Archetype JC virus efficiently propagates in kidney‐derived cells stably expressing HIV‐1 Tat
Identifieur interne : 000972 ( Istex/Curation ); précédent : 000971; suivant : 000973Archetype JC virus efficiently propagates in kidney‐derived cells stably expressing HIV‐1 Tat
Auteurs : Souichi Nukuzuma ; Masanori Kameoka [Japon] ; Shigeki Sugiura ; Kazuo Nakamichi ; Chiyoko Nukuzuma ; Isao Miyoshi ; Tsutomu Takegami [Japon]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2009-11.
English descriptors
- KwdEn :
- Actin mrna, Aids patients, Antigen expression, Antigen mrna, Archetype, Assay, Blackwell publishing asia, Blood center, Cell clones, Cell lines, Cell lines stably, Cell monolayer, Cells stably, Cells transfected, Cells untransfected, Clin microbiol, Clone, Complete growth medium, Copy numbers, Culture medium, Culture system, Cultured cells, Demyelinating disease, Detectable level, Epithelial cells, Etiologic agent, Expression vector, Galactosidase activity, Genome replication, Glial cells, High incidence, Human polyomavirus, Human virus type, Hypervariable sequences, Immunocompromised patients, Immunocytochemical staining, Jurkat cells, Kobe institute, Late promoter, Lipofectamine reagent, Luciferase, Luciferase activity, Microbiol immunol, Mrna, Mrna expression, National center, Nukuzuma, Other hand, Parental cells, Persistent infection, Plasmid, Previous report, Primer, Proc natl acad, Progeny viruses, Progressive multifocal leukoencephalopathy, Promoter, Regulatory region, Replication, Reporter gene, Reporter gene assay, Results show, Simian, Simian virus, Split ratio, Stably, Strong stimulator, Taqman probe, Transfected, Transfected cells, Transfection, Untransfected cells, Useful tool, Virol, Virus propagation, Yogo.
- Teeft :
- Actin mrna, Aids patients, Antigen expression, Antigen mrna, Archetype, Assay, Blackwell publishing asia, Blood center, Cell clones, Cell lines, Cell lines stably, Cell monolayer, Cells stably, Cells transfected, Cells untransfected, Clin microbiol, Clone, Complete growth medium, Copy numbers, Culture medium, Culture system, Cultured cells, Demyelinating disease, Detectable level, Epithelial cells, Etiologic agent, Expression vector, Galactosidase activity, Genome replication, Glial cells, High incidence, Human polyomavirus, Human virus type, Hypervariable sequences, Immunocompromised patients, Immunocytochemical staining, Jurkat cells, Kobe institute, Late promoter, Lipofectamine reagent, Luciferase, Luciferase activity, Microbiol immunol, Mrna, Mrna expression, National center, Nukuzuma, Other hand, Parental cells, Persistent infection, Plasmid, Previous report, Primer, Proc natl acad, Progeny viruses, Progressive multifocal leukoencephalopathy, Promoter, Regulatory region, Replication, Reporter gene, Reporter gene assay, Results show, Simian, Simian virus, Split ratio, Stably, Strong stimulator, Taqman probe, Transfected, Transfected cells, Transfection, Untransfected cells, Useful tool, Virol, Virus propagation, Yogo.
Abstract
Pathogenic JCV with rearranged regulatory regions (PML‐type) causes PML, a demyelinating disease, in the brains of immunocompromised patients. On the other hand, archetype JCV persistently infecting the kidney is thought to be converted to PML‐type virus during JCV replication in the infected host under immunosuppressed conditions. In addition, Tat protein, encoded by HIV‐1, markedly enhances the expression of a reporter gene under control of the JCV late promoter. In order to examine the influence of Tat on JCV propagation, we used kidney‐derived COS‐7 cells, which only permit archetype JCV, and established COS‐tat cells, which express HIV‐1 Tat stably. We found that the extent of archetype JCV propagation in COS‐tat cells is significantly greater than in COS‐7 cells. On the other hand, COS‐7 cells express SV40 T antigen, which is a strong stimulator of archetype JCV replication. The expression of SV40 T antigen was enhanced by HIV‐1 Tat slightly according to real‐time RT‐PCR, this was not closely related to JCV replication in COS‐tat cells. The efficiency of JCV propagation depended on the extent of expression of functional Tat. To our knowledge, this is the first report of increased production of archetype JCV in a culture system using cell lines stably expressing HIV‐1 Tat. We propose here that COS‐tat cells are a useful tool for studying the role of Tat in archetype JCV replication in the development of PML.
