Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction
Identifieur interne : 002926 ( Main/Corpus ); précédent : 002925; suivant : 002927Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction
Auteurs : M. Maiwald ; M. Schill ; C. Stockinger ; H. Helbig ; C. Lück ; W. Witzleb ; H.-G. SonntagSource :
- European Journal of Clinical Microbiology and Infectious Diseases [ 0934-9723 ] ; 1995-01-01.
Abstract
Abstract: A detection system forLegionella DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extractedLegionella DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with eitherLegionella pneumophila serogroup 1, 3 and 6 orLegionella micdadei, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.Legionella DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate thatLegionella DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of solubleLegionella antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.
Url:
DOI: 10.1007/BF02112614
Links to Exploration step
ISTEX:529657F89D427528C6FCA3BAAE76A19798294280Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title><author><name sortKey="Maiwald, M" sort="Maiwald, M" uniqKey="Maiwald M" first="M." last="Maiwald">M. Maiwald</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schill, M" sort="Schill, M" uniqKey="Schill M" first="M." last="Schill">M. Schill</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Stockinger, C" sort="Stockinger, C" uniqKey="Stockinger C" first="C." last="Stockinger">C. Stockinger</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Helbig, H" sort="Helbig, H" uniqKey="Helbig H" first="H." last="Helbig">H. Helbig</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Luck, C" sort="Luck, C" uniqKey="Luck C" first="C." last="Lück">C. Lück</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Witzleb, W" sort="Witzleb, W" uniqKey="Witzleb W" first="W." last="Witzleb">W. Witzleb</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sonntag, H G" sort="Sonntag, H G" uniqKey="Sonntag H" first="H.-G." last="Sonntag">H.-G. Sonntag</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:529657F89D427528C6FCA3BAAE76A19798294280</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF02112614</idno><idno type="url">https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002926</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title><author><name sortKey="Maiwald, M" sort="Maiwald, M" uniqKey="Maiwald M" first="M." last="Maiwald">M. Maiwald</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schill, M" sort="Schill, M" uniqKey="Schill M" first="M." last="Schill">M. Schill</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Stockinger, C" sort="Stockinger, C" uniqKey="Stockinger C" first="C." last="Stockinger">C. Stockinger</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Helbig, H" sort="Helbig, H" uniqKey="Helbig H" first="H." last="Helbig">H. Helbig</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Luck, C" sort="Luck, C" uniqKey="Luck C" first="C." last="Lück">C. Lück</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Witzleb, W" sort="Witzleb, W" uniqKey="Witzleb W" first="W." last="Witzleb">W. Witzleb</name><affiliation><mods:affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</mods:affiliation></affiliation></author><author><name sortKey="Sonntag, H G" sort="Sonntag, H G" uniqKey="Sonntag H" first="H.-G." last="Sonntag">H.-G. Sonntag</name><affiliation><mods:affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">European Journal of Clinical Microbiology and Infectious Diseases</title><title level="j" type="abbrev">Eur. J. Clin. Microbiol. Infect. Dis.</title><idno type="ISSN">0934-9723</idno><idno type="eISSN">1435-4373</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1995-01-01">1995-01-01</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="25">25</biblScope><biblScope unit="page" to="33">33</biblScope></imprint><idno type="ISSN">0934-9723</idno></series><idno type="istex">529657F89D427528C6FCA3BAAE76A19798294280</idno><idno type="DOI">10.1007/BF02112614</idno><idno type="ArticleID">Art5</idno><idno type="ArticleID">BF02112614</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0934-9723</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A detection system forLegionella DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extractedLegionella DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with eitherLegionella pneumophila serogroup 1, 3 and 6 orLegionella micdadei, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.Legionella DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate thatLegionella DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of solubleLegionella antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>M. Maiwald</name><affiliations><json:string>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>M. Schill</name><affiliations><json:string>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>C. Stockinger</name><affiliations><json:string>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</json:string></affiliations></json:item><json:item><name>J. H. Helbig</name><affiliations><json:string>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</json:string></affiliations></json:item><json:item><name>P. C. Lück</name><affiliations><json:string>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</json:string></affiliations></json:item><json:item><name>W. Witzleb</name><affiliations><json:string>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</json:string></affiliations></json:item><json:item><name>H.