One round of SELEX for the generation of DNA aptamers directed against KLK6
Identifieur interne : 002D84 ( Main/Corpus ); précédent : 002D83; suivant : 002D85One round of SELEX for the generation of DNA aptamers directed against KLK6
Auteurs : Steve Arnold ; Georgios Pampalakis ; Kalliopi Kantiotou ; Dilson Silva ; Celia Cortez ; Sotiris Missailidis ; Georgia SotiropoulouSource :
- Biological Chemistry [ 1431-6730 ] ; 2012-05-01.
Abstract
Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (Kd in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.
Url:
DOI: 10.1515/hsz-2011-0253
Links to Exploration step
ISTEX:131CB1A08EF156270B1029CA47DBCF40E16085E0Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">One round of SELEX for the generation of DNA aptamers directed against KLK6</title><author><name sortKey="Arnold, Steve" sort="Arnold, Steve" uniqKey="Arnold S" first="Steve" last="Arnold">Steve Arnold</name><affiliation><mods:affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Pampalakis, Georgios" sort="Pampalakis, Georgios" uniqKey="Pampalakis G" first="Georgios" last="Pampalakis">Georgios Pampalakis</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author><author><name sortKey="Kantiotou, Kalliopi" sort="Kantiotou, Kalliopi" uniqKey="Kantiotou K" first="Kalliopi" last="Kantiotou">Kalliopi Kantiotou</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author><author><name sortKey="Silva, Dilson" sort="Silva, Dilson" uniqKey="Silva D" first="Dilson" last="Silva">Dilson Silva</name><affiliation><mods:affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Cortez, Celia" sort="Cortez, Celia" uniqKey="Cortez C" first="Celia" last="Cortez">Celia Cortez</name><affiliation><mods:affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Missailidis, Sotiris" sort="Missailidis, Sotiris" uniqKey="Missailidis S" first="Sotiris" last="Missailidis">Sotiris Missailidis</name><affiliation><mods:affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Sotiropoulou, Georgia" sort="Sotiropoulou, Georgia" uniqKey="Sotiropoulou G" first="Georgia" last="Sotiropoulou">Georgia Sotiropoulou</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:131CB1A08EF156270B1029CA47DBCF40E16085E0</idno><date when="2012" year="2012">2012</date><idno type="doi">10.1515/hsz-2011-0253</idno><idno type="url">https://api.istex.fr/document/131CB1A08EF156270B1029CA47DBCF40E16085E0/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002D84</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">One round of SELEX for the generation of DNA aptamers directed against KLK6</title><author><name sortKey="Arnold, Steve" sort="Arnold, Steve" uniqKey="Arnold S" first="Steve" last="Arnold">Steve Arnold</name><affiliation><mods:affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Pampalakis, Georgios" sort="Pampalakis, Georgios" uniqKey="Pampalakis G" first="Georgios" last="Pampalakis">Georgios Pampalakis</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author><author><name sortKey="Kantiotou, Kalliopi" sort="Kantiotou, Kalliopi" uniqKey="Kantiotou K" first="Kalliopi" last="Kantiotou">Kalliopi Kantiotou</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author><author><name sortKey="Silva, Dilson" sort="Silva, Dilson" uniqKey="Silva D" first="Dilson" last="Silva">Dilson Silva</name><affiliation><mods:affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Cortez, Celia" sort="Cortez, Celia" uniqKey="Cortez C" first="Celia" last="Cortez">Celia Cortez</name><affiliation><mods:affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Missailidis, Sotiris" sort="Missailidis, Sotiris" uniqKey="Missailidis S" first="Sotiris" last="Missailidis">Sotiris Missailidis</name><affiliation><mods:affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</mods:affiliation></affiliation></author><author><name sortKey="Sotiropoulou, Georgia" sort="Sotiropoulou, Georgia" uniqKey="Sotiropoulou G" first="Georgia" last="Sotiropoulou">Georgia Sotiropoulou</name><affiliation><mods:affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Biological Chemistry</title><idno type="ISSN">1431-6730</idno><idno type="eISSN">1437-4315</idno><imprint><publisher>Walter de Gruyter</publisher><date type="published" when="2012-05-01">2012-05-01</date><biblScope unit="volume">393</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="343">343</biblScope><biblScope unit="page" to="353">353</biblScope></imprint><idno type="ISSN">1431-6730</idno></series><idno type="istex">131CB1A08EF156270B1029CA47DBCF40E16085E0</idno><idno type="DOI">10.1515/hsz-2011-0253</idno><idno type="ArticleID">hsz-2011-0253</idno><idno type="Related-article-Href">hsz-2011-0253.pdf</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1431-6730</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (Kd in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.</div></front></TEI><istex><corpusName>degruyter-journals</corpusName><author><json:item><name>Steve Arnold</name><affiliations><json:string>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</json:string></affiliations></json:item><json:item><name>Georgios Pampalakis</name><affiliations><json:string>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</json:string></affiliations></json:item><json:item><name>Kalliopi Kantiotou</name><affiliations><json:string>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</json:string></affiliations></json:item><json:item><name>Dilson Silva</name><affiliations><json:string>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</json:string></affiliations></json:item><json:item><name>Celia Cortez</name><affiliations><json:string>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</json:string></affiliations></json:item><json:item><name>Sotiris Missailidis</name><affiliations><json:string>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</json:string></affiliations></json:item><json:item><name>Georgia Sotiropoulou</name><affiliations><json:string>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>DNA aptamers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>kallikrein-related peptidase 6</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SELEX</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (Kd in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.