Survival, Differentiation, and Neuroprotective Mechanisms of Human Stem Cells Complexed With Neurotrophin-3-Releasing Pharmacologically Active Microcarriers in an Ex Vivo Model of Parkinson’s Disease
Identifieur interne : 000053 ( Pmc/Corpus ); précédent : 000052; suivant : 000054Survival, Differentiation, and Neuroprotective Mechanisms of Human Stem Cells Complexed With Neurotrophin-3-Releasing Pharmacologically Active Microcarriers in an Ex Vivo Model of Parkinson’s Disease
Auteurs : Nicolas Daviaud ; Elisa Garbayo ; Laurence Sindji ; Alberto Martínez-Serrano ; Paul C. Schiller ; Claudia N. Montero-MeneiSource :
- Stem Cells Translational Medicine [ 2157-6564 ] ; 2015.
Abstract
The survival, differentiation, and neuroprotective mechanisms of marrow-isolated adult multilineage inducible (MIAMI) cells and neural stem cells (NSCs), both adhering to neurotrophin-3 releasing pharmacologically active microcarriers (PAMs) in an ex vivo organotypic model of nigrostriatal degeneration made from brain sagittal slices were elucidated. The results indicate a prospective interest of human NSC/PAM and MIAMI cell/PAM complexes in tissue engineering for Parkinson’s disease.
Url:
DOI: 10.5966/sctm.2014-0139
PubMed: 25925835
PubMed Central: 4449089
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Stem Cells Transl Med</journal-id><journal-id journal-id-type="iso-abbrev">Stem Cells Transl Med</journal-id><journal-id journal-id-type="pmc">Stem Cells Translational Medicine</journal-id><journal-id journal-id-type="hwp">sctm</journal-id><journal-id journal-id-type="publisher-id">Stem Cells Translational Medicine</journal-id><journal-title-group><journal-title>Stem Cells Translational Medicine</journal-title></journal-title-group><issn pub-type="ppub">2157-6564</issn><issn pub-type="epub">2157-6580</issn><publisher><publisher-name>AlphaMed Press</publisher-name><publisher-loc>Durham, NC, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25925835</article-id><article-id pub-id-type="pmc">4449089</article-id><article-id pub-id-type="publisher-id">20140139</article-id><article-id pub-id-type="doi">10.5966/sctm.2014-0139</article-id><article-categories><subj-group subj-group-type="hwp-journal-coll"><subject>11</subject><subject>38</subject></subj-group><subj-group subj-group-type="heading"><subject>Tissue Engineering and Regenerative Medicine</subject></subj-group></article-categories><title-group><article-title>Survival, Differentiation, and Neuroprotective Mechanisms of Human Stem Cells Complexed With Neurotrophin-3-Releasing Pharmacologically Active Microcarriers in an Ex Vivo Model of Parkinson’s Disease</article-title><alt-title alt-title-type="short">PAMs—Stem Cell Complexes in Parkinson’s Disease</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Daviaud</surname><given-names>Nicolas</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Garbayo</surname><given-names>Elisa</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="aff" rid="aff3"><sup>c</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sindji</surname><given-names>Laurence</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Martínez-Serrano</surname><given-names>Alberto</given-names></name><xref ref-type="aff" rid="aff4"><sup>d</sup></xref></contrib><contrib contrib-type="author"><name><surname>Schiller</surname><given-names>Paul C.</given-names></name><xref ref-type="aff" rid="aff5"><sup>e</sup></xref><xref ref-type="aff" rid="aff6"><sup>f</sup></xref><xref ref-type="aff" rid="aff7"><sup>g</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Montero-Menei</surname><given-names>Claudia N.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><aff><target id="aff1" target-type="aff"><sup>a</sup></target>INSERM U1066, Micro et nanomédecines biomimétiques, Angers, France;<target id="aff2" target-type="aff"><sup>b</sup></target>L’université Nantes, Angers, Le Mans, Angers University, Angers, France;<target id="aff3" target-type="aff"><sup>c</sup></target>Pharmacy and Pharmaceutical Technology Department, University of Navarra, Pamplona, Spain;<target id="aff4" target-type="aff"><sup>d</sup></target>Department of Molecular Biology and Center of Molecular Biology “Severo Ochoa,” Autonomous University of Madrid-Consejo Superior de Investigaciones Científicas, Campus Cantoblanco, Madrid, Spain;<target id="aff5" target-type="aff"><sup>e</sup></target>Miami Veterans Healthcare System,<target id="aff6" target-type="aff"><sup>f</sup></target>Department