Url:
DOI: 10.1111/j.1348-0421.2009.00166.x
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Souichi Nukuzuma<affiliation><mods:affiliation>Department of Microbiology, Kobe Institute of Health, 4‐6, Minatojima‐Nakamachi, Chuo‐ku, Kobe, Hyogo 650‐0046</mods:affiliation><wicri:noCountry code="subField">650‐0046</wicri:noCountry></affiliation><affiliation><mods:affiliation>Medical Genetics Research Center, Nara Medical University, Kashihara, Nara 634‐8521</mods:affiliation><wicri:noCountry code="subField">634‐8521</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku, Tokyo 162‐8640</mods:affiliation><wicri:noCountry code="subField">162‐8640</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Microbiology, Kobe Institute of Health, 4‐6, Minatojima‐Nakamachi, Chuo‐ku, Kobe, Hyogo 650‐0046</mods:affiliation><wicri:noCountry code="subField">650‐0046</wicri:noCountry></affiliation><affiliation><mods:affiliation>Department of Medicine, Kochi Medical School, Kohasu, Oko‐cho, Nankoku, Kochi 783‐8505</mods:affiliation><wicri:noCountry code="subField">783‐8505</wicri:noCountry></affiliation>Le document en format XML
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Emerging and Re‐emerging Infections (RCC‐ERI), Research Institute for Microbial Diseases, Osaka University, Nonthaburi 11000, Thailand.Souichi Nukuzuma, Department of Microbiology, Kobe Institute of Health, 4‐6, Minatojima‐Nakamachi, Chuo‐ku, Kobe, Hyogo 650‐0046, Japan.Tel: +81 78 302 6256; fax: +81 78 302 0894; email:</mods:affiliation><wicri:noCountry code="subField">email:</wicri:noCountry></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: s‐nuku@gj8.so‐net.ne.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Sugiura, Shigeki" sort="Sugiura, Shigeki" uniqKey="Sugiura S" first="Shigeki" last="Sugiura">Shigeki Sugiura</name><affiliation><mods:affiliation>Medical Genetics Research Center, Nara Medical University, Kashihara, Nara 634‐8521</mods:affiliation><wicri:noCountry code="subField">634‐8521</wicri:noCountry></affiliation></author><author><name sortKey="Nakamichi, Kazuo" sort="Nakamichi, Kazuo" 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Biochemistry</mods:affiliation></affiliation><affiliation><mods:affiliation>†Present address: Thailand‐Japan Research Collaboration Center on Emerging and Re‐emerging Infections (RCC‐ERI), Research Institute for Microbial Diseases, Osaka University, Nonthaburi 11000, Thailand.Souichi Nukuzuma, Department of Microbiology, Kobe Institute of Health, 4‐6, Minatojima‐Nakamachi, Chuo‐ku, Kobe, Hyogo 650‐0046, Japan.Tel: +81 78 302 6256; fax: +81 78 302 0894; email:</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: s‐nuku@gj8.so‐net.ne.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Sugiura, Shigeki" sort="Sugiura, Shigeki" uniqKey="Sugiura S" first="Shigeki" last="Sugiura">Shigeki Sugiura</name><affiliation><mods:affiliation>Medical Genetics Research Center, Nara Medical University, Kashihara, Nara 634‐8521</mods:affiliation><wicri:noCountry code="subField">634‐8521</wicri:noCountry></affiliation></author><author><name sortKey="Nakamichi, Kazuo" sort="Nakamichi, Kazuo" uniqKey="Nakamichi K" first="Kazuo" last="Nakamichi">Kazuo Nakamichi</name><affiliation><mods:affiliation>Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku, Tokyo 162‐8640</mods:affiliation><wicri:noCountry code="subField">162‐8640</wicri:noCountry></affiliation></author><author><name sortKey="Nukuzuma, Chiyoko" sort="Nukuzuma, Chiyoko" uniqKey="Nukuzuma C" first="Chiyoko" last="Nukuzuma">Chiyoko Nukuzuma</name><affiliation><mods:affiliation>Department of Microbiology, Kobe Institute of Health, 