</abstract><qualityIndicators><score>8.304</score><pdfVersion>1.5</pdfVersion><pdfPageSize>595.276 x 793.701 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>3</keywordCount><abstractCharCount>1362</abstractCharCount><pdfWordCount>7003</pdfWordCount><pdfCharCount>43512</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>192</abstractWordCount></qualityIndicators><title>One round of SELEX for the generation of DNA aptamers directed against KLK6</title><genre><json:string>research-article</json:string></genre><host><volume>393</volume><pages><last>353</last><first>343</first></pages><issn><json:string>1431-6730</json:string></issn><issue>5</issue><genre></genre><language><json:string>unknown</json:string></language><eissn><json:string>1437-4315</json:string></eissn><title>Biological Chemistry</title></host><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1515/hsz-2011-0253</json:string></doi><id>131CB1A08EF156270B1029CA47DBCF40E16085E0</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/131CB1A08EF156270B1029CA47DBCF40E16085E0/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/131CB1A08EF156270B1029CA47DBCF40E16085E0/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/131CB1A08EF156270B1029CA47DBCF40E16085E0/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">One round of SELEX for the generation of DNA aptamers directed against KLK6</title><respStmt xml:id="ISTEX-API" resp="Références bibliographiques récupérées via GROBID" name="ISTEX-API (INIST-CNRS)"></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Walter de Gruyter</publisher><availability><p>De Gruyter</p></availability><date>2012</date></publicationStmt><notesStmt><note>Corresponding authors</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">One round of SELEX for the generation of DNA aptamers directed against KLK6</title><author><persName><forename type="first">Steve</forename><surname>Arnold</surname></persName><affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</affiliation></author><author><persName><forename type="first">Georgios</forename><surname>Pampalakis</surname></persName><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation></author><author><persName><forename type="first">Kalliopi</forename><surname>Kantiotou</surname></persName><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation></author><author><persName><forename type="first">Dilson</forename><surname>Silva</surname></persName><affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</affiliation></author><author><persName><forename type="first">Celia</forename><surname>Cortez</surname></persName><affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</affiliation></author><author><persName><forename type="first">Sotiris</forename><surname>Missailidis</surname></persName><affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</affiliation></author><author><persName><forename type="first">Georgia</forename><surname>Sotiropoulou</surname></persName><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation></author></analytic><monogr><title level="j">Biological Chemistry</title><idno type="pISSN">1431-6730</idno><idno type="eISSN">1437-4315</idno><imprint><publisher>Walter de Gruyter</publisher><date type="published" when="2012-05-01"></date><biblScope unit="volume">393</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="343">343</biblScope><biblScope unit="page" to="353">353</biblScope></imprint></monogr><idno type="istex">131CB1A08EF156270B1029CA47DBCF40E16085E0</idno><idno type="DOI">10.1515/hsz-2011-0253</idno><idno type="ArticleID">hsz-2011-0253</idno><idno type="Related-article-Href">hsz-2011-0253.pdf</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (Kd in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>DNA aptamers</term></item><item><term>kallikrein-related peptidase 6</term></item><item><term>SELEX</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-05-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-3-13">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/131CB1A08EF156270B1029CA47DBCF40E16085E0/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus degruyter-journals" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Atypon//DTD Atypon Systems Journal Archiving and Interchange NLM DTD v3.0.2 20101108//EN" URI="atypon_archive-interchange-dtd-3.0.2/atypon-archivearticle3.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article" xml:lang="EN"><front><journal-meta><journal-id journal-id-type="publisher-id">bchm</journal-id><journal-title-group><abbrev-journal-title abbrev-type="full">Biological Chemistry</abbrev-journal-title></journal-title-group><issn pub-type="epub">1437-4315</issn><issn pub-type="ppub">1431-6730</issn><publisher><publisher-name>Walter de Gruyter</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">hsz-2011-0253</article-id><article-id pub-id-type="doi">10.1515/hsz-2011-0253</article-id><article-categories><subj-group subj-group-type="heading"><subject></subject></subj-group></article-categories><title-group><article-title>One round of SELEX for the generation of DNA aptamers directed against KLK6</article-title></title-group><contrib-group><contrib contrib-type="author"><name name-style="western"><surname>Arnold</surname><given-names>Steve</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Pampalakis</surname><given-names>Georgios</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Kantiotou</surname><given-names>Kalliopi</given-names></name><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Silva</surname><given-names>Dilson</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Cortez</surname><given-names>Celia</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Missailidis</surname><given-names>Sotiris</given-names></name><email>gdsotiro@upatras.gr</email><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Sotiropoulou</surname><given-names>Georgia</given-names></name><email>s.missailidis@open.ac.uk</email><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><aff