-G. Sonntag</name><affiliations><json:string>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</json:string></affiliations></json:item></author><articleId><json:string>Art5</json:string><json:string>BF02112614</json:string></articleId><language><json:string>eng</json:string></language><abstract>Abstract: A detection system forLegionella DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extractedLegionella DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with eitherLegionella pneumophila serogroup 1, 3 and 6 orLegionella micdadei, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.Legionella DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate thatLegionella DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of solubleLegionella antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.</abstract><qualityIndicators><score>7.972</score><pdfVersion>1.3</pdfVersion><pdfPageSize>504 x 756 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1879</abstractCharCount><pdfWordCount>4972</pdfWordCount><pdfCharCount>29402</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>288</abstractWordCount></qualityIndicators><title>Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title><genre><json:string>research-article</json:string></genre><host><volume>14</volume><pages><last>33</last><first>25</first></pages><issn><json:string>0934-9723</json:string></issn><issue>1</issue><subject><json:item><value>Medical Microbiology</value></json:item><json:item><value>Internal Medicine</value></json:item></subject><journalId><json:string>10096</json:string></journalId><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1435-4373</json:string></eissn><title>European Journal of Clinical Microbiology and Infectious Diseases</title><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate></host><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate><doi><json:string>10.1007/BF02112614</json:string></doi><id>529657F89D427528C6FCA3BAAE76A19798294280</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title><respStmt xml:id="ISTEX-API" resp="Références bibliographiques récupérées via GROBID" name="ISTEX-API (INIST-CNRS)"></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><p>SPRINGER</p></availability><date>1995</date></publicationStmt><notesStmt><note>Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title><author><persName><forename type="first">M.</forename><surname>Maiwald</surname></persName><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation></author><author><persName><forename type="first">M.</forename><surname>Schill</surname></persName><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation></author><author><persName><forename type="first">C.</forename><surname>Stockinger</surname></persName><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation></author><author><persName><forename type="first">J.</forename><surname>Helbig</surname></persName><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation></author><author><persName><forename type="first">P.</forename><surname>Lück</surname></persName><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation></author><author><persName><forename type="first">W.</forename><surname>Witzleb</surname></persName><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation></author><author><persName><forename type="first">H.-G.</forename><surname>Sonntag</surname></persName><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation></author></analytic><monogr><title level="j">European Journal of Clinical Microbiology and Infectious Diseases</title><title level="j" type="abbrev">Eur. J. Clin. Microbiol. Infect. Dis.</title><idno type="JournalID">10096</idno><idno type="pISSN">0934-9723</idno><idno type="eISSN">1435-4373</idno><idno type="IssueArticleCount">21</idno><idno type="VolumeIssueCount">12</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1995-01-01"></date><biblScope unit="volume">14</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="25">25</biblScope><biblScope unit="page" to="33">33</biblScope></imprint></monogr><idno type="istex">529657F89D427528C6FCA3BAAE76A19798294280</idno><idno type="DOI">10.1007/BF02112614</idno><idno type="ArticleID">Art5</idno><idno type="ArticleID">BF02112614</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1995</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A detection system forLegionella DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extractedLegionella DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with eitherLegionella pneumophila serogroup 1, 3 and 6 orLegionella micdadei, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.Legionella DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate thatLegionella DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of solubleLegionella antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Medical Microbiology</term></item><item><term>Internal Medicine</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-01-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-3-2">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>10096</JournalID><JournalPrintISSN>0934-9723</JournalPrintISSN><JournalElectronicISSN>1435-4373</JournalElectronicISSN><JournalTitle>European Journal of Clinical Microbiology and Infectious Diseases</JournalTitle><JournalAbbreviatedTitle>Eur. J. Clin. Microbiol. Infect. Dis.