Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France
Identifieur interne : 000132 ( Istex/Corpus ); précédent : 000131; suivant : 000133Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France
Auteurs : Florence Damond ; Cristian Apetrei ; David L. Robertson ; Sandrine Souquière ; Annie Leprêtre ; Sophie Matheron ; Jc Plantier ; Françoise Brun-Vézinet ; François SimonSource :
- Virology [ 0042-6822 ] ; 2001.
English descriptors
- KwdEn :
- African patients, Amino, Amino acid sequences, Amino acids, Amplification, Binding site, Bissau, Burkina faso, Cape verde, Cape verde islands, Cell counts, Charles nicolle, Cote lancet, Country codes, Damond, Different strains, Different subtypes, Disease control, Dual infection, Dual reactivity, Epidemiological differences, French cohort, French patients, Fresh pbmcs, Genetic diversity, Glycoprotein, Guinea bissau, Guinea bissau strains, Horizontal branches lengths, Human immunodeficiency virus type, Immunodeficiency, Immunodominant, Immunodominant domain, Immunodominant region, Ivory coast, Last extension step, Little loop, Loop peptides, Mali, Matheron, Mutation, Natural history, Nonhuman primates, Nucleotide sequences, Optical density, Peptide, Peptides mapping, Percent bootstraps, Phylogenetic, Phylogenetic analysis, Phylogenetic relationships, Phylogenetic trees, Primer, Primer pairs, Probable place, Putative recombinants, Room temperature, Screening assay, Screening tests, Senegal, Sequence alignment, Sequence database, Sequence names, Serological consequences, Significant differences, Simian immunodeficiency virus, Sivagm, Sivmnd, Sivmnd peptide, Sivsm, Sooty mangabeys, Subtype, Subtypes, Symptomatic patients, Transmembrane glycoprotein, Variability, Verde, Vertical separation, Viral, Viral diversity, Virol, Virological, Virological characteristics, Vivo pathogenicity, West africa, Western blot, Window length.
- Teeft :
- African patients, Amino, Amino acid sequences, Amino acids, Amplification, Binding site, Bissau, Burkina faso, Cape verde, Cape verde islands, Cell counts, Charles nicolle, Cote lancet, Country codes, Damond, Different strains, Different subtypes, Disease control, Dual infection, Dual reactivity, Epidemiological differences, French cohort, French patients, Fresh pbmcs, Genetic diversity, Glycoprotein, Guinea bissau, Guinea bissau strains, Horizontal branches lengths, Human immunodeficiency virus type, Immunodeficiency, Immunodominant, Immunodominant domain, Immunodominant region, Ivory coast, Last extension step, Little loop, Loop peptides, Mali, Matheron, Mutation, Natural history, Nonhuman primates, Nucleotide sequences, Optical density, Peptide, Peptides mapping, Percent bootstraps, Phylogenetic, Phylogenetic analysis, Phylogenetic relationships, Phylogenetic trees, Primer, Primer pairs, Probable place, Putative recombinants, Room temperature, Screening assay, Screening tests, Senegal, Sequence alignment, Sequence database, Sequence names, Serological consequences, Significant differences, Simian immunodeficiency virus, Sivagm, Sivmnd, Sivmnd peptide, Sivsm, Sooty mangabeys, Subtype, Subtypes, Symptomatic patients, Transmembrane glycoprotein, Variability, Verde, Vertical separation, Viral, Viral diversity, Virol, Virological, Virological characteristics, Vivo pathogenicity, West africa, Western blot, Window length.
Abstract
To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.
