AIDS in an HIV-seronegative Ghanaian woman with intersubtype A/G recombinant HIV-1 infection
Identifieur interne : 001260 ( Main/Merge ); précédent : 001259; suivant : 001261AIDS in an HIV-seronegative Ghanaian woman with intersubtype A/G recombinant HIV-1 infection
Auteurs : D. Candotti [Royaume-Uni] ; Y. Adu-Sarkodie [Ghana] ; F. Davies [Royaume-Uni] ; E. Baldrich-Rubio [Royaume-Uni] ; K. Stirrups [Royaume-Uni] ; H. Lee [Royaume-Uni] ; J.-P. Allain [Royaume-Uni]Source :
- Journal of medical virology [ 0146-6615 ] ; 2000.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
Abstract
A 29-year-old Ghanaian woman who developed AIDS while being HIV-antibody seronegative was investigated during a collaborative study aimed at the identification of viral causes of a HIV-seronegative AIDS syndrome in West Africa. Plasma was screened with a panel of EIA tests for antibodies to HIV and HIV-1 p24 antigen. Retroviral infection was investigated by detection of reverse transcriptase (RT) activity in plasma, viral RNA amplification and quantification, and virus isolation. Positive amplification products were sequenced and phylogenetic analyses were carried out. Most EIA tests were unable to demonstrate the presence of anti-HIV antibodies, whereas confirmatory assays yielded inconclusive results. Retroviral infection was documented by detection of RT activity, HIV-1-specific genomic amplification and virus isolation. This virus was HIV-1 subtype A with an unusual six amino acid insertion in the gp120 V4 loop and with the nef gene of subtype G. The patient's plasma did not react with either autologous or heterologous viral lysates or HIV-1 peptides, whereas antibodies to other viral antigens were present. In conclusion, the Ghanaian patient exhibited a rare subtype A/G recombinant HIV-1 infection with a near absence of a HIV-specific humoral response. The lack of detectable antibody response might be due to either a highly pathogenic, rapidly fatal, HIV-1 infection preventing the development of the typical humoral immune response or to a host-related dysfunction of the immune system. Direct antigenemia or genomic detection of the virus should be undertaken when clinical or biological data suggests an HIV infection in the absence of serological evidence.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000143
- to stream PascalFrancis, to step Curation: 000006
- to stream PascalFrancis, to step Checkpoint: 000133
Links to Exploration step
Pascal:00-0429377Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">AIDS in an HIV-seronegative Ghanaian woman with intersubtype A/G recombinant HIV-1 infection</title><author><name sortKey="Candotti, D" sort="Candotti, D" uniqKey="Candotti D" first="D." last="Candotti">D. Candotti</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Adu Sarkodie, Y" sort="Adu Sarkodie, Y" uniqKey="Adu Sarkodie Y" first="Y." last="Adu-Sarkodie">Y. Adu-Sarkodie</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Komfo Anokye Teaching Hospital</s1><s2>Kumasi</s2><s3>GHA</s3><sZ>2 aut.</sZ></inist:fA14><country>Ghana</country><wicri:noRegion>Komfo Anokye Teaching Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Davies, F" sort="Davies, F" uniqKey="Davies F" first="F." last="Davies">F. Davies</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Baldrich Rubio, E" sort="Baldrich Rubio, E" uniqKey="Baldrich Rubio E" first="E." last="Baldrich-Rubio">E. Baldrich-Rubio</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Stirrups, K" sort="Stirrups, K" uniqKey="Stirrups K" first="K." last="Stirrups">K. Stirrups</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Lee, H" sort="Lee, H" uniqKey="Lee H" first="H." last="Lee">H. Lee</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Allain, J P" sort="Allain, J P" uniqKey="Allain J" first="J.-P." last="Allain">J.-P. Allain</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">00-0429377</idno><date when="2000">2000</date><idno type="stanalyst">PASCAL 00-0429377 INIST</idno><idno type="RBID">Pascal:00-0429377</idno><idno type="wicri:Area/PascalFrancis/Corpus">000143</idno><idno type="wicri:Area/PascalFrancis/Curation">000006</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000133</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000133</idno><idno type="wicri:doubleKey">0146-6615:2000:Candotti D:aids:in:an</idno><idno type="wicri:Area/Main/Merge">001260</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">AIDS in an HIV-seronegative Ghanaian woman with intersubtype A/G recombinant HIV-1 infection</title><author><name sortKey="Candotti, D" sort="Candotti, D" uniqKey="Candotti D" first="D." last="Candotti">D. Candotti</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Adu Sarkodie, Y" sort="Adu Sarkodie, Y" uniqKey="Adu Sarkodie Y" first="Y." last="Adu-Sarkodie">Y. Adu-Sarkodie</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Komfo Anokye Teaching Hospital</s1><s2>Kumasi</s2><s3>GHA</s3><sZ>2 aut.