Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications
Identifieur interne : 000544 ( Pmc/Corpus ); précédent : 000543; suivant : 000545Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications
Auteurs : Enrico Cristante ; Simon Mcarthur ; Claudio Mauro ; Elisa Maggioli ; Ignacio A. Romero ; Marzena Wylezinska-Arridge ; Pierre O. Couraud ; Jordi Lopez-Tremoleda ; Helen C. Christian ; Babette B. Weksler ; Andrea Malaspina ; Egle SolitoSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2012.
Abstract
The blood–brain barrier (BBB), a critical guardian of communication between the periphery and the brain, is frequently compromised in neurological diseases such as multiple sclerosis (MS), resulting in the inappropriate passage of molecules and leukocytes into the brain. Here we show that the glucocorticoid anti-inflammatory messenger annexin A1 (ANXA1) is expressed in brain microvascular endothelial cells, where it regulates BBB integrity. In particular, ANXA1−/− mice exhibit significantly increased BBB permeability as a result of disrupted interendothelial cell tight junctions, essentially related to changes in the actin cytoskeleton, which stabilizes tight and adherens junctions. This situation is reminiscent of early MS pathology, a relationship confirmed by our detection of a selective loss of ANXA1 in the plasma and cerebrovascular endothelium of patients with MS. Importantly, this loss is swiftly restored by i.v. administration of human recombinant ANXA1. Analysis in vitro confirms that treatment of cerebrovascular endothelial cells with recombinant ANXA1 restores cell polarity, cytoskeleton integrity, and paracellular permeability through inhibition of the small G protein RhoA. We thus propose ANXA1 as a critical physiological regulator of BBB integrity and suggest it may have utility in the treatment of MS, correcting BBB function and hence ameliorating disease.
Url:
DOI: 10.1073/pnas.1209362110
PubMed: 23277546
PubMed Central: 3549094
Links to Exploration step
PMC:3549094Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications</title>
<author><name sortKey="Cristante, Enrico" sort="Cristante, Enrico" uniqKey="Cristante E" first="Enrico" last="Cristante">Enrico Cristante</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mcarthur, Simon" sort="Mcarthur, Simon" uniqKey="Mcarthur S" first="Simon" last="Mcarthur">Simon Mcarthur</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mauro, Claudio" sort="Mauro, Claudio" uniqKey="Mauro C" first="Claudio" last="Mauro">Claudio Mauro</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Maggioli, Elisa" sort="Maggioli, Elisa" uniqKey="Maggioli E" first="Elisa" last="Maggioli">Elisa Maggioli</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Romero, Ignacio A" sort="Romero, Ignacio A" uniqKey="Romero I" first="Ignacio A." last="Romero">Ignacio A. Romero</name>
<affiliation><nlm:aff id="aff2">Department of Life Sciences,<institution>The Open Universit</institution>
y, Milton Keynes MK7 6AA,<country>United Kingdom;</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wylezinska Arridge, Marzena" sort="Wylezinska Arridge, Marzena" uniqKey="Wylezinska Arridge M" first="Marzena" last="Wylezinska-Arridge">Marzena Wylezinska-Arridge</name>
<affiliation><nlm:aff id="aff3">Institute of Clinical Science,<institution>Imperial College London</institution>
, London W12 0NN,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Couraud, Pierre O" sort="Couraud, Pierre O" uniqKey="Couraud P" first="Pierre O." last="Couraud">Pierre O. Couraud</name>
<affiliation><nlm:aff id="aff4">INSERM U1016, Institut Cochin,<institution>Université Paris Descartes</institution>
, 75014 Paris,<country>France</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lopez Tremoleda, Jordi" sort="Lopez Tremoleda, Jordi" uniqKey="Lopez Tremoleda J" first="Jordi" last="Lopez-Tremoleda">Jordi Lopez-Tremoleda</name>
<affiliation><nlm:aff id="aff3">Institute of Clinical Science,<institution>Imperial College London</institution>
, London W12 0NN,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Christian, Helen C" sort="Christian, Helen C" uniqKey="Christian H" first="Helen C." last="Christian">Helen C. Christian</name>
<affiliation><nlm:aff id="aff5">Department of Physiology, Anatomy and Genetics,<institution>University of Oxford</institution>
, Oxford OX1 3QX,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Weksler, Babette B" sort="Weksler, Babette B" uniqKey="Weksler B" first="Babette B." last="Weksler">Babette B. Weksler</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff6">Division of Haematology and Medical Oncology,<institution>Weill Medical College of Cornell University</institution>
