monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease
Identifieur interne : 001A60 ( Main/Corpus ); précédent : 001A59; suivant : 001A61monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease
Auteurs : Etienne Hirsch ; Merle Ruberg ; Mireille Dardenne ; Marie-Madeleine Portier ; France Javoy-Agid ; Jean-Francois Bach ; Yves AgidSource :
- Brain Research [ 0006-8993 ] ; 1985.
English descriptors
Abstract
Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.
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DOI: 10.1016/0006-8993(85)91020-0
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U. 25, Hoˆpital Necker, 75007 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Portier, Marie Madeleine" sort="Portier, Marie Madeleine" uniqKey="Portier M" first="Marie-Madeleine" last="Portier">Marie-Madeleine Portier</name><affiliation><mods:affiliation>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</mods:affiliation></affiliation></author><author><name sortKey="Javoy Agid, France" sort="Javoy Agid, France" uniqKey="Javoy Agid F" first="France" last="Javoy-Agid">France Javoy-Agid</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Bach, Jean Francois" sort="Bach, Jean Francois" uniqKey="Bach J" first="Jean-Francois" last="Bach">Jean-Francois Bach</name><affiliation><mods:affiliation>I.N.S.E.R.M. 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U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Ruberg, Merle" sort="Ruberg, Merle" uniqKey="Ruberg M" first="Merle" last="Ruberg">Merle Ruberg</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Dardenne, Mireille" sort="Dardenne, Mireille" uniqKey="Dardenne M" first="Mireille" last="Dardenne">Mireille Dardenne</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Portier, Marie Madeleine" sort="Portier, Marie Madeleine" uniqKey="Portier M" first="Marie-Madeleine" last="Portier">Marie-Madeleine Portier</name><affiliation><mods:affiliation>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</mods:affiliation></affiliation></author><author><name sortKey="Javoy Agid, France" sort="Javoy Agid, France" uniqKey="Javoy Agid F" first="France" last="Javoy-Agid">France Javoy-Agid</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Bach, Jean Francois" sort="Bach, Jean Francois" uniqKey="Bach J" first="Jean-Francois" last="Bach">Jean-Francois Bach</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name><affiliation><mods:affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985">1985</date><biblScope unit="volume">345</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="374">374</biblScope><biblScope unit="page" to="378">378</biblScope></imprint><idno type="ISSN">0006-8993</idno></series><idno type="istex">794BA706B0822803178F782996267BF2F5A4790E</idno><idno type="DOI">10.1016/0006-8993(85)91020-0</idno><idno type="PII">0006-8993(85)91020-0</idno><idno type="ArticleID">85910200</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Lewy body</term><term>Parkinson's disease</term><term>Purkinje cell</term><term>monoclonal antibody</term><term>substantia nigra</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Etienne Hirsch</name><affiliations><json:string>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</json:string></affiliations></json:item><json:item><name>Merle Ruberg</name><affiliations><json:string>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</json:string></affiliations></json:item><json:item><name>Mireille Dardenne</name><affiliations><json:string>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</json:string></affiliations></json:item><json:item><name>Marie-Madeleine Portier</name><affiliations><json:string>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</json:string></affiliations></json:item><json:item><name>France Javoy-Agid</name><affiliations><json:string>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</json:string></affiliations></json:item><json:item><name>Jean-Francois Bach</name><affiliations><json:string>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</json:string></affiliations></json:item><json:item><name>Yves Agid</name><affiliations><json:string>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Lewy body</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Parkinson's disease</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>monoclonal antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>substantia nigra</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Purkinje cell</value></json:item></subject><articleId><json:string>85910200</json:string></articleId><language><json:string>eng</json:string></language><abstract>Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.</abstract><qualityIndicators><score>5.041</score><pdfVersion>1.3</pdfVersion><pdfPageSize>533 x 756 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1116</abstractCharCount><pdfWordCount>3085</pdfWordCount><pdfCharCount>16737</pdfCharCount><pdfPageCount>5</pdfPageCount><abstractWordCount>163</abstractWordCount></qualityIndicators><title>monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease</title><pii><json:string>0006-8993(85)91020-0</json:string></pii><genre><json:string>brief communication</json:string></genre><serie><volume>76</volume><pages><last>4354</last><first>4350</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Proc. 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Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><note type="correspondence"><p>Correspondence: E. Hirsch, I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 91, Boulevard de l'Hoˆpital, 75005 Paris, France.</p></note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation></author><author><persName><forename type="first">Merle</forename><surname>Ruberg</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation></author><author><persName><forename type="first">Mireille</forename><surname>Dardenne</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</affiliation></author><author><persName><forename type="first">Marie-Madeleine</forename><surname>Portier</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</affiliation></author><author><persName><forename type="first">France</forename><surname>Javoy-Agid</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation></author><author><persName><forename type="first">Jean-Francois</forename><surname>Bach</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</affiliation></author><author><persName><forename type="first">Yves</forename><surname>Agid</surname></persName><note