In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones.
Identifieur interne : 000242 ( PubMed/Curation ); précédent : 000241; suivant : 000243In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones.
Auteurs : Laure-Estelle Cassagnes [France] ; Pierre Perio [France] ; Gilles Ferry [France] ; Natacha Moulharat [France] ; Mathias Antoine [France] ; Régis Gayon [France] ; Jean A. Boutin [France] ; Françoise Nepveu [France] ; Karine Reybier [France]Source :
- Free radical biology & medicine [ 1873-4596 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Benzoquinones, Free Radicals, Quinone Reductases.
- chemical , metabolism : Oxygen, Quinone Reductases, Reactive Oxygen Species.
- Animals, CHO Cells, Cricetinae, Cricetulus, Electron Spin Resonance Spectroscopy, Oxidation-Reduction.
Abstract
Quinones are highly reactive molecules that readily undergo either one- or two-electron reduction. One-electron reduction of quinones or their derivatives by enzymes such as cytochrome P450 reductase or other flavoproteins generates unstable semiquinones, which undergo redox cycling in the presence of molecular oxygen leading to the formation of highly reactive oxygen species. Quinone reductases 1 and 2 (QR1 and QR2) catalyze the two-electron reduction of quinones to form hydroquinones, which can be removed from the cell by conjugation of the hydroxyl with glucuronide or sulfate thus avoiding its autoxidation and the formation of free radicals and highly reactive oxygen species. This characteristic confers a detoxifying enzyme role to QR1 and QR2, even if this character is strongly linked to the excretion capacity of the cell. Using EPR spectroscopy and confocal microscopy we demonstrated that the amount of reactive oxygen species (ROS) produced by Chinese hamster ovary (CHO) cells overexpressing QR1 or QR2 compared to naive CHO cells was determined by the quinone structural type. Indeed, whereas the amount of ROS produced in the cell was strongly decreased with para-quinones such as menadione in the presence of quinone reductase 1 or 2, a strong increase in ROS was recorded with ortho-quinones such as adrenochrome, aminochrome, dopachrome, or 3,5-di-tert-butyl-o-benzoquinone in cells overexpressing QR, especially QR2. These differences could originate from the excretion process, which is different for para- and ortho-quinones. These results are of particular interest in the case of dopamine considering the association of QR2 with various neurological disorders such as Parkinson disease.
DOI: 10.1016/j.freeradbiomed.2015.07.150
PubMed: 26386287
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Boutin</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine</wicri:regionArea></affiliation></author><author><name sortKey="Nepveu, Francoise" sort="Nepveu, Francoise" uniqKey="Nepveu F" first="Françoise" last="Nepveu">Françoise Nepveu</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author><author><name sortKey="Reybier, Karine" sort="Reybier, Karine" uniqKey="Reybier K" first="Karine" last="Reybier">Karine Reybier</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France. Electronic address: karine.reybier-vuattoux@univ-tlse3.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26386287</idno><idno type="pmid">26386287</idno><idno type="doi">10.1016/j.freeradbiomed.2015.07.150</idno><idno type="wicri:Area/PubMed/Corpus">000243</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000243</idno><idno type="wicri:Area/PubMed/Curation">000242</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000242</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones.</title><author><name sortKey="Cassagnes, Laure Estelle" sort="Cassagnes, Laure Estelle" uniqKey="Cassagnes L" first="Laure-Estelle" last="Cassagnes">Laure-Estelle Cassagnes</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author><author><name sortKey="Perio, Pierre" sort="Perio, Pierre" uniqKey="Perio P" first="Pierre" last="Perio">Pierre Perio</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author><author><name sortKey="Ferry, Gilles" sort="Ferry, Gilles" uniqKey="Ferry G" first="Gilles" last="Ferry">Gilles Ferry</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine</wicri:regionArea></affiliation></author><author><name sortKey="Moulharat, Natacha" sort="Moulharat, Natacha" uniqKey="Moulharat N" first="Natacha" last="Moulharat">Natacha Moulharat</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine</wicri:regionArea></affiliation></author><author><name sortKey="Antoine, Mathias" sort="Antoine, Mathias" uniqKey="Antoine M" first="Mathias" last="Antoine">Mathias Antoine</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine</wicri:regionArea></affiliation></author><author><name sortKey="Gayon, Regis" sort="Gayon, Regis" uniqKey="Gayon R" first="Régis" last="Gayon">Régis