Catecholaminergic neurons result from intracerebral implantation of embryonal carcinoma cells.
Identifieur interne : 000309 ( Pmc/Curation ); précédent : 000308; suivant : 000310Catecholaminergic neurons result from intracerebral implantation of embryonal carcinoma cells.
Auteurs : B E Wojcik ; F. Nothias ; M. Lazar ; H. Jouin ; J F Nicolas ; M. PeschanskiSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1993.
Abstract
A replication-defective retrovirus was used to introduce the marker gene nlsLacZ into the murine embryonal carcinoma (EC) cell line PCC7-S-aza-R-1009. Undifferentiated EC cells were implanted into the central nervous system of adult rats. One month later, the grafted cells continued to express the nlsLacZ gene. Immunohistochemical analysis demonstrated the presence of EC-derived neurons. These neurons were capable of expressing tyrosine hydroxylase and extended neurites into the host parenchyma. EC-derived glial cells could not be detected. There was no evidence of tumorigenicity. These results demonstrate the utility of EC cells for introduction of exogenous gene products into the central nervous system in experimental models of gene therapy.
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PubMed: 8094557
PubMed Central: 45861
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn></journal-meta><article-meta><article-id pub-id-type="pmid">8094557</article-id><article-id pub-id-type="pmc">45861</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Catecholaminergic neurons result from intracerebral implantation of embryonal carcinoma cells.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wojcik</surname><given-names>B E</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nothias</surname><given-names>F</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lazar</surname><given-names>M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Jouin</surname><given-names>H</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nicolas</surname><given-names>J F</given-names></name></contrib><contrib contrib-type="author"><name><surname>Peschanski</surname><given-names>M</given-names></name></contrib></contrib-group><aff>Institut Pasteur, Centre National de Recherche Scientifique, Paris, France.</aff><pub-date pub-type="ppub"><day>15</day><month>2</month><year>1993</year></pub-date><volume>90</volume><issue>4</issue><fpage>1305</fpage><lpage>1309</lpage><abstract><p>A replication-defective retrovirus was used to introduce the marker gene nlsLacZ into the murine embryonal carcinoma (EC) cell line PCC7-S-aza-R-1009. Undifferentiated EC cells were implanted into the central nervous system of adult rats. One month later, the grafted cells continued to express the nlsLacZ gene. Immunohistochemical analysis demonstrated the presence of EC-derived neurons. These neurons were capable of expressing tyrosine hydroxylase and extended neurites into the host parenchyma. EC-derived glial cells could not be detected. There was no evidence of tumorigenicity. These results demonstrate the utility of EC cells for introduction of exogenous gene products into the central nervous system in experimental models of gene therapy.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="pnas01102-0178-a" xlink:role="1307"></graphic></fig><fig id="F2"><graphic xlink:href="pnas01102-0178-b" xlink:role="1307"></graphic></fig><fig id="F3"><graphic xlink:href="pnas01102-0179-a" xlink:role="1308"></graphic></fig><fig id="F4"><graphic xlink:href="pnas01102-0179-b" xlink:role="1308"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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