A cluster of human immunodeficiency virus Type 1 recombinant form escaping detection by commercial genomic amplification assays
Identifieur interne : 000988 ( Istex/Curation ); précédent : 000987; suivant : 000989A cluster of human immunodeficiency virus Type 1 recombinant form escaping detection by commercial genomic amplification assays
Auteurs : Barbara Foglieni [Royaume-Uni] ; Daniel Candotti [Royaume-Uni] ; Irene Guarnori [Royaume-Uni] ; Livia Raffaele [Royaume-Uni] ; Alessandra Berzuini [Royaume-Uni] ; Marta Spreafico [Royaume-Uni] ; Anna Orani [Royaume-Uni] ; Roberto Rossotti [Royaume-Uni] ; Davide Rossi [Royaume-Uni] ; Jean-Pierre Allain [Royaume-Uni] ; Daniele Prati [Royaume-Uni]Source :
- Transfusion [ 0041-1132 ] ; 2011-04.
Descripteurs français
- Wicri :
- topic : Don.
English descriptors
- KwdEn :
- Abbott architect combo assay, Active antiretroviral therapy, Agilent, Antiretroviral treatment, April, Assay, Barbara foglieni, Base variations, Blood donation, Blood donors, Blood supply, Bootstrap support, Clin, Clin microbiol, Clin virol, Cobas, Common source, Diagnostic assays, Diagnostics, Different regions, Discordant results, Donation, Foglieni, Genbank accession number, Genetic diversity, Genetic subtypes, Genome, Genome sequences, Genome sequencing, Genotyped reference sequences, Heterosexual, Heterosexual couples, Human virus type, Index blood donation, Index case, Index donation, Index strain, Index strain sequence, Index subject, Inner primers, Intersubtype recombination, Lymphocyte subpopulation counts, Mismatch, Online version, Other assays, Other crfs, Other regions, Phylogenetic, Phylogenetic analysis, Phylogenetic tree, Plasma samples, Poor performance, Primary infection, Primer, Primer sets, Qpcr, Quantitative assay, Quantitative assays, Recombinant, Recombinant forms, Recombinant sequences, Recombinant strain, Recombinant strains, Reference sequence, Reference sequences, Region sequences, Replicates, Roche, Roche assays, Roche diagnostics, Roche newgeneration, Roche primers, Roche qpcr, Roche roche roche, Roche systems, Routine assay, Same strain, Screening assay, Sequencing, Siemens, Similar pattern, Sinauer associates, Subtype, Subtypes, Test cobas, Test cobas ampliscreen, Transfusion, Transfusion medicine, Transfusion volume, Viral, Viral load, Viral loads, Viral particles, Viremia, Virol, Virol methods, Virus type.
- Teeft :
- Abbott architect combo assay, Active antiretroviral therapy, Agilent, Antiretroviral treatment, April, Assay, Barbara foglieni, Base variations, Blood donation, Blood donors, Blood supply, Bootstrap support, Clin, Clin microbiol, Clin virol, Cobas, Common source, Diagnostic assays, Diagnostics, Different regions, Discordant results, Donation, Foglieni, Genbank accession number, Genetic diversity, Genetic subtypes, Genome, Genome sequences, Genome sequencing, Genotyped reference sequences, Heterosexual, Heterosexual couples, Human virus type, Index blood donation, Index case, Index donation, Index strain, Index strain sequence, Index subject, Inner primers, Intersubtype recombination, Lymphocyte subpopulation counts, Mismatch, Online version, Other assays, Other crfs, Other regions, Phylogenetic, Phylogenetic analysis, Phylogenetic tree, Plasma samples, Poor performance, Primary infection, Primer, Primer sets, Qpcr, Quantitative assay, Quantitative assays, Recombinant, Recombinant forms, Recombinant sequences, Recombinant strain, Recombinant strains, Reference sequence, Reference sequences, Region sequences, Replicates, Roche, Roche assays, Roche diagnostics, Roche newgeneration, Roche primers, Roche qpcr, Roche roche roche, Roche systems, Routine assay, Same strain, Screening assay, Sequencing, Siemens, Similar pattern, Sinauer associates, Subtype, Subtypes, Test cobas, Test cobas ampliscreen, Transfusion, Transfusion medicine, Transfusion volume, Viral, Viral load, Viral loads, Viral particles, Viremia, Virol, Virol methods, Virus type.
