Enantio-specific induction of apoptosis by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopaminergic SH-SY5Y cells : Suppression of apoptosis by N-(2-heptyl)-N-methylpropargylamine
Identifieur interne : 001069 ( PascalFrancis/Checkpoint ); précédent : 001068; suivant : 001070Enantio-specific induction of apoptosis by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopaminergic SH-SY5Y cells : Suppression of apoptosis by N-(2-heptyl)-N-methylpropargylamine
Auteurs : W. Maruyama [Japon] ; A. A. Boulton [Canada] ; B. A. Davis [Canada] ; P. Dostert [France] ; M. Naoi [Japon]Source :
- Journal of neural transmission [ 0300-9564 ] ; 2001.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
Endogenous N-methyl(R)salsolinol, which caused parkinsonism in rats by injection in the striatum, was found to induce apoptosis in dopaminergic neuroblastoma SH-SY5Y cells. After 12-h incubation with 500 μM N-methyl(R)salsolinol, almost all the cells died with apoptosis and necrotic cell death was negligible. N-Methyl(R)salsolinol was much more potent to induce apoptosis than the (S)-enantiomer. The mechanism of apoptosis was studied in relation to changes in mitochondrial membrane potential, ΔΨm, using a fluorescent indicator, JC-1. Red fluorescence of J-aggregates representing hyperpolarized ΔΨm was found to decrease significantly within 60min after incubation with N-methyl(R)salsolinol, but not by the (S)-enantiomer at the same concentration. It suggests that mitochondria may recognize the stereo-chemical structure of N-methyl(R) salsolinol. Aliphatic propargylamines, (R)-N-(2-heptyl)-N-methylpropargyl-amine and (R)-N-(2-heptyl)propargylamine, were found to prevent ΔΨm loss and subsequent apoptosis induced by N-methyl(R)salsolinol. These results suggest that mitochondria play a key role in the induction of apoptosis by the neurotoxin and the prevention by aliphatic propargylamines.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Enantio-specific induction of apoptosis by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopaminergic SH-SY5Y cells : Suppression of apoptosis by N-(2-heptyl)-N-methylpropargylamine</title><author><name sortKey="Maruyama, W" sort="Maruyama, W" uniqKey="Maruyama W" first="W." last="Maruyama">W. Maruyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Biochemistry and Metabolism, Department of Basic Gerontology, National Institute for Longevity Sciences</s1><s2>Obu, Aichi</s2><s3>JPN</s3><sZ>1 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Obu, Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Boulton, A A" sort="Boulton, A A" uniqKey="Boulton A" first="A. A." last="Boulton">A. A. Boulton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan</s1><s2>Saskatoon, Saskatchewan</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Davis, B A" sort="Davis, B A" uniqKey="Davis B" first="B. A." last="Davis">B. A. Davis</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan</s1><s2>Saskatoon, Saskatchewan</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Dostert, P" sort="Dostert, P" uniqKey="Dostert P" first="P." last="Dostert">P. Dostert</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Biotrial</s1><s2>Rennes</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Naoi, M" sort="Naoi, M" uniqKey="Naoi M" first="M." last="Naoi">M. Naoi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Brain Sciences, Institute of Applied Biochemistry, Yagi Memorial Park</s1><s2>Mitake, Gifu</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Mitake, Gifu</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">01-0115994</idno><date when="2001">2001</date><idno type="stanalyst">PASCAL 01-0115994 INIST</idno><idno type="RBID">Pascal:01-0115994</idno><idno type="wicri:Area/PascalFrancis/Corpus">001262</idno><idno type="wicri:Area/PascalFrancis/Curation">000176</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">001069</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">001069</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Enantio-specific induction of apoptosis by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopaminergic SH-SY5Y cells : Suppression of apoptosis by N-(2-heptyl)-N-methylpropargylamine</title><author><name sortKey="Maruyama, W" sort="Maruyama, W" uniqKey="Maruyama W" first="W." last="Maruyama">W. Maruyama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratory of Biochemistry and Metabolism, Department of Basic Gerontology, National Institute for Longevity Sciences</s1><s2>Obu, Aichi</s2><s3>JPN</s3><sZ>1 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Obu, Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Boulton, A A" sort="Boulton, A A" uniqKey="Boulton A" first="A. A." last="Boulton">A. A. Boulton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan</s1><s2>Saskatoon, Saskatchewan</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Davis, B