Septins Regulate Bacterial Entry into Host Cells
Identifieur interne : 000947 ( Ncbi/Merge ); précédent : 000946; suivant : 000948Septins Regulate Bacterial Entry into Host Cells
Auteurs : Serge Mostowy [France] ; To Nam Tham [France] ; Anne Danckaert [France] ; Stéphanie Guadagnini [France] ; Stéphanie Boisson-Dupuis [France] ; Javier Pizarro-Cerdá [France] ; Pascale Cossart [France]Source :
- PLoS ONE [ 1932-6203 ] ; 2009.
Abstract
Septins are conserved GTPases that form filaments and are required in many organisms for several processes including cytokinesis. We previously identified SEPT9 associated with phagosomes containing latex beads coated with the
Here, we investigated septin function during entry of invasive bacteria in non-phagocytic mammalian cells. We found that SEPT9, and its interacting partners SEPT2 and SEPT11, are recruited as collars next to actin at the site of entry of
Together these findings highlight novel roles for SEPT2, and distinguish the roles of septin and actin in bacterial entry.
Url:
DOI: 10.1371/journal.pone.0004196
PubMed: 19145258
PubMed Central: 2626286
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Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U604, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>INRA, USC2020, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INRA, USC2020, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Nam Tham, To" sort="Nam Tham, To" uniqKey="Nam Tham T" first="To" last="Nam Tham">To Nam Tham</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Inserm, U604, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U604, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>INRA, USC2020, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INRA, USC2020, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Danckaert, Anne" sort="Danckaert, Anne" uniqKey="Danckaert A" first="Anne" last="Danckaert">Anne Danckaert</name><affiliation wicri:level="3"><nlm:aff id="aff4"><addr-line>Institut Pasteur, Plate-forme d'Imagerie Dynamique, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Plate-forme d'Imagerie Dynamique, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Guadagnini, Stephanie" sort="Guadagnini, Stephanie" uniqKey="Guadagnini S" first="Stéphanie" last="Guadagnini">Stéphanie Guadagnini</name><affiliation wicri:level="3"><nlm:aff id="aff5"><addr-line>Institut Pasteur, Plateforme de Microscopie Electronique, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Plateforme de Microscopie Electronique, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Boisson Dupuis, Stephanie" sort="Boisson Dupuis, Stephanie" uniqKey="Boisson Dupuis S" first="Stéphanie" last="Boisson-Dupuis">Stéphanie Boisson-Dupuis</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Inserm, U604, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U604, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>INRA, USC2020, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INRA, USC2020, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pizarro Cerda, Javier" sort="Pizarro Cerda, Javier" uniqKey="Pizarro Cerda J" first="Javier" last="Pizarro-Cerdá">Javier Pizarro-Cerdá</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Inserm, U604, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U604, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>INRA, USC2020, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INRA, USC2020, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Cossart, Pascale" sort="Cossart, Pascale" uniqKey="Cossart P" first="Pascale" last="Cossart">Pascale Cossart</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Inserm, U604, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Inserm, U604, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>INRA, USC2020, Paris, France</addr-line></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>INRA, USC2020, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Septins are conserved GTPases that form filaments and are required in many organisms for several processes including cytokinesis. We previously identified SEPT9 associated with phagosomes containing latex beads coated with the <italic>Listeria</italic> surface protein InlB.</p></sec><sec><title>Methodology/Principal Findings</title><p>Here, we investigated septin function during entry of invasive bacteria in non-phagocytic mammalian cells. We found that SEPT9, and its interacting partners SEPT2 and SEPT11, are recruited as collars next to actin at the site of entry of <italic>Listeria</italic> and <italic>Shigella</italic>. SEPT2-depletion by siRNA decreased bacterial invasion, suggesting that septins have roles during particle entry. Incubating cells with InlB-coated beads confirmed an essential role for SEPT2. Moreover, SEPT2-depletion impaired InlB-mediated stimulation of Met-dependent signaling as shown by FRET.</p></sec><sec><title>Conclusions/Significance</title><p>Together these findings highlight novel roles for SEPT2, and distinguish the roles of septin and actin in bacterial entry.