α-Synuclein is a Novel Microtubule Dynamase.
Identifieur interne : 000087 ( PubMed/Corpus ); précédent : 000086; suivant : 000088α-Synuclein is a Novel Microtubule Dynamase.
Auteurs : Daniele Cartelli ; Alessandro Aliverti ; Alberto Barbiroli ; Carlo Santambrogio ; Enzio M. Ragg ; Francesca V M. Casagrande ; Francesca Cantele ; Silvia Beltramone ; Jacopo Marangon ; Carmelita De Gregorio ; Vittorio Pandini ; Marco Emanuele ; Evelina Chieregatti ; Stefano Pieraccini ; Staffan Holmqvist ; Luigi Bubacco ; Laurent Roybon ; Gianni Pezzoli ; Rita Grandori ; Isabelle Arnal ; Graziella CappellettiSource :
- Scientific reports [ 2045-2322 ] ; 2016.
Abstract
α-Synuclein is a presynaptic protein associated to Parkinson's disease, which is unstructured when free in the cytoplasm and adopts α helical conformation when bound to vesicles. After decades of intense studies, α-Synuclein physiology is still difficult to clear up due to its interaction with multiple partners and its involvement in a pletora of neuronal functions. Here, we looked at the remarkably neglected interplay between α-Synuclein and microtubules, which potentially impacts on synaptic functionality. In order to identify the mechanisms underlying these actions, we investigated the interaction between purified α-Synuclein and tubulin. We demonstrated that α-Synuclein binds to microtubules and tubulin α2β2 tetramer; the latter interaction inducing the formation of helical segment(s) in the α-Synuclein polypeptide. This structural change seems to enable α-Synuclein to promote microtubule nucleation and to enhance microtubule growth rate and catastrophe frequency, both in vitro and in cell. We also showed that Parkinson's disease-linked α-Synuclein variants do not undergo tubulin-induced folding and cause tubulin aggregation rather than polymerization. Our data enable us to propose α-Synuclein as a novel, foldable, microtubule-dynamase, which influences microtubule organisation through its binding to tubulin and its regulating effects on microtubule nucleation and dynamics.
DOI: 10.1038/srep33289
PubMed: 27628239
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Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Barbiroli, Alberto" sort="Barbiroli, Alberto" uniqKey="Barbiroli A" first="Alberto" last="Barbiroli">Alberto Barbiroli</name><affiliation><nlm:affiliation>Dept. of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Santambrogio, Carlo" sort="Santambrogio, Carlo" uniqKey="Santambrogio C" first="Carlo" last="Santambrogio">Carlo Santambrogio</name><affiliation><nlm:affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Ragg, Enzio M" sort="Ragg, Enzio M" uniqKey="Ragg E" first="Enzio M" last="Ragg">Enzio M. 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Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Emanuele, Marco" sort="Emanuele, Marco" uniqKey="Emanuele M" first="Marco" last="Emanuele">Marco Emanuele</name><affiliation><nlm:affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Chieregatti, Evelina" sort="Chieregatti, Evelina" uniqKey="Chieregatti E" first="Evelina" last="Chieregatti">Evelina Chieregatti</name><affiliation><nlm:affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Pieraccini, Stefano" sort="Pieraccini, Stefano" uniqKey="Pieraccini S" first="Stefano" last="Pieraccini">Stefano Pieraccini</name><affiliation><nlm:affiliation>Dept. Chemistry, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Holmqvist, Staffan" sort="Holmqvist, Staffan" uniqKey="Holmqvist S" first="Staffan" last="Holmqvist">Staffan Holmqvist</name><affiliation><nlm:affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Bubacco, Luigi" sort="Bubacco, Luigi" uniqKey="Bubacco L" first="Luigi" last="Bubacco">Luigi Bubacco</name><affiliation><nlm:affiliation>Dept. Biology, University of Padova, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Roybon, Laurent" sort="Roybon, Laurent" uniqKey="Roybon L" first="Laurent" last="Roybon">Laurent Roybon</name><affiliation><nlm:affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Pezzoli, Gianni" sort="Pezzoli, Gianni" uniqKey="Pezzoli G" first="Gianni" last="Pezzoli">Gianni Pezzoli</name><affiliation><nlm:affiliation>Parkinson Institute, ASST G. Pini-CTO, ex ICP, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Grandori, Rita" sort="Grandori, Rita" uniqKey="Grandori R" first="Rita" last="Grandori">Rita Grandori</name><affiliation><nlm:affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Arnal, Isabelle" sort="Arnal, Isabelle" uniqKey="Arnal I" first="Isabelle" last="Arnal">Isabelle Arnal</name><affiliation><nlm:affiliation>Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cappelletti, Graziella" sort="Cappelletti, Graziella" uniqKey="Cappelletti G" first="Graziella" last="Cappelletti">Graziella Cappelletti</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27628239</idno><idno type="pmid">27628239</idno><idno type="doi">10.1038/srep33289</idno><idno type="wicri:Area/PubMed/Corpus">000087</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000087</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">α-Synuclein is a Novel Microtubule Dynamase.