Cytoplasmic Transfer of Platelet mtDNA from Elderly Patients with Parkinson's Disease to mtDNA-less HeLa Cells Restores Complete Mitochondrial Respiratory Function
Identifieur interne : 001A88 ( Main/Exploration ); précédent : 001A87; suivant : 001A89Cytoplasmic Transfer of Platelet mtDNA from Elderly Patients with Parkinson's Disease to mtDNA-less HeLa Cells Restores Complete Mitochondrial Respiratory Function
Auteurs : Yuko Aomi [Japon] ; Chu-Shih Chen [Japon] ; Kazuto Nakada [Japon] ; Sayaka Ito [Japon] ; Kotoyo Isobe [Japon] ; Haruka Murakami [Japon] ; Shin-Ya Kuno [Japon] ; Masato Tawata [Japon] ; Rumiko Matsuoka [Japon] ; Hidehiro Mizusawa [Japon] ; Jun-Ichi Hayashi [Japon]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2000.
Abstract
For determination of whether platelet mtDNA in patients with Parkinson's disease (PD) possesses some lesions to reduce respiratory enzyme activities, platelet mtDNA was transferred into mtDNA-less (ρ0) HeLa cells from aged PD patients and age-matched normal subjects, since their activities were controlled by both mitochondrial and nuclear genomes. The resultant mtDNA-repopulated cybrid clones containing the HeLa nuclear genome as a common background were used for comparison of respiratory enzyme activities. Remarkable variations of the enzyme activities were observed in the cybrid clones, irrespective of whether their mtDNA was transferred from normal subjects or PD patients, and some of them showed 20% reduction of average activities. Thus, the mtDNA mutations responsible for inducing 20% reduction should be polymorphic rather than pathogenic. On the other hand, pathogenic control cybrid clones possessing mtDNA mutations from patients with mitochondrial disorders showed significant and specific decline of respiratory enzyme complex I activity beyond the normal range of the variations. These observations warrant reassessment of the conventional concept that complex I activity in platelets of PD patients is defective due to mtDNA mutations.
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DOI: 10.1006/bbrc.2000.4113
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possesses some lesions to reduce respiratory enzyme activities, platelet mtDNA was transferred into mtDNA-less (ρ0) HeLa cells from aged PD patients and age-matched normal subjects, since their activities were controlled by both mitochondrial and nuclear genomes. The resultant mtDNA-repopulated cybrid clones containing the HeLa nuclear genome as a common background were used for comparison of respiratory enzyme activities. Remarkable variations of the enzyme activities were observed in the cybrid clones, irrespective of whether their mtDNA was transferred from normal subjects or PD patients, and some of them showed 20% reduction of average activities. Thus, the mtDNA mutations responsible for inducing 20% reduction should be polymorphic rather than pathogenic. On the other hand, pathogenic control cybrid clones possessing mtDNA mutations from patients with mitochondrial disorders showed significant and specific decline of respiratory enzyme complex I activity beyond the normal range of the variations. These observations warrant reassessment of the conventional concept that complex I activity in platelets of PD patients is defective due to mtDNA mutations.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Aomi, Yuko" sort="Aomi, Yuko" uniqKey="Aomi Y" first="Yuko" last="Aomi">Yuko Aomi</name></noRegion><name sortKey="Chen, Chu Shih" sort="Chen, Chu Shih" uniqKey="Chen C" first="Chu-Shih" last="Chen">Chu-Shih Chen</name><name sortKey="Hayashi, Jun Ichi" sort="Hayashi, Jun Ichi" uniqKey="Hayashi J" first="Jun-Ichi" last="Hayashi">Jun-Ichi Hayashi</name><name sortKey="Isobe, Kotoyo" sort="Isobe, Kotoyo" uniqKey="Isobe K" first="Kotoyo" last="Isobe">Kotoyo Isobe</name><name sortKey="Ito, Sayaka" sort="Ito, Sayaka" uniqKey="Ito S" first="Sayaka" last="Ito">Sayaka Ito</name><name sortKey="Kuno, Shin Ya" sort="Kuno, Shin Ya" uniqKey="Kuno S" first="Shin-Ya" last="Kuno">Shin-Ya Kuno</name><name sortKey="Matsuoka, Rumiko" sort="Matsuoka, Rumiko" uniqKey="Matsuoka R" first="Rumiko" last="Matsuoka">Rumiko Matsuoka</name><name sortKey="Mizusawa, Hidehiro" sort="Mizusawa, Hidehiro" uniqKey="Mizusawa H" first="Hidehiro" last="Mizusawa">Hidehiro Mizusawa</name><name sortKey="Murakami, Haruka" sort="Murakami, Haruka" uniqKey="Murakami H" first="Haruka" last="Murakami">Haruka Murakami</name><name sortKey="Nakada, Kazuto" sort="Nakada, Kazuto" uniqKey="Nakada K" first="Kazuto" last="Nakada">Kazuto Nakada</name><name sortKey="Tawata, Masato" sort="Tawata, Masato" uniqKey="Tawata M" first="Masato" last="Tawata">Masato Tawata</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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