Url:
DOI: 10.1006/viro.2000.0685
Links to Exploration step
ISTEX:832CD343063A43D09131AD12BB07D0D4356CC606Le document en format XML
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density</term><term>Peptide</term><term>Peptides mapping</term><term>Percent bootstraps</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Phylogenetic relationships</term><term>Phylogenetic trees</term><term>Primer</term><term>Primer pairs</term><term>Probable place</term><term>Putative recombinants</term><term>Room temperature</term><term>Screening assay</term><term>Screening tests</term><term>Senegal</term><term>Sequence alignment</term><term>Sequence database</term><term>Sequence names</term><term>Serological consequences</term><term>Significant differences</term><term>Simian immunodeficiency virus</term><term>Sivagm</term><term>Sivmnd</term><term>Sivmnd peptide</term><term>Sivsm</term><term>Sooty mangabeys</term><term>Subtype</term><term>Subtypes</term><term>Symptomatic patients</term><term>Transmembrane glycoprotein</term><term>Variability</term><term>Verde</term><term>Vertical separation</term><term>Viral</term><term>Viral diversity</term><term>Virol</term><term>Virological</term><term>Virological characteristics</term><term>Vivo pathogenicity</term><term>West africa</term><term>Western blot</term><term>Window length</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>African patients</term><term>Amino</term><term>Amino acid sequences</term><term>Amino acids</term><term>Amplification</term><term>Binding site</term><term>Bissau</term><term>Burkina faso</term><term>Cape verde</term><term>Cape verde islands</term><term>Cell counts</term><term>Charles nicolle</term><term>Cote lancet</term><term>Country codes</term><term>Damond</term><term>Different strains</term><term>Different subtypes</term><term>Disease control</term><term>Dual infection</term><term>Dual reactivity</term><term>Epidemiological differences</term><term>French cohort</term><term>French patients</term><term>Fresh pbmcs</term><term>Genetic diversity</term><term>Glycoprotein</term><term>Guinea bissau</term><term>Guinea bissau strains</term><term>Horizontal branches lengths</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Immunodominant</term><term>Immunodominant domain</term><term>Immunodominant region</term><term>Ivory coast</term><term>Last extension step</term><term>Little loop</term><term>Loop peptides</term><term>Mali</term><term>Matheron</term><term>Mutation</term><term>Natural history</term><term>Nonhuman primates</term><term>Nucleotide sequences</term><term>Optical density</term><term>Peptide</term><term>Peptides mapping</term><term>Percent bootstraps</term><term>Phylogenetic</term><term>Phylogenetic analysis</term><term>Phylogenetic relationships</term><term>Phylogenetic trees</term><term>Primer</term><term>Primer pairs</term><term>Probable place</term><term>Putative recombinants</term><term>Room temperature</term><term>Screening assay</term><term>Screening tests</term><term>Senegal</term><term>Sequence alignment</term><term>Sequence database</term><term>Sequence names</term><term>Serological consequences</term><term>Significant differences</term><term>Simian immunodeficiency virus</term><term>Sivagm</term><term>Sivmnd</term><term>Sivmnd peptide</term><term>Sivsm</term><term>Sooty mangabeys</term><term>Subtype</term><term>Subtypes</term><term>Symptomatic patients</term><term>Transmembrane glycoprotein</term><term>Variability</term><term>Verde</term><term>Vertical separation</term><term>Viral</term><term>Viral diversity</term><term>Virol</term><term>Virological</term><term>Virological characteristics</term><term>Vivo pathogenicity</term><term>West africa</term><term>Western blot</term><term>Window length</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>phylogenetic</json:string><json:string>immunodeficiency</json:string><json:string>peptide</json:string><json:string>subtypes</json:string><json:string>subtype</json:string><json:string>ivory coast</json:string><json:string>verde</json:string><json:string>damond</json:string><json:string>sivagm</json:string><json:string>human immunodeficiency virus 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separation</json:string><json:string>percent bootstraps</json:string><json:string>sequence names</json:string><json:string>sooty mangabeys</json:string><json:string>probable place</json:string><json:string>putative recombinants</json:string><json:string>burkina faso</json:string><json:string>charles nicolle</json:string><json:string>serological consequences</json:string><json:string>peptides mapping</json:string><json:string>sivmnd peptide</json:string><json:string>french patients</json:string><json:string>amino acids</json:string><json:string>viral diversity</json:string><json:string>immunodominant domain</json:string><json:string>french cohort</json:string><json:string>african patients</json:string><json:string>disease control</json:string><json:string>fresh pbmcs</json:string><json:string>primer pairs</json:string><json:string>last extension step</json:string><json:string>natural history</json:string><json:string>window length</json:string><json:string>different strains</json:string><json:string>nonhuman primates</json:string><json:string>loop peptides</json:string><json:string>cote lancet</json:string><json:string>optical density</json:string><json:string>virological characteristics</json:string><json:string>sequence alignment</json:string><json:string>variability</json:string></teeft></keywords><author><json:item><name>Florence Damond</name><affiliations><json:string>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</json:string></affiliations></json:item><json:item><name>Cristian Apetrei</name><affiliations><json:string>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</json:string></affiliations></json:item><json:item><name>David L Robertson</name><affiliations><json:string>Information Génétique and Structurale, CNRS-UMR 