A" sort="Davis, B A" uniqKey="Davis B" first="B. A." last="Davis">B. A. Davis</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Neuropsychiatry Research Unit, Department of Psychiatry, University of Saskatchewan</s1><s2>Saskatoon, Saskatchewan</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Dostert, P" sort="Dostert, P" uniqKey="Dostert P" first="P." last="Dostert">P. Dostert</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Biotrial</s1><s2>Rennes</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Région Bretagne</region><region type="old region">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Naoi, M" sort="Naoi, M" uniqKey="Naoi M" first="M." last="Naoi">M. Naoi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Brain Sciences, Institute of Applied Biochemistry, Yagi Memorial Park</s1><s2>Mitake, Gifu</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Mitake, Gifu</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of neural transmission</title><title level="j" type="abbreviated">J. neural transm.</title><idno type="ISSN">0300-9564</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of neural transmission</title><title level="j" type="abbreviated">J. neural transm.</title><idno type="ISSN">0300-9564</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis</term><term>Dopaminergic neuron</term><term>Enantiomer</term><term>Membrane potential</term><term>Mitochondria</term><term>Neurotoxin</term><term>Parkinson disease</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Neurotoxine</term><term>Enantiomère</term><term>Neurone dopaminergique</term><term>Apoptose</term><term>Potentiel membrane</term><term>Mitochondrie</term><term>Parkinson maladie</term><term>Salsolinol(N-méthyl)</term><term>Lignée SHSY5Y</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Endogenous N-methyl(R)salsolinol, which caused parkinsonism in rats by injection in the striatum, was found to induce apoptosis in dopaminergic neuroblastoma SH-SY5Y cells. After 12-h incubation with 500 μM N-methyl(R)salsolinol, almost all the cells died with apoptosis and necrotic cell death was negligible. N-Methyl(R)salsolinol was much more potent to induce apoptosis than the (S)-enantiomer. The mechanism of apoptosis was studied in relation to changes in mitochondrial membrane potential, ΔΨm, using a fluorescent indicator, JC-1. Red fluorescence of J-aggregates representing hyperpolarized ΔΨm was found to decrease significantly within 60min after incubation with N-methyl(R)salsolinol, but not by the (S)-enantiomer at the same concentration. It suggests that mitochondria may recognize the stereo-chemical structure of N-methyl(R) salsolinol. Aliphatic propargylamines, (R)-N-(2-heptyl)-N-methylpropargyl-amine and (R)-N-(2-heptyl)propargylamine, were found to prevent ΔΨm loss and subsequent apoptosis induced by N-methyl(R)salsolinol. These results suggest that mitochondria play a key role in the induction of apoptosis by the neurotoxin and the prevention by aliphatic propargylamines.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0300-9564</s0></fA01><fA02 i1="01"><s0>JNTMAH</s0></fA02><fA03 i2="1"><s0>J. neural transm.</s0></fA03><fA05><s2>108</s2></fA05><fA06><s2>1</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Enantio-specific induction of apoptosis by an endogenous neurotoxin, N-methyl(R)salsolinol, in dopaminergic SH-SY5Y cells : Suppression of apoptosis by N-(2-heptyl)-N-methylpropargylamine</s1></fA08><fA11 i1="01" i2="1"><s1>MARUYAMA (W.)</s1></fA11><fA11 i1="02" i2="1"><s1>BOULTON (A. A.)</s1></fA11><fA11 i1="03" i2="1"><s1>DAVIS (B. 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Red fluorescence of J-aggregates representing hyperpolarized ΔΨm was found to decrease significantly within 60min after incubation with N-methyl(R)salsolinol, but not by the (S)-enantiomer at the same concentration. It suggests that mitochondria may recognize the stereo-chemical structure of N-methyl(R) salsolinol. Aliphatic propargylamines, (R)-N-(2-heptyl)-N-methylpropargyl-amine and (R)-N-(2-heptyl)propargylamine, were found to prevent ΔΨm loss and subsequent apoptosis induced by N-methyl(R)salsolinol. 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Maruyama</name></noRegion><name sortKey="Naoi, M" sort="Naoi, M" uniqKey="Naoi M" first="M." last="Naoi">M. Naoi</name></country><country name="Canada"><noRegion><name sortKey="Boulton, A A" sort="Boulton, A A" uniqKey="Boulton A" first="A. A." last="Boulton">A. A. Boulton</name></noRegion><name sortKey="Davis, B A" sort="Davis, B A" uniqKey="Davis B" first="B. A." last="Davis">B. A. Davis</name></country><country name="France"><region name="Région Bretagne"><name sortKey="Dostert, P" sort="Dostert, P" uniqKey="Dostert P" first="P." last="Dostert">P. Dostert</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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