Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract
Identifieur interne : 000D04 ( Main/Corpus ); précédent : 000D03; suivant : 000D05Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract
Auteurs : Nadja Van Camp ; Ines Blockx ; Marleen Verhoye ; Cindy Casteels ; Frea Coun ; Alexander Leemans ; Jan Sijbers ; Veerle Baekelandt ; Koen Van Laere ; Annemie Van Der LindenSource :
- NMR in Biomedicine [ 0952-3480 ] ; 2009-08.
English descriptors
- KwdEn :
Abstract
Parkinson's disease (PD) is characterised by degeneration of the nigrostrial connection causing dramatic changes in the dopaminergic pathway underlying clinical pathology. Till now, no MRI tools were available to follow up any specific PD‐related neurodegeneration. However, recently, diffusion tensor imaging (DTI) has received considerable attention as a new and potential in vivo diagnostic tool for various neurodegenerative diseases. To assess this in PD, we performed DTI in the acute 6‐hydroxydopamine (6‐OHDA) rat model of PD to evaluate diffusion properties in the degenerating nigrostriatal pathway and its connecting structures. Injection of a neurotoxin in the striatum causes retrograde neurodegeneration of the nigrostriatal tract, and selective degeneration of nigral neurons. The advantage of this model is that the lesion size is well controllable by the injected dose of the toxin. The degree of functional impairment was evaluated in vivo using the amphetamine rotation test and µPET imaging of the dopamine transporter (DAT). Despite a nearly complete lesion of the nigrostriatal tract, DTI changes were limited to the ipsilateral substantia nigra (SN). In this study we demonstrate, using voxel‐based statistics (VBS), an increase in fractional anisotropy (FA), whereas all eigenvalues were significantly decreased. VBS enabled us to visualise neurodegeneration of a cluster of neurons but failed to detect degeneration of more diffuse microstructures such as the nigrostriatal fibres or the dopaminergic endings in the striatum. VBS without a priori information proved to be better than manual segmentation of brain structures as it does not suffer from volume averaging and is not susceptible to erroneous segmentations of brain regions that show very little contrast on MRI images such as SN. Copyright © 2009 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/nbm.1381
Links to Exploration step
ISTEX:E5C66EB71369FC9AE2C30D95DBA381175DB6E0FALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title><author><name sortKey="Van Camp, Nadja" sort="Van Camp, Nadja" uniqKey="Van Camp N" first="Nadja" last="Van Camp">Nadja Van Camp</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</mods:affiliation></affiliation><affiliation><mods:affiliation>INSERM, U803, Orsay, France</mods:affiliation></affiliation></author><author><name sortKey="Blockx, Ines" sort="Blockx, Ines" uniqKey="Blockx I" first="Ines" last="Blockx">Ines Blockx</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Verhoye, Marleen" sort="Verhoye, Marleen" uniqKey="Verhoye M" first="Marleen" last="Verhoye">Marleen Verhoye</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Casteels, Cindy" sort="Casteels, Cindy" uniqKey="Casteels C" first="Cindy" last="Casteels">Cindy Casteels</name><affiliation><mods:affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Coun, Frea" sort="Coun, Frea" uniqKey="Coun F" first="Frea" last="Coun">Frea Coun</name><affiliation><mods:affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Leemans, Alexander" sort="Leemans, Alexander" uniqKey="Leemans A" first="Alexander" last="Leemans">Alexander Leemans</name><affiliation><mods:affiliation>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Sijbers, Jan" sort="Sijbers, Jan" uniqKey="Sijbers J" first="Jan" last="Sijbers">Jan Sijbers</name><affiliation><mods:affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Baekelandt, Veerle" sort="Baekelandt, Veerle" uniqKey="Baekelandt V" first="Veerle" last="Baekelandt">Veerle Baekelandt</name><affiliation><mods:affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Van Laere, Koen" sort="Van Laere, Koen" uniqKey="Van Laere K" first="Koen" last="Van Laere">Koen Van Laere</name><affiliation><mods:affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Van Der Linden, Annemie" sort="Van Der Linden, Annemie" uniqKey="Van Der Linden A" first="Annemie" last="Van Der Linden">Annemie Van Der Linden</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1002/nbm.1381</idno><idno type="url">https://api.istex.fr/document/E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">000D04</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title><author><name sortKey="Van Camp, Nadja" sort="Van Camp, Nadja" uniqKey="Van Camp N" first="Nadja" last="Van Camp">Nadja Van