1889, 13402, Marseille Cedex 20, France</json:string></affiliations></json:item><json:item><name>Sandrine Souquière</name><affiliations><json:string>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</json:string></affiliations></json:item><json:item><name>Annie Leprêtre</name><affiliations><json:string>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</json:string></affiliations></json:item><json:item><name>Sophie Matheron</name><affiliations><json:string>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</json:string></affiliations></json:item><json:item><name>JC Plantier</name><affiliations><json:string>Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031, Rouen, France</json:string></affiliations></json:item><json:item><name>Françoise Brun-Vézinet</name><affiliations><json:string>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</json:string></affiliations></json:item><json:item><name>François Simon</name><affiliations><json:string>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.</abstract><qualityIndicators><score>7.988</score><pdfVersion>1.3</pdfVersion><pdfPageSize>534 x 723 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1628</abstractCharCount><pdfWordCount>6368</pdfWordCount><pdfCharCount>39842</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>249</abstractWordCount></qualityIndicators><title>Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</title><pii><json:string>S0042-6822(00)90685-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>2001</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0025-6</json:string></pii><volume>280</volume><issue>1</issue><pages><first>19</first><last>30</last></pages><genre><json:string>journal</json:string></genre></host><categories><wos><json:string>science</json:string><json:string>virology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>virology</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string></inist></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1006/viro.2000.0685</json:string></doi><id>832CD343063A43D09131AD12BB07D0D4356CC606</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/832CD343063A43D09131AD12BB07D0D4356CC606/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/832CD343063A43D09131AD12BB07D0D4356CC606/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/832CD343063A43D09131AD12BB07D0D4356CC606/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Academic Press</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</title><author xml:id="author-0000"><persName><forename type="first">Florence</forename><surname>Damond</surname></persName><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Cristian</forename><surname>Apetrei</surname></persName><affiliation>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</affiliation></author><author xml:id="author-0002"><persName><forename type="first">David L</forename><surname>Robertson</surname></persName><affiliation>Information Génétique and Structurale, CNRS-UMR 1889, 13402, Marseille Cedex 20, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Sandrine</forename><surname>Souquière</surname></persName><affiliation>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Annie</forename><surname>Leprêtre</surname></persName><affiliation>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Sophie</forename><surname>Matheron</surname></persName><affiliation>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation></author><author xml:id="author-0006"><persName><forename type="first">JC</forename><surname>Plantier</surname></persName><affiliation>Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031, Rouen, France</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Françoise</forename><surname>Brun-Vézinet</surname></persName><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation></author><author xml:id="author-0008"><persName><forename type="first">François</forename><surname>Simon</surname></persName><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation></author><idno type="istex">832CD343063A43D09131AD12BB07D0D4356CC606</idno><idno type="DOI">10.1006/viro.2000.0685</idno><idno type="PII">S0042-6822(00)90685-8</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0025-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">280</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="19">19</biblScope><biblScope unit="page" to="30">30</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.</p></abstract></profileDesc><revisionDesc><change when="2000-09-25">Modified</change><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/832CD343063A43D09131AD12BB07D0D4356CC606/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>90685</aid><ce:pii>S0042-6822(00)90685-8</ce:pii><ce:doi>10.1006/viro.2000.0685</ce:doi><ce:copyright type="full-transfer" year="2001">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</ce:title><ce:author-group><ce:author><ce:given-name>Florence</ce:given-name><ce:surname>Damond</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Cristian</ce:given-name><ce:surname>Apetrei</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>David L</ce:given-name><ce:surname>Robertson</ce:surname><ce:cross-ref refid="A3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Sandrine</ce:given-name><ce:surname>Souquière</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Annie</ce:given-name><ce:surname>Leprêtre</ce:surname><ce:cross-ref refid="A4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Sophie</ce:given-name><ce:surname>Matheron</ce:surname><ce:cross-ref refid="A4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>JC</ce:given-name><ce:surname>Plantier</ce:surname><ce:cross-ref refid="A5"><ce:sup>e</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Françoise</ce:given-name><ce:surname>Brun-Vézinet</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>François</ce:given-name><ce:surname>Simon</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="A5"><ce:sup>e</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="A1"><ce:label>a</ce:label><ce:textfn>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</ce:textfn></ce:affiliation><ce:affiliation