High and low affinity [3H]imipramine binding sites in control and Parkinsonian brains
Identifieur interne : 000319 ( France/Analysis ); précédent : 000318; suivant : 000320High and low affinity [3H]imipramine binding sites in control and Parkinsonian brains
Auteurs : Roland Cash [France] ; Rita Raisman [France] ; Alain Ploska [France] ; Yves Agid [France]Source :
- European Journal of Pharmacology [ 0014-2999 ] ; 1985.
Abstract
[3H]Imipramine binding was studied in the prefrontal cortex and putamen of post-mortem brains from control and Parkinsonian subjects. Saturation and inhibition curves showed both high affinity [3H]imipramine binding related to the serotonin uptake mechanism and low affinity binding which was sodium-independent and unrelated to serotonergic uptake. After subcellular fractionation, high affinity [3H]imipramine binding sites were enriched in synaptosomal fractions. In Parkinson's disease, where brain serotonin concentrations are decreased, there was a significant reduction in the density of the high affinity binding in the prefrontal cortex and putamen while the characteristics of the low affinity binding sites remained unchanged. After subcellular fractionation of the putamen of Parkinsonian patients, the decrease in [3H]imipramine binding was found predominantly in the synaptosomal fractions. These results are consistent with a relation between the high affinity [3H]imipramine binding sites and the neuronal serotonin uptake mechanism. Estimation of [3H]imipramine binding could be used as a specific marker for the study of serotonergic innervation in human post-mortem material. The reduction in the density of tricylic antidepressant binding sites found in cortical and subcortical areas of Parkinsonian brains may be somehow implicated in the depression often seen in patients.
Url:
DOI: 10.1016/0014-2999(85)90473-X
Affiliations:
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ISTEX:554B347D4A0B3A43C3212524C4A1C89BAE23F15FLe document en format XML
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Saturation and inhibition curves showed both high affinity [3H]imipramine binding related to the serotonin uptake mechanism and low affinity binding which was sodium-independent and unrelated to serotonergic uptake. After subcellular fractionation, high affinity [3H]imipramine binding sites were enriched in synaptosomal fractions. In Parkinson's disease, where brain serotonin concentrations are decreased, there was a significant reduction in the density of the high affinity binding in the prefrontal cortex and putamen while the characteristics of the low affinity binding sites remained unchanged. After subcellular fractionation of the putamen of Parkinsonian patients, the decrease in [3H]imipramine binding was found predominantly in the synaptosomal fractions. These results are consistent with a relation between the high affinity [3H]imipramine binding sites and the neuronal serotonin uptake mechanism. Estimation of [3H]imipramine binding could be used as a specific marker for the study of serotonergic innervation in human post-mortem material. The reduction in the density of tricylic antidepressant binding sites found in cortical and subcortical areas of Parkinsonian brains may be somehow implicated in the depression often seen in patients.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li><li>Paris Cédex 13</li></settlement><orgName><li>Hôpital de la Salpêtrière</li></orgName></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Cash, Roland" sort="Cash, Roland" uniqKey="Cash R" first="Roland" last="Cash">Roland Cash</name></region><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name><name sortKey="Ploska, Alain" sort="Ploska, Alain" uniqKey="Ploska A" first="Alain" last="Ploska">Alain Ploska</name><name sortKey="Raisman, Rita" sort="Raisman, Rita" uniqKey="Raisman R" first="Rita" last="Raisman">Rita Raisman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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