High retinoid X receptor expression in JEG‐3 choriocarcinoma cells: Involvement in cell function modulation by retinoids
Identifieur interne : 004849 ( Main/Merge ); précédent : 004848; suivant : 004850High retinoid X receptor expression in JEG‐3 choriocarcinoma cells: Involvement in cell function modulation by retinoids
Auteurs : Jean Guibourdenche [France] ; Sylvie Roulier [France] ; Cécile Rochette-Egly [France] ; Danièle Evain-Brion [France]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 1998-09.
Abstract
Retinoic acid (RA) is an important mediator of cell differentiation. It stimulates hCG secretion by JEG‐3 choriocarcinoma cells in vitro after a time lag. The first aim of this study was to characterize which types of retinoid receptors (RARs and RXRs) are present in JEG‐3 cells. Using Western blot analysis and immunocytochemistry with specific antibodies as well as Northern blot analysis, we found that JEG‐3 cells expressed RARα and RXRα, the latter being the predominant receptor. We then analyzed the action on cell proliferation and hCG secretion of the physiological retinoids all‐trans RA (RA) and 9 cis RA as well as synthetic retinoids with specific affinity for RARα and RXRα. All these retinoids were potent inhibitors of cell growth, maximal inhibition (72 ± 2%) being observed after 4 days of treatment with Ro 25, a RXRα specific ligand. Within 24 h, 9 cis RA and Ro 25 stimulated hCG secretion, and maximal stimulation (1,472 ± 10%) occurred at 48 h with the RXRα‐specific ligand. The RARα‐specific ligand also stimulated hCG secretion but to a lower extend and after a delay of 48 h. These results suggest a predominant role of RXRα in mediating the biological effects of retinoids on JEG‐3 cells and the possible induction by RA itself of the metabolic pathway leading to 9 cis RA. J. Cell. Physiol. 176:595–601, 1998. © 1998 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/(SICI)1097-4652(199809)176:3<595::AID-JCP16>3.0.CO;2-Z
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001843
- to stream Istex, to step Curation: 001841
- to stream Istex, to step Checkpoint: 001491
Links to Exploration step
ISTEX:111C6403133F551893FB1C5394DE2D584D80118BLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">High retinoid X receptor expression in JEG‐3 choriocarcinoma cells: Involvement in cell function modulation by retinoids</title>
<author><name sortKey="Guibourdenche, Jean" sort="Guibourdenche, Jean" uniqKey="Guibourdenche J" first="Jean" last="Guibourdenche">Jean Guibourdenche</name>
</author>
<author><name sortKey="Roulier, Sylvie" sort="Roulier, Sylvie" uniqKey="Roulier S" first="Sylvie" last="Roulier">Sylvie Roulier</name>
</author>
<author><name sortKey="Rochette Gly, Cecile" sort="Rochette Gly, Cecile" uniqKey="Rochette Gly C" first="Cécile" last="Rochette-Egly">Cécile Rochette-Egly</name>
</author>
<author><name sortKey="Evain Rion, Daniele" sort="Evain Rion, Daniele" uniqKey="Evain Rion D" first="Danièle" last="Evain-Brion">Danièle Evain-Brion</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:111C6403133F551893FB1C5394DE2D584D80118B</idno>
<date when="1998" year="1998">1998</date>
<idno type="doi">10.1002/(SICI)1097-4652(199809)176:3<595::AID-JCP16>3.0.CO;2-Z</idno>
<idno type="url">https://api.istex.fr/document/111C6403133F551893FB1C5394DE2D584D80118B/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001843</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001843</idno>
<idno type="wicri:Area/Istex/Curation">001841</idno>
<idno type="wicri:Area/Istex/Checkpoint">001491</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001491</idno>
<idno type="wicri:doubleKey">0021-9541:1998:Guibourdenche J:high:retinoid:x</idno>
<idno type="wicri:Area/Main/Merge">004849</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">High retinoid X receptor expression in JEG‐3 choriocarcinoma cells: Involvement in cell function modulation by retinoids</title>
<author><name sortKey="Guibourdenche, Jean" sort="Guibourdenche, Jean" uniqKey="Guibourdenche J" first="Jean" last="Guibourdenche">Jean Guibourdenche</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Unité INSERM 427, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Roulier, Sylvie" sort="Roulier, Sylvie" uniqKey="Roulier S" first="Sylvie" last="Roulier">Sylvie Roulier</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Unité INSERM 427, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rochette Gly, Cecile" sort="Rochette Gly, Cecile" uniqKey="Rochette Gly C" first="Cécile" last="Rochette-Egly">Cécile Rochette-Egly</name>
<affiliation wicri:level="1"><country xml:lang="fr">France</country>
<wicri:regionArea>IGBMC, BP 163, CU de Strasbourg</wicri:regionArea>
<wicri:noRegion>CU de Strasbourg</wicri:noRegion>
<wicri:noRegion>CU de Strasbourg</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Evain Rion, Daniele" sort="Evain Rion, Daniele" uniqKey="Evain Rion D" first="Danièle" last="Evain-Brion">Danièle Evain-Brion</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Unité INSERM 427, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Unité INSERM 427, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, 4 Avenue de l'Observatoire, 75006 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Cellular Physiology</title>
<title level="j" type="abbrev">J. Cell. Physiol.</title>
<idno type="ISSN">0021-9541</idno>
<idno type="eISSN">1097-4652</idno>
<imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="1998-09">1998-09</date>
<biblScope unit="volume">176</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="595">595</biblScope>
<biblScope unit="page" to="601">601</biblScope>
</imprint>
<idno type="ISSN">0021-9541</idno>
</series>
<idno type="istex">111C6403133F551893FB1C5394DE2D584D80118B</idno>
<idno type="DOI">10.1002/(SICI)1097-4652(199809)176:3<595::AID-JCP16>3.0.CO;2-Z</idno>
<idno type="ArticleID">JCP16</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0021-9541</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Retinoic acid (RA) is an important mediator of cell differentiation. It stimulates hCG secretion by JEG‐3 choriocarcinoma cells in vitro after a time lag. The first aim of this study was to characterize which types of retinoid receptors (RARs and RXRs) are present in JEG‐3 cells. Using Western blot analysis and immunocytochemistry with specific antibodies as well as Northern blot analysis, we found that JEG‐3 cells expressed RARα and RXRα, the latter being the predominant receptor. We then analyzed the action on cell proliferation and hCG secretion of the physiological retinoids all‐trans RA (RA) and 9 cis RA as well as synthetic retinoids with specific affinity for RARα and RXRα. All these retinoids were potent inhibitors of cell growth, maximal inhibition (72 ± 2%) being observed after 4 days of treatment with Ro 25, a RXRα specific ligand. Within 24 h, 9 cis RA and Ro 25 stimulated hCG secretion, and maximal stimulation (1,472 ± 10%) occurred at 48 h with the RXRα‐specific ligand. The RARα‐specific ligand also stimulated hCG secretion but to a lower extend and after a delay of 48 h. These results suggest a predominant role of RXRα in mediating the biological effects of retinoids on JEG‐3 cells and the possible induction by RA itself of the metabolic pathway leading to 9 cis RA. J. Cell. Physiol. 176:595–601, 1998. © 1998 Wiley‐Liss, Inc.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004849 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004849 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:111C6403133F551893FB1C5394DE2D584D80118B |texte= High retinoid X receptor expression in JEG‐3 choriocarcinoma cells: Involvement in cell function modulation by retinoids }}
This area was generated with Dilib version V0.6.29. |