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title>PLoS ONE</journal-title><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19145258</article-id><article-id pub-id-type="pmc">2626286</article-id><article-id pub-id-type="publisher-id">08-PONE-RA-07325R1</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0004196</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline"><subject>Cell Biology/Cytoskeleton</subject><subject>Cell Biology/Membranes and Sorting</subject><subject>Infectious Diseases/Bacterial Infections</subject></subj-group></article-categories><title-group><article-title>Septins Regulate Bacterial Entry into Host Cells</article-title><alt-title alt-title-type="running-head">Septins and Bacterial Invasion</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mostowy</surname><given-names>Serge</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nam Tham</surname><given-names>To</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Danckaert</surname><given-names>Anne</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Guadagnini</surname><given-names>Stéphanie</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Boisson-Dupuis</surname><given-names>Stéphanie</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Pizarro-Cerdá</surname><given-names>Javier</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Cossart</surname><given-names>Pascale</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>Institut Pasteur, Unité des Interactions Bactéries-Cellules, Paris, France</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Inserm, U604, Paris, France</addr-line></aff><aff id="aff3"><label>3</label><addr-line>INRA, USC2020, Paris, France</addr-line></aff><aff id="aff4"><label>4</label><addr-line>Institut Pasteur, Plate-forme d'Imagerie Dynamique, Paris, France</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Institut Pasteur, Plateforme de Microscopie Electronique, Paris, France</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Valdivia</surname><given-names>Raphael H.</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1">Duke University Medical Center, United States of America</aff><author-notes><corresp id="cor1">* E-mail: <email>pcossart@pasteur.fr</email></corresp><fn fn-type="con"><p>Conceived and designed the experiments: SM JPC PC. Performed the experiments: SM TNT SG. Analyzed the data: SM AD. Contributed reagents/materials/analysis tools: SM SBD. Wrote the paper: SM JPC PC.</p></fn></author-notes><pub-date pub-type="collection"><year>2009</year></pub-date><pub-date pub-type="epub"><day>15</day><month>1</month><year>2009</year></pub-date><volume>4</volume><issue>1</issue><elocation-id>e4196</elocation-id><history><date date-type="received"><day>14</day><month>11</month><year>2008</year></date><date date-type="accepted"><day>5</day><month>12</month><year>2008</year></date></history><copyright-statement>Mostowy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</copyright-statement><copyright-year>2009</copyright-year><abstract><sec><title>Background</title><p>Septins are conserved GTPases that form filaments and are required in many organisms for several processes including cytokinesis. We previously identified SEPT9 associated with phagosomes containing latex beads coated with the <italic>Listeria</italic> surface protein InlB.</p></sec><sec><title>Methodology/Principal Findings</title><p>Here, we investigated septin function during entry of invasive bacteria in non-phagocytic mammalian cells. We found that SEPT9, and its interacting partners SEPT2 and SEPT11, are recruited as collars next to actin at the site of entry of <italic>Listeria</italic> and <italic>Shigella</italic>. SEPT2-depletion by siRNA decreased bacterial invasion, suggesting that septins have roles during particle entry. Incubating cells with InlB-coated beads confirmed an essential role for SEPT2. Moreover, SEPT2-depletion impaired InlB-mediated stimulation of Met-dependent signaling as shown by FRET.</p></sec><sec><title>Conclusions/Significance</title><p>Together these findings highlight novel roles for SEPT2, and distinguish the roles of septin and actin in bacterial entry.</p></sec></abstract><counts><page-count count="11"></page-count></counts></article-meta></front></pmc><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Mostowy, Serge" sort="Mostowy, Serge" uniqKey="Mostowy S" first="Serge" last="Mostowy">Serge Mostowy</name></region><name sortKey="Boisson Dupuis, Stephanie" sort="Boisson Dupuis, Stephanie" uniqKey="Boisson Dupuis S" first="Stéphanie" last="Boisson-Dupuis">Stéphanie Boisson-Dupuis</name><name sortKey="Boisson Dupuis, Stephanie" sort="Boisson Dupuis, Stephanie" uniqKey="Boisson Dupuis S" first="Stéphanie" last="Boisson-Dupuis">Stéphanie Boisson-Dupuis</name><name sortKey="Boisson Dupuis, Stephanie" sort="Boisson Dupuis, Stephanie" uniqKey="Boisson Dupuis S" first="Stéphanie" last="Boisson-Dupuis">Stéphanie Boisson-Dupuis</name><name sortKey="Cossart, Pascale" sort="Cossart, Pascale" uniqKey="Cossart P" first="Pascale" last="Cossart">Pascale Cossart</name><name sortKey="Cossart, Pascale" sort="Cossart, Pascale" uniqKey="Cossart P" first="Pascale" last="Cossart">Pascale Cossart</name><name sortKey="Cossart, Pascale" sort="Cossart, Pascale" uniqKey="Cossart P" first="Pascale" last="Cossart">Pascale Cossart</name><name sortKey="Danckaert, Anne" sort="Danckaert, Anne" uniqKey="Danckaert A" first="Anne" last="Danckaert">Anne Danckaert</name><name sortKey="Guadagnini, Stephanie" sort="Guadagnini, Stephanie" uniqKey="Guadagnini S" first="Stéphanie" last="Guadagnini">Stéphanie Guadagnini</name><name sortKey="Mostowy, Serge" sort="Mostowy, Serge" uniqKey="Mostowy S" first="Serge" last="Mostowy">Serge Mostowy</name><name sortKey="Mostowy, Serge" sort="Mostowy, Serge" uniqKey="Mostowy S" first="Serge" last="Mostowy">Serge Mostowy</name><name sortKey="Nam Tham, To" sort="Nam Tham, To" uniqKey="Nam Tham T" first="To" last="Nam Tham">To Nam Tham</name><name sortKey="Nam Tham, To" sort="Nam Tham, To" uniqKey="Nam Tham T" first="To" last="Nam Tham">To Nam Tham</name><name sortKey="Nam Tham, To" sort="Nam Tham, To" uniqKey="Nam Tham T" first="To" last="Nam Tham">To Nam Tham</name><name sortKey="Pizarro Cerda, Javier" sort="Pizarro Cerda, Javier" uniqKey="Pizarro Cerda J" first="Javier" last="Pizarro-Cerdá">Javier Pizarro-Cerdá</name><name sortKey="Pizarro Cerda, Javier" sort="Pizarro Cerda, Javier" uniqKey="Pizarro Cerda J" first="Javier" last="Pizarro-Cerdá">Javier Pizarro-Cerdá</name><name sortKey="Pizarro Cerda, Javier" sort="Pizarro Cerda, Javier" uniqKey="Pizarro Cerda J" first="Javier" last="Pizarro-Cerdá">Javier Pizarro-Cerdá</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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