</title><author><name sortKey="Cartelli, Daniele" sort="Cartelli, Daniele" uniqKey="Cartelli D" first="Daniele" last="Cartelli">Daniele Cartelli</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Aliverti, Alessandro" sort="Aliverti, Alessandro" uniqKey="Aliverti A" first="Alessandro" last="Aliverti">Alessandro Aliverti</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Barbiroli, Alberto" sort="Barbiroli, Alberto" uniqKey="Barbiroli A" first="Alberto" last="Barbiroli">Alberto Barbiroli</name><affiliation><nlm:affiliation>Dept. of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Santambrogio, Carlo" sort="Santambrogio, Carlo" uniqKey="Santambrogio C" first="Carlo" last="Santambrogio">Carlo Santambrogio</name><affiliation><nlm:affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Ragg, Enzio M" sort="Ragg, Enzio M" uniqKey="Ragg E" first="Enzio M" last="Ragg">Enzio M. Ragg</name><affiliation><nlm:affiliation>Dept. of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Casagrande, Francesca V M" sort="Casagrande, Francesca V M" uniqKey="Casagrande F" first="Francesca V M" last="Casagrande">Francesca V M. Casagrande</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Cantele, Francesca" sort="Cantele, Francesca" uniqKey="Cantele F" first="Francesca" last="Cantele">Francesca Cantele</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Beltramone, Silvia" sort="Beltramone, Silvia" uniqKey="Beltramone S" first="Silvia" last="Beltramone">Silvia Beltramone</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Marangon, Jacopo" sort="Marangon, Jacopo" uniqKey="Marangon J" first="Jacopo" last="Marangon">Jacopo Marangon</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="De Gregorio, Carmelita" sort="De Gregorio, Carmelita" uniqKey="De Gregorio C" first="Carmelita" last="De Gregorio">Carmelita De Gregorio</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Pandini, Vittorio" sort="Pandini, Vittorio" uniqKey="Pandini V" first="Vittorio" last="Pandini">Vittorio Pandini</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Emanuele, Marco" sort="Emanuele, Marco" uniqKey="Emanuele M" first="Marco" last="Emanuele">Marco Emanuele</name><affiliation><nlm:affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Chieregatti, Evelina" sort="Chieregatti, Evelina" uniqKey="Chieregatti E" first="Evelina" last="Chieregatti">Evelina Chieregatti</name><affiliation><nlm:affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Pieraccini, Stefano" sort="Pieraccini, Stefano" uniqKey="Pieraccini S" first="Stefano" last="Pieraccini">Stefano Pieraccini</name><affiliation><nlm:affiliation>Dept. Chemistry, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Holmqvist, Staffan" sort="Holmqvist, Staffan" uniqKey="Holmqvist S" first="Staffan" last="Holmqvist">Staffan Holmqvist</name><affiliation><nlm:affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Bubacco, Luigi" sort="Bubacco, Luigi" uniqKey="Bubacco L" first="Luigi" last="Bubacco">Luigi Bubacco</name><affiliation><nlm:affiliation>Dept. Biology, University of Padova, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Roybon, Laurent" sort="Roybon, Laurent" uniqKey="Roybon L" first="Laurent" last="Roybon">Laurent Roybon</name><affiliation><nlm:affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</nlm:affiliation></affiliation></author><author><name sortKey="Pezzoli, Gianni" sort="Pezzoli, Gianni" uniqKey="Pezzoli G" first="Gianni" last="Pezzoli">Gianni Pezzoli</name><affiliation><nlm:affiliation>Parkinson Institute, ASST G. Pini-CTO, ex ICP, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Grandori, Rita" sort="Grandori, Rita" uniqKey="Grandori R" first="Rita" last="Grandori">Rita Grandori</name><affiliation><nlm:affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Arnal, Isabelle" sort="Arnal, Isabelle" uniqKey="Arnal I" first="Isabelle" last="Arnal">Isabelle Arnal</name><affiliation><nlm:affiliation>Grenoble Institut des Neurosciences, Grenoble, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cappelletti, Graziella" sort="Cappelletti, Graziella" uniqKey="Cappelletti G" first="Graziella" last="Cappelletti">Graziella Cappelletti</name><affiliation><nlm:affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">α-Synuclein is a presynaptic protein associated to Parkinson's disease, which is unstructured when free in the cytoplasm and adopts α helical conformation when bound to vesicles. After decades of intense studies, α-Synuclein physiology is still difficult to clear up due to its interaction with multiple partners and its involvement in a pletora of neuronal functions. Here, we looked at the remarkably neglected interplay between α-Synuclein and microtubules, which potentially impacts on synaptic functionality. In order to identify the mechanisms underlying these actions, we investigated the interaction between purified α-Synuclein and tubulin. We demonstrated that α-Synuclein binds to microtubules and tubulin α2β2 tetramer; the latter interaction inducing the formation of helical segment(s) in the α-Synuclein polypeptide. This structural change seems to enable α-Synuclein to promote microtubule nucleation and to enhance microtubule growth rate and catastrophe frequency, both in vitro and in cell. We also showed that Parkinson's disease-linked α-Synuclein variants do not undergo tubulin-induced folding and cause tubulin aggregation rather than polymerization. Our data enable us to propose α-Synuclein as a novel, foldable, microtubule-dynamase, which influences microtubule organisation through its binding to tubulin and its regulating effects on microtubule nucleation and dynamics.