4‐6, Minatojima‐Nakamachi, Chuo‐ku, Kobe, Hyogo 650‐0046</mods:affiliation><wicri:noCountry code="subField">650‐0046</wicri:noCountry></affiliation></author><author><name sortKey="Miyoshi, Isao" sort="Miyoshi, Isao" uniqKey="Miyoshi I" first="Isao" last="Miyoshi">Isao Miyoshi</name><affiliation><mods:affiliation>Department of Medicine, Kochi Medical School, Kohasu, Oko‐cho, Nankoku, Kochi 783‐8505</mods:affiliation><wicri:noCountry code="subField">783‐8505</wicri:noCountry></affiliation></author><author><name sortKey="Takegami, Tsutomu" sort="Takegami, Tsutomu" uniqKey="Takegami T" first="Tsutomu" last="Takegami">Tsutomu Takegami</name><affiliation wicri:level="1"><mods:affiliation>Division of Molecular Oncology and Virology, Medical Research Institute, Kanazawa Medical University, Ishikawa 920‐0293, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular Oncology and Virology, Medical Research Institute, Kanazawa Medical University, Ishikawa 920‐0293</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Microbiology and Immunology</title><title level="j" type="alt">MICROBIOLOGY IMMUNOLOGY</title><idno type="ISSN">0385-5600</idno><idno type="eISSN">1348-0421</idno><imprint><biblScope unit="vol">53</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="621">621</biblScope><biblScope unit="page" to="628">628</biblScope><biblScope unit="page-count">8</biblScope><publisher>Blackwell Publishing Asia</publisher><pubPlace>Melbourne, Australia</pubPlace><date type="published" when="2009-11">2009-11</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actin mrna</term><term>Aids patients</term><term>Antigen expression</term><term>Antigen mrna</term><term>Archetype</term><term>Assay</term><term>Blackwell publishing asia</term><term>Blood center</term><term>Cell clones</term><term>Cell lines</term><term>Cell lines stably</term><term>Cell monolayer</term><term>Cells stably</term><term>Cells transfected</term><term>Cells untransfected</term><term>Clin microbiol</term><term>Clone</term><term>Complete growth medium</term><term>Copy numbers</term><term>Culture medium</term><term>Culture system</term><term>Cultured cells</term><term>Demyelinating disease</term><term>Detectable level</term><term>Epithelial cells</term><term>Etiologic agent</term><term>Expression vector</term><term>Galactosidase activity</term><term>Genome replication</term><term>Glial cells</term><term>High incidence</term><term>Human polyomavirus</term><term>Human virus type</term><term>Hypervariable sequences</term><term>Immunocompromised patients</term><term>Immunocytochemical staining</term><term>Jurkat cells</term><term>Kobe institute</term><term>Late promoter</term><term>Lipofectamine reagent</term><term>Luciferase</term><term>Luciferase activity</term><term>Microbiol immunol</term><term>Mrna</term><term>Mrna expression</term><term>National center</term><term>Nukuzuma</term><term>Other hand</term><term>Parental cells</term><term>Persistent infection</term><term>Plasmid</term><term>Previous report</term><term>Primer</term><term>Proc natl acad</term><term>Progeny viruses</term><term>Progressive multifocal leukoencephalopathy</term><term>Promoter</term><term>Regulatory region</term><term>Replication</term><term>Reporter gene</term><term>Reporter gene assay</term><term>Results show</term><term>Simian</term><term>Simian virus</term><term>Split ratio</term><term>Stably</term><term>Strong stimulator</term><term>Taqman probe</term><term>Transfected</term><term>Transfected cells</term><term>Transfection</term><term>Untransfected cells</term><term>Useful tool</term><term>Virol</term><term>Virus propagation</term><term>Yogo</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actin