Camp</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</mods:affiliation></affiliation><affiliation><mods:affiliation>INSERM, U803, Orsay, France</mods:affiliation></affiliation></author><author><name sortKey="Blockx, Ines" sort="Blockx, Ines" uniqKey="Blockx I" first="Ines" last="Blockx">Ines Blockx</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Verhoye, Marleen" sort="Verhoye, Marleen" uniqKey="Verhoye M" first="Marleen" last="Verhoye">Marleen Verhoye</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Casteels, Cindy" sort="Casteels, Cindy" uniqKey="Casteels C" first="Cindy" last="Casteels">Cindy Casteels</name><affiliation><mods:affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Coun, Frea" sort="Coun, Frea" uniqKey="Coun F" first="Frea" last="Coun">Frea Coun</name><affiliation><mods:affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Leemans, Alexander" sort="Leemans, Alexander" uniqKey="Leemans A" first="Alexander" last="Leemans">Alexander Leemans</name><affiliation><mods:affiliation>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Sijbers, Jan" sort="Sijbers, Jan" uniqKey="Sijbers J" first="Jan" last="Sijbers">Jan Sijbers</name><affiliation><mods:affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Baekelandt, Veerle" sort="Baekelandt, Veerle" uniqKey="Baekelandt V" first="Veerle" last="Baekelandt">Veerle Baekelandt</name><affiliation><mods:affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Van Laere, Koen" sort="Van Laere, Koen" uniqKey="Van Laere K" first="Koen" last="Van Laere">Koen Van Laere</name><affiliation><mods:affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Van Der Linden, Annemie" sort="Van Der Linden, Annemie" uniqKey="Van Der Linden A" first="Annemie" last="Van Der Linden">Annemie Van Der Linden</name><affiliation><mods:affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">NMR in Biomedicine</title><title level="j" type="sub">An International Journal Devoted to the Development and Application of Magnetic Resonance In vivo</title><title level="j" type="abbrev">NMR Biomed.</title><idno type="ISSN">0952-3480</idno><idno type="eISSN">1099-1492</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2009-08">2009-08</date><biblScope unit="volume">22</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="697">697</biblScope><biblScope unit="page" to="706">706</biblScope></imprint><idno type="ISSN">0952-3480</idno></series><idno type="istex">E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA</idno><idno type="DOI">10.1002/nbm.1381</idno><idno type="ArticleID">NBM1381</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0952-3480</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>6‐OHDA rat model</term><term>DTI</term><term>Parkinson's disease</term><term>neurodegeneration</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Parkinson's disease (PD) is characterised by degeneration of the nigrostrial connection causing dramatic changes in the dopaminergic pathway underlying clinical pathology. Till now, no MRI tools were available to follow up any specific PD‐related neurodegeneration. However, recently, diffusion tensor imaging (DTI) has received considerable attention as a new and potential in vivo diagnostic tool for various neurodegenerative diseases. To assess this in PD, we performed DTI in the acute 6‐hydroxydopamine (6‐OHDA) rat model of PD to evaluate diffusion properties in the degenerating nigrostriatal pathway and its connecting structures. Injection of a neurotoxin in the striatum causes retrograde neurodegeneration of the nigrostriatal tract, and selective degeneration of nigral neurons. The advantage of this model is that the lesion size is well controllable by the injected dose of the toxin. The degree of functional impairment was evaluated in vivo using the amphetamine rotation test and µPET imaging of the dopamine transporter (DAT). Despite a nearly complete lesion of the nigrostriatal tract, DTI changes were limited to the ipsilateral substantia nigra (SN). In this study we demonstrate, using voxel‐based statistics (VBS), an increase in fractional anisotropy (FA), whereas all eigenvalues were significantly decreased. VBS enabled us to visualise neurodegeneration of a cluster of neurons but failed to detect degeneration of more diffuse microstructures such as the nigrostriatal fibres or the dopaminergic endings in the striatum. VBS without a priori information proved to be better than manual segmentation of brain structures as it does not suffer from volume averaging and is not susceptible to erroneous segmentations of brain regions that show very little contrast on MRI images such as SN. Copyright © 2009 John Wiley & Sons, Ltd.