Posttransplant Lymphoproliferative Disorders in Neuronal Xenotransplanted Macaques.
Identifieur interne : 000000 ( PubMed/Corpus ); suivant : 000001Posttransplant Lymphoproliferative Disorders in Neuronal Xenotransplanted Macaques.
Auteurs : L. Cavicchioli ; S. Ferraresso ; S. Westmoreland ; S. Kaliyaperumal ; H. Knight ; C. Crossan ; L. Scobie ; A. Danesi ; M. Vadori ; D. Trez ; R Aron Badin ; P. Hantraye ; E. CozziSource :
- Veterinary pathology [ 1544-2217 ] ; 2017.
Abstract
Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of lymphoid proliferations that occur in the setting of depressed T-cell function due to immunosuppressive therapy used following solid organ transplantation, hematopoietic stem cell transplantation, and also xenotransplantation. In the present study, 28 immunosuppressed parkinsonian Macaca fascicularis were intracerebrally injected with wild-type or CTLA4-Ig transgenic porcine xenografts to identify a suitable strategy to enable long-term cell survival, maturation, and differentiation. Nine of 28 (32%) immunosuppressed primates developed masses compatible with PTLD, located mainly in the gastrointestinal tract and/or nasal cavity. The masses were classified as monomorphic PTLD according to the World Health Organization classification. Immunohistochemistry and polymerase chain reaction (PCR) analyses revealed that the PTLDs were associated with macaca lymphocryptovirus as confirmed by double-labeling immunohistochemistry for CD20 and Epstein-Barr nuclear antigen 2 (EBNA-2), where the viral protein was located within the CD20+ neoplastic B cells. In sera from 3 distinct phases of the experimental life of the primates, testing by quantitative PCR revealed a progression of the viral load that paralleled the PTLD progression and no evidence of zoonotic transmission of porcine lymphotropic herpesvirus through xenoneuronal grafts. These data suggest that monitoring the variation of macaca lymphocryptovirus DNA in primates could be used as a possible early diagnostic tool for PTLD progression, allowing preemptive treatment such as immunosuppression therapy reduction.
DOI: 10.1177/0300985816669407
PubMed: 27694423
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Westmoreland</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kaliyaperumal, S" sort="Kaliyaperumal, S" uniqKey="Kaliyaperumal S" first="S" last="Kaliyaperumal">S. Kaliyaperumal</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Knight, H" sort="Knight, H" uniqKey="Knight H" first="H" last="Knight">H. Knight</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Crossan, C" sort="Crossan, C" uniqKey="Crossan C" first="C" last="Crossan">C. Crossan</name><affiliation><nlm:affiliation>3 Caledonian University, Glasgow, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Scobie, L" sort="Scobie, L" uniqKey="Scobie L" first="L" last="Scobie">L. Scobie</name><affiliation><nlm:affiliation>3 Caledonian University, Glasgow, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Danesi, A" sort="Danesi, A" uniqKey="Danesi A" first="A" last="Danesi">A. Danesi</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Vadori, M" sort="Vadori, M" uniqKey="Vadori M" first="M" last="Vadori">M. Vadori</name><affiliation><nlm:affiliation>4 CORIT, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Trez, D" sort="Trez, D" uniqKey="Trez D" first="D" last="Trez">D. Trez</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Badin, R Aron" sort="Badin, R Aron" uniqKey="Badin R" first="R Aron" last="Badin">R Aron Badin</name><affiliation><nlm:affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hantraye, P" sort="Hantraye, P" uniqKey="Hantraye P" first="P" last="Hantraye">P. Hantraye</name><affiliation><nlm:affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cozzi, E" sort="Cozzi, E" uniqKey="Cozzi E" first="E" last="Cozzi">E. Cozzi</name><affiliation><nlm:affiliation>4 CORIT, Padova, Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:27694423</idno><idno type="pmid">27694423</idno><idno type="doi">10.1177/0300985816669407</idno><idno type="wicri:Area/PubMed/Corpus">000000</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000000</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Posttransplant Lymphoproliferative Disorders in Neuronal Xenotransplanted Macaques.</title><author><name sortKey="Cavicchioli, L" sort="Cavicchioli, L" uniqKey="Cavicchioli L" first="L" last="Cavicchioli">L. Cavicchioli</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Ferraresso, S" sort="Ferraresso, S" uniqKey="Ferraresso S" first="S" last="Ferraresso">S. Ferraresso</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Westmoreland, S" sort="Westmoreland, S" uniqKey="Westmoreland S" first="S" last="Westmoreland">S. Westmoreland</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kaliyaperumal, S" sort="Kaliyaperumal, S" uniqKey="Kaliyaperumal S" first="S" last="Kaliyaperumal">S. Kaliyaperumal</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Knight, H" sort="Knight, H" uniqKey="Knight H" first="H" last="Knight">H. Knight</name><affiliation><nlm:affiliation>2 New England Primate Research Center, Southborough, MA, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Crossan, C" sort="Crossan, C" uniqKey="Crossan C" first="C" last="Crossan">C. Crossan</name><affiliation><nlm:affiliation>3 Caledonian University, Glasgow, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Scobie, L" sort="Scobie, L" uniqKey="Scobie L" first="L" last="Scobie">L. Scobie</name><affiliation><nlm:affiliation>3 Caledonian University, Glasgow, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Danesi, A" sort="Danesi, A" uniqKey="Danesi A" first="A" last="Danesi">A. Danesi</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Vadori, M" sort="Vadori, M" uniqKey="Vadori M" first="M" last="Vadori">M. Vadori</name><affiliation><nlm:affiliation>4 CORIT, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Trez, D" sort="Trez, D" uniqKey="Trez D" first="D" last="Trez">D. Trez</name><affiliation><nlm:affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Badin, R Aron" sort="Badin, R Aron" uniqKey="Badin R" first="R Aron" last="Badin">R Aron Badin</name><affiliation><nlm:affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</nlm:affiliation></affiliation></author><author><name sortKey="Hantraye, P" sort="Hantraye, P" uniqKey="Hantraye P" first="P" last="Hantraye">P. Hantraye</name><affiliation><nlm:affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</nlm:affiliation></affiliation></author><author><name sortKey="Cozzi, E" sort="Cozzi, E" uniqKey="Cozzi E" first="E" last="Cozzi">E. Cozzi</name><affiliation><nlm:affiliation>4 CORIT, Padova, Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Veterinary