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Medical Microbiology</JournalSubject><JournalSubject Type="Secondary">Internal Medicine</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>14</VolumeIDStart><VolumeIDEnd>14</VolumeIDEnd><VolumeIssueCount>12</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>21</IssueArticleCount><IssueHistory><CoverDate><Year>1995</Year><Month>1</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Friedr. Vieweg & Sohn Verlagsgesellschaft mbH</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art5"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF02112614</ArticleID><ArticleDOI>10.1007/BF02112614</ArticleDOI><ArticleSequenceNumber>5</ArticleSequenceNumber><ArticleTitle Language="En">Detection of<Emphasis Type="Italic">Legionella</Emphasis> DNA in human and guinea pig urine samples by the polymerase chain reaction</ArticleTitle><ArticleCategory>Article</ArticleCategory><ArticleFirstPage>25</ArticleFirstPage><ArticleLastPage>33</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>8</Month><Day>6</Day></RegistrationDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Friedr. Vieweg & Sohn Verlagsgesellschaft mbH</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10096</JournalID><VolumeIDStart>14</VolumeIDStart><VolumeIDEnd>14</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Maiwald</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>M.</GivenName><FamilyName>Schill</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>C.</GivenName><FamilyName>Stockinger</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>J.</GivenName><GivenName>H.</GivenName><FamilyName>Helbig</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>P.</GivenName><GivenName>C.</GivenName><FamilyName>Lück</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>W.</GivenName><FamilyName>Witzleb</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>H.-G.</GivenName><FamilyName>Sonntag</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Abteilung Hygiene und Medizinische Mikrobiologie</OrgDivision><OrgName>Hygiene-Institut der Universität</OrgName><OrgAddress><Street>Im Neuenheimer Feld 324</Street><Postcode>69120</Postcode><City>Heidelberg</City><Country>Germany</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Institut für Medizinische Mikrobiologie und Hygiene</OrgDivision><OrgName>Universitätsklinikum der Technischen Universität Dresden</OrgName><OrgAddress><Street>Dürerstr. 24</Street><Postcode>01307</Postcode><City>Dresden</City><Country>Germany</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>A detection system for<Emphasis Type="Italic">Legionella</Emphasis> DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extracted<Emphasis Type="Italic">Legionella</Emphasis> DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with either<Emphasis Type="Italic">Legionella pneumophila</Emphasis> serogroup 1, 3 and 6 or<Emphasis Type="Italic">Legionella micdadei</Emphasis>, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.<Emphasis Type="Italic">Legionella</Emphasis> DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate that<Emphasis Type="Italic">Legionella</Emphasis> DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of soluble<Emphasis Type="Italic">Legionella</Emphasis> antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.</Para></Abstract></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Detection ofLegionella DNA in human and guinea pig urine samples by the polymerase chain reaction</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Maiwald</namePart><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Schill</namePart><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Stockinger</namePart><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="given">H.</namePart><namePart type="family">Helbig</namePart><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="given">C.</namePart><namePart type="family">Lück</namePart><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Witzleb</namePart><affiliation>Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum der Technischen Universität Dresden, Dürerstr. 24, 01307, Dresden, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.-G.