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Nadja Van Camp</name><affiliations><json:string>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</json:string><json:string>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</json:string><json:string>INSERM, U803, Orsay, France</json:string></affiliations></json:item><json:item><name>Ines Blockx</name><affiliations><json:string>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</json:string></affiliations></json:item><json:item><name>Marleen Verhoye</name><affiliations><json:string>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</json:string><json:string>Vision Laboratory, University of Antwerp, Antwerp, Belgium</json:string></affiliations></json:item><json:item><name>Cindy Casteels</name><affiliations><json:string>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Frea Coun</name><affiliations><json:string>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Alexander Leemans</name><affiliations><json:string>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</json:string><json:string>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>Jan Sijbers</name><affiliations><json:string>Vision Laboratory, University of Antwerp, Antwerp, Belgium</json:string></affiliations></json:item><json:item><name>Veerle Baekelandt</name><affiliations><json:string>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Koen Van Laere</name><affiliations><json:string>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Annemie Van der Linden</name><affiliations><json:string>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>DTI</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Parkinson's disease</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>6‐OHDA rat model</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>neurodegeneration</value></json:item></subject><articleId><json:string>NBM1381</json:string></articleId><language><json:string>eng</json:string></language><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595 x 791 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>1857</abstractCharCount><pdfWordCount>6827</pdfWordCount><pdfCharCount>42597</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>274</abstractWordCount></qualityIndicators><title>Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title><genre><json:string>article</json:string></genre><host><volume>22</volume><publisherId><json:string>NBM</json:string></publisherId><pages><total>10</total><last>706</last><first>697</first></pages><issn><json:string>0952-3480</json:string></issn><issue>7</issue><subject><json:item><value>Research Article</value></json:item></subject><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1099-1492</json:string></eissn><title>NMR in Biomedicine</title><doi><json:string>10.1002/(ISSN)1099-1492</json:string></doi></host><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1002/nbm.1381</json:string></doi><id>E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><availability><p>WILEY</p></availability><date>2009</date></publicationStmt><notesStmt><note>The EC‐FP6‐project DiMI - No. LSHB‐CT‐2005‐512146;</note><note>The EC‐FP6‐project EMIL - No. LSHC‐CT‐2004‐503569;</note><note>The Institute for the Promotion of Innovation by Science and Technology in Flanders - No. IWT SBO/030238;</note><note>The European Community RATstreamTM STREP - No. LSHM‐CT‐2007‐037846;</note><note>Inter University Attraction Poles - No. IUAP‐NIMI‐P6/38;</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title><author><persName><forename type="first">Nadja</forename><surname>Van Camp</surname></persName><note type="correspondence"><p>Correspondence: Bio‐Imaging Laboratory, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.</p></note><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><affiliation>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</affiliation><affiliation>INSERM, U803, Orsay, France</affiliation></author><author><persName><forename type="first">Ines</forename><surname>Blockx</surname></persName><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation></author><author><persName><forename type="first">Marleen</forename><surname>Verhoye</surname></persName><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</affiliation></author><author><persName><forename type="first">Cindy</forename><surname>Casteels</surname></persName><affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</affiliation></author><author><persName><forename type="first">Frea</forename><surname>Coun</surname></persName><affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</affiliation></author><author><persName><forename type="first">Alexander</forename><surname>Leemans</surname></persName><affiliation>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</affiliation><affiliation>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</affiliation></author><author><persName><forename type="first">Jan</forename><surname>Sijbers</surname></persName><affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</affiliation></author><author><persName><forename