Co-grafts of muscle cells and mesencephalic tissue into hemiparkinsonian rats: behavioral and histochemical effects
Identifieur interne : 001C52 ( Main/Exploration ); précédent : 001C51; suivant : 001C53Co-grafts of muscle cells and mesencephalic tissue into hemiparkinsonian rats: behavioral and histochemical effects
Auteurs : F. G Kaddis [États-Unis] ; E. D Clarkson [États-Unis] ; K. P Bell [États-Unis] ; P. K Choi [États-Unis] ; C. R Freed [États-Unis]Source :
- Brain Research Bulletin [ 0361-9230 ] ; 1999.
English descriptors
- KwdEn :
Abstract
Extracts from skeletal muscle cell cultures have been shown to increase levels of the enzyme tyrosine hydroxylase (TH) and promote survival of different types of developing neurons in vitro. To determine the effect of muscle cell co-grafts on the survival of dopamine neurons in a rat model of Parkinson’s disease, we transplanted an embryonic day (ED)-15 rat mesencephalic cell suspension alone or with neonatal muscle cells into 6-hydroxydopamine (6-OHDA) denervated rat striatum. In parallel experiments conducted in vitro, we cultured ED-15 rat mesencephalon or rat striatum in conditioned medium from neonatal rat muscle cultures (MC-CM). Our results showed that: (A) in vitro, MC-CM increased the number of TH-immunoreactive (TH-IR) neurons in embryonic mesencephalic cultures but did not induce expression of TH in embryonic striatal cultures; (B) in vivo, animals with co-grafts of muscle cells and ED-15 mesencephalon had more TH-IR in the grafted striatum compared to animals that received mesencephalic cells grafts alone, although the graft-induced reversal of circling behavior in response to methamphetamine was the same in both transplanted groups; and (C) grafts of muscle cells alone did not induce TH-IR in the denervated striatum and did not reduce methamphetamine-induced circling. These findings suggest that in vivo, neonatal muscle cells secrete factors that promote survival and/or outgrowth of fetal midbrain dopamine cells and improve the levels of TH-IR in grafted striatum.
Url:
DOI: 10.1016/S0361-9230(99)00216-6
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Co-grafts of muscle cells and mesencephalic tissue into hemiparkinsonian rats: behavioral and histochemical effects</title><author><name sortKey="Kaddis, F G" sort="Kaddis, F G" uniqKey="Kaddis F" first="F. G" last="Kaddis">F. G Kaddis</name></author><author><name sortKey="Clarkson, E D" sort="Clarkson, E D" uniqKey="Clarkson E" first="E. D" last="Clarkson">E. D Clarkson</name></author><author><name sortKey="Bell, K P" sort="Bell, K P" uniqKey="Bell K" first="K. P" last="Bell">K. P Bell</name></author><author><name sortKey="Choi, P K" sort="Choi, P K" uniqKey="Choi P" first="P. K" last="Choi">P. K Choi</name></author><author><name sortKey="Freed, C R" sort="Freed, C R" uniqKey="Freed C" first="C. R" last="Freed">C. R Freed</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:88A5265151EDD5E189B21F0ACEC767298A711038</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0361-9230(99)00216-6</idno><idno type="url">https://api.istex.fr/document/88A5265151EDD5E189B21F0ACEC767298A711038/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002037</idno><idno type="wicri:Area/Main/Curation">001D38</idno><idno type="wicri:Area/Main/Exploration">001C52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Co-grafts of muscle cells and mesencephalic tissue into hemiparkinsonian rats: behavioral and histochemical effects</title><author><name sortKey="Kaddis, F G" sort="Kaddis, F G" uniqKey="Kaddis F" first="F. G" last="Kaddis">F. G Kaddis</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Clarkson, E D" sort="Clarkson, E D" uniqKey="Clarkson E" first="E. D" last="Clarkson">E. D Clarkson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Bell, K P" sort="Bell, K P" uniqKey="Bell K" first="K. P" last="Bell">K. P Bell</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Choi, P K" sort="Choi, P K" uniqKey="Choi P" first="P. K" last="Choi">P. K Choi</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation></author><author><name sortKey="Freed, C R" sort="Freed, C R" uniqKey="Freed C" first="C. R" last="Freed">C. R Freed</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver, CO</wicri:regionArea><placeName><region type="state">Colorado</region></placeName></affiliation><affiliation><wicri:noCountry code="subField">272</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research Bulletin</title><title level="j" type="abbrev">BRB</title><idno type="ISSN">0361-9230</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">51</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="203">203</biblScope><biblScope unit="page" to="211">211</biblScope></imprint><idno type="ISSN">0361-9230</idno></series><idno type="istex">88A5265151EDD5E189B21F0ACEC767298A711038</idno><idno type="DOI">10.1016/S0361-9230(99)00216-6</idno><idno type="PII">S0361-9230(99)00216-6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0361-9230</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Parkinson’s disease</term><term>conditioned medium</term><term>differentiation</term><term>dopamine</term><term>muscle cells</term><term>neurotransplantation</term><term>trophic factors</term><term>tyrosine hydroxylase</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Extracts from skeletal muscle cell cultures have been shown to increase levels of the enzyme tyrosine hydroxylase (TH) and promote survival of different types of developing neurons in vitro. To determine the effect of muscle cell co-grafts on the survival of dopamine neurons in a rat model of Parkinson’s disease, we transplanted an embryonic day (ED)-15 rat mesencephalic cell suspension alone or with neonatal muscle cells into 6-hydroxydopamine (6-OHDA) denervated rat striatum. In parallel experiments conducted in vitro, we cultured ED-15 rat mesencephalon or rat striatum in conditioned medium from neonatal rat muscle cultures (MC-CM). Our results showed that: (A) in vitro, MC-CM increased the number of TH-immunoreactive (TH-IR) neurons in embryonic mesencephalic cultures but did not induce expression of TH in embryonic striatal cultures; (B) in vivo, animals with co-grafts of muscle cells and ED-15 mesencephalon had more TH-IR in the grafted striatum compared to animals that received mesencephalic cells grafts alone, although the graft-induced reversal of circling behavior in response to methamphetamine was the same in both transplanted groups; and (C) grafts of muscle cells alone did not induce TH-IR in the denervated striatum and did not reduce methamphetamine-induced circling. These findings suggest that in vivo, neonatal muscle cells secrete factors that promote survival and/or outgrowth of fetal midbrain dopamine cells and improve the levels of TH-IR in grafted striatum.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Colorado</li></region></list><tree><country name="États-Unis"><region name="Colorado"><name sortKey="Kaddis, F G" sort="Kaddis, F G" uniqKey="Kaddis F" first="F. G" last="Kaddis">F. G Kaddis</name></region><name sortKey="Bell, K P" sort="Bell, K P" uniqKey="Bell K" first="K. P" last="Bell">K. P Bell</name><name sortKey="Choi, P K" sort="Choi, P K" uniqKey="Choi P" first="P. K" last="Choi">P. K Choi</name><name sortKey="Clarkson, E D" sort="Clarkson, E D" uniqKey="Clarkson E" first="E. D" last="Clarkson">E. D Clarkson</name><name sortKey="Freed, C R" sort="Freed, C R" uniqKey="Freed C" first="C. R" last="Freed">C. R Freed</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C52 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001C52 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:88A5265151EDD5E189B21F0ACEC767298A711038
|texte= Co-grafts of muscle cells and mesencephalic tissue into hemiparkinsonian rats: behavioral and histochemical effects
}}
| This area was generated with Dilib version V0.6.23. |  |