</namePart><namePart type="family">Sonntag</namePart><affiliation>Abteilung Hygiene und Medizinische Mikrobiologie, Hygiene-Institut der Universität, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateIssued encoding="w3cdtf">1995-01-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: A detection system forLegionella DNA in urine samples based on the polymerase chain reaction (PCR) was developed and tested on infected guinea pigs and patients suffering from pneumonia. Results were compared with standard methods for diagnosis of Legionnaires' disease. A primer system was selected which amplifies a 108 bp DNA fragment of the 5S rRNA gene. The sensitivity of the PCR system was one femtogram of extractedLegionella DNA. Three methods were tested for pretreatment of urine samples. Of these, the Geneclean II kit (Bio 101, USA) gave the best results for artificially contaminated urine samples as well as those from infected guinea pigs or patients. Thirty-seven urine samples from 15 guinea pigs intraperitoneally infected with eitherLegionella pneumophila serogroup 1, 3 and 6 orLegionella micdadei, 26 urine samples of 21 patients suffering from pneumonia, and 30 control samples of patients with urinary tract infection (UTI) were tested.Legionella DNA was detected in 29 of the guinea pig urine samples; whereas, urinary antigen detection using EIA was positive in only 20 of the samples. PCR was also positive in the samples of 11 patients with pneumonia, 9 of which were confirmed by other microbiological methods, such as culture, direct fluorescent antibody test, urinary antigen detection and antibody testing. However, of the 30 control samples from patients with UTI, three samples yielded positive results. The results demonstrate thatLegionella DNA is excreted in the urine of infected individuals and that the PCR shows a higher degree of sensitivity than EIA to the detection of solubleLegionella antigen in urine. Although the positive results obtained from control samples are a potential limitation of the test, these preliminary data suggest that PCR can be a sensitive method for the diagnosis of legionellosis from urine samples.</abstract><note>Article</note><relatedItem type="host"><titleInfo><title>European Journal of Clinical Microbiology and Infectious Diseases</title></titleInfo><titleInfo type="abbreviated"><title>Eur. J. Clin. Microbiol. Infect. Dis.</title></titleInfo><genre type="Journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1995-01-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Medical Microbiology</topic><topic>Internal Medicine</topic></subject><identifier type="ISSN">0934-9723</identifier><identifier type="eISSN">1435-4373</identifier><identifier type="JournalID">10096</identifier><identifier type="IssueArticleCount">21</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>1995</date><detail type="volume"><number>14</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>25</start><end>33</end></extent></part><recordInfo><recordOrigin>Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, 1995</recordOrigin></recordInfo></relatedItem><identifier type="istex">529657F89D427528C6FCA3BAAE76A19798294280</identifier><identifier type="DOI">10.1007/BF02112614</identifier><identifier type="ArticleID">Art5</identifier><identifier type="ArticleID">BF02112614</identifier><accessCondition type="use and reproduction" contentType="copyright">Friedr. Vieweg & Sohn Verlagsgesellschaft mbH</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, 1995</recordOrigin></recordInfo></mods></metadata><enrichments><istex:refBibTEI uri="https://api.istex.fr/document/529657F89D427528C6FCA3BAAE76A19798294280/enrichments/refBib"><teiHeader></teiHeader><text><front></front><body></body><back><listBibl><biblStruct xml:id="b0"><analytic><title level="a" type="main">Isolation of Legionella pneumophifa from nonepidemic-related aquatic habitats</title><author><persName><forename type="first">Cb</forename><surname>Fliermans</surname></persName></author><author><persName><forename type="first">Wb</forename><surname>Cherry</surname></persName></author><author><persName><forename type="first">Lh</forename><surname>Orrison</surname></persName></author><author><persName><forename type="first">L</forename><surname>Thacker</surname></persName></author></analytic><monogr><title level="j">Applied and Environmental Microbiology</title><imprint><biblScope unit="volume">37</biblScope><biblScope unit="page" from="1239" to="1242"></biblScope><date type="published" when="1979"></date></imprint></monogr></biblStruct><biblStruct xml:id="b1"><analytic><title level="a" type="main">Ecological distribution of Legionella pneumophila</title><author><persName><forename type="first">Cb</forename><surname>Fliermans</surname></persName></author><author><persName><forename type="first">Wb</forename><surname>Cherry</surname></persName></author><author><persName><forename type="first">Lh</forename><surname>Orrison</surname></persName></author><author><persName><forename type="first">S</forename><forename type="middle">J</forename><surname>Smith</surname></persName></author><author><persName><forename type="first">Dl</forename><surname>Tison</surname></persName></author><author><persName><forename