type="first">Veerle</forename><surname>Baekelandt</surname></persName><affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</affiliation></author><author><persName><forename type="first">Koen</forename><surname>Van Laere</surname></persName><affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</affiliation></author><author><persName><forename type="first">Annemie</forename><surname>Van der Linden</surname></persName><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation></author></analytic><monogr><title level="j">NMR in Biomedicine</title><title level="j" type="sub">An International Journal Devoted to the Development and Application of Magnetic Resonance In vivo</title><title level="j" type="abbrev">NMR Biomed.</title><idno type="pISSN">0952-3480</idno><idno type="eISSN">1099-1492</idno><idno type="DOI">10.1002/(ISSN)1099-1492</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2009-08"></date><biblScope unit="volume">22</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="697">697</biblScope><biblScope unit="page" to="706">706</biblScope></imprint></monogr><idno type="istex">E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA</idno><idno type="DOI">10.1002/nbm.1381</idno><idno type="ArticleID">NBM1381</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Parkinson's disease (PD) is characterised by degeneration of the nigrostrial connection causing dramatic changes in the dopaminergic pathway underlying clinical pathology. Till now, no MRI tools were available to follow up any specific PD‐related neurodegeneration. However, recently, diffusion tensor imaging (DTI) has received considerable attention as a new and potential in vivo diagnostic tool for various neurodegenerative diseases. To assess this in PD, we performed DTI in the acute 6‐hydroxydopamine (6‐OHDA) rat model of PD to evaluate diffusion properties in the degenerating nigrostriatal pathway and its connecting structures. Injection of a neurotoxin in the striatum causes retrograde neurodegeneration of the nigrostriatal tract, and selective degeneration of nigral neurons. The advantage of this model is that the lesion size is well controllable by the injected dose of the toxin. The degree of functional impairment was evaluated in vivo using the amphetamine rotation test and µPET imaging of the dopamine transporter (DAT). Despite a nearly complete lesion of the nigrostriatal tract, DTI changes were limited to the ipsilateral substantia nigra (SN). In this study we demonstrate, using voxel‐based statistics (VBS), an increase in fractional anisotropy (FA), whereas all eigenvalues were significantly decreased. VBS enabled us to visualise neurodegeneration of a cluster of neurons but failed to detect degeneration of more diffuse microstructures such as the nigrostriatal fibres or the dopaminergic endings in the striatum. VBS without a priori information proved to be better than manual segmentation of brain structures as it does not suffer from volume averaging and is not susceptible to erroneous segmentations of brain regions that show very little contrast on MRI images such as SN. Copyright © 2009 John Wiley & Sons, Ltd.</p></abstract><abstract xml:lang="en" style="graphical"><p>DTI was performed in the 6‐hydroxydopamine rat model of Parkinson's disease to evaluate the degeneration of the nigrostriatal pathway. We demonstrated a significant increase in fractional anisotropy, and decrease of all eigenvalues in the SN. Voxel‐based statistics proved to be better than manual segmentation of brain structures as they do not suffer from volume averaging and are not susceptible to erroneous segmentation of brain regions.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>DTI</term></item><item><term>Parkinson's disease</term></item><item><term>6‐OHDA rat model</term></item><item><term>neurodegeneration</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-09-22">Received</change><change when="2009-02-03">Registration</change><change when="2009-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>John Wiley & Sons, Ltd.</publisherName><publisherLoc>Chichester, UK</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1099-1492</doi><issn type="print">0952-3480</issn><issn type="electronic">1099-1492</issn><idGroup><id type="product" value="NBM"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="NMR IN BIOMEDICINE">NMR in Biomedicine</title><title type="subtitle">An International Journal Devoted to the Development and Application of Magnetic Resonance <i>In vivo</i></title><title type="short">NMR Biomed.