, New York,<addr-line>NY</addr-line>
10065</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Malaspina, Andrea" sort="Malaspina, Andrea" uniqKey="Malaspina A" first="Andrea" last="Malaspina">Andrea Malaspina</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff7">North-East London and Essex MND Care and Research Centre</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff8">Centre for Neuroscience and Trauma, Blizard Institute,<institution>Queen Mary University of London</institution>
, London E1 2AT,<country>United Kingdom</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Solito, Egle" sort="Solito, Egle" uniqKey="Solito E" first="Egle" last="Solito">Egle Solito</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">23277546</idno>
<idno type="pmc">3549094</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3549094</idno>
<idno type="RBID">PMC:3549094</idno>
<idno type="doi">10.1073/pnas.1209362110</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">000544</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000544</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications</title>
<author><name sortKey="Cristante, Enrico" sort="Cristante, Enrico" uniqKey="Cristante E" first="Enrico" last="Cristante">Enrico Cristante</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mcarthur, Simon" sort="Mcarthur, Simon" uniqKey="Mcarthur S" first="Simon" last="Mcarthur">Simon Mcarthur</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mauro, Claudio" sort="Mauro, Claudio" uniqKey="Mauro C" first="Claudio" last="Mauro">Claudio Mauro</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Maggioli, Elisa" sort="Maggioli, Elisa" uniqKey="Maggioli E" first="Elisa" last="Maggioli">Elisa Maggioli</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Romero, Ignacio A" sort="Romero, Ignacio A" uniqKey="Romero I" first="Ignacio A." last="Romero">Ignacio A. Romero</name>
<affiliation><nlm:aff id="aff2">Department of Life Sciences,<institution>The Open Universit</institution>
y, Milton Keynes MK7 6AA,<country>United Kingdom;</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wylezinska Arridge, Marzena" sort="Wylezinska Arridge, Marzena" uniqKey="Wylezinska Arridge M" first="Marzena" last="Wylezinska-Arridge">Marzena Wylezinska-Arridge</name>
<affiliation><nlm:aff id="aff3">Institute of Clinical Science,<institution>Imperial College London</institution>
, London W12 0NN,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Couraud, Pierre O" sort="Couraud, Pierre O" uniqKey="Couraud P" first="Pierre O." last="Couraud">Pierre O. Couraud</name>
<affiliation><nlm:aff id="aff4">INSERM U1016, Institut Cochin,<institution>Université Paris Descartes</institution>
, 75014 Paris,<country>France</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lopez Tremoleda, Jordi" sort="Lopez Tremoleda, Jordi" uniqKey="Lopez Tremoleda J" first="Jordi" last="Lopez-Tremoleda">Jordi Lopez-Tremoleda</name>
<affiliation><nlm:aff id="aff3">Institute of Clinical Science,<institution>Imperial College London</institution>
, London W12 0NN,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Christian, Helen C" sort="Christian, Helen C" uniqKey="Christian H" first="Helen C." last="Christian">Helen C. Christian</name>
<affiliation><nlm:aff id="aff5">Department of Physiology, Anatomy and Genetics,<institution>University of Oxford</institution>
, Oxford OX1 3QX,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Weksler, Babette B" sort="Weksler, Babette B" uniqKey="Weksler B" first="Babette B." last="Weksler">Babette B. Weksler</name>
<affiliation><nlm:aff wicri:cut="; and" id="aff6">Division of Haematology and Medical Oncology,<institution>Weill Medical College of Cornell University</institution>
, New York,<addr-line>NY</addr-line>
10065</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Malaspina, Andrea" sort="Malaspina, Andrea" uniqKey="Malaspina A" first="Andrea" last="Malaspina">Andrea Malaspina</name>
<affiliation><nlm:aff wicri:cut=" and" id="aff7">North-East London and Essex MND Care and Research Centre</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="aff8">Centre for Neuroscience and Trauma, Blizard Institute,<institution>Queen Mary University of London</institution>
, London E1 2AT,<country>United Kingdom</country>
</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Solito, Egle" sort="Solito, Egle" uniqKey="Solito E" first="Egle" last="Solito">Egle Solito</name>