type="first">Dh</forename><surname>Pope</surname></persName></author></analytic><monogr><title level="j">Applied and Environmental Microbiology</title><imprint><biblScope unit="volume">41</biblScope><biblScope unit="page" from="9" to="16"></biblScope><date type="published" when="1981"></date></imprint></monogr></biblStruct><biblStruct xml:id="b2"><analytic><title level="a" type="main">Isolation of the Legionnaires' disease bacterium from environmental samples</title><author><persName><forename type="first">Gk</forename><surname>Morris</surname></persName></author><author><persName><forename type="first">Cm</forename><surname>Patton</surname></persName></author><author><persName><forename type="first">Jc</forename><surname>Feeley</surname></persName></author><author><persName><forename type="first">Se</forename><surname>Johnson</surname></persName></author><author><persName><forename type="first">G</forename><surname>G0r-Man</surname></persName></author><author><persName><surname>Martin W-R</surname></persName></author><author><persName><forename type="first">P</forename><surname>Skaliy</surname></persName></author><author><persName><forename type="first">Gf</forename><surname>Mallison</surname></persName></author><author><persName><forename type="first">Bd</forename><surname>Politi</surname></persName></author><author><persName><forename type="first">Dc</forename><surname>Mackel</surname></persName></author></analytic><monogr><title level="j">Annals of Internal Medicine</title><imprint><biblScope unit="volume">90</biblScope><biblScope unit="page" from="664" to="666"></biblScope><date type="published" when="1979"></date></imprint></monogr></biblStruct><biblStruct xml:id="b3"><analytic><title level="a" type="main">Muers MF: Legionnaires' disease in a transplant unit: .isolation of the causative agent from shower baths</title><author><persName><forename type="first">Jo 'h</forename><surname>Tobin</surname></persName></author><author><persName><forename type="first">Ms</forename><surname>Dunnill</surname></persName></author><author><persName><forename type="first">M</forename><surname>French</surname></persName></author><author><persName><forename type="first">Pj</forename><surname>Morris</surname></persName></author><author><persName><forename type="first">J</forename><surname>Beare</surname></persName></author><author><persName><forename type="first">S</forename><surname>Fisher-Hoch</surname></persName></author><author><persName><forename type="first">Rg</forename><surname>Mitchell</surname></persName></author></analytic><monogr><title level="j">Lancet</title><imprint><biblScope unit="volume">ii</biblScope><biblScope unit="page" from="18" to="121"></biblScope><date type="published" when="1980"></date></imprint></monogr></biblStruct><biblStruct xml:id="b4"><analytic><title level="a" type="main">An outbreak of Legionnaires' disease associated with contaminated air conditioning cooling tower</title><author><persName><forename type="first">Tj</forename><surname>Dondero</surname></persName></author><author><persName><forename type="first">Rc</forename><surname>Rendtorff</surname></persName></author><author><persName><forename type="first">Gf</forename><surname>Maliison</surname></persName></author><author><persName><forename type="first">Rm</forename><surname>Weeks</surname></persName></author><author><persName><forename type="first">Js</forename><surname>Levy</surname></persName></author><author><persName><forename type="first">Ew</forename><surname>Wong</surname></persName></author><author><persName><forename type="first">W</forename><surname>Schaffner</surname></persName></author></analytic><monogr><title level="j">New England Journal of Medicine</title><imprint><biblScope unit="volume">302</biblScope><biblScope unit="page" from="365" to="370"></biblScope><date type="published" when="1980"></date></imprint></monogr></biblStruct><biblStruct xml:id="b5"><analytic><title level="a" type="main">Role of air-sampling in an investigation of an outbreak of Legionnaires' disease associated with exposure to aerosols from an evaporative condenser</title><author><persName><forename type="first">Rf</forename><surname>Breiman</surname></persName></author><author><persName><forename type="first">W</forename><surname>Cozen</surname></persName></author><author><persName><forename type="first">Bs</forename><surname>Fields</surname></persName></author><author><persName><forename type="first">Td</forename><surname>Mastro</surname></persName></author><author><persName><forename type="first">S</forename><forename type="middle">J</forename><surname>Carr</surname></persName></author><author><persName><forename type="first">Js</forename><surname>Spika</surname></persName></author><author><persName><forename type="first">L</forename><surname>Mascola</surname></persName></author></analytic><monogr><title level="j">Journal of Infectious Diseases</title><imprint><biblScope unit="volume">161</biblScope><biblScope unit="page" from="1257" to="1261"></biblScope><date type="published" when="1990"></date></imprint></monogr></biblStruct><biblStruct