</title></titleGroup></publicationMeta><publicationMeta level="part" position="70"><doi origin="wiley" registered="yes">10.1002/nbm.v22:7</doi><numberingGroup><numbering type="journalVolume" number="22">22</numbering><numbering type="journalIssue">7</numbering></numberingGroup><coverDate startDate="2009-08">August 2009</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="2" status="forIssue"><doi origin="wiley" registered="yes">10.1002/nbm.1381</doi><idGroup><id type="unit" value="NBM1381"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2009 John Wiley & Sons, Ltd.</copyright><eventGroup><event type="manuscriptReceived" date="2008-09-22"></event><event type="manuscriptRevised" date="2009-02-03"></event><event type="manuscriptAccepted" date="2009-02-03"></event><event type="publishedOnlineEarlyUnpaginated" date="2009-04-17"></event><event type="firstOnline" date="2009-04-17"></event><event type="publishedOnlineFinalForm" date="2009-08-07"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-16"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-03"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst">697</numbering><numbering type="pageLast">706</numbering></numberingGroup><correspondenceTo>Bio‐Imaging Laboratory, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:NBM.NBM1381.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="2"></count><count type="referenceTotal" number="45"></count></countGroup><titleGroup><title type="main" xml:lang="en">Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title><title type="short" xml:lang="en">DTI IN THE 6OHDA RAT MODEL OF PARKINSON'S DISEASE</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2 #af3" corresponding="yes"><personName><givenNames>Nadja</givenNames><familyName>Van Camp</familyName></personName><contactDetails><email>nadja.vancamp@ua.ac.be</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Ines</givenNames><familyName>Blockx</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1 #af4"><personName><givenNames>Marleen</givenNames><familyName>Verhoye</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Cindy</givenNames><familyName>Casteels</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af6"><personName><givenNames>Frea</givenNames><familyName>Coun</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af7 #af8"><personName><givenNames>Alexander</givenNames><familyName>Leemans</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Jan</givenNames><familyName>Sijbers</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af6"><personName><givenNames>Veerle</givenNames><familyName>Baekelandt</familyName></personName></creator><creator xml:id="au9" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Koen</givenNames><familyName>Van Laere</familyName></personName></creator><creator xml:id="au10" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Annemie</givenNames><familyName>Van der Linden</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="BE" type="organization"><unparsedAffiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="FR" type="organization"><unparsedAffiliation>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="FR" type="organization"><unparsedAffiliation>INSERM, U803, Orsay, France</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="BE" type="organization"><unparsedAffiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="BE" type="organization"><unparsedAffiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="af6" countryCode="BE" type="organization"><unparsedAffiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</unparsedAffiliation></affiliation><affiliation xml:id="af7" countryCode="GB" type="organization"><unparsedAffiliation>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</unparsedAffiliation></affiliation><affiliation xml:id="af8" countryCode="NL" type="organization"><unparsedAffiliation>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">DTI</keyword><keyword xml:id="kwd2">Parkinson's disease</keyword><keyword xml:id="kwd3">6‐OHDA rat model</keyword><keyword xml:id="kwd4">neurodegeneration</keyword></keywordGroup><fundingInfo><fundingAgency>The EC‐FP6‐project DiMI</fundingAgency><fundingNumber>LSHB‐CT‐2005‐512146</fundingNumber></fundingInfo><fundingInfo><fundingAgency>The EC‐FP6‐project EMIL</fundingAgency><fundingNumber>LSHC‐CT‐2004‐503569</fundingNumber></fundingInfo><fundingInfo><fundingAgency>The Institute for the Promotion of Innovation by Science and Technology in Flanders</fundingAgency><fundingNumber>IWT SBO/030238</fundingNumber></fundingInfo><fundingInfo><fundingAgency>The European Community RATstreamTM STREP</fundingAgency><fundingNumber>LSHM‐CT‐2007‐037846</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Inter University Attraction Poles</fundingAgency><fundingNumber>IUAP‐NIMI‐P6/38</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Parkinson's disease (PD) is characterised by degeneration of the nigrostrial connection causing dramatic changes in the dopaminergic pathway underlying clinical pathology. Till now, no