Qualitative and Quantitative Multiplexed Proteomic Analysis of Complex Yeast Protein Fractions That Modulate the Assembly of the Yeast Prion Sup35p
Identifieur interne : 000D82 ( Ncbi/Merge ); précédent : 000D81; suivant : 000D83Qualitative and Quantitative Multiplexed Proteomic Analysis of Complex Yeast Protein Fractions That Modulate the Assembly of the Yeast Prion Sup35p
Auteurs : Virginie Redeker [France] ; Chris Hughes [Royaume-Uni] ; Jimmy Savistchenko [France] ; Johannes P. C. Vissers [Royaume-Uni] ; Ronald Melki [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2011.
Abstract
The aggregation of the baker's yeast prion Sup35p is at the origin of the transmissible [
We designed a functional proteomic study that combines two techniques to identify modulators of Sup35p aggregation and describe the changes associated to [
Our functional proteomic study provides the first inventory list of over 300 proteins that directly or indirectly affect Sup35p aggregation and [
Url:
DOI: 10.1371/journal.pone.0023659
PubMed: 21931608
PubMed Central: 3172207
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Qualitative and Quantitative Multiplexed Proteomic Analysis of Complex Yeast Protein Fractions That Modulate the Assembly of the Yeast Prion Sup35p</title>
<author><name sortKey="Redeker, Virginie" sort="Redeker, Virginie" uniqKey="Redeker V" first="Virginie" last="Redeker">Virginie Redeker</name>
<affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France</addr-line>
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<author><name sortKey="Vissers, Johannes P C" sort="Vissers, Johannes P C" uniqKey="Vissers J" first="Johannes P. C." last="Vissers">Johannes P. C. Vissers</name>
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<author><name sortKey="Melki, Ronald" sort="Melki, Ronald" uniqKey="Melki R" first="Ronald" last="Melki">Ronald Melki</name>
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<country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif-sur-Yvette</wicri:regionArea>
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<series><title level="j">PLoS ONE</title>
<idno type="eISSN">1932-6203</idno>
<imprint><date when="2011">2011</date>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>The aggregation of the baker's yeast prion Sup35p is at the origin of the transmissible [<italic>PSI<sup>+</sup>
</italic>
] trait. We and others have shown that molecular chaperones modulate Sup35p aggregation. However, other protein classes might be involved in [<italic>PSI<sup>+</sup>
</italic>
] formation.</p>
</sec>
<sec><title>Results</title>
<p>We designed a functional proteomic study that combines two techniques to identify modulators of Sup35p aggregation and describe the changes associated to [<italic>PSI<sup>+</sup>
</italic>
] formation. The first allows measuring the effect of fractionated <italic>Saccharomyces cerevisiae</italic>
cytosolic extracts from [<italic>PSI<sup>+</sup>
</italic>
] and [<italic>psi<sup>−</sup>
</italic>
] yeast cells on Sup35p assembly. The second is a multiplex qualitative and quantitative comparison of protein composition of active and inactive fractions using a gel-free and label-free LC-MS approach. We identify changes in proteins involved in translation, folding, degradation, oxido-reduction and metabolic processes.</p>
</sec>
<sec><title>Conclusion</title>
<p>Our functional proteomic study provides the first inventory list of over 300 proteins that directly or indirectly affect Sup35p aggregation and [<italic>PSI<sup>+</sup>
</italic>
] formation. Our results highlight the complexity of the cellular changes accompanying [<italic>PSI<sup>+</sup>
</italic>
] formation and pave the way for <italic>in vitro</italic>
studies aimed to document the effect of individual and/or combinations of proteins identified here, susceptible of affecting Sup35p assembly.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id>
<journal-id journal-id-type="publisher-id">plos</journal-id>
<journal-id journal-id-type="pmc">plosone</journal-id>
<journal-title-group><journal-title>PLoS ONE</journal-title>
</journal-title-group>
<issn pub-type="epub">1932-6203</issn>
<publisher><publisher-name>Public Library of Science</publisher-name>
<publisher-loc>San Francisco, USA</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">21931608</article-id>
<article-id pub-id-type="pmc">3172207</article-id>
<article-id pub-id-type="publisher-id">PONE-D-11-06726</article-id>
<article-id pub-id-type="doi">10.1371/journal.pone.0023659</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
<subj-group subj-group-type="Discipline-v2"><subject>Biology</subject>
<subj-group><subject>Biochemistry</subject>
<subj-group><subject>Proteins</subject>
<subj-group><subject>Chaperone Proteins</subject>
<subject>Protein Interactions</subject>
<subject>Proteome</subject>
</subj-group>
</subj-group>
</subj-group>
<subj-group><subject>Biophysics</subject>
<subj-group><subject>Protein Folding</subject>
</subj-group>
</subj-group>
<subj-group><subject>Proteomics</subject>
<subj-group><subject>Protein Abundance</subject>
<subject>Protein Interactions</subject>
