Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.
Identifieur interne : 000248 ( Main/Corpus ); précédent : 000247; suivant : 000249Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis.
Auteurs : W. Omata ; H. Shibata ; Y. Suzuki ; S. Tanaka ; T. Suzuki ; K. Takata ; I. KojimaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1997.
English descriptors
- KwdEn :
- Animals, Biological Transport, CHO Cells, Cell Compartmentation, Cricetinae, Dynamins, Endocytosis, GTP Phosphohydrolases (genetics), GTP Phosphohydrolases (metabolism), Glucose Transporter Type 4, Monosaccharide Transport Proteins (isolation & purification), Monosaccharide Transport Proteins (metabolism), Muscle Proteins, Peptide Fragments (genetics), Peptide Fragments (metabolism), Receptor, Insulin (metabolism), Recombinant Proteins (metabolism).
- MESH :
- chemical , genetics : GTP Phosphohydrolases, Peptide Fragments.
- chemical , isolation & purification : Monosaccharide Transport Proteins.
- chemical , metabolism : GTP Phosphohydrolases, Monosaccharide Transport Proteins, Peptide Fragments, Receptor, Insulin, Recombinant Proteins.
- chemical : Dynamins, Glucose Transporter Type 4, Muscle Proteins.
- Animals, Biological Transport, CHO Cells, Cell Compartmentation, Cricetinae, Endocytosis.
Abstract
We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.
DOI: 10.1006/bbrc.1997.7810
PubMed: 9425283
Links to Exploration step
pubmed:9425283Le document en format XML
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<author><name sortKey="Omata, W" sort="Omata, W" uniqKey="Omata W" first="W" last="Omata">W. Omata</name>
<affiliation><nlm:affiliation>Department of Cell Biology, Gunma University, Maebashi, Japan.</nlm:affiliation>
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<author><name sortKey="Shibata, H" sort="Shibata, H" uniqKey="Shibata H" first="H" last="Shibata">H. Shibata</name>
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<author><name sortKey="Suzuki, Y" sort="Suzuki, Y" uniqKey="Suzuki Y" first="Y" last="Suzuki">Y. Suzuki</name>
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<author><name sortKey="Tanaka, S" sort="Tanaka, S" uniqKey="Tanaka S" first="S" last="Tanaka">S. Tanaka</name>
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<author><name sortKey="Suzuki, T" sort="Suzuki, T" uniqKey="Suzuki T" first="T" last="Suzuki">T. Suzuki</name>
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<author><name sortKey="Takata, K" sort="Takata, K" uniqKey="Takata K" first="K" last="Takata">K. Takata</name>
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<author><name sortKey="Kojima, I" sort="Kojima, I" uniqKey="Kojima I" first="I" last="Kojima">I. Kojima</name>
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<author><name sortKey="Suzuki, Y" sort="Suzuki, Y" uniqKey="Suzuki Y" first="Y" last="Suzuki">Y. Suzuki</name>
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<author><name sortKey="Takata, K" sort="Takata, K" uniqKey="Takata K" first="K" last="Takata">K. Takata</name>
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<term>Cell Compartmentation</term>
<term>Cricetinae</term>
<term>Dynamins</term>
<term>Endocytosis</term>
<term>GTP Phosphohydrolases (genetics)</term>
<term>GTP Phosphohydrolases (metabolism)</term>
<term>Glucose Transporter Type 4</term>
<term>Monosaccharide Transport Proteins (isolation & purification)</term>
<term>Monosaccharide Transport Proteins (metabolism)</term>
<term>Muscle Proteins</term>
<term>Peptide Fragments (genetics)</term>
<term>Peptide Fragments (metabolism)</term>
<term>Receptor, Insulin (metabolism)</term>
<term>Recombinant Proteins (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>GTP Phosphohydrolases</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Monosaccharide Transport Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>GTP Phosphohydrolases</term>
<term>Monosaccharide Transport Proteins</term>
<term>Peptide Fragments</term>
<term>Receptor, Insulin</term>
<term>Recombinant Proteins</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dynamins</term>
<term>Glucose Transporter Type 4</term>
<term>Muscle Proteins</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Biological Transport</term>
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<front><div type="abstract" xml:lang="en">We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.</div>
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<Abstract><AbstractText>We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHOIR-GLUT4-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHOIR-GLUT4-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHOIR-GLUT4-WT cells but had little effect in CHOIR-GLUT4-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHOIR-GLUT4-WT cell, whereas it remained at the cell surface in CHOIR-GLUT4-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.</AbstractText>
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