xml:id="b6"><analytic><title level="a" type="main">Potable water as a cause of sporadic cases of community-acquired Legionnaires' disease</title><author><persName><forename type="first">Je</forename><surname>Stout</surname></persName></author><author><persName><forename type="first">Vl</forename><surname>Yu</surname></persName></author><author><persName><forename type="first">P</forename><surname>Muraca</surname></persName></author><author><persName><forename type="first">J</forename><surname>Joly</surname></persName></author><author><persName><forename type="first">N</forename><surname>Troup</surname></persName></author><author><persName><forename type="first">Ls</forename><surname>Tompkins</surname></persName></author></analytic><monogr><title level="j">New England Journal of Medicine</title><imprint><biblScope unit="volume">326</biblScope><biblScope unit="page" from="151" to="155"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b7"><analytic><title level="a" type="main">Nosocomial Legionnaires' disease: aspiration as a primary mode of transmission</title><author><persName><forename type="first">Sp</forename><surname>Blatt</surname></persName></author><author><persName><forename type="first">Md</forename><surname>Parkinson</surname></persName></author><author><persName><forename type="first">E</forename><surname>Pace</surname></persName></author><author><persName><forename type="first">P</forename><surname>Hoffman</surname></persName></author><author><persName><forename type="first">D</forename><surname>Dolan</surname></persName></author><author><persName><forename type="first">P</forename><surname>Lauderdale</surname></persName></author><author><persName><forename type="first">Ra</forename><surname>Zajac</surname></persName></author><author><persName><forename type="first">Gp</forename><surname>Melcher</surname></persName></author></analytic><monogr><title level="j">American Journal of Medicine</title><imprint><biblScope unit="volume">95</biblScope><biblScope unit="page" from="16" to="22"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b8"><analytic><title level="a" type="main">Could aspiration be the major mode of transmission for Legionella?</title><author><persName><forename type="first">Vl</forename><surname>Yu</surname></persName></author></analytic><monogr><title level="j">American Journal of Medicine</title><imprint><biblScope unit="volume">95</biblScope><biblScope unit="page" from="13" to="15"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b9"><analytic><title level="a" type="main">Sporadic and epidemic nosocomial legionellosis in the United States. Epidemiologic features</title><author><persName><forename type="first">Ac</forename><surname>England</surname></persName></author><author><persName><forename type="first">Dw</forename><surname>Fraser</surname></persName></author></analytic><monogr><title level="j">American Journal of Medicine</title><imprint><biblScope unit="volume">70</biblScope><biblScope unit="page" from="707" to="71"></biblScope><date type="published" when="1981"></date></imprint></monogr></biblStruct><biblStruct xml:id="b10"><analytic><title level="a" type="main">A laboratory manual for Legionella</title><author><persName><forename type="first">Tg</forename><surname>Harrison</surname></persName></author><author><persName><forename type="first">Ag</forename><surname>Taylor</surname></persName></author></analytic><monogr><title level="j">Clinical Infectious Diseases</title><imprint><publisher>John Wiley</publisher><publisher>John Wiley</publisher><biblScope unit="volume">16</biblScope><biblScope unit="page" from="741" to="747"></biblScope><date type="published" when="1988"></date></imprint></monogr></biblStruct><biblStruct xml:id="b11"><analytic><title level="a" type="main">Evaluation of sensitivity of two serological tests for diagnosing pneumonia caused by Legionella pneumophila serogroup 1 Aguero-Rosenfeld ME, Edelstein PH: Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans</title><author><persName><forename type="first">Tg</forename><surname>Harrison</surname></persName></author><author><persName><forename type="first">E</forename><surname>Doumon</surname></persName></author><author><persName><forename type="first">Ag</forename><surname>Taylor</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Pathology Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">40</biblScope><biblScope unit="issue">26</biblScope><biblScope unit="page" from="77" to="82"></biblScope><date type="published" when="1987"></date></imprint></monogr></biblStruct><biblStruct xml:id="b12"><analytic><title level="a" type="main">Speciesspecific detection of Legionella pneumophila in water by DNA amplification and hybridization</title><author><persName><forename type="first">Mn</forename><surname>Starnbach</surname></persName></author><author><persName><forename type="first">S</forename><surname>Falkow</surname></persName></author><author><persName><forename type="first">Ls</forename><surname>Tompkins</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">27</biblScope><biblScope