<affiliation><nlm:aff id="aff1">William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The blood–brain barrier (BBB), a critical guardian of communication between the periphery and the brain, is frequently compromised in neurological diseases such as multiple sclerosis (MS), resulting in the inappropriate passage of molecules and leukocytes into the brain. Here we show that the glucocorticoid anti-inflammatory messenger annexin A1 (ANXA1) is expressed in brain microvascular endothelial cells, where it regulates BBB integrity. In particular, ANXA1<sup>−/−</sup>
mice exhibit significantly increased BBB permeability as a result of disrupted interendothelial cell tight junctions, essentially related to changes in the actin cytoskeleton, which stabilizes tight and adherens junctions. This situation is reminiscent of early MS pathology, a relationship confirmed by our detection of a selective loss of ANXA1 in the plasma and cerebrovascular endothelium of patients with MS. Importantly, this loss is swiftly restored by i.v. administration of human recombinant ANXA1. Analysis in vitro confirms that treatment of cerebrovascular endothelial cells with recombinant ANXA1 restores cell polarity, cytoskeleton integrity, and paracellular permeability through inhibition of the small G protein RhoA. We thus propose ANXA1 as a critical physiological regulator of BBB integrity and suggest it may have utility in the treatment of MS, correcting BBB function and hence ameliorating disease.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id>
<journal-id journal-id-type="iso-abbrev">Proc. Natl. Acad. Sci. U.S.A</journal-id>
<journal-id journal-id-type="hwp">pnas</journal-id>
<journal-id journal-id-type="pmc">pnas</journal-id>
<journal-id journal-id-type="publisher-id">PNAS</journal-id>
<journal-title-group><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title>
</journal-title-group>
<issn pub-type="ppub">0027-8424</issn>
<issn pub-type="epub">1091-6490</issn>
<publisher><publisher-name>National Academy of Sciences</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23277546</article-id>
<article-id pub-id-type="pmc">3549094</article-id>
<article-id pub-id-type="publisher-id">201209362</article-id>
<article-id pub-id-type="doi">10.1073/pnas.1209362110</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Feature Article</subject>
</subj-group>
<subj-group subj-group-type="heading"><subject>Biological Sciences</subject>
<subj-group><subject>Physiology</subject>
</subj-group>
</subj-group>
<series-title>From the Cover</series-title>
<series-title>Feature Article</series-title>
</article-categories>
<title-group><article-title>Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications</article-title>
<alt-title alt-title-type="short">Annexin A1, a regulator of BBB integrity</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Cristante</surname>
<given-names>Enrico</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>McArthur</surname>
<given-names>Simon</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="author-notes" rid="fn1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mauro</surname>
<given-names>Claudio</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Maggioli</surname>
<given-names>Elisa</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Romero</surname>
<given-names>Ignacio A.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>b</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wylezinska-Arridge</surname>
<given-names>Marzena</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Couraud</surname>
<given-names>Pierre O.</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>d</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lopez-Tremoleda</surname>
<given-names>Jordi</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>c</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Christian</surname>
<given-names>Helen C.</given-names>
</name>
<xref ref-type="aff" rid="aff5"><sup>e</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Weksler</surname>
<given-names>Babette B.</given-names>
</name>
<xref ref-type="aff" rid="aff6"><sup>f</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Malaspina</surname>
<given-names>Andrea</given-names>
</name>
<xref ref-type="aff" rid="aff7"><sup>g</sup>
</xref>
<xref ref-type="aff" rid="aff8"><sup>h</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Solito</surname>
<given-names>Egle</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>a</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>2</sup>
</xref>
</contrib>
<aff id="aff1"><sup>a</sup>
William Harvey Research Institute, Barts and the London School of Medicine and Dentistry,<institution>Queen Mary University of London</institution>