Abstract
BACKGROUND: Nucleic acid testing (NAT)‐based methods for the detection and quantification of human immunodeficiency virus Type 1 (HIV‐1) RNA are used to increase transfusion safety and to diagnose and manage HIV‐1‐infected patients. We describe a novel HIV‐1 recombinant form associated with lack of reactivity or substantial underestimation of viral load by commercial NAT assays.
Url:
DOI: 10.1111/j.1537-2995.2010.02942.x
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ISTEX:7CA4F4A59DFC48E4ABF149869CF3B04FD06BAAD5Le document en format XML
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<author><name sortKey="Foglieni, Barbara" sort="Foglieni, Barbara" uniqKey="Foglieni B" first="Barbara" last="Foglieni">Barbara Foglieni</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
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<author><name sortKey="Candotti, Daniel" sort="Candotti, Daniel" uniqKey="Candotti D" first="Daniel" last="Candotti">Daniel Candotti</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
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<author><name sortKey="Guarnori, Irene" sort="Guarnori, Irene" uniqKey="Guarnori I" first="Irene" last="Guarnori">Irene Guarnori</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
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<author><name sortKey="Raffaele, Livia" sort="Raffaele, Livia" uniqKey="Raffaele L" first="Livia" last="Raffaele">Livia Raffaele</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Berzuini, Alessandra" sort="Berzuini, Alessandra" uniqKey="Berzuini A" first="Alessandra" last="Berzuini">Alessandra Berzuini</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Spreafico, Marta" sort="Spreafico, Marta" uniqKey="Spreafico M" first="Marta" last="Spreafico">Marta Spreafico</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Orani, Anna" sort="Orani, Anna" uniqKey="Orani A" first="Anna" last="Orani">Anna Orani</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rossotti, Roberto" sort="Rossotti, Roberto" uniqKey="Rossotti R" first="Roberto" last="Rossotti">Roberto Rossotti</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
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<author><name sortKey="Rossi, Davide" sort="Rossi, Davide" uniqKey="Rossi D" first="Davide" last="Rossi">Davide Rossi</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Prati, Daniele" sort="Prati, Daniele" uniqKey="Prati D" first="Daniele" last="Prati">Daniele Prati</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
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<sourceDesc><biblStruct><analytic><title level="a" type="main">A cluster of human immunodeficiency virus Type 1 recombinant form escaping detection by commercial genomic amplification assays</title>
<author><name sortKey="Foglieni, Barbara" sort="Foglieni, Barbara" uniqKey="Foglieni B" first="Barbara" last="Foglieni">Barbara Foglieni</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Candotti, Daniel" sort="Candotti, Daniel" uniqKey="Candotti D" first="Daniel" last="Candotti">Daniel Candotti</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Guarnori, Irene" sort="Guarnori, Irene" uniqKey="Guarnori I" first="Irene" last="Guarnori">Irene Guarnori</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Raffaele, Livia" sort="Raffaele, Livia" uniqKey="Raffaele L" first="Livia" last="Raffaele">Livia Raffaele</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Berzuini, Alessandra" sort="Berzuini, Alessandra" uniqKey="Berzuini A" first="Alessandra" last="Berzuini">Alessandra Berzuini</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Spreafico, Marta" sort="Spreafico, Marta" uniqKey="Spreafico M" first="Marta" last="Spreafico">Marta Spreafico</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Orani, Anna" sort="Orani, Anna" uniqKey="Orani A" first="Anna" last="Orani">Anna Orani</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rossotti, Roberto" sort="Rossotti, Roberto" uniqKey="Rossotti R" first="Roberto" last="Rossotti">Roberto Rossotti</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Rossi, Davide" sort="Rossi, Davide" uniqKey="Rossi D" first="Davide" last="Rossi">Davide Rossi</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Allain, Jean Ierre" sort="Allain, Jean Ierre" uniqKey="Allain J" first="Jean-Pierre" last="Allain">Jean-Pierre Allain</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Prati, Daniele" sort="Prati, Daniele" uniqKey="Prati D" first="Daniele" last="Prati">Daniele Prati</name>