unit="page" from="1257" to="1261"></biblScope><date type="published" when="1989"></date></imprint></monogr></biblStruct><biblStruct xml:id="b13"><analytic><title level="a" type="main">Detection of Legionella with polymerase chain reaction and gene probe methods</title><author><persName><forename type="first">Mh</forename><surname>Mahbubani</surname></persName></author><author><persName><forename type="first">Ak</forename><surname>Bej</surname></persName></author><author><persName><forename type="first">R</forename><surname>Miller</surname></persName></author><author><persName><forename type="first">L</forename><surname>Haft</surname></persName></author><author><persName><forename type="first">J</forename><surname>Dicesare</surname></persName></author><author><persName><forename type="first">Rm</forename><surname>Atlas</surname></persName></author></analytic><monogr><title level="j">Molecular and Cellular Probes</title><imprint><biblScope unit="volume">4</biblScope><biblScope unit="page" from="175" to="187"></biblScope><date type="published" when="1990"></date></imprint></monogr></biblStruct><biblStruct xml:id="b14"><analytic><title level="a" type="main">Detection of Legionella spp. in bronchoalveoiar lavage fluids by DNA amplification</title><author><persName><forename type="first">B</forename><surname>Jaulhac</surname></persName></author><author><persName><forename type="first">M</forename><surname>Nowicki</surname></persName></author><author><persName><forename type="first">N</forename><surname>Bornstein</surname></persName></author><author><persName><forename type="first">O</forename><surname>Meunier</surname></persName></author><author><persName><forename type="first">G</forename><surname>Prevost</surname></persName></author><author><persName><forename type="first">Y</forename><surname>Piemont</surname></persName></author><author><persName><forename type="first">Fleurette</forename><forename type="middle">J</forename><surname>Monteit</surname></persName></author><author><persName><forename type="first">H</forename></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">30</biblScope><biblScope unit="page" from="920" to="924"></biblScope><date type="published" when="1992"></date></imprint></monogr></biblStruct><biblStruct xml:id="b15"><analytic><title level="a" type="main">Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method</title><author><persName><forename type="first">M</forename><surname>Koide</surname></persName></author><author><persName><forename type="first">A</forename><surname>Saito</surname></persName></author><author><persName><forename type="first">N</forename><surname>Kusano</surname></persName></author><author><persName><forename type="first">F</forename><surname>Higa</surname></persName></author></analytic><monogr><title level="j">Applied and Environmental Microbiology</title><imprint><biblScope unit="volume">59</biblScope><biblScope unit="page" from="1943" to="1946"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b16"><analytic><title level="a" type="main">Comparison of polymerase chain reaction and conventional culture for the detection of legionellas in hospital water samples</title><author><persName><forename type="first">M</forename><surname>Maiwald</surname></persName></author><author><persName><forename type="first">K</forename><surname>Kissel</surname></persName></author><author><persName><forename type="first">S</forename><surname>Srimuang</surname></persName></author><author><persName><forename type="first">M</forename><surname>Doeberitz</surname></persName></author><author><persName><forename type="first">Hg</forename><surname>Sonntag</surname></persName></author></analytic><monogr><title level="j">Journal of Applied Bacteriology</title><imprint><biblScope unit="volume">76</biblScope><biblScope unit="page" from="216" to="225"></biblScope><date type="published" when="1994"></date></imprint></monogr></biblStruct><biblStruct xml:id="b17"><analytic><title level="a" type="main">Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR amplification and detection kit</title><author><persName><forename type="first">Hh</forename><surname>Kessler</surname></persName></author><author><persName><forename type="first">Ff</forename><surname>Reinthaler</surname></persName></author><author><persName><forename type="first">A</forename><surname>Pschaid</surname></persName></author><author><persName><forename type="first">K</forename><surname>Pierer</surname></persName></author><author><persName><forename type="first">B</forename><surname>Kleinhappt</surname></persName></author><author><persName><forename type="first">E</forename><surname>Eber</surname></persName></author><author><persName><forename type="first">E</forename><surname>Marth</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">31</biblScope><biblScope unit="page" from="3325" to="3328"></biblScope><date type="published" when="1993"></date></imprint></monogr></biblStruct><biblStruct xml:id="b18"><analytic><title level="a" type="main">Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens</title><author><persName><forename