, London EC1M 6BQ,<country>United Kingdom</country>
;</aff>
<aff id="aff2"><sup>b</sup>
Department of Life Sciences,<institution>The Open Universit</institution>
y, Milton Keynes MK7 6AA,<country>United Kingdom;</country>
</aff>
<aff id="aff3"><sup>c</sup>
Institute of Clinical Science,<institution>Imperial College London</institution>
, London W12 0NN,<country>United Kingdom</country>
;</aff>
<aff id="aff4"><sup>d</sup>
INSERM U1016, Institut Cochin,<institution>Université Paris Descartes</institution>
, 75014 Paris,<country>France</country>
;</aff>
<aff id="aff5"><sup>e</sup>
Department of Physiology, Anatomy and Genetics,<institution>University of Oxford</institution>
, Oxford OX1 3QX,<country>United Kingdom</country>
;</aff>
<aff id="aff6"><sup>f</sup>
Division of Haematology and Medical Oncology,<institution>Weill Medical College of Cornell University</institution>
, New York,<addr-line>NY</addr-line>
10065; and</aff>
<aff id="aff7"><sup>g</sup>
North-East London and Essex MND Care and Research Centre and</aff>
<aff id="aff8"><sup>h</sup>
Centre for Neuroscience and Trauma, Blizard Institute,<institution>Queen Mary University of London</institution>
, London E1 2AT,<country>United Kingdom</country>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><sup>2</sup>
To whom correspondence should be addressed. E-mail: <email>e.solito@qmul.ac.uk</email>
.</corresp>
<fn fn-type="edited-by"><p>This Feature Article is part of a series identified by the Editorial Board as reporting findings of exceptional significance.</p>
</fn>
<fn fn-type="edited-by"><p>Edited by Bruce S. McEwen, The Rockefeller University, New York, NY, and approved November 26, 2012 (received for review June 4, 2012)</p>
</fn>
<fn fn-type="con"><p>Author contributions: E.S. designed research; E.C., S.M., C.M., E.M., M.W.-A., J.L.-T., H.C.C., and E.S. performed research; I.A.R., P.O.C., B.B.W., A.M., and E.S. contributed new reagents/analytic tools; E.C., S.M., and E.S. analyzed data; and S.M. and E.S. wrote the paper.</p>
</fn>
<fn fn-type="equal" id="fn1"><p><sup>1</sup>
E.C. and S.M. contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><day>15</day>
<month>1</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>31</day>
<month>12</month>
<year>2012</year>
</pub-date>
<volume>110</volume>
<issue>3</issue>
<fpage>832</fpage>
<lpage>841</lpage>
<self-uri xlink:title="pdf" xlink:type="simple" xlink:href="pnas.201209362.pdf"></self-uri>
<abstract><p>The blood–brain barrier (BBB), a critical guardian of communication between the periphery and the brain, is frequently compromised in neurological diseases such as multiple sclerosis (MS), resulting in the inappropriate passage of molecules and leukocytes into the brain. Here we show that the glucocorticoid anti-inflammatory messenger annexin A1 (ANXA1) is expressed in brain microvascular endothelial cells, where it regulates BBB integrity. In particular, ANXA1<sup>−/−</sup>
mice exhibit significantly increased BBB permeability as a result of disrupted interendothelial cell tight junctions, essentially related to changes in the actin cytoskeleton, which stabilizes tight and adherens junctions. This situation is reminiscent of early MS pathology, a relationship confirmed by our detection of a selective loss of ANXA1 in the plasma and cerebrovascular endothelium of patients with MS. Importantly, this loss is swiftly restored by i.v. administration of human recombinant ANXA1. Analysis in vitro confirms that treatment of cerebrovascular endothelial cells with recombinant ANXA1 restores cell polarity, cytoskeleton integrity, and paracellular permeability through inhibition of the small G protein RhoA. We thus propose ANXA1 as a critical physiological regulator of BBB integrity and suggest it may have utility in the treatment of MS, correcting BBB function and hence ameliorating disease.</p>
</abstract>
<counts><page-count count="10"></page-count>
</counts>
</article-meta>
</front>
</pmc>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000544 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000544 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Pmc |étape= Corpus |type= RBID |clé= PMC:3549094 |texte= Identification of an essential endogenous regulator of blood–brain barrier integrity, and its pathological and therapeutic implications }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:23277546" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a ParkinsonFranceV1
![]() | This area was generated with Dilib version V0.6.29. | ![]() |