Detection of disease-associated α-synuclein in the cerebrospinal fluid: a feasibility study.
Identifieur interne : 000513 ( PubMed/Corpus ); précédent : 000512; suivant : 000514Detection of disease-associated α-synuclein in the cerebrospinal fluid: a feasibility study.
Auteurs : Ursula Unterberger ; Ingolf Lachmann ; Till Voigtl Nder ; Walter Pirker ; Anna S. Berghoff ; Katharina Flach ; Uta Wagner ; Aline Geneste ; Armand Perret-Liaudet ; Gabor G. KovacsSource :
- Clinical neuropathology [ 0722-5091 ]
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Alzheimer Disease (cerebrospinal fluid), Alzheimer Disease (diagnosis), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Feasibility Studies, Female, Humans, Immunomagnetic Separation (methods), Immunomagnetic Separation (standards), Lewy Body Disease (cerebrospinal fluid), Lewy Body Disease (diagnosis), Male, Middle Aged, Neurodegenerative Diseases (cerebrospinal fluid), Neurodegenerative Diseases (diagnosis), Parkinson Disease (cerebrospinal fluid), Parkinson Disease (diagnosis), Reproducibility of Results, alpha-Synuclein (cerebrospinal fluid).
- MESH :
- chemical , cerebrospinal fluid : alpha-Synuclein.
- cerebrospinal fluid : Alzheimer Disease, Lewy Body Disease, Neurodegenerative Diseases, Parkinson Disease.
- diagnosis : Alzheimer Disease, Lewy Body Disease, Neurodegenerative Diseases, Parkinson Disease.
- methods : Enzyme-Linked Immunosorbent Assay, Immunomagnetic Separation.
- standards : Enzyme-Linked Immunosorbent Assay, Immunomagnetic Separation.
- Adult, Aged, Aged, 80 and over, Feasibility Studies, Female, Humans, Male, Middle Aged, Reproducibility of Results.
Abstract
With the aim to evaluate the significance and reliability of detecting disease-specific α-synuclein in the cerebrospinal fluid (CSF) we developed an ELISA and bead-assay. We used a commercial antibody (5G4) that does not bind to the physiological monomeric form of α-synuclein, but is highly specific for the disease-associated forms, including high molecular weight fraction of β-sheet rich oligomers. We applied both tests in CSF from a series of neuropathologically confirmed α-synucleinopathy cases, including Parkinson' disease dementia (PDD) and dementia with Lewy bodies (DLB) (n = 7), as well as Alzheimer' disease (n = 6), and control patients without neurodegenerative pathologies (n = 9). Disease-specific α-synuclein was detectable in the CSF in a subset of patients with α-synuclein pathology in the brain. When combined with the analysis of total α-synuclein, the bead-assay for disease-specific α-synuclein was highly specific for PDD/DLB. Detection of disease-associated αsynuclein combined with the total levels of α-synuclein is a promising tool for the in-vivo diagnosis of α-synucleinopathies, including PDD and LBD.
PubMed: 25131945
Links to Exploration step
pubmed:25131945Le document en format XML
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<front><div type="abstract" xml:lang="en">With the aim to evaluate the significance and reliability of detecting disease-specific α-synuclein in the cerebrospinal fluid (CSF) we developed an ELISA and bead-assay. We used a commercial antibody (5G4) that does not bind to the physiological monomeric form of α-synuclein, but is highly specific for the disease-associated forms, including high molecular weight fraction of β-sheet rich oligomers. We applied both tests in CSF from a series of neuropathologically confirmed α-synucleinopathy cases, including Parkinson' disease dementia (PDD) and dementia with Lewy bodies (DLB) (n = 7), as well as Alzheimer' disease (n = 6), and control patients without neurodegenerative pathologies (n = 9). Disease-specific α-synuclein was detectable in the CSF in a subset of patients with α-synuclein pathology in the brain. When combined with the analysis of total α-synuclein, the bead-assay for disease-specific α-synuclein was highly specific for PDD/DLB. Detection of disease-associated αsynuclein combined with the total levels of α-synuclein is a promising tool for the in-vivo diagnosis of α-synucleinopathies, including PDD and LBD.</div>
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<Abstract><AbstractText>With the aim to evaluate the significance and reliability of detecting disease-specific α-synuclein in the cerebrospinal fluid (CSF) we developed an ELISA and bead-assay. We used a commercial antibody (5G4) that does not bind to the physiological monomeric form of α-synuclein, but is highly specific for the disease-associated forms, including high molecular weight fraction of β-sheet rich oligomers. We applied both tests in CSF from a series of neuropathologically confirmed α-synucleinopathy cases, including Parkinson' disease dementia (PDD) and dementia with Lewy bodies (DLB) (n = 7), as well as Alzheimer' disease (n = 6), and control patients without neurodegenerative pathologies (n = 9). Disease-specific α-synuclein was detectable in the CSF in a subset of patients with α-synuclein pathology in the brain. When combined with the analysis of total α-synuclein, the bead-assay for disease-specific α-synuclein was highly specific for PDD/DLB. Detection of disease-associated αsynuclein combined with the total levels of α-synuclein is a promising tool for the in-vivo diagnosis of α-synucleinopathies, including PDD and LBD.</AbstractText>
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