type="first">Ph</forename><surname>Edelstein</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">14</biblScope><biblScope unit="page" from="298" to="303"></biblScope><date type="published" when="1981"></date></imprint></monogr></biblStruct><biblStruct xml:id="b19"><monogr><title level="m" type="main">Molecular cloning: a laboratory manual</title><author><persName><forename type="first">Fritsch</forename><surname>Sambrook J</surname></persName></author><author><persName><surname>El=</surname></persName></author><author><persName><forename type="first">T</forename><surname>Maniatis</surname></persName></author><imprint><date type="published" when="1989"></date><publisher>Cold Spring Harbor Laboratory Press</publisher><pubPlace>Cold Spring Harbor, NY</pubPlace></imprint></monogr><note>2nd. edn</note></biblStruct><biblStruct xml:id="b20"><analytic><title level="a" type="main">Identification of a species-specific antigen in Legionella pneumophila by a monoclonal antibody</title><author><persName><forename type="first">Lh</forename><surname>Gosting</surname></persName></author><author><persName><forename type="first">K</forename><surname>Cabrian</surname></persName></author><author><persName><forename type="first">Jc</forename><surname>Sturge</surname></persName></author><author><persName><forename type="first">Lc</forename><surname>Goldstein</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="volume">20</biblScope><biblScope unit="page" from="1031" to="1035"></biblScope><date type="published" when="1984"></date></imprint></monogr></biblStruct><biblStruct xml:id="b21"><monogr><title level="m" type="main">Diagnostik von Legionella-Pneumonien durch Nachweis der Antigenurie mittels Enzymimmunoassay unter Vetwendung von 6 unterschiedlichen Antik6rperspezifit&ten. Zeitschrift for die gesamte Hygiene</title><author><persName><forename type="first">Jh</forename><surname>Helbig</surname></persName></author><author><persName><forename type="first">Pc</forename><surname>Lock</surname></persName></author><author><persName><forename type="first">W</forename><surname>Witzleb</surname></persName></author><imprint><date type="published" when="1989"></date><biblScope unit="page" from="591" to="593"></biblScope></imprint></monogr></biblStruct><biblStruct xml:id="b22"><analytic><title level="a" type="main">Common epitope on urinary antigen derived from different Legionella pneurnophila serogroup 1 strains recognized by a monoclonal antibody</title><author><persName><forename type="first">Jh</forename><surname>Helbig</surname></persName></author><author><persName><forename type="first">Pc</forename><surname>Lock</surname></persName></author><author><persName><forename type="first">C</forename><surname>Pilz</surname></persName></author><author><persName><forename type="first">W</forename><surname>Witzleb</surname></persName></author></analytic><monogr><title level="j">Zentralblatt for Bakteriologie</title><imprint><biblScope unit="volume">273</biblScope><biblScope unit="page" from="478" to="480"></biblScope><date type="published" when="1990"></date></imprint></monogr></biblStruct><biblStruct xml:id="b23"><analytic><title level="a" type="main">Use of slide agglutination test for rapid diagnosis of vaginal candidosis</title><author><persName><forename type="first">R</forename><surname>Rajakumar</surname></persName></author><author><persName><forename type="first">Cjn</forename><surname>Lacey</surname></persName></author><author><persName><forename type="first">Egv</forename><surname>Evans</surname></persName></author><author><persName><forename type="first">Ja</forename><surname>Carney</surname></persName></author></analytic><monogr><title level="j">Genitourinary Medicine</title><imprint><biblScope unit="volume">63</biblScope><biblScope unit="page" from="192" to="195"></biblScope><date type="published" when="1987"></date></imprint></monogr></biblStruct><biblStruct xml:id="b24"><analytic><title level="a" type="main">Evaluation of commercial amplification kit for detection of Legionella pneumophita in clinical specimens</title><author><persName><forename type="first">P</forename><surname>Matsiota-Bemard</surname></persName></author><author><persName><forename type="first">E</forename><surname>Pitsouni</surname></persName></author><author><persName><forename type="first">N</forename><surname>Legakis</surname></persName></author><author><persName><forename type="first">C</forename><surname>Nauciel</surname></persName></author></analytic><monogr><title level="j">Journal of Clinical Microbiology</title><imprint><biblScope unit="page" from="32" to="1503"></biblScope><date type="published" when="1994"></date></imprint></monogr></biblStruct></listBibl></back></text></istex:refBibTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002926 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 002926 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:529657F89D427528C6FCA3BAAE76A19798294280
|texte= Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction
}}
| This area was generated with Dilib version V0.6.23. |  |