mrna</term><term>Aids patients</term><term>Antigen expression</term><term>Antigen mrna</term><term>Archetype</term><term>Assay</term><term>Blackwell publishing asia</term><term>Blood center</term><term>Cell clones</term><term>Cell lines</term><term>Cell lines stably</term><term>Cell monolayer</term><term>Cells stably</term><term>Cells transfected</term><term>Cells untransfected</term><term>Clin microbiol</term><term>Clone</term><term>Complete growth medium</term><term>Copy numbers</term><term>Culture medium</term><term>Culture system</term><term>Cultured cells</term><term>Demyelinating disease</term><term>Detectable level</term><term>Epithelial cells</term><term>Etiologic agent</term><term>Expression vector</term><term>Galactosidase activity</term><term>Genome replication</term><term>Glial cells</term><term>High incidence</term><term>Human polyomavirus</term><term>Human virus type</term><term>Hypervariable sequences</term><term>Immunocompromised patients</term><term>Immunocytochemical staining</term><term>Jurkat cells</term><term>Kobe institute</term><term>Late promoter</term><term>Lipofectamine reagent</term><term>Luciferase</term><term>Luciferase activity</term><term>Microbiol immunol</term><term>Mrna</term><term>Mrna expression</term><term>National center</term><term>Nukuzuma</term><term>Other hand</term><term>Parental cells</term><term>Persistent infection</term><term>Plasmid</term><term>Previous report</term><term>Primer</term><term>Proc natl acad</term><term>Progeny viruses</term><term>Progressive multifocal leukoencephalopathy</term><term>Promoter</term><term>Regulatory region</term><term>Replication</term><term>Reporter gene</term><term>Reporter gene assay</term><term>Results show</term><term>Simian</term><term>Simian virus</term><term>Split ratio</term><term>Stably</term><term>Strong stimulator</term><term>Taqman probe</term><term>Transfected</term><term>Transfected cells</term><term>Transfection</term><term>Untransfected cells</term><term>Useful tool</term><term>Virol</term><term>Virus propagation</term><term>Yogo</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pathogenic JCV with rearranged regulatory regions (PML‐type) causes PML, a demyelinating disease, in the brains of immunocompromised patients. On the other hand, archetype JCV persistently infecting the kidney is thought to be converted to PML‐type virus during JCV replication in the infected host under immunosuppressed conditions. In addition, Tat protein, encoded by HIV‐1, markedly enhances the expression of a reporter gene under control of the JCV late promoter. In order to examine the influence of Tat on JCV propagation, we used kidney‐derived COS‐7 cells, which only permit archetype JCV, and established COS‐tat cells, which express HIV‐1 Tat stably. We found that the extent of archetype JCV propagation in COS‐tat cells is significantly greater than in COS‐7 cells. On the other hand, COS‐7 cells express SV40 T antigen, which is a strong stimulator of archetype JCV replication. The expression of SV40 T antigen was enhanced by HIV‐1 Tat slightly according to real‐time RT‐PCR, this was not closely related to JCV replication in COS‐tat cells. The efficiency of JCV propagation depended on the extent of expression of functional Tat. To our knowledge, this is the first report of increased production of archetype JCV in a culture system using cell lines stably expressing HIV‐1 Tat. We propose here that COS‐tat cells are a useful tool for studying the role of Tat in archetype JCV replication in the development of PML.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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