The therapeutic landscape of HIV-1 via genome editing.
Identifieur interne : 000B65 ( Ncbi/Checkpoint ); précédent : 000B64; suivant : 000B66The therapeutic landscape of HIV-1 via genome editing.
Auteurs : Alexander Kwarteng [Ghana] ; Samuel Terkper Ahuno [Ghana] ; Godwin Kwakye-Nuako [Ghana]Source :
- AIDS research and therapy [ 1742-6405 ] ; 2017.
Abstract
Current treatment for HIV-1 largely relies on chemotherapy through the administration of antiretroviral drugs. While the search for anti-HIV-1 vaccine remain elusive, the use of highly active antiretroviral therapies (HAART) have been far-reaching and has changed HIV-1 into a manageable chronic infection. There is compelling evidence, including several side-effects of ARTs, suggesting that eradication of HIV-1 cannot depend solely on antiretrovirals. Gene therapy, an expanding treatment strategy, using RNA interference (RNAi) and programmable nucleases such as meganuclease, zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR-Cas9) are transforming the therapeutic landscape of HIV-1. TALENS and ZFNS are structurally similar modular systems, which consist of a FokI endonuclease fused to custom-designed effector proteins but have been largely limited, particularly ZFNs, due to their complexity and cost of protein engineering. However, the newly developed CRISPR-Cas9 system, consists of a single guide RNA (sgRNA), which directs a Cas9 endonuclease to complementary target sites, and serves as a superior alternative to the previous protein-based systems. The techniques have been successfully applied to the development of better HIV-1 models, generation of protective mutations in endogenous/host cells, disruption of HIV-1 genomes and even reactivating latent viruses for better detection and clearance by host immune response. Here, we focus on gene editing-based HIV-1 treatment and research in addition to providing perspectives for refining these techniques.
DOI: 10.1186/s12981-017-0157-8
PubMed: 28705213
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000571
- to stream PubMed, to step Curation: 000571
- to stream PubMed, to step Checkpoint: 000571
- to stream Ncbi, to step Merge: 000B65
- to stream Ncbi, to step Curation: 000B65
Links to Exploration step
pubmed:28705213Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The therapeutic landscape of HIV-1 via genome editing.</title>
<author><name sortKey="Kwarteng, Alexander" sort="Kwarteng, Alexander" uniqKey="Kwarteng A" first="Alexander" last="Kwarteng">Alexander Kwarteng</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi, Ghana. akwarteng@knust.edu.gh.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi</wicri:regionArea>
<wicri:noRegion>Kumasi</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ahuno, Samuel Terkper" sort="Ahuno, Samuel Terkper" uniqKey="Ahuno S" first="Samuel Terkper" last="Ahuno">Samuel Terkper Ahuno</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi, Ghana.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi</wicri:regionArea>
<wicri:noRegion>Kumasi</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kwakye Nuako, Godwin" sort="Kwakye Nuako, Godwin" uniqKey="Kwakye Nuako G" first="Godwin" last="Kwakye-Nuako">Godwin Kwakye-Nuako</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast</wicri:regionArea>
<wicri:noRegion>Cape Coast</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28705213</idno>
<idno type="pmid">28705213</idno>
<idno type="doi">10.1186/s12981-017-0157-8</idno>
<idno type="wicri:Area/PubMed/Corpus">000571</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000571</idno>
<idno type="wicri:Area/PubMed/Curation">000571</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000571</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000571</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000571</idno>
<idno type="wicri:Area/Ncbi/Merge">000B65</idno>
<idno type="wicri:Area/Ncbi/Curation">000B65</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000B65</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The therapeutic landscape of HIV-1 via genome editing.</title>
<author><name sortKey="Kwarteng, Alexander" sort="Kwarteng, Alexander" uniqKey="Kwarteng A" first="Alexander" last="Kwarteng">Alexander Kwarteng</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi, Ghana. akwarteng@knust.edu.gh.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi</wicri:regionArea>
<wicri:noRegion>Kumasi</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ahuno, Samuel Terkper" sort="Ahuno, Samuel Terkper" uniqKey="Ahuno S" first="Samuel Terkper" last="Ahuno">Samuel Terkper Ahuno</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi, Ghana.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology (KNUST), PMB, Kumasi</wicri:regionArea>
<wicri:noRegion>Kumasi</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kwakye Nuako, Godwin" sort="Kwakye Nuako, Godwin" uniqKey="Kwakye Nuako G" first="Godwin" last="Kwakye-Nuako">Godwin Kwakye-Nuako</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.</nlm:affiliation>
<country xml:lang="fr">Ghana</country>
<wicri:regionArea>Department of Biomedical Sciences, School of Allied Health Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast</wicri:regionArea>
<wicri:noRegion>Cape Coast</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">AIDS research and therapy</title>
<idno type="eISSN">1742-6405</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Current treatment for HIV-1 largely relies on chemotherapy through the administration of antiretroviral drugs. While the search for anti-HIV-1 vaccine remain elusive, the use of highly active antiretroviral therapies (HAART) have been far-reaching and has changed HIV-1 into a manageable chronic infection. There is compelling evidence, including several side-effects of ARTs, suggesting that eradication of HIV-1 cannot depend solely on antiretrovirals. Gene therapy, an expanding treatment strategy, using RNA interference (RNAi) and programmable nucleases such as meganuclease, zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR-Cas9) are transforming the therapeutic landscape of HIV-1. TALENS and ZFNS are structurally similar modular systems, which consist of a FokI endonuclease fused to custom-designed effector proteins but have been largely limited, particularly ZFNs, due to their complexity and cost of protein engineering. However, the newly developed CRISPR-Cas9 system, consists of a single guide RNA (sgRNA), which directs a Cas9 endonuclease to complementary target sites, and serves as a superior alternative to the previous protein-based systems. The techniques have been successfully applied to the development of better HIV-1 models, generation of protective mutations in endogenous/host cells, disruption of HIV-1 genomes and even reactivating latent viruses for better detection and clearance by host immune response. Here, we focus on gene editing-based HIV-1 treatment and research in addition to providing perspectives for refining these techniques.</div>
</front>
</TEI>
<affiliations><list><country><li>Ghana</li>
</country>
</list>
<tree><country name="Ghana"><noRegion><name sortKey="Kwarteng, Alexander" sort="Kwarteng, Alexander" uniqKey="Kwarteng A" first="Alexander" last="Kwarteng">Alexander Kwarteng</name>
</noRegion>
<name sortKey="Ahuno, Samuel Terkper" sort="Ahuno, Samuel Terkper" uniqKey="Ahuno S" first="Samuel Terkper" last="Ahuno">Samuel Terkper Ahuno</name>
<name sortKey="Kwakye Nuako, Godwin" sort="Kwakye Nuako, Godwin" uniqKey="Kwakye Nuako G" first="Godwin" last="Kwakye-Nuako">Godwin Kwakye-Nuako</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B65 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000B65 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= SidaGhanaV1 |flux= Ncbi |étape= Checkpoint |type= RBID |clé= pubmed:28705213 |texte= The therapeutic landscape of HIV-1 via genome editing. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:28705213" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a SidaGhanaV1
This area was generated with Dilib version V0.6.31. |