pathology</title><idno type="eISSN">1544-2217</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of lymphoid proliferations that occur in the setting of depressed T-cell function due to immunosuppressive therapy used following solid organ transplantation, hematopoietic stem cell transplantation, and also xenotransplantation. In the present study, 28 immunosuppressed parkinsonian Macaca fascicularis were intracerebrally injected with wild-type or CTLA4-Ig transgenic porcine xenografts to identify a suitable strategy to enable long-term cell survival, maturation, and differentiation. Nine of 28 (32%) immunosuppressed primates developed masses compatible with PTLD, located mainly in the gastrointestinal tract and/or nasal cavity. The masses were classified as monomorphic PTLD according to the World Health Organization classification. Immunohistochemistry and polymerase chain reaction (PCR) analyses revealed that the PTLDs were associated with macaca lymphocryptovirus as confirmed by double-labeling immunohistochemistry for CD20 and Epstein-Barr nuclear antigen 2 (EBNA-2), where the viral protein was located within the CD20+ neoplastic B cells. In sera from 3 distinct phases of the experimental life of the primates, testing by quantitative PCR revealed a progression of the viral load that paralleled the PTLD progression and no evidence of zoonotic transmission of porcine lymphotropic herpesvirus through xenoneuronal grafts. These data suggest that monitoring the variation of macaca lymphocryptovirus DNA in primates could be used as a possible early diagnostic tool for PTLD progression, allowing preemptive treatment such as immunosuppression therapy reduction.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">27694423</PMID><DateCreated><Year>2016</Year><Month>10</Month><Day>03</Day></DateCreated><DateRevised><Year>2017</Year><Month>02</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1544-2217</ISSN><JournalIssue CitedMedium="Internet"><Volume>54</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Veterinary pathology</Title><ISOAbbreviation>Vet. Pathol.</ISOAbbreviation></Journal><ArticleTitle>Posttransplant Lymphoproliferative Disorders in Neuronal Xenotransplanted Macaques.</ArticleTitle><Pagination><MedlinePgn>336-344</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1177/0300985816669407</ELocationID><Abstract><AbstractText NlmCategory="UNASSIGNED">Posttransplant lymphoproliferative disorders (PTLDs) are a heterogeneous group of lymphoid proliferations that occur in the setting of depressed T-cell function due to immunosuppressive therapy used following solid organ transplantation, hematopoietic stem cell transplantation, and also xenotransplantation. In the present study, 28 immunosuppressed parkinsonian Macaca fascicularis were intracerebrally injected with wild-type or CTLA4-Ig transgenic porcine xenografts to identify a suitable strategy to enable long-term cell survival, maturation, and differentiation. Nine of 28 (32%) immunosuppressed primates developed masses compatible with PTLD, located mainly in the gastrointestinal tract and/or nasal cavity. The masses were classified as monomorphic PTLD according to the World Health Organization classification. Immunohistochemistry and polymerase chain reaction (PCR) analyses revealed that the PTLDs were associated with macaca lymphocryptovirus as confirmed by double-labeling immunohistochemistry for CD20 and Epstein-Barr nuclear antigen 2 (EBNA-2), where the viral protein was located within the CD20+ neoplastic B cells. In sera from 3 distinct phases of the experimental life of the primates, testing by quantitative PCR revealed a progression of the viral load that paralleled the PTLD progression and no evidence of zoonotic transmission of porcine lymphotropic herpesvirus through xenoneuronal grafts. These data suggest that monitoring the variation of macaca lymphocryptovirus DNA in primates could be used as a possible early diagnostic tool for PTLD progression, allowing preemptive treatment such as immunosuppression therapy reduction.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cavicchioli</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferraresso</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Westmoreland</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>2 New England Primate Research Center, Southborough, MA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaliyaperumal</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>2 New England Primate Research Center, Southborough, MA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Knight</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>2 New England Primate Research Center, Southborough, MA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Crossan</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>3 Caledonian University, Glasgow, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scobie</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>3 Caledonian University, Glasgow, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Danesi</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vadori</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>4 CORIT, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Trez</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>1 Department of Comparative Biomedicine and Food Science, Padova University, Padova, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Badin</LastName><ForeName>R Aron</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hantraye</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>5 MIRCen, CEA UMR 9199, Fontenay-aux-Roses, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cozzi</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>4 CORIT, Padova, Italy.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>6 Padova General Hospital, Padova, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>10</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Vet Pathol</MedlineTA><NlmUniqueID>0312020</NlmUniqueID><ISSNLinking>0300-9858</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lymphocryptovirus</Keyword><Keyword MajorTopicYN="N">Parkinson disease</Keyword><Keyword MajorTopicYN="N">cynomolgus macaques</Keyword><Keyword MajorTopicYN="N">immunosuppression</Keyword><Keyword MajorTopicYN="N">lymphoproliferative disorders</Keyword><Keyword MajorTopicYN="N">xenotransplantation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27694423</ArticleId><ArticleId IdType="pii">0300985816669407</ArticleId><ArticleId IdType="doi">10.1177/0300985816669407</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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