<affiliation wicri:level="1"><mods:affiliation>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge, UK.</mods:affiliation>
<country xml:lang="fr" wicri:curation="lc">Royaume-Uni</country>
<wicri:regionArea>From the Department of Transfusion Medicine and Hematology and Infectious Diseases Unit, Ospedale A. Manzoni, Lecco, Italy; Centro Trasfusionale Ospedale di Circolo di Varese, Varese, Italy; Center of Transfusion Medicine, Cellular Therapy and Cryobiology, IRCCS Foundation Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; National Health Service Blood & Transplant, Cambridge Blood Centre, Cambridge, UK; and the Department of Hematology, University of Cambridge, Cambridge</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Transfusion</title>
<title level="j" type="alt">TRANSFUSION</title>
<idno type="ISSN">0041-1132</idno>
<idno type="eISSN">1537-2995</idno>
<imprint><biblScope unit="vol">51</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="719">719</biblScope>
<biblScope unit="page" to="730">730</biblScope>
<biblScope unit="page-count">12</biblScope>
<publisher>Blackwell Publishing Inc</publisher>
<pubPlace>Malden, USA</pubPlace>
<date type="published" when="2011-04">2011-04</date>
</imprint>
<idno type="ISSN">0041-1132</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0041-1132</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abbott architect combo assay</term>
<term>Active antiretroviral therapy</term>
<term>Agilent</term>
<term>Antiretroviral treatment</term>
<term>April</term>
<term>Assay</term>
<term>Barbara foglieni</term>
<term>Base variations</term>
<term>Blood donation</term>
<term>Blood donors</term>
<term>Blood supply</term>
<term>Bootstrap support</term>
<term>Clin</term>
<term>Clin microbiol</term>
<term>Clin virol</term>
<term>Cobas</term>
<term>Common source</term>
<term>Diagnostic assays</term>
<term>Diagnostics</term>
<term>Different regions</term>
<term>Discordant results</term>
<term>Donation</term>
<term>Foglieni</term>
<term>Genbank accession number</term>
<term>Genetic diversity</term>
<term>Genetic subtypes</term>
<term>Genome</term>
<term>Genome sequences</term>
<term>Genome sequencing</term>
<term>Genotyped reference sequences</term>
<term>Heterosexual</term>
<term>Heterosexual couples</term>
<term>Human virus type</term>
<term>Index blood donation</term>
<term>Index case</term>
<term>Index donation</term>
<term>Index strain</term>
<term>Index strain sequence</term>
<term>Index subject</term>
<term>Inner primers</term>
<term>Intersubtype recombination</term>
<term>Lymphocyte subpopulation counts</term>
<term>Mismatch</term>
<term>Online version</term>
<term>Other assays</term>
<term>Other crfs</term>
<term>Other regions</term>
<term>Phylogenetic</term>
<term>Phylogenetic analysis</term>
<term>Phylogenetic tree</term>
<term>Plasma samples</term>
<term>Poor performance</term>
<term>Primary infection</term>
<term>Primer</term>
<term>Primer sets</term>
<term>Qpcr</term>
<term>Quantitative assay</term>
<term>Quantitative assays</term>
<term>Recombinant</term>
<term>Recombinant forms</term>
<term>Recombinant sequences</term>
<term>Recombinant strain</term>
<term>Recombinant strains</term>
<term>Reference sequence</term>
<term>Reference sequences</term>
<term>Region sequences</term>
<term>Replicates</term>
<term>Roche</term>
<term>Roche assays</term>
<term>Roche diagnostics</term>
<term>Roche newgeneration</term>
<term>Roche primers</term>