</sZ></inist:fA14><country>Ghana</country><wicri:noRegion>Komfo Anokye Teaching Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Davies, F" sort="Davies, F" uniqKey="Davies F" first="F." last="Davies">F. Davies</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Baldrich Rubio, E" sort="Baldrich Rubio, E" uniqKey="Baldrich Rubio E" first="E." last="Baldrich-Rubio">E. Baldrich-Rubio</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Stirrups, K" sort="Stirrups, K" uniqKey="Stirrups K" first="K." last="Stirrups">K. Stirrups</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Lee, H" sort="Lee, H" uniqKey="Lee H" first="H." last="Lee">H. Lee</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author><author><name sortKey="Allain, J P" sort="Allain, J P" uniqKey="Allain J" first="J.-P." last="Allain">J.-P. Allain</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Haematology, University of Cambridge</s1><s2>Cambridge</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName><orgName type="university">Université de Cambridge</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of medical virology</title><title level="j" type="abbreviated">J. med. virol.</title><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS</term><term>Antibody</term><term>Case study</term><term>Female</term><term>Ghana</term><term>HIV-1 virus</term><term>Human</term><term>Humoral immunity</term><term>Recombinant virus</term><term>Serology</term><term>Seronegativity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term><term>Homme</term><term>Femelle</term><term>Etude cas</term><term>Virus recombinant</term><term>Immunité humorale</term><term>Anticorps</term><term>Sérologie</term><term>Séronégativité</term><term>SIDA</term><term>Ghana</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 29-year-old Ghanaian woman who developed AIDS while being HIV-antibody seronegative was investigated during a collaborative study aimed at the identification of viral causes of a HIV-seronegative AIDS syndrome in West Africa. Plasma was screened with a panel of EIA tests for antibodies to HIV and HIV-1 p24 antigen. Retroviral infection was investigated by detection of reverse transcriptase (RT) activity in plasma, viral RNA amplification and quantification, and virus isolation. Positive amplification products were sequenced and phylogenetic analyses were carried out. Most EIA tests were unable to demonstrate the presence of anti-HIV antibodies, whereas confirmatory assays yielded inconclusive results. Retroviral infection was documented by detection of RT activity, HIV-1-specific genomic amplification and virus isolation. This virus was HIV-1 subtype A with an unusual six amino acid insertion in the gp120 V4 loop and with the nef gene of subtype G. The patient's plasma did not react with either autologous or heterologous viral lysates or HIV-1 peptides, whereas antibodies to other viral antigens were present. In conclusion, the Ghanaian patient exhibited a rare subtype A/G recombinant HIV-1 infection with a near absence of a HIV-specific humoral response. The lack of detectable antibody response might be due to either a highly pathogenic, rapidly fatal, HIV-1 infection preventing the development of the typical humoral immune response or to a host-related dysfunction of the immune system. Direct antigenemia or genomic detection of the virus should be undertaken when clinical or biological data suggests an HIV infection in the absence of serological evidence.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li></region><settlement><li>Cambridge</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Candotti, D" sort="Candotti, D" uniqKey="Candotti D" first="D." last="Candotti">D. Candotti</name></region><name sortKey="Allain, J P" sort="Allain, J P" uniqKey="Allain J" first="J.-P." last="Allain">J.-P. Allain</name><name sortKey="Baldrich Rubio, E" sort="Baldrich Rubio, E" uniqKey="Baldrich Rubio E" first="E." last="Baldrich-Rubio">E. Baldrich-Rubio</name><name sortKey="Davies, F" sort="Davies, F" uniqKey="Davies F" first="F." last="Davies">F. Davies</name><name sortKey="Lee, H" sort="Lee, H" uniqKey="Lee H" first="H." last="Lee">H. Lee</name><name sortKey="Stirrups, K" sort="Stirrups, K" uniqKey="Stirrups K" first="K." last="Stirrups">K. Stirrups</name></country><country name="Ghana"><noRegion><name sortKey="Adu Sarkodie, Y" sort="Adu Sarkodie, Y" uniqKey="Adu Sarkodie Y" first="Y." last="Adu-Sarkodie">Y. Adu-Sarkodie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001260 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 001260 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Main
|étape= Merge
|type= RBID
|clé= Pascal:00-0429377
|texte= AIDS in an HIV-seronegative Ghanaian woman with intersubtype A/G recombinant HIV-1 infection
}}
| This area was generated with Dilib version V0.6.31. |  |