Efficient Microbial Conversion of L-Tyrosine to L-DOPA by Brevundimonas sp. SGJ
Identifieur interne : 001479 ( Main/Merge ); précédent : 001478; suivant : 001480Efficient Microbial Conversion of L-Tyrosine to L-DOPA by Brevundimonas sp. SGJ
Auteurs : Shripad N. Surwase [Inde] ; Sushama A. Patil [Inde] ; Onkar A. Apine [Inde] ; Jyoti P. Jadhav [Inde]Source :
- Applied biochemistry and biotechnology [ 0273-2289 ] ; 2012.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
L-DOPA (3,4-dihydroxyphenyl-L-alanine), the most widely used drug for the treatment of Parkinson's disease, was produced in buffer using biomass of Brevundimonas sp. SGJ. The effects of enhancers, such as carrageenan, diatomaceous earth, and activated charcoal, on the L-DOPA production were evaluated to obtain the maximum yield. The optimal process conditions found were pH 8, 2 gl-1 cell mass, 2 gl-1 L-tyrosine, 0.04 gl-1 CuSO4, 0.02 gl-1 L-ascorbic acid, 0.5 gl-1 carrageenan, and 40 °C temperature. In addition, repeated use of cells resulted in the highest yield of 3.81 gl-1 (95.2%) of L-DOPA with utilization of 4 gl-1 L-tyrosine, and the highest tyrosinase activity (9,201 Umg-1) was observed at 18 h of incubation. Furthennore, the produced L-DOPA was confirmed by high-performance thin-layer chromatography, high-performance liquid chromatography, and gas chromatography-mass spectroscopy. Kinetic studies showed significant values of Yp/s, Qs, and qs after optimization of the process. Thus, Brevundimonas sp. SGJ could be an eventual new source for large-scale production of L-DOPA.
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Patil</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biotcchnology, Shivaji University</s1><s2>Vidyanagar, Kolhapur 416004</s2><s3>IND</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Vidyanagar, Kolhapur 416004</wicri:noRegion></affiliation></author><author><name sortKey="Apine, Onkar A" sort="Apine, Onkar A" uniqKey="Apine O" first="Onkar A." last="Apine">Onkar A. Apine</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biotcchnology, Shivaji University</s1><s2>Vidyanagar, Kolhapur 416004</s2><s3>IND</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Vidyanagar, Kolhapur 416004</wicri:noRegion></affiliation></author><author><name sortKey="Jadhav, Jyoti P" sort="Jadhav, Jyoti P" uniqKey="Jadhav J" first="Jyoti P." last="Jadhav">Jyoti P. 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SGJ</title><author><name sortKey="Surwase, Shripad N" sort="Surwase, Shripad N" uniqKey="Surwase S" first="Shripad N." last="Surwase">Shripad N. Surwase</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Shivaji Univcrsity</s1><s2>Kolhapur 416004</s2><s3>IND</s3><sZ>1 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Kolhapur 416004</wicri:noRegion></affiliation></author><author><name sortKey="Patil, Sushama A" sort="Patil, Sushama A" uniqKey="Patil S" first="Sushama A." last="Patil">Sushama A. Patil</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biotcchnology, Shivaji University</s1><s2>Vidyanagar, Kolhapur 416004</s2><s3>IND</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Vidyanagar, Kolhapur 416004</wicri:noRegion></affiliation></author><author><name sortKey="Apine, Onkar A" sort="Apine, Onkar A" uniqKey="Apine O" first="Onkar A." last="Apine">Onkar A. Apine</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biotcchnology, Shivaji University</s1><s2>Vidyanagar, Kolhapur 416004</s2><s3>IND</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Vidyanagar, Kolhapur 416004</wicri:noRegion></affiliation></author><author><name sortKey="Jadhav, Jyoti P" sort="Jadhav, Jyoti P" uniqKey="Jadhav J" first="Jyoti P." last="Jadhav">Jyoti P. Jadhav</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biotcchnology, Shivaji University</s1><s2>Vidyanagar, Kolhapur 416004</s2><s3>IND</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Inde</country><wicri:noRegