Activity-dependent secretion of alpha-synuclein by enteric neurons.
Identifieur interne : 001040 ( Ncbi/Curation ); précédent : 001039; suivant : 001041Activity-dependent secretion of alpha-synuclein by enteric neurons.
Auteurs : Sébastien Paillusson [France] ; Thomas Clairembault ; Mandy Biraud ; Michel Neunlist ; Pascal DerkinderenSource :
- Journal of neurochemistry [ 1471-4159 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Blotting, Western, Brefeldin A (pharmacology), Colforsin (pharmacology), Enteric Nervous System (cytology), Enteric Nervous System (secretion), Exocytosis (physiology), Intestine, Small (innervation), Neurons (cytology), Neurons (drug effects), Neurons (secretion), Parkinson Disease (metabolism), Parkinson Disease (pathology), Parkinson Disease (physiopathology), Primary Cell Culture, Protein Synthesis Inhibitors (pharmacology), Rats, alpha-Synuclein (secretion).
- MESH :
- chemical , pharmacology : Brefeldin A, Colforsin, Protein Synthesis Inhibitors.
- cytology : Enteric Nervous System, Neurons.
- drug effects : Neurons.
- innervation : Intestine, Small.
- metabolism : Parkinson Disease.
- pathology : Parkinson Disease.
- physiology : Exocytosis.
- physiopathology : Parkinson Disease.
- secretion : Enteric Nervous System, Neurons, alpha-Synuclein.
- Animals, Blotting, Western, Primary Cell Culture, Rats.
Abstract
There is growing evidence supporting a role of extracellular alpha-synuclein in the spreading of Parkinson's disease (PD) pathology. Recent pathological studies have raised the possibility that the enteric nervous system (ENS) is one of the initial sites of alpha-synuclein pathology in PD. We therefore undertook this survey to determine whether alpha-synuclein can be secreted by enteric neurons. Alpha-synuclein secretion was assessed by immunoblot analysis of the culture medium from primary culture of ENS. We show that alpha-synuclein is physiologically secreted by enteric neurons via a conventional, endoplasmic reticulum/Golgi-dependent exocytosis, in a neuronal activity-regulated manner. Our study is the first to evidence that enteric neurons are capable of secreting alpha-synuclein, thereby providing new insights into the role of the ENS in the pathophysiology of PD.
DOI: 10.1111/jnc.12131
PubMed: 23278133
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000737
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000705
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000705
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001040
Links to Exploration step
pubmed:23278133Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Activity-dependent secretion of alpha-synuclein by enteric neurons.</title>
<author><name sortKey="Paillusson, Sebastien" sort="Paillusson, Sebastien" uniqKey="Paillusson S" first="Sébastien" last="Paillusson">Sébastien Paillusson</name>
<affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Inserm, U913, Nantes</wicri:regionArea>
<placeName><region type="region">Pays de la Loire</region>
<region type="old region">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Clairembault, Thomas" sort="Clairembault, Thomas" uniqKey="Clairembault T" first="Thomas" last="Clairembault">Thomas Clairembault</name>
</author>
<author><name sortKey="Biraud, Mandy" sort="Biraud, Mandy" uniqKey="Biraud M" first="Mandy" last="Biraud">Mandy Biraud</name>
</author>
<author><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name>
</author>
<author><name sortKey="Derkinderen, Pascal" sort="Derkinderen, Pascal" uniqKey="Derkinderen P" first="Pascal" last="Derkinderen">Pascal Derkinderen</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23278133</idno>
<idno type="pmid">23278133</idno>
<idno type="doi">10.1111/jnc.12131</idno>
<idno type="wicri:Area/PubMed/Corpus">000737</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000737</idno>
<idno type="wicri:Area/PubMed/Curation">000705</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000705</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000705</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000705</idno>
<idno type="wicri:Area/Ncbi/Merge">001040</idno>
<idno type="wicri:Area/Ncbi/Curation">001040</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Activity-dependent secretion of alpha-synuclein by enteric neurons.</title>
<author><name sortKey="Paillusson, Sebastien" sort="Paillusson, Sebastien" uniqKey="Paillusson S" first="Sébastien" last="Paillusson">Sébastien Paillusson</name>
<affiliation wicri:level="3"><nlm:affiliation>Inserm, U913, Nantes, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Inserm, U913, Nantes</wicri:regionArea>
<placeName><region type="region">Pays de la Loire</region>
<region type="old region">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Clairembault, Thomas" sort="Clairembault, Thomas" uniqKey="Clairembault T" first="Thomas" last="Clairembault">Thomas Clairembault</name>
</author>
<author><name sortKey="Biraud, Mandy" sort="Biraud, Mandy" uniqKey="Biraud M" first="Mandy" last="Biraud">Mandy Biraud</name>
</author>
<author><name sortKey="Neunlist, Michel" sort="Neunlist, Michel" uniqKey="Neunlist M" first="Michel" last="Neunlist">Michel Neunlist</name>
</author>
<author><name sortKey="Derkinderen, Pascal" sort="Derkinderen, Pascal" uniqKey="Derkinderen P" first="Pascal" last="Derkinderen">Pascal Derkinderen</name>
</author>
</analytic>
<series><title level="j">Journal of neurochemistry</title>
<idno type="eISSN">1471-4159</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Brefeldin A (pharmacology)</term>
<term>Colforsin (pharmacology)</term>
<term>Enteric Nervous System (cytology)</term>
<term>Enteric Nervous System (secretion)</term>
<term>Exocytosis (physiology)</term>
<term>Intestine, Small (innervation)</term>
<term>Neurons (cytology)</term>
<term>Neurons (drug effects)</term>
<term>Neurons (secretion)</term>
<term>Parkinson Disease (metabolism)</term>
<term>Parkinson Disease (pathology)</term>
<term>Parkinson Disease (physiopathology)</term>
<term>Primary Cell Culture</term>
<term>Protein Synthesis Inhibitors (pharmacology)</term>
<term>Rats</term>
<term>alpha-Synuclein (secretion)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Brefeldin A</term>
<term>Colforsin</term>
<term>Protein Synthesis Inhibitors</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Enteric Nervous System</term>
<term>Neurons</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Neurons</term>
</keywords>
<keywords scheme="MESH" qualifier="innervation" xml:lang="en"><term>Intestine, Small</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Parkinson Disease</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Parkinson Disease</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Exocytosis</term>
</keywords>
<keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Parkinson Disease</term>
</keywords>
<keywords scheme="MESH" qualifier="secretion" xml:lang="en"><term>Enteric Nervous System</term>
<term>Neurons</term>
<term>alpha-Synuclein</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Blotting, Western</term>
<term>Primary Cell Culture</term>
<term>Rats</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">There is growing evidence supporting a role of extracellular alpha-synuclein in the spreading of Parkinson's disease (PD) pathology. Recent pathological studies have raised the possibility that the enteric nervous system (ENS) is one of the initial sites of alpha-synuclein pathology in PD. We therefore undertook this survey to determine whether alpha-synuclein can be secreted by enteric neurons. Alpha-synuclein secretion was assessed by immunoblot analysis of the culture medium from primary culture of ENS. We show that alpha-synuclein is physiologically secreted by enteric neurons via a conventional, endoplasmic reticulum/Golgi-dependent exocytosis, in a neuronal activity-regulated manner. Our study is the first to evidence that enteric neurons are capable of secreting alpha-synuclein, thereby providing new insights into the role of the ENS in the pathophysiology of PD.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001040 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 001040 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:23278133 |texte= Activity-dependent secretion of alpha-synuclein by enteric neurons. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:23278133" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a ParkinsonFranceV1
![]() | This area was generated with Dilib version V0.6.29. | ![]() |