type="biography">The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</note><affiliation>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</affiliation><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation></author></analytic><monogr><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="pISSN">0006-8993</idno><idno type="PII">S0006-8993(00)X0937-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1985"></date><biblScope unit="volume">345</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="374">374</biblScope><biblScope unit="page" to="378">378</biblScope></imprint></monogr><idno type="istex">794BA706B0822803178F782996267BF2F5A4790E</idno><idno type="DOI">10.1016/0006-8993(85)91020-0</idno><idno type="PII">0006-8993(85)91020-0</idno><idno type="ArticleID">85910200</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1985</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Lewy body</term></item><item><term>Parkinson's disease</term></item><item><term>monoclonal antibody</term></item><item><term>substantia nigra</term></item><item><term>Purkinje cell</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1985-05-29">Registration</change><change when="1985">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="sco"><item-info><jid>BRES</jid><aid>85910200</aid><ce:pii>0006-8993(85)91020-0</ce:pii><ce:doi>10.1016/0006-8993(85)91020-0</ce:doi><ce:copyright type="unknown" year="1985">Elsevier Science Publishers B.V.</ce:copyright></item-info><head><ce:title>monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease</ce:title><ce:author-group><ce:author><ce:given-name>Etienne</ce:given-name><ce:surname>Hirsch</ce:surname><ce:cross-ref refid="cor1"><ce:sup loc="post">*</ce:sup></ce:cross-ref><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Merle</ce:given-name><ce:surname>Ruberg</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Mireille</ce:given-name><ce:surname>Dardenne</ce:surname><ce:cross-ref refid="aff2"><ce:sup loc="post">2</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Marie-Madeleine</ce:given-name><ce:surname>Portier</ce:surname><ce:cross-ref refid="aff3"><ce:sup loc="post">3</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>France</ce:given-name><ce:surname>Javoy-Agid</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean-Francois</ce:given-name><ce:surname>Bach</ce:surname><ce:cross-ref refid="aff2"><ce:sup loc="post">2</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Yves</ce:given-name><ce:surname>Agid</ce:surname><ce:cross-ref refid="aff1"><ce:sup loc="post">1</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup loc="post">1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>a</ce:label><ce:textfn>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>b</ce:label><ce:textfn>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="aff3"><ce:label>c</ce:label><ce:textfn>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*</ce:label><ce:text><ce:italic>Correspondence:</ce:italic> E. Hirsch, I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 91, Boulevard de l'Hoˆpital, 75005 Paris, France.</ce:text></ce:correspondence><ce:footnote id="fn1"><ce:label>1</ce:label><ce:note-para>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</ce:note-para></ce:footnote></ce:author-group><ce:date-accepted day="29" month="5" year="1985"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. 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Neurol.</sb:maintitle></sb:title><sb:volume-nr>132</sb:volume-nr></sb:series><sb:date>1983</sb:date></sb:issue><sb:pages><sb:first-page>243</sb:first-page><sb:last-page>248</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease</title></titleInfo><name type="personal"><namePart type="given">Etienne</namePart><namePart type="family">Hirsch</namePart><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation><description>Correspondence: E. Hirsch, I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 91, Boulevard de l'Hoˆpital, 75005 Paris, France.</description><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Merle</namePart><namePart type="family">Ruberg</namePart><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mireille</namePart><namePart type="family">Dardenne</namePart><affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marie-Madeleine</namePart><namePart type="family">Portier</namePart><affiliation>Laboratoire de Biochimie Cellulaire, Collège de France, 75005 Paris France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">France</namePart><namePart type="family">Javoy-Agid</namePart><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Francois</namePart><namePart type="family">Bach</namePart><affiliation>I.N.S.E.R.M. U. 25, Hoˆpital Necker, 75007 Paris, France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yves</namePart><namePart type="family">Agid</namePart><affiliation>I.N.S.E.R.M. U. 289, Hoˆpital de la Salpêtrière, 75005 Paris, France</affiliation><description>The authors would like to thank N. Le Balc'h and M. C. Gagnerault for their help in performing these experiments.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief communication" displayLabel="Short communication"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1985</dateIssued><dateValid encoding="w3cdtf">1985-05-29</dateValid><copyrightDate encoding="w3cdtf">1985</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.</abstract><subject lang="en"><genre>Keywords</genre><topic>Lewy body</topic><topic>Parkinson's disease</topic><topic>monoclonal antibody</topic><topic>substantia nigra</topic><topic>Purkinje cell</topic></subject><relatedItem type="host"><titleInfo><title>Brain Research</title></titleInfo><titleInfo type="abbreviated"><title>BRES</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19851021</dateIssued></originInfo><identifier type="ISSN">0006-8993</identifier><identifier type="PII">S0006-8993(00)X0937-7</identifier><part><detail type="volume"><number>345</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>205</start><end>412</end></extent><extent unit="pages"><start>374</start><end>378</end></extent></part></relatedItem><identifier type="istex">794BA706B0822803178F782996267BF2F5A4790E</identifier><identifier type="DOI">10.1016/0006-8993(85)91020-0</identifier><identifier type="PII">0006-8993(85)91020-0</identifier><identifier type="ArticleID">85910200</identifier><accessCondition type="use and reproduction" contentType="">© 1985Elsevier Science Publishers B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Publishers B.V., ©1985</recordOrigin></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/794BA706B0822803178F782996267BF2F5A4790E/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">NEUROSCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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