<subject>Spectrometric Identification of Proteins</subject>
</subj-group>
</subj-group>
</subj-group>
</article-categories>
<title-group><article-title>Qualitative and Quantitative Multiplexed Proteomic Analysis of Complex Yeast Protein Fractions That Modulate the Assembly of the Yeast Prion Sup35p</article-title>
<alt-title alt-title-type="running-head">Proteomic Analysis of [<italic>PSI</italic>
<sup>+</sup>
]</alt-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Redeker</surname>
<given-names>Virginie</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hughes</surname>
<given-names>Chris</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Savistchenko</surname>
<given-names>Jimmy</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Vissers</surname>
<given-names>Johannes P. C.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Melki</surname>
<given-names>Ronald</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1"><label>1</label>
<addr-line>Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France</addr-line>
</aff>
<aff id="aff2"><label>2</label>
<addr-line>Waters Corporation, Atlas Park, Manchester, United Kingdom</addr-line>
</aff>
<contrib-group><contrib contrib-type="editor"><name><surname>Zheng</surname>
<given-names>Jie</given-names>
</name>
<role>Editor</role>
<xref ref-type="aff" rid="edit1"></xref>
</contrib>
</contrib-group>
<aff id="edit1">University of Akron, United States of America</aff>
<author-notes><corresp id="cor1">* E-mail: <email>redeker@lebs.cnrs-gif.fr</email>
(VR); <email>melki@lebs.cnrs-gif.fr</email>
(RM)</corresp>
<fn fn-type="con"><p>Conceived and designed the experiments: VR RM. Performed the experiments: VR JS CH. Analyzed the data: VR JPCV RM. Contributed reagents/materials/analysis tools: JS JPCV. Wrote the paper: VR RM.</p>
</fn>
</author-notes>
<pub-date pub-type="collection"><year>2011</year>
</pub-date>
<pub-date pub-type="epub"><day>13</day>
<month>9</month>
<year>2011</year>
</pub-date>
<volume>6</volume>
<issue>9</issue>
<elocation-id>e23659</elocation-id>
<history><date date-type="received"><day>15</day>
<month>4</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>22</day>
<month>7</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>Redeker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement>
<copyright-year>2011</copyright-year>
</permissions>
<abstract><sec><title>Background</title>
<p>The aggregation of the baker's yeast prion Sup35p is at the origin of the transmissible [<italic>PSI<sup>+</sup>
</italic>
] trait. We and others have shown that molecular chaperones modulate Sup35p aggregation. However, other protein classes might be involved in [<italic>PSI<sup>+</sup>
</italic>
] formation.</p>
</sec>
<sec><title>Results</title>
<p>We designed a functional proteomic study that combines two techniques to identify modulators of Sup35p aggregation and describe the changes associated to [<italic>PSI<sup>+</sup>
</italic>
] formation. The first allows measuring the effect of fractionated <italic>Saccharomyces cerevisiae</italic>
cytosolic extracts from [<italic>PSI<sup>+</sup>
</italic>
] and [<italic>psi<sup>−</sup>
</italic>
] yeast cells on Sup35p assembly. The second is a multiplex qualitative and quantitative comparison of protein composition of active and inactive fractions using a gel-free and label-free LC-MS approach. We identify changes in proteins involved in translation, folding, degradation, oxido-reduction and metabolic processes.</p>
</sec>
<sec><title>Conclusion</title>
<p>Our functional proteomic study provides the first inventory list of over 300 proteins that directly or indirectly affect Sup35p aggregation and [<italic>PSI<sup>+</sup>
</italic>
] formation. Our results highlight the complexity of the cellular changes accompanying [<italic>PSI<sup>+</sup>
</italic>
] formation and pave the way for <italic>in vitro</italic>
studies aimed to document the effect of individual and/or combinations of proteins identified here, susceptible of affecting Sup35p assembly.</p>
</sec>
</abstract>
<counts><page-count count="11"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>France</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Grand Manchester</li>
<li>Île-de-France</li>
</region>
<settlement><li>Gif-sur-Yvette</li>
<li>Manchester</li>
</settlement>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Redeker, Virginie" sort="Redeker, Virginie" uniqKey="Redeker V" first="Virginie" last="Redeker">Virginie Redeker</name>
</region>
<name sortKey="Melki, Ronald" sort="Melki, Ronald" uniqKey="Melki R" first="Ronald" last="Melki">Ronald Melki</name>
<name sortKey="Savistchenko, Jimmy" sort="Savistchenko, Jimmy" uniqKey="Savistchenko J" first="Jimmy" last="Savistchenko">Jimmy Savistchenko</name>
</country>
<country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Hughes, Chris" sort="Hughes, Chris" uniqKey="Hughes C" first="Chris" last="Hughes">Chris Hughes</name>
</region>
<name sortKey="Vissers, Johannes P C" sort="Vissers, Johannes P C" uniqKey="Vissers J" first="Johannes P. C." last="Vissers">Johannes P. C. Vissers</name>
</country>
</tree>
</affiliations>
</record>
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