Reactivity of a new HIV-1 group O third generation A-HIV-1/-2 assay with an unusual HIV-1 seroconversion panel and HIV-1 group O/group M subtyped samples
Identifieur interne : 001263 ( Main/Exploration ); précédent : 001262; suivant : 001264Reactivity of a new HIV-1 group O third generation A-HIV-1/-2 assay with an unusual HIV-1 seroconversion panel and HIV-1 group O/group M subtyped samples
Auteurs : J. Van Binsbergen [Pays-Bas] ; W. Keur [Pays-Bas] ; M. V. D. Graaf [Pays-Bas] ; A. Siebelink [Pays-Bas] ; A. Jacobs [Pays-Bas] ; D. De Rijk [Pays-Bas] ; J. Toonen [Pays-Bas] ; L. Zekeng [Allemagne] ; E. Afane Ze [Allemagne] ; L. G Gürtler [Allemagne]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1997.
Descripteurs français
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Amino acids, Asian countries, Assay, Binsbergen, Blood bank, Clinical samples, Competitive elisa, Consensus sequences, Elisa, Ghana, Group antigen, Group samples, Gurtler, Histidine residue, Human virus, Immunodominant region, Indirect elisa, Loop peptides, Organon teknika, Other assays, Other seroconversion panels, Other strains, Peptide, Performance panel, Reactive, Reactive samples, Same donor, Seroconversion, Seroconversion panel, Seroconversion panels, Subtype, Subtype samples, Subtyped samples, Third generation assay, Unusual seroconversion panel, Vanden haesevelde, Virological, Virological methods, Vironostika.
- Teeft :
- Amino acids, Asian countries, Assay, Binsbergen, Blood bank, Clinical samples, Competitive elisa, Consensus sequences, Elisa, Ghana, Group antigen, Group samples, Gurtler, Histidine residue, Human virus, Immunodominant region, Indirect elisa, Loop peptides, Organon teknika, Other assays, Other seroconversion panels, Other strains, Peptide, Performance panel, Reactive, Reactive samples, Same donor, Seroconversion, Seroconversion panel, Seroconversion panels, Subtype, Subtype samples, Subtyped samples, Third generation assay, Unusual seroconversion panel, Vanden haesevelde, Virological, Virological methods, Vironostika.
Abstract
It was shown previously that about 97% of the anti-HIV-1 group O strain-positive samples were detected by crossreaction with native HIV-1 gp160 (Van Binsbergen et al., Evaluation of a new third generation anti-HIV-1/anti-HIV-2 assay with increased sensitivity for HIV-1 group O, J. Virol. Methods 60 (1996) 131–137). Fourteen out of 17 new anti-HIV-1 group O positive samples, selected with the Enzygnost HIV-1/2 plus assay, were already reactive when tested with HIV-1 gp160. When tested by the Vironostika HIV Uni-Form II plus O microELISA all 17 samples were reactive, demonstrating the necessity to implement an HIV-1 group O-specific antigen in the assay. On the other hand, it was surprisingly found that 40 out of 43 (93%) of anti-HIV-1 group M-positive samples, belonging to strain A, B, C, D, E or F, were detected by crossreaction with the HIV-1 group O (strain ANT70) synthetic peptide incorporated in the Vironostika HIV Uni-Form II plus O. Only HIV-1 subtype D-positive samples did not react with this peptide, presumably because of the presence of a histidine residue in the immunodominant region of HIV-1 subtype D gp41. Both crossreactions make the Vironostika HIV Uni-Form II plus O microELISA also sensitive for anti-HIV-1-positive samples originating from different geographical regions and resulting from different HIV-1 subtype infections. With an unusual seroconversion panel in which p24 Ag was present persistently, many anti-HIV-1/-2 assays produce alternating positive/negative results in anti-HIV antibody-positive bleeds. It was shown that the use of viral p24 and gp160 in a direct sandwich, allowing detection of anti-HIV IgG and IgM, explains the identification of all anti-HIV-positive bleeds by the Vironostika HIV Uni-Form II plus O. The high sensitivity of the plus O assay was confirmed with clinical samples of a so-called anti-HIV-1 low titer panel. The specificity of the Vironostika HIV Uni-Form II plus O determined in five blood transfusion centers, based on 135 070 tests, was 99.97%.
Url:
DOI: 10.1016/S0166-0934(97)00135-3
Affiliations:
- Allemagne, Pays-Bas
- Bavière, District de Haute-Bavière
- Munich
- Université Louis-et-Maximilien de Munich
Links toward previous steps (curation, corpus...)
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Le document en format XML
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<term>Clinical samples</term>
<term>Competitive elisa</term>
<term>Consensus sequences</term>
<term>Elisa</term>
<term>Ghana</term>
<term>Group antigen</term>
<term>Group samples</term>
<term>Gurtler</term>
<term>Histidine residue</term>
<term>Human virus</term>
<term>Immunodominant region</term>
<term>Indirect elisa</term>
<term>Loop peptides</term>
<term>Organon teknika</term>
<term>Other assays</term>
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<term>Other strains</term>
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<term>Reactive samples</term>
<term>Same donor</term>
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<term>Seroconversion panel</term>
<term>Seroconversion panels</term>
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<term>Unusual seroconversion panel</term>
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<front><div type="abstract" xml:lang="en">It was shown previously that about 97% of the anti-HIV-1 group O strain-positive samples were detected by crossreaction with native HIV-1 gp160 (Van Binsbergen et al., Evaluation of a new third generation anti-HIV-1/anti-HIV-2 assay with increased sensitivity for HIV-1 group O, J. Virol. Methods 60 (1996) 131–137). Fourteen out of 17 new anti-HIV-1 group O positive samples, selected with the Enzygnost HIV-1/2 plus assay, were already reactive when tested with HIV-1 gp160. When tested by the Vironostika HIV Uni-Form II plus O microELISA all 17 samples were reactive, demonstrating the necessity to implement an HIV-1 group O-specific antigen in the assay. On the other hand, it was surprisingly found that 40 out of 43 (93%) of anti-HIV-1 group M-positive samples, belonging to strain A, B, C, D, E or F, were detected by crossreaction with the HIV-1 group O (strain ANT70) synthetic peptide incorporated in the Vironostika HIV Uni-Form II plus O. Only HIV-1 subtype D-positive samples did not react with this peptide, presumably because of the presence of a histidine residue in the immunodominant region of HIV-1 subtype D gp41. Both crossreactions make the Vironostika HIV Uni-Form II plus O microELISA also sensitive for anti-HIV-1-positive samples originating from different geographical regions and resulting from different HIV-1 subtype infections. With an unusual seroconversion panel in which p24 Ag was present persistently, many anti-HIV-1/-2 assays produce alternating positive/negative results in anti-HIV antibody-positive bleeds. It was shown that the use of viral p24 and gp160 in a direct sandwich, allowing detection of anti-HIV IgG and IgM, explains the identification of all anti-HIV-positive bleeds by the Vironostika HIV Uni-Form II plus O. The high sensitivity of the plus O assay was confirmed with clinical samples of a so-called anti-HIV-1 low titer panel. The specificity of the Vironostika HIV Uni-Form II plus O determined in five blood transfusion centers, based on 135 070 tests, was 99.97%.</div>
</front>
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