of Orthopedics, and<target id="aff7" target-type="aff"><sup>g</sup></target>Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA</aff></contrib-group><author-notes><corresp id="cor1">Correspondence: Claudia N. Montero-Menei, Ph.D., INSERM U1066, IBS-CHU Angers, 4 rue Larrey, 49933 Angers Cx 9, France. Telephone: <phone>33-0-2-4468-8536</phone>; E-Mail: <email>claudia.montero-menei@univ-angers.fr</email></corresp></author-notes><pub-date pub-type="ppub"><month>6</month><year>2015</year></pub-date><pub-date pub-type="epub"><day>29</day><month>4</month><year>2015</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>6</month><year>2016</year></pub-date><pmc-comment> PMC Release delay is 12 months and
0 days and was based on the
<volume>4</volume><issue>6</issue><fpage>670</fpage><lpage>684</lpage><history><date date-type="received"><day>11</day><month>7</month><year>2014</year></date><date date-type="accepted"><day>05</day><month>3</month><year>2015</year></date></history><permissions><copyright-statement>©AlphaMed Press</copyright-statement><copyright-year>2015</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="sctm_20140139.pdf"></self-uri><abstract abstract-type="precis"><p>The survival, differentiation, and neuroprotective mechanisms of marrow-isolated adult multilineage inducible (MIAMI) cells and neural stem cells (NSCs), both adhering to neurotrophin-3 releasing pharmacologically active microcarriers (PAMs) in an ex vivo organotypic model of nigrostriatal degeneration made from brain sagittal slices were elucidated. The results indicate a prospective interest of human NSC/PAM and MIAMI cell/PAM complexes in tissue engineering for Parkinson’s disease.</p></abstract><abstract><sec><title></title><p>Stem cell-based regenerative therapies hold great potential for the treatment of degenerative disorders such as Parkinson’s disease (PD). We recently reported the repair and functional recovery after treatment with human marrow-isolated adult multilineage inducible (MIAMI) cells adhered to neurotrophin-3 (NT3) releasing pharmacologically active microcarriers (PAMs) in hemiparkinsonian rats. In order to comprehend this effect, the goal of the present work was to elucidate the survival, differentiation, and neuroprotective mechanisms of MIAMI cells and human neural stem cells (NSCs), both adhering to NT3-releasing PAMs in an ex vivo organotypic model of nigrostriatal degeneration made from brain sagittal slices. It was shown that PAMs led to a marked increase in MIAMI cell survival and neuronal differentiation when releasing NT3. A significant neuroprotective effect of MIAMI cells adhering to PAMs was also demonstrated. NSCs barely had a neuroprotective effect and differentiated mostly into dopaminergic neuronal cells when adhering to PAM-NT3. Moreover, those cells were able to release dopamine in a sufficient amount to induce a return to baseline levels. Reverse transcription-quantitative polymerase chain reaction and enzyme-linked immunosorbent assay analyses identified vascular endothelial growth factor (VEGF) and stanniocalcin-1 as potential mediators of the neuroprotective effect of MIAMI cells and NSCs, respectively. It was also shown that VEGF locally stimulated tissue vascularization, which might improve graft survival, without excluding a direct neuroprotective effect of VEGF on dopaminergic neurons. These results indicate a prospective interest of human NSC/PAM and MIAMI cell/PAM complexes in tissue engineering for PD.</p></sec><sec><title>Significance</title><p>Stem cell-based regenerative therapies hold great potential for the treatment of degenerative disorders such as Parkinson’s disease (PD). The present work elucidates and compares the survival, differentiation, and neuroprotective mechanisms of marrow-isolated adult multilineage inducible cells and human neural stem cells both adhered to neurotrophin-3-releasing pharmacologically active microcarriers in an ex vivo organotypic model of PD made from brain sagittal slices.</p></sec></abstract><kwd-group><kwd>Neural stem cells</kwd><kwd>Mesenchymal stem cells</kwd><kwd>Organotypic slice culture</kwd><kwd>Neurotrophin-3</kwd><kwd>Laminin</kwd><kwd>Tissue engineering</kwd><kwd>Vascular endothelial growth factor</kwd><kwd>Stanniocalcin-1</kwd></kwd-group><counts><page-count count="15"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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