Allelic imbalance of expression and epigenetic regulation within the alpha‐synuclein wild‐type and p.Ala53Thr alleles in Parkinson disease
Identifieur interne : 000535 ( Main/Curation ); précédent : 000534; suivant : 000536Allelic imbalance of expression and epigenetic regulation within the alpha‐synuclein wild‐type and p.Ala53Thr alleles in Parkinson disease
Auteurs : Gerassimos E. Voutsinas [Grèce] ; Eleana F. Stavrou [Grèce] ; Gerassimos Karousos [Grèce] ; Aggeliki Dasoula [Grèce] ; Adamantia Papachatzopoulou [Grèce] ; Maria Syrrou [Grèce] ; Annemieke J. M. H. Verkerk [Pays-Bas] ; Peter Van Der Spek [Pays-Bas] ; George P. Patrinos [Pays-Bas, Grèce] ; Reinhard Stöger [États-Unis, Royaume-Uni] ; Aglaia Athanassiadou [Grèce]Source :
- Human Mutation [ 1059-7794 ] ; 2010-06.
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Abstract
Genetic alterations in the alpha‐synuclein (SNCA) gene have been implicated in Parkinson Disease (PD), including point mutations, gene multiplications, and sequence variations within the promoter. Such alterations may be involved in pathology through structural changes or overexpression of the protein leading to protein aggregation, as well as through impaired gene expression. It is, therefore, of importance to specify the parameters that regulate SNCA expression in its normal and mutated state. We studied the expression of SNCA alleles in a lymphoblastoid cell line and in the blood cells of a patient heterozygous for p.Ala53Thr, the first mutation to be implicated in PD pathogenesis. Here, we provide evidence that: (1) SNCA shows monoallelic expression in this patient, (2) epigenetic silencing of the mutated allele involves histone modifications but not DNA methylation, and (3) steady‐state mRNA levels deriving from the normal SNCA allele in this patient exceed those of the two normal SNCA alleles combined, in matching, control individuals. An imbalanced SNCA expression in this patient is thus documented, with silencing of the p.Ala53Thr allele and upregulation of the wild‐type‐allele. This phenomenon is demonstrated for a first time in the SNCA gene, and may have important implications for PD pathogenesis. Hum Mutat 31:1–7, 2010. © 2010 Wiley‐Liss, Inc.
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DOI: 10.1002/humu.21248
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M. H." last="Verkerk">Annemieke J. M. H. Verkerk</name><affiliation wicri:level="1"><mods:affiliation>Erasmus University Medical Center, Faculty of Medicine and Health Sciences, Department of Bioinformatics, Rotterdam, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Erasmus University Medical Center, Faculty of Medicine and Health Sciences, Department of Bioinformatics, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Van Der Spek, Peter" sort="Van Der Spek, Peter" uniqKey="Van Der Spek P" first="Peter" last="Van Der Spek">Peter Van Der Spek</name><affiliation wicri:level="1"><mods:affiliation>Erasmus University Medical Center, Faculty of Medicine and Health Sciences, Department of Bioinformatics, Rotterdam, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Erasmus University Medical Center, Faculty of Medicine and Health Sciences, Department of Bioinformatics, Rotterdam</wicri:regionArea></affiliation></author><author><name sortKey="Patrinos, George P" sort="Patrinos, George P" uniqKey="Patrinos G" first="George P." last="Patrinos">George P. 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Mutat.</title><idno type="ISSN">1059-7794</idno><idno type="eISSN">1098-1004</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2010-06">2010-06</date><biblScope unit="volume">31</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="685">685</biblScope><biblScope unit="page" to="691">691</biblScope></imprint><idno type="ISSN">1059-7794</idno></series><idno type="istex">0780772AEDD857BDB4A67C3DC98CFF472926E7B0</idno><idno type="DOI">10.1002/humu.21248</idno><idno type="ArticleID">HUMU21248</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1059-7794</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Parkinson</term><term>SNCA</term><term>alpha‐synuclein</term><term>epigenetic</term><term>monoallelic</term><term>overexpression</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic alterations in the alpha‐synuclein (SNCA) gene have been implicated in Parkinson Disease (PD), including point mutations, gene multiplications, and sequence variations within the promoter. Such alterations may be involved in pathology through structural changes or overexpression of the protein leading to protein aggregation, as well as through impaired gene expression. It is, therefore, of importance to specify the parameters that regulate SNCA expression in its normal and mutated state. We studied the expression of SNCA alleles in a lymphoblastoid cell line and in the blood cells of a patient heterozygous for p.Ala53Thr, the first mutation to be implicated in PD pathogenesis. Here, we provide evidence that: (1) SNCA shows monoallelic expression in this patient, (2) epigenetic silencing of the mutated allele involves histone modifications but not DNA methylation, and (3) steady‐state mRNA levels deriving from the normal SNCA allele in this patient exceed those of the two normal SNCA alleles combined, in matching, control individuals. An imbalanced SNCA expression in this patient is thus documented, with silencing of the p.Ala53Thr allele and upregulation of the wild‐type‐allele. This phenomenon is demonstrated for a first time in the SNCA gene, and may have important implications for PD pathogenesis. Hum Mutat 31:1–7, 2010. © 2010 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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