<term>Roche qpcr</term>
<term>Roche roche roche</term>
<term>Roche systems</term>
<term>Routine assay</term>
<term>Same strain</term>
<term>Screening assay</term>
<term>Sequencing</term>
<term>Siemens</term>
<term>Similar pattern</term>
<term>Sinauer associates</term>
<term>Subtype</term>
<term>Subtypes</term>
<term>Test cobas</term>
<term>Test cobas ampliscreen</term>
<term>Transfusion</term>
<term>Transfusion medicine</term>
<term>Transfusion volume</term>
<term>Viral</term>
<term>Viral load</term>
<term>Viral loads</term>
<term>Viral particles</term>
<term>Viremia</term>
<term>Virol</term>
<term>Virol methods</term>
<term>Virus type</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Abbott architect combo assay</term>
<term>Active antiretroviral therapy</term>
<term>Agilent</term>
<term>Antiretroviral treatment</term>
<term>April</term>
<term>Assay</term>
<term>Barbara foglieni</term>
<term>Base variations</term>
<term>Blood donation</term>
<term>Blood donors</term>
<term>Blood supply</term>
<term>Bootstrap support</term>
<term>Clin</term>
<term>Clin microbiol</term>
<term>Clin virol</term>
<term>Cobas</term>
<term>Common source</term>
<term>Diagnostic assays</term>
<term>Diagnostics</term>
<term>Different regions</term>
<term>Discordant results</term>
<term>Donation</term>
<term>Foglieni</term>
<term>Genbank accession number</term>
<term>Genetic diversity</term>
<term>Genetic subtypes</term>
<term>Genome</term>
<term>Genome sequences</term>
<term>Genome sequencing</term>
<term>Genotyped reference sequences</term>
<term>Heterosexual</term>
<term>Heterosexual couples</term>
<term>Human virus type</term>
<term>Index blood donation</term>
<term>Index case</term>
<term>Index donation</term>
<term>Index strain</term>
<term>Index strain sequence</term>
<term>Index subject</term>
<term>Inner primers</term>
<term>Intersubtype recombination</term>
<term>Lymphocyte subpopulation counts</term>
<term>Mismatch</term>
<term>Online version</term>
<term>Other assays</term>
<term>Other crfs</term>
<term>Other regions</term>
<term>Phylogenetic</term>
<term>Phylogenetic analysis</term>
<term>Phylogenetic tree</term>
<term>Plasma samples</term>
<term>Poor performance</term>
<term>Primary infection</term>
<term>Primer</term>
<term>Primer sets</term>
<term>Qpcr</term>
<term>Quantitative assay</term>
<term>Quantitative assays</term>
<term>Recombinant</term>
<term>Recombinant forms</term>
<term>Recombinant sequences</term>
<term>Recombinant strain</term>
<term>Recombinant strains</term>
<term>Reference sequence</term>
<term>Reference sequences</term>
<term>Region sequences</term>
<term>Replicates</term>
<term>Roche</term>
<term>Roche assays</term>
<term>Roche diagnostics</term>
<term>Roche newgeneration</term>
<term>Roche primers</term>
<term>Roche qpcr</term>
<term>Roche roche roche</term>
<term>Roche systems</term>
<term>Routine assay</term>
<term>Same strain</term>
<term>Screening assay</term>
<term>Sequencing</term>
<term>Siemens</term>
<term>Similar pattern</term>
<term>Sinauer associates</term>
<term>Subtype</term>
<term>Subtypes</term>
<term>Test cobas</term>
<term>Test cobas ampliscreen</term>
<term>Transfusion</term>
<term>Transfusion medicine</term>
<term>Transfusion volume</term>
<term>Viral</term>
<term>Viral load</term>
<term>Viral loads</term>
<term>Viral particles</term>
<term>Viremia</term>
<term>Virol</term>
<term>Virol methods</term>
<term>Virus type</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Don</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">BACKGROUND: Nucleic acid testing (NAT)‐based methods for the detection and quantification of human immunodeficiency virus Type 1 (HIV‐1) RNA are used to increase transfusion safety and to diagnose and manage HIV‐1‐infected patients. We describe a novel HIV‐1 recombinant form associated with lack of reactivity or substantial underestimation of viral load by commercial NAT assays.</div>
</front>
</TEI>
</record>
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