Gayon</name><affiliation wicri:level="1"><nlm:affiliation>Vectalys SAS, Canal Biotech 2, 31400 Toulouse, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Vectalys SAS, Canal Biotech 2, 31400 Toulouse</wicri:regionArea></affiliation></author><author><name sortKey="Boutin, Jean A" sort="Boutin, Jean A" uniqKey="Boutin J" first="Jean A" last="Boutin">Jean A. Boutin</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine</wicri:regionArea></affiliation></author><author><name sortKey="Nepveu, Francoise" sort="Nepveu, Francoise" uniqKey="Nepveu F" first="Françoise" last="Nepveu">Françoise Nepveu</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author><author><name sortKey="Reybier, Karine" sort="Reybier, Karine" uniqKey="Reybier K" first="Karine" last="Reybier">Karine Reybier</name><affiliation wicri:level="1"><nlm:affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France. Electronic address: karine.reybier-vuattoux@univ-tlse3.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9</wicri:regionArea></affiliation></author></analytic><series><title level="j">Free radical biology & medicine</title><idno type="eISSN">1873-4596</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Benzoquinones (chemistry)</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Electron Spin Resonance Spectroscopy</term><term>Free Radicals (chemistry)</term><term>Oxidation-Reduction</term><term>Oxygen (metabolism)</term><term>Quinone Reductases (chemistry)</term><term>Quinone Reductases (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Benzoquinones</term><term>Free Radicals</term><term>Quinone Reductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oxygen</term><term>Quinone Reductases</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetinae</term><term>Cricetulus</term><term>Electron Spin Resonance Spectroscopy</term><term>Oxidation-Reduction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Quinones are highly reactive molecules that readily undergo either one- or two-electron reduction. One-electron reduction of quinones or their derivatives by enzymes such as cytochrome P450 reductase or other flavoproteins generates unstable semiquinones, which undergo redox cycling in the presence of molecular oxygen leading to the formation of highly reactive oxygen species. Quinone reductases 1 and 2 (QR1 and QR2) catalyze the two-electron reduction of quinones to form hydroquinones, which can be removed from the cell by conjugation of the hydroxyl with glucuronide or sulfate thus avoiding its autoxidation and the formation of free radicals and highly reactive oxygen species. This characteristic confers a detoxifying enzyme role to QR1 and QR2, even if this character is strongly linked to the excretion capacity of the cell. Using EPR spectroscopy and confocal microscopy we demonstrated that the amount of reactive oxygen species (ROS) produced by Chinese hamster ovary (CHO) cells overexpressing QR1 or QR2 compared to naive CHO cells was determined by the quinone structural type. Indeed, whereas the amount of ROS produced in the cell was strongly decreased with para-quinones such as menadione in the presence of quinone reductase 1 or 2, a strong increase in ROS was recorded with ortho-quinones such as adrenochrome, aminochrome, dopachrome, or 3,5-di-tert-butyl-o-benzoquinone in cells overexpressing QR, especially QR2. These differences could originate from the excretion process, which is different for para- and ortho-quinones. These results are of particular interest in the case of dopamine considering the association of QR2 with various neurological disorders such as Parkinson disease.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26386287</PMID><DateCreated><Year>2015</Year><Month>12</Month><Day>18</Day></DateCreated><DateCompleted><Year>2016</Year><Month>09</Month><Day>22</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>26</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4596</ISSN><JournalIssue CitedMedium="Internet"><Volume>89</Volume><PubDate><Year>2015</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Free radical biology & medicine</Title><ISOAbbreviation>Free Radic. Biol. Med.</ISOAbbreviation></Journal><ArticleTitle>In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones.</ArticleTitle><Pagination><MedlinePgn>126-34</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.freeradbiomed.2015.07.150</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0891-5849(15)00510-9</ELocationID><Abstract><AbstractText>Quinones are highly reactive molecules that readily undergo either one- or two-electron reduction. One-electron reduction of quinones or their derivatives by enzymes such as cytochrome P450 reductase or other flavoproteins generates unstable semiquinones, which undergo redox cycling in the presence of molecular oxygen leading to the formation of highly reactive oxygen species. Quinone reductases 1 and 2 (QR1 and QR2) catalyze the two-electron reduction of quinones to form hydroquinones, which can be removed from the cell by conjugation of the hydroxyl with glucuronide or sulfate thus avoiding its autoxidation and the formation of free radicals and highly reactive oxygen species. This characteristic confers a detoxifying enzyme role to QR1 and QR2, even if this character is strongly linked to the excretion capacity of the cell. Using EPR spectroscopy and confocal microscopy we demonstrated that the amount of reactive oxygen species (ROS) produced by Chinese hamster ovary (CHO) cells overexpressing QR1 or QR2 compared to naive CHO cells was determined by the quinone structural type. Indeed, whereas the amount of ROS produced in the cell was strongly decreased with para-quinones such as menadione in the presence of quinone reductase 1 or 2, a strong increase in ROS was recorded with ortho-quinones such as adrenochrome, aminochrome, dopachrome, or 3,5-di-tert-butyl-o-benzoquinone in cells overexpressing QR, especially QR2. These differences could originate from the excretion process, which is different for para- and ortho-quinones. These results are of particular interest in the case of dopamine considering the association of QR2 with various neurological disorders such as Parkinson disease.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cassagnes</LastName><ForeName>Laure-Estelle</ForeName><Initials>LE</Initials><AffiliationInfo><Affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Perio</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferry</LastName><ForeName>Gilles</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moulharat</LastName><ForeName>Natacha</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Antoine</LastName><ForeName>Mathias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gayon</LastName><ForeName>Régis</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Vectalys SAS, Canal Biotech 2, 31400 Toulouse, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boutin</LastName><ForeName>Jean A</ForeName><Initials>JA</Initials><AffiliationInfo><Affiliation>Biotechnologie, Pharmacologie Moléculaire et Cellulaire, Institut de Recherches Servier, 78290 Croissy sur Seine, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nepveu</LastName><ForeName>Françoise</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reybier</LastName><ForeName>Karine</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Université de Toulouse, UPS, UMR 152 PHARMA-DEV, F-31062 Toulouse Cedex 9, France; IRD, UMR 152, F-31062 Toulouse Cedex 9, France. Electronic address: karine.reybier-vuattoux@univ-tlse3.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>09</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Free Radic Biol Med</MedlineTA><NlmUniqueID>8709159</NlmUniqueID><ISSNLinking>0891-5849</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016227">Benzoquinones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005609">Free Radicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>3T006GV98U</RegistryNumber><NameOfSubstance UI="C004532">quinone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.6.99.-</RegistryNumber><NameOfSubstance UI="D011808">Quinone Reductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016227" MajorTopicYN="N">Benzoquinones</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016466" MajorTopicYN="N">CHO Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006224" MajorTopicYN="N">Cricetinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003412" MajorTopicYN="N">Cricetulus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004578" MajorTopicYN="N">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005609" MajorTopicYN="N">Free Radicals</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010100" MajorTopicYN="N">Oxygen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011808" MajorTopicYN="N">Quinone Reductases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">EPR</Keyword><Keyword MajorTopicYN="N">Free radicals</Keyword><Keyword MajorTopicYN="N">NQO1</Keyword><Keyword MajorTopicYN="N">NQO2</Keyword><Keyword MajorTopicYN="N">Ortho-quinones</Keyword><Keyword MajorTopicYN="N">Oxidative stress</Keyword><Keyword MajorTopicYN="N">Para-quinones</Keyword><Keyword MajorTopicYN="N">Quinone reductase</Keyword><Keyword MajorTopicYN="N">Reactive oxygen species</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>06</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26386287</ArticleId><ArticleId IdType="pii">S0891-5849(15)00510-9</ArticleId><ArticleId IdType="doi">10.1016/j.freeradbiomed.2015.07.150</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones.
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