id="A4"><ce:label>d</ce:label><ce:textfn>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</ce:textfn></ce:affiliation><ce:affiliation id="A2"><ce:label>b</ce:label><ce:textfn>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</ce:textfn></ce:affiliation><ce:affiliation id="A3"><ce:label>c</ce:label><ce:textfn>Information Génétique and Structurale, CNRS-UMR 1889, 13402, Marseille Cedex 20, France</ce:textfn></ce:affiliation><ce:affiliation id="A5"><ce:label>e</ce:label><ce:textfn>Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031, Rouen, France</ce:textfn></ce:affiliation><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>To whom correspondence and reprint requests should be adressed at Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen, France. E-mail: francois.simon@chu-rouen.fr.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="21" month="6" year="2000"></ce:date-received><ce:date-revised day="25" month="9" year="2000"></ce:date-revised><ce:date-accepted day="3" month="10" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 <ce:italic>env</ce:italic> primers, and the <ce:italic>env</ce:italic> gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (<ce:italic>n</ce:italic> = 23) or B (<ce:italic>n</ce:italic> = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 <ce:italic>env</ce:italic> antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Variability of Human Immunodeficiency Virus Type 2 (HIV-2) Infecting Patients Living in France</title></titleInfo><name type="personal"><namePart type="given">Florence</namePart><namePart type="family">Damond</namePart><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Cristian</namePart><namePart type="family">Apetrei</namePart><affiliation>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David L</namePart><namePart type="family">Robertson</namePart><affiliation>Information Génétique and Structurale, CNRS-UMR 1889, 13402, Marseille Cedex 20, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sandrine</namePart><namePart type="family">Souquière</namePart><affiliation>Laboratoire de Virologie, Centre International de Recherches Médicales de Franceville, BP 769, Franceville, Gabon</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Annie</namePart><namePart type="family">Leprêtre</namePart><affiliation>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sophie</namePart><namePart type="family">Matheron</namePart><affiliation>Service des Maladies Infectieuses et Tropicales, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JC</namePart><namePart type="family">Plantier</namePart><affiliation>Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031, Rouen, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">Brun-Vézinet</namePart><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">François</namePart><namePart type="family">Simon</namePart><affiliation>Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 75877, Paris Cedex 18, France</affiliation><description>To whom correspondence and reprint requests should be adressed at Laboratoire de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen, France. E-mail: francois.simon@chu-rouen.fr.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><dateModified encoding="w3cdtf">2000-09-25</dateModified><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">To determine the prevalence of human immunodeficiency virus type 2 (HIV-2) subtypes circulating in France and to identify possible relationships between these subtypes and pathogenesis, we studied 33 HIV-2-infected patients living in France. HIV-2 DNA was directly amplified from peripheral blood mononuclear cells by nested PCR with specific HIV-2 env primers, and the env gene was sequenced. The serological consequences of antigenic variability were studied by using a panel of peptides and by Western blotting. Phylogenetic analysis classified the 33 HIV-2 strains as subtype A (n = 23) or B (n = 10). There were no significant clinical or epidemiological differences between patients infected with either of these two subtypes. There was some evidence for geographical clustering. Subtype A strains from patients originating from the Cape Verde Islands and Guinea Bissau clustered together. The majority of patients infected with subtype B strains originated from the Ivory Coast or Mali. Strains from patients originating in Mali also clustered in subtype A but distinctly from the Cape Verde or Guinea Bissau strains. The subtype B strains showed greater diversity and included some highly divergent strains relative to those previously characterized. The V3 loop of HIV-2 subtypes A and B was found to be quite conserved in comparison with HIV-1. A strong HIV-2 subtype B serological cross-reactivity was found on HIV-1 env antigen by Western blot mostly in the gp41 transmembrane glycoprotein. This could partly explain the double HIV-1 and HIV-2 reactive profiles found in countries where HIV-2 subtype B is prevalent.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20010201</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0025-6</identifier><part><date>20010201</date><detail type="volume"><number>280</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>153</end></extent><extent unit="pages"><start>19</start><end>30</end></extent></part></relatedItem><identifier type="istex">832CD343063A43D09131AD12BB07D0D4356CC606</identifier><identifier type="DOI">10.1006/viro.2000.0685</identifier><identifier type="PII">S0042-6822(00)90685-8</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Academic Press</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Academic Press, ©2001</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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