id="aff1"><sup>1</sup>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</aff><aff id="aff2"><sup>2</sup>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</aff><aff id="aff3"><sup>3</sup>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</aff></contrib-group><author-notes><corresp>Corresponding authors</corresp></author-notes><pub-date pub-type="ppub"><day>01</day><month>05</month><year>2012</year><string-date>May 2012</string-date></pub-date><volume>393</volume><issue>5</issue><fpage>343</fpage><lpage>353</lpage><history><date date-type="received"><day>8</day><month>11</month><year>2011</year></date><date date-type="accepted"><day>31</day><month>1</month><year>2012</year></date></history><permissions><copyright-statement>©2012 by Walter de Gruyter Berlin Boston</copyright-statement><copyright-year>2012</copyright-year></permissions><related-article related-article-type="pdf" xlink:href="hsz-2011-0253.pdf"></related-article><abstract><title>Abstract</title><p>Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (<italic>K</italic><sub>d</sub> in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.</p></abstract><kwd-group><title>Keywords</title><kwd>DNA aptamers</kwd><kwd>kallikrein-related peptidase 6</kwd><kwd>SELEX</kwd></kwd-group><counts><fig-count count="6"></fig-count><table-count count="0"></table-count><ref-count count="44"></ref-count></counts></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>One round of SELEX for the generation of DNA aptamers directed against KLK6</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>One round of SELEX for the generation of DNA aptamers directed against KLK6</title></titleInfo><name type="personal"><namePart type="given">Steve</namePart><namePart type="family">Arnold</namePart><affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Georgios</namePart><namePart type="family">Pampalakis</namePart><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kalliopi</namePart><namePart type="family">Kantiotou</namePart><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dilson</namePart><namePart type="family">Silva</namePart><affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Celia</namePart><namePart type="family">Cortez</namePart><affiliation>Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sotiris</namePart><namePart type="family">Missailidis</namePart><affiliation>Department of Chemistry, The Open University, Milton, Keynes MK7 6AA, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Georgia</namePart><namePart type="family">Sotiropoulou</namePart><affiliation>Department of Pharmacy, University of Patras, Rion-Patras 26500, Greece</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Walter de Gruyter</publisher><dateIssued encoding="w3cdtf">2012-05-01</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="references">44</extent></physicalDescription><abstract lang="en">Kallikrein-related peptidase 6 (KLK6) is an active serine protease that has been implicated in common pathologies, including neurodegenerative disorders such as Parkinson and Alzheimer disease and certain types of cancer. Antibodies, either polyclonal or monoclonal, that exhibit specificity for distinct members of the extended kallikrein family, including KLK6, were developed. With the exception of KLK3/PSA, the identification and generation of aptamers, as potential new tools with improved characteristics demanded for therapeutic and diagnostic applications, has not been explored for KLKs. Here, we report for the first time the identification of novel DNA aptamers against KLK6 that were isolated using a modified systemic evolution of ligands by exponential enrichment technique. The identified aptamers were characterized using fluorescence spectroscopy, competition ELISA, and quartz crystal microbalance, and two aptamers (008 and 022) were found to exhibit high affinity (Kd in the low nanomolar range) for KLK6. Aptamers were tested for their ability to bind to serum albumin, to demonstrate their specificity for their target, and the possible involvement of such proteins in the transport of aptamers into the bloodstream. The developed aptamers are expected to assist the development of novel diagnostic, biosensing, and therapeutic strategies.</abstract><note type="author-notes">Corresponding authors</note><subject><genre>Keywords</genre><topic>DNA aptamers</topic><topic>kallikrein-related peptidase 6</topic><topic>SELEX</topic></subject><relatedItem type="host"><titleInfo><title>Biological Chemistry</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">1431-6730</identifier><identifier type="eISSN">1437-4315</identifier><identifier type="PublisherID">bchm</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>393</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>343</start><end>353</end></extent></part></relatedItem><relatedItem type="reviewOf"><genre>pdf</genre><identifier type="pdf">hsz-2011-0253.pdf</identifier></relatedItem><identifier type="istex">131CB1A08EF156270B1029CA47DBCF40E16085E0</identifier><identifier type="DOI">10.1515/hsz-2011-0253</identifier><identifier type="ArticleID">hsz-2011-0253</identifier><identifier type="Related-article-Href">hsz-2011-0253.pdf</identifier><accessCondition type="use and reproduction" contentType="copyright">©2012 by Walter de Gruyter Berlin Boston</accessCondition><recordInfo><recordContentSource>De Gruyter</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D84 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 002D84 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:131CB1A08EF156270B1029CA47DBCF40E16085E0
|texte= One round of SELEX for the generation of DNA aptamers directed against KLK6
}}
| This area was generated with Dilib version V0.6.23. |  |