MRI tools were available to follow up any specific PD‐related neurodegeneration. However, recently, diffusion tensor imaging (DTI) has received considerable attention as a new and potential <i>in vivo</i> diagnostic tool for various neurodegenerative diseases. To assess this in PD, we performed DTI in the acute 6‐hydroxydopamine (6‐OHDA) rat model of PD to evaluate diffusion properties in the degenerating nigrostriatal pathway and its connecting structures. Injection of a neurotoxin in the striatum causes retrograde neurodegeneration of the nigrostriatal tract, and selective degeneration of nigral neurons. The advantage of this model is that the lesion size is well controllable by the injected dose of the toxin. The degree of functional impairment was evaluated <i>in vivo</i> using the amphetamine rotation test and <i>µ</i>PET imaging of the dopamine transporter (DAT). Despite a nearly complete lesion of the nigrostriatal tract, DTI changes were limited to the ipsilateral substantia nigra (SN). In this study we demonstrate, using voxel‐based statistics (VBS), an increase in fractional anisotropy (FA), whereas all eigenvalues were significantly decreased. VBS enabled us to visualise neurodegeneration of a cluster of neurons but failed to detect degeneration of more diffuse microstructures such as the nigrostriatal fibres or the dopaminergic endings in the striatum. VBS without <i>a priori</i> information proved to be better than manual segmentation of brain structures as it does not suffer from volume averaging and is not susceptible to erroneous segmentations of brain regions that show very little contrast on MRI images such as SN. Copyright © 2009 John Wiley & Sons, Ltd.</p></abstract><abstract type="graphical" xml:lang="en"><p>DTI was performed in the 6‐hydroxydopamine rat model of Parkinson's disease to evaluate the degeneration of the nigrostriatal pathway. We demonstrated a significant increase in fractional anisotropy, and decrease of all eigenvalues in the SN. Voxel‐based statistics proved to be better than manual segmentation of brain structures as they do not suffer from volume averaging and are not susceptible to erroneous segmentation of brain regions. <blockFixed type="graphic"><mediaResourceGroup><mediaResource alt="image" eRights="yes" copyright="John Wiley & Sons, Ltd." href="urn:x-wiley:09523480:media:NBM1381:gra001"></mediaResource><mediaResource alt="thumbnail image" rendition="webLoRes" mimeType="image/gif" href=""></mediaResource><mediaResource alt="original image" rendition="webOriginal" mimeType="image/jpeg" href=""></mediaResource><mediaResource alt="magnified image" rendition="webHiRes" mimeType="image/jpeg" href=""></mediaResource></mediaResourceGroup></blockFixed></p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>DTI IN THE 6OHDA RAT MODEL OF PARKINSON'S DISEASE</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract</title></titleInfo><name type="personal"><namePart type="given">Nadja</namePart><namePart type="family">Van Camp</namePart><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><affiliation>CEA, Institut d'Imagerie BioMédicale, Service Hospitalier Frédéric Joliot, Orsay, France</affiliation><affiliation>INSERM, U803, Orsay, France</affiliation><description>Correspondence: Bio‐Imaging Laboratory, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ines</namePart><namePart type="family">Blockx</namePart><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marleen</namePart><namePart type="family">Verhoye</namePart><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Cindy</namePart><namePart type="family">Casteels</namePart><affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frea</namePart><namePart type="family">Coun</namePart><affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alexander</namePart><namePart type="family">Leemans</namePart><affiliation>Cardiff University Brain Research Imaging Centre (CUBRIC), School of Psychology, Cardiff University, Cardiff, UK</affiliation><affiliation>Image Sciences Institute, Dept. Radiology, University Medical Center Utrecht, Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jan</namePart><namePart type="family">Sijbers</namePart><affiliation>Vision Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Veerle</namePart><namePart type="family">Baekelandt</namePart><affiliation>Laboratory for Neurobiology and Gene Therapy, Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Koen</namePart><namePart type="family">Van Laere</namePart><affiliation>Division of Nuclear Medicine, University Hospital Gasthuisberg and K.U. Leuven, Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Annemie</namePart><namePart type="family">Van der Linden</namePart><affiliation>Bio‐Imaging Laboratory, University of Antwerp, Antwerp, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Ltd.