
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pubmed:16173021Le document en format XML
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Most infections are thought to occur peri- or post-natally, hence preventive antiviral therapy administered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as determinants of transmission, and compared molecular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seropositive mother-cord blood paired plasmas from Ghana were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and analyzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 10(3)-10(7)) or HIV-1 subtype and in utero transmission was found. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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