Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
Identifieur interne : 001058 ( Main/Corpus ); précédent : 001057; suivant : 001059Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
Auteurs : Jong-Hoon Kim ; Jonathan M. Auerbach ; Jos A. Rodrguez-Gmez ; Ivn Velasco ; Denise Gavin ; Nadya Lumelsky ; Sang-Hun Lee ; John Nguyen ; Rosario Snchez-Pernaute ; Krys Bankiewicz ; Ron MckaySource :
- Nature [ 0028-0836 ] ; 2002-07-04.
Abstract
Parkinson's disease is a widespread condition caused by the loss of midbrain neurons that synthesize the neurotransmitter dopamine. Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease.
Url:
DOI: 10.1038/nature00900
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease</title><author><name sortKey="Kim, Jong Hoon" sort="Kim, Jong Hoon" uniqKey="Kim J" first="Jong-Hoon" last="Kim">Jong-Hoon Kim</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Auerbach, Jonathan M" sort="Auerbach, Jonathan M" uniqKey="Auerbach J" first="Jonathan M." last="Auerbach">Jonathan M. Auerbach</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Rodrguez Gmez, Jos A" sort="Rodrguez Gmez, Jos A" uniqKey="Rodrguez Gmez J" first="Jos A." last="Rodrguez-Gmez">Jos A. Rodrguez-Gmez</name></author><author><name sortKey="Velasco, Ivn" sort="Velasco, Ivn" uniqKey="Velasco I" first="Ivn" last="Velasco">Ivn Velasco</name></author><author><name sortKey="Gavin, Denise" sort="Gavin, Denise" uniqKey="Gavin D" first="Denise" last="Gavin">Denise Gavin</name></author><author><name sortKey="Lumelsky, Nadya" sort="Lumelsky, Nadya" uniqKey="Lumelsky N" first="Nadya" last="Lumelsky">Nadya Lumelsky</name></author><author><name sortKey="Lee, Sang Hun" sort="Lee, Sang Hun" uniqKey="Lee S" first="Sang-Hun" last="Lee">Sang-Hun Lee</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Nguyen, John" sort="Nguyen, John" uniqKey="Nguyen J" first="John" last="Nguyen">John Nguyen</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Snchez Pernaute, Rosario" sort="Snchez Pernaute, Rosario" uniqKey="Snchez Pernaute R" first="Rosario" last="Snchez-Pernaute">Rosario Snchez-Pernaute</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Bankiewicz, Krys" sort="Bankiewicz, Krys" uniqKey="Bankiewicz K" first="Krys" last="Bankiewicz">Krys Bankiewicz</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Mckay, Ron" sort="Mckay, Ron" uniqKey="Mckay R" first="Ron" last="Mckay">Ron Mckay</name><affiliation><mods:affiliation>E-mail: mckay@codon.nih.gov</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:91DFD68C09793A588706E6A511D2E40D9AEE6B18</idno><date when="2002" year="2002">2002</date><idno type="doi">10.1038/nature00900</idno><idno type="url">https://api.istex.fr/document/91DFD68C09793A588706E6A511D2E40D9AEE6B18/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">001058</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease</title><author><name sortKey="Kim, Jong Hoon" sort="Kim, Jong Hoon" uniqKey="Kim J" first="Jong-Hoon" last="Kim">Jong-Hoon Kim</name><affiliation><mods:affiliation>These authors contributed equally to this work.</mods:affiliation></affiliation></author><author><name sortKey="Auerbach, Jonathan M" sort="Auerbach, Jonathan M" uniqKey="Auerbach J" first="Jonathan M." last="Auerbach">Jonathan M. 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Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease.</div></front></TEI><istex><corpusName>nature</corpusName><author><json:item><name>Jong-Hoon Kim</name><affiliations><json:string>These authors contributed equally to this work.</json:string></affiliations></json:item><json:item><name>Jonathan M. Auerbach</name><affiliations><json:string>These authors contributed equally to this work.</json:string><json:string>These authors contributed equally to this work.</json:string></affiliations></json:item><json:item><name>Jos A. 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(e-mail: <email>mckay@codon.nih.gov</email>).</caff></aug><hst><re year="2002" month="4" day="24"></re><acc year="2002" month="6" day="12"></acc><pubdate type="aop" year="2002" month="06" day="20"></pubdate></hst><abs><p>Parkinson's disease is a widespread condition caused by the loss of midbrain neurons that synthesize the neurotransmitter dopamine. Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease.</p></abs></fm></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="eng"><title>Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease</title></titleInfo><titleInfo type="alternative" lang="eng" contentType="CDATA"><title>Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease</title></titleInfo><name type="personal"><namePart type="given">Jong-Hoon</namePart><namePart type="family">Kim</namePart><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jonathan M.</namePart><namePart type="family">Auerbach</namePart><affiliation>These authors contributed equally to this work.</affiliation><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jos A.</namePart><namePart type="family">Rodrguez-Gmez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ivn</namePart><namePart type="family">Velasco</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Denise</namePart><namePart type="family">Gavin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nadya</namePart><namePart type="family">Lumelsky</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sang-Hun</namePart><namePart type="family">Lee</namePart><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John</namePart><namePart type="family">Nguyen</namePart><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rosario</namePart><namePart type="family">Snchez-Pernaute</namePart><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Krys</namePart><namePart type="family">Bankiewicz</namePart><affiliation>These authors contributed equally to this work.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ron</namePart><namePart type="family">McKay</namePart><affiliation>E-mail: mckay@codon.nih.gov</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Article"></genre><originInfo><publisher>Macmillian Magazines Ltd.</publisher><dateIssued encoding="w3cdtf">2002-07-04</dateIssued><dateCaptured encoding="w3cdtf">2002-04-24</dateCaptured><dateValid encoding="w3cdtf">2002-06-12</dateValid><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="eng">Parkinson's disease is a widespread condition caused by the loss of midbrain neurons that synthesize the neurotransmitter dopamine. Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease.</abstract><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><identifier type="ISSN">0028-0836</identifier><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>418</number></detail><detail type="issue"><caption>no.</caption><number>6893</number></detail><extent unit="pages"><start>50</start><end>56</end></extent></part></relatedItem><identifier type="istex">91DFD68C09793A588706E6A511D2E40D9AEE6B18</identifier><identifier type="DOI">10.1038/nature00900</identifier><identifier type="ArticleID">nature00900</identifier><accessCondition type="use and reproduction" contentType="copyright">©2002 Macmillian Magazines Ltd.</accessCondition><recordInfo><recordContentSource>NATURE</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/91DFD68C09793A588706E6A511D2E40D9AEE6B18/covers/jpeg</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/91DFD68C09793A588706E6A511D2E40D9AEE6B18/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/91DFD68C09793A588706E6A511D2E40D9AEE6B18/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/91DFD68C09793A588706E6A511D2E40D9AEE6B18/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">MULTIDISCIPLINARY SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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