</publisher><place><placeTerm type="text">Chichester, UK</placeTerm></place><dateIssued encoding="w3cdtf">2009-08</dateIssued><dateCaptured encoding="w3cdtf">2008-09-22</dateCaptured><dateValid encoding="w3cdtf">2009-02-03</dateValid><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">2</extent><extent unit="references">45</extent></physicalDescription><abstract lang="en">Parkinson's disease (PD) is characterised by degeneration of the nigrostrial connection causing dramatic changes in the dopaminergic pathway underlying clinical pathology. Till now, no MRI tools were available to follow up any specific PD‐related neurodegeneration. However, recently, diffusion tensor imaging (DTI) has received considerable attention as a new and potential in vivo diagnostic tool for various neurodegenerative diseases. To assess this in PD, we performed DTI in the acute 6‐hydroxydopamine (6‐OHDA) rat model of PD to evaluate diffusion properties in the degenerating nigrostriatal pathway and its connecting structures. Injection of a neurotoxin in the striatum causes retrograde neurodegeneration of the nigrostriatal tract, and selective degeneration of nigral neurons. The advantage of this model is that the lesion size is well controllable by the injected dose of the toxin. The degree of functional impairment was evaluated in vivo using the amphetamine rotation test and µPET imaging of the dopamine transporter (DAT). Despite a nearly complete lesion of the nigrostriatal tract, DTI changes were limited to the ipsilateral substantia nigra (SN). In this study we demonstrate, using voxel‐based statistics (VBS), an increase in fractional anisotropy (FA), whereas all eigenvalues were significantly decreased. VBS enabled us to visualise neurodegeneration of a cluster of neurons but failed to detect degeneration of more diffuse microstructures such as the nigrostriatal fibres or the dopaminergic endings in the striatum. VBS without a priori information proved to be better than manual segmentation of brain structures as it does not suffer from volume averaging and is not susceptible to erroneous segmentations of brain regions that show very little contrast on MRI images such as SN. Copyright © 2009 John Wiley & Sons, Ltd.</abstract><abstract type="graphical" lang="en">DTI was performed in the 6‐hydroxydopamine rat model of Parkinson's disease to evaluate the degeneration of the nigrostriatal pathway. We demonstrated a significant increase in fractional anisotropy, and decrease of all eigenvalues in the SN. Voxel‐based statistics proved to be better than manual segmentation of brain structures as they do not suffer from volume averaging and are not susceptible to erroneous segmentation of brain regions.</abstract><note type="funding">The EC‐FP6‐project DiMI - No. LSHB‐CT‐2005‐512146; </note><note type="funding">The EC‐FP6‐project EMIL - No. LSHC‐CT‐2004‐503569; </note><note type="funding">The Institute for the Promotion of Innovation by Science and Technology in Flanders - No. IWT SBO/030238; </note><note type="funding">The European Community RATstreamTM STREP - No. LSHM‐CT‐2007‐037846; </note><note type="funding">Inter University Attraction Poles - No. IUAP‐NIMI‐P6/38; </note><subject lang="en"><genre>Keywords</genre><topic>DTI</topic><topic>Parkinson's disease</topic><topic>6‐OHDA rat model</topic><topic>neurodegeneration</topic></subject><relatedItem type="host"><titleInfo><title>NMR in Biomedicine</title><subTitle>An International Journal Devoted to the Development and Application of Magnetic Resonance In vivo</subTitle></titleInfo><titleInfo type="abbreviated"><title>NMR Biomed.</title></titleInfo><genre type="Journal">journal</genre><subject><genre>article category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0952-3480</identifier><identifier type="eISSN">1099-1492</identifier><identifier type="DOI">10.1002/(ISSN)1099-1492</identifier><identifier type="PublisherID">NBM</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>22</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>697</start><end>706</end><total>10</total></extent></part></relatedItem><identifier type="istex">E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA</identifier><identifier type="DOI">10.1002/nbm.1381</identifier><identifier type="ArticleID">NBM1381</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2009 John Wiley & Sons, Ltd.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Ltd.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D04 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000D04 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= ISTEX:E5C66EB71369FC9AE2C30D95DBA381175DB6E0FA
|texte= Diffusion tensor imaging in a rat model of Parkinson's disease after lesioning of the nigrostriatal tract
}}
| This area was generated with Dilib version V0.6.23. |  |