SidaGhanaV1, Main, Exploration, bibRecord, 001322

Elucidation of an HIV-1 transmission from mother to child in West Africa by sequence analysis

Identifieur interne : 001322 ( Main/Exploration ); précédent : 001321; suivant : 001323

Elucidation of an HIV-1 transmission from mother to child in West Africa by sequence analysis

Auteurs : P. Kasper [Allemagne] ; R. Kaiser [Allemagne] ; A. Steinbeck-Klose ; B. Matz [Allemagne] ; K. E. Schneweis [Allemagne]

Source :

Zentralblatt für Bakteriologie [ 0934-8840 ] ; 1996.

RBID : Pascal:96-0343585

Descripteurs français

English descriptors

KwdEn :
- AIDS, Africa, Western, Amino Acid Sequence, Aminoacid sequence, Case study, Child, Preschool, Congenital disease, DNA, DNA Primers, Female, Follow-Up Studies, Gene Products, gag (genetics), Genes, nef, Genes, vif, HIV Antigens (genetics), HIV Envelope Protein gp120 (genetics), HIV Infections (transmission), HIV Infections (virology), HIV-1 (classification), HIV-1 (genetics), HIV-1 virus, Homology, Human, Humans, Infectious Disease Transmission, Vertical, Molecular Sequence Data, Mother to child transmission, Mothers, Newborn, Nucleotide sequence, Peptide Fragments (genetics), Phylogeny, Pregnancy, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Serotyping, Viral Proteins, gag Gene Products, Human Immunodeficiency Virus.
MESH :
- chemical , genetics : Gene Products, gag, HIV Antigens, HIV Envelope Protein gp120, Peptide Fragments.
- chemical : DNA Primers, Viral Proteins, gag Gene Products, Human Immunodeficiency Virus.
- geographic : Africa, Western.
- classification : HIV-1.
- genetics : HIV-1.
- transmission : HIV Infections.
- virology : HIV Infections.
- Amino Acid Sequence, Child, Preschool, Female, Follow-Up Studies, Genes, nef, Genes, vif, Humans, Infectious Disease Transmission, Vertical, Molecular Sequence Data, Mothers, Phylogeny, Pregnancy, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Serotyping.

Abstract

A pregnant woman living in Germany went to Ghana for several months, where she received 4 blood transfusions. Her newborn child also received one blood transfusion in West Africa. After return to Germany, HIV-1 infection was detected in both of them. Serotyping with V3 peptides revealed that the sera reacted only poorly with the subtype B-specific antigens. To investigate whether the child had been infected by vertical or parenteral transmission, we amplified different proviral HIV-1 gene segments from samples obtained 1-3 years after infection. Sequence analysis of the hypervariable regions V1 and V2 of the proviral env gene was misleading, since the viral population of the mother was highly heterogeneous, whereas only one predominant viral variant was found in the child. In contrast, sequences of the gag p17 gene and the regulatory genes nef and vif were homogeneous and revealed a very high homology, suggesting that the child had been infected by the mother. This was confirmed by phylogenetic tree analysis showing that sequences of mother and child clustered together and that both were infected by HIV-1 subtype A which is common in West Africa. The results suggest that sequence analysis of the hypervariable regions V1 and V2 alone can lead to unclear results, especially if not single genomes are analysed but a mixture of quasi-species. It is recommended that investigations into HIV transmission should be based on sequence analysis of several HIV genes.

Affiliations:

Allemagne

Le document en format XML

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<term>Aminoacid sequence</term>
<term>Case study</term>
<term>Child, Preschool</term>
<term>Congenital disease</term>
<term>DNA</term>
<term>DNA Primers</term>
<term>Female</term>
<term>Follow-Up Studies</term>
<term>Gene Products, gag (genetics)</term>
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<term>HIV Envelope Protein gp120 (genetics)</term>
<term>HIV Infections (transmission)</term>
<term>HIV Infections (virology)</term>
<term>HIV-1 (classification)</term>
<term>HIV-1 (genetics)</term>
<term>HIV-1 virus</term>
<term>Homology</term>
<term>Human</term>
<term>Humans</term>
<term>Infectious Disease Transmission, Vertical</term>
<term>Molecular Sequence Data</term>
<term>Mother to child transmission</term>
<term>Mothers</term>
<term>Newborn</term>
<term>Nucleotide sequence</term>
<term>Peptide Fragments (genetics)</term>
<term>Phylogeny</term>
<term>Pregnancy</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Serotyping</term>
<term>Viral Proteins</term>
<term>gag Gene Products, Human Immunodeficiency Virus</term>
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<term>Données de séquences moléculaires</term>
<term>Enfant d'âge préscolaire</term>
<term>Femelle</term>
<term>Fragments peptidiques (génétique)</term>
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<term>Gènes nef</term>
<term>Gènes vif</term>
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<term>Infections à VIH (transmission)</term>
<term>Infections à VIH (virologie)</term>
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<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
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<term>HIV Envelope Protein gp120</term>
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<term>Grossesse</term>
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<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">A pregnant woman living in Germany went to Ghana for several months, where she received 4 blood transfusions. Her newborn child also received one blood transfusion in West Africa. After return to Germany, HIV-1 infection was detected in both of them. Serotyping with V3 peptides revealed that the sera reacted only poorly with the subtype B-specific antigens. To investigate whether the child had been infected by vertical or parenteral transmission, we amplified different proviral HIV-1 gene segments from samples obtained 1-3 years after infection. Sequence analysis of the hypervariable regions V1 and V2 of the proviral env gene was misleading, since the viral population of the mother was highly heterogeneous, whereas only one predominant viral variant was found in the child. In contrast, sequences of the gag p17 gene and the regulatory genes nef and vif were homogeneous and revealed a very high homology, suggesting that the child had been infected by the mother. This was confirmed by phylogenetic tree analysis showing that sequences of mother and child clustered together and that both were infected by HIV-1 subtype A which is common in West Africa. The results suggest that sequence analysis of the hypervariable regions V1 and V2 alone can lead to unclear results, especially if not single genomes are analysed but a mixture of quasi-species. It is recommended that investigations into HIV transmission should be based on sequence analysis of several HIV genes.</div>
</front>
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Le SIDA au Ghana (serveur d'exploration)

Elucidation of an HIV-1 transmission from mother to child in West Africa by sequence analysis

Elucidation of an HIV-1 transmission from mother to child in West Africa by sequence analysis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

	Le SIDA au Ghana (serveur d'exploration)
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