The C289G and C418R missense mutations cause rapid sequestration of human Parkin into insoluble aggregates.
Identifieur interne : 001065 ( PubMed/Corpus ); précédent : 001064; suivant : 001066The C289G and C418R missense mutations cause rapid sequestration of human Parkin into insoluble aggregates.
Auteurs : Wen-Jie Gu ; Olga Corti ; Francisco Araujo ; Cornelia Hampe ; Sandrine Jacquier ; Christoph B. Lücking ; Nacer Abbas ; Charles Duyckaerts ; Thomas Rooney ; Laurent Pradier ; Merle Ruberg ; Alexis BriceSource :
- Neurobiology of disease [ 0969-9961 ] ; 2003.
English descriptors
- KwdEn :
- Animals, COS Cells, Cysteine Endopeptidases (metabolism), Humans, Inclusion Bodies (genetics), Inclusion Bodies (metabolism), Macromolecular Substances, Microtubules (metabolism), Multienzyme Complexes (metabolism), Mutation, Missense (genetics), Neurons (metabolism), Neurons (pathology), Parkinson Disease (genetics), Parkinson Disease (metabolism), Parkinson Disease (physiopathology), Proteasome Endopeptidase Complex, Protein Folding, Protein Structure, Tertiary (genetics), Protein Transport (physiology), Solubility, Ubiquitin-Protein Ligases (genetics), Ubiquitin-Protein Ligases (metabolism), Ubiquitins (metabolism).
- MESH :
- chemical , genetics : Ubiquitin-Protein Ligases.
- chemical , metabolism : Cysteine Endopeptidases, Multienzyme Complexes, Ubiquitin-Protein Ligases, Ubiquitins.
- genetics : Inclusion Bodies, Mutation, Missense, Parkinson Disease, Protein Structure, Tertiary.
- metabolism : Inclusion Bodies, Microtubules, Neurons, Parkinson Disease.
- pathology : Neurons.
- physiology : Protein Transport.
- physiopathology : Parkinson Disease.
- Animals, COS Cells, Humans, Macromolecular Substances, Proteasome Endopeptidase Complex, Protein Folding, Solubility.
Abstract
Mutations in the parkin gene are responsible for autosomal recessive parkinsonism. The disease-linked missense mutations are highly concentrated in the RING-IBR-RING domains of Parkin. In this study, we investigated the consequences of several missense parkin gene mutations in cell culture. We have demonstrated that two of these mutations (C289G and C418R), which replace consensus cysteine residues in the RING domains, significantly decrease the solubility of Parkin in cells. Upon overexpression, the presumably misfolded proteins formed cytoplasmic aggregates that concentrated into large perinuclear inclusion bodies when proteasome activity was inhibited. This process required active microtubule-dependent retrograde transport, as previously reported for aggresome formation. These results provide information on the molecular basis of the loss of function caused by mutations of critical residues in Parkin. They also contribute to our understanding of the cellular mechanism underlying the aggregation of mutant Parkin.
PubMed: 14678753
Links to Exploration step
pubmed:14678753Le document en format XML
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<author><name sortKey="Corti, Olga" sort="Corti, Olga" uniqKey="Corti O" first="Olga" last="Corti">Olga Corti</name>
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<author><name sortKey="Araujo, Francisco" sort="Araujo, Francisco" uniqKey="Araujo F" first="Francisco" last="Araujo">Francisco Araujo</name>
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<author><name sortKey="Hampe, Cornelia" sort="Hampe, Cornelia" uniqKey="Hampe C" first="Cornelia" last="Hampe">Cornelia Hampe</name>
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<author><name sortKey="Jacquier, Sandrine" sort="Jacquier, Sandrine" uniqKey="Jacquier S" first="Sandrine" last="Jacquier">Sandrine Jacquier</name>
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<author><name sortKey="Lucking, Christoph B" sort="Lucking, Christoph B" uniqKey="Lucking C" first="Christoph B" last="Lücking">Christoph B. Lücking</name>
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<author><name sortKey="Abbas, Nacer" sort="Abbas, Nacer" uniqKey="Abbas N" first="Nacer" last="Abbas">Nacer Abbas</name>
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<author><name sortKey="Duyckaerts, Charles" sort="Duyckaerts, Charles" uniqKey="Duyckaerts C" first="Charles" last="Duyckaerts">Charles Duyckaerts</name>
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<series><title level="j">Neurobiology of disease</title>
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<term>COS Cells</term>
<term>Cysteine Endopeptidases (metabolism)</term>
<term>Humans</term>
<term>Inclusion Bodies (genetics)</term>
<term>Inclusion Bodies (metabolism)</term>
<term>Macromolecular Substances</term>
<term>Microtubules (metabolism)</term>
<term>Multienzyme Complexes (metabolism)</term>
<term>Mutation, Missense (genetics)</term>
<term>Neurons (metabolism)</term>
<term>Neurons (pathology)</term>
<term>Parkinson Disease (genetics)</term>
<term>Parkinson Disease (metabolism)</term>
<term>Parkinson Disease (physiopathology)</term>
<term>Proteasome Endopeptidase Complex</term>
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<term>Protein Structure, Tertiary (genetics)</term>
<term>Protein Transport (physiology)</term>
<term>Solubility</term>
<term>Ubiquitin-Protein Ligases (genetics)</term>
<term>Ubiquitin-Protein Ligases (metabolism)</term>
<term>Ubiquitins (metabolism)</term>
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<term>Ubiquitin-Protein Ligases</term>
<term>Ubiquitins</term>
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<term>Mutation, Missense</term>
<term>Parkinson Disease</term>
<term>Protein Structure, Tertiary</term>
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<term>Microtubules</term>
<term>Neurons</term>
<term>Parkinson Disease</term>
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<term>COS Cells</term>
<term>Humans</term>
<term>Macromolecular Substances</term>
<term>Proteasome Endopeptidase Complex</term>
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<front><div type="abstract" xml:lang="en">Mutations in the parkin gene are responsible for autosomal recessive parkinsonism. The disease-linked missense mutations are highly concentrated in the RING-IBR-RING domains of Parkin. In this study, we investigated the consequences of several missense parkin gene mutations in cell culture. We have demonstrated that two of these mutations (C289G and C418R), which replace consensus cysteine residues in the RING domains, significantly decrease the solubility of Parkin in cells. Upon overexpression, the presumably misfolded proteins formed cytoplasmic aggregates that concentrated into large perinuclear inclusion bodies when proteasome activity was inhibited. This process required active microtubule-dependent retrograde transport, as previously reported for aggresome formation. These results provide information on the molecular basis of the loss of function caused by mutations of critical residues in Parkin. They also contribute to our understanding of the cellular mechanism underlying the aggregation of mutant Parkin.</div>
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<Abstract><AbstractText>Mutations in the parkin gene are responsible for autosomal recessive parkinsonism. The disease-linked missense mutations are highly concentrated in the RING-IBR-RING domains of Parkin. In this study, we investigated the consequences of several missense parkin gene mutations in cell culture. We have demonstrated that two of these mutations (C289G and C418R), which replace consensus cysteine residues in the RING domains, significantly decrease the solubility of Parkin in cells. Upon overexpression, the presumably misfolded proteins formed cytoplasmic aggregates that concentrated into large perinuclear inclusion bodies when proteasome activity was inhibited. This process required active microtubule-dependent retrograde transport, as previously reported for aggresome formation. These results provide information on the molecular basis of the loss of function caused by mutations of critical residues in Parkin. They also contribute to our understanding of the cellular mechanism underlying the aggregation of mutant Parkin.</AbstractText>
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