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27628239</PMID><DateCreated><Year>2016</Year><Month>09</Month><Day>16</Day></DateCreated><DateRevised><Year>2016</Year><Month>09</Month><Day>16</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><PubDate><Year>2016</Year><Month>Sep</Month><Day>15</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>α-Synuclein is a Novel Microtubule Dynamase.</ArticleTitle><Pagination><MedlinePgn>33289</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/srep33289</ELocationID><Abstract><AbstractText>α-Synuclein is a presynaptic protein associated to Parkinson's disease, which is unstructured when free in the cytoplasm and adopts α helical conformation when bound to vesicles. After decades of intense studies, α-Synuclein physiology is still difficult to clear up due to its interaction with multiple partners and its involvement in a pletora of neuronal functions. Here, we looked at the remarkably neglected interplay between α-Synuclein and microtubules, which potentially impacts on synaptic functionality. In order to identify the mechanisms underlying these actions, we investigated the interaction between purified α-Synuclein and tubulin. We demonstrated that α-Synuclein binds to microtubules and tubulin α2β2 tetramer; the latter interaction inducing the formation of helical segment(s) in the α-Synuclein polypeptide. This structural change seems to enable α-Synuclein to promote microtubule nucleation and to enhance microtubule growth rate and catastrophe frequency, both in vitro and in cell. We also showed that Parkinson's disease-linked α-Synuclein variants do not undergo tubulin-induced folding and cause tubulin aggregation rather than polymerization. Our data enable us to propose α-Synuclein as a novel, foldable, microtubule-dynamase, which influences microtubule organisation through its binding to tubulin and its regulating effects on microtubule nucleation and dynamics.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cartelli</LastName><ForeName>Daniele</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aliverti</LastName><ForeName>Alessandro</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barbiroli</LastName><ForeName>Alberto</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dept. of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santambrogio</LastName><ForeName>Carlo</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragg</LastName><ForeName>Enzio M</ForeName><Initials>EM</Initials><AffiliationInfo><Affiliation>Dept. of Food, Environmental and Nutritional Sciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Casagrande</LastName><ForeName>Francesca V M</ForeName><Initials>FV</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cantele</LastName><ForeName>Francesca</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Beltramone</LastName><ForeName>Silvia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marangon</LastName><ForeName>Jacopo</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>De Gregorio</LastName><ForeName>Carmelita</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pandini</LastName><ForeName>Vittorio</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Emanuele</LastName><ForeName>Marco</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chieregatti</LastName><ForeName>Evelina</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Dept. Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pieraccini</LastName><ForeName>Stefano</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dept. Chemistry, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Holmqvist</LastName><ForeName>Staffan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Strategic Research Area MultiPark and Lund Stem Cell Center, Lund University, Lund, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bubacco</LastName><ForeName>Luigi</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Dept. Biology, University of Padova, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roybon</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Stem Cell laboratory for CNS Disease Modeling, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Strategic Research Area MultiPark and Lund Stem Cell Center, Lund University, Lund, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pezzoli</LastName><ForeName>Gianni</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Parkinson Institute, ASST G. Pini-CTO, ex ICP, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grandori</LastName><ForeName>Rita</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Dept. Biotecnology and Biosciences, Università degli Studi di Milano-Bicocca, Milano, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arnal</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Grenoble Institut des Neurosciences, Grenoble, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cappelletti</LastName><ForeName>Graziella</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Dept. Biosciences, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Center of Excellence on Neurodegenerative Diseases, Università degli Studi di Milano, Milano, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>04</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>08</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27628239</ArticleId><ArticleId IdType="pii">srep33289</ArticleId><ArticleId IdType="doi">10.1038/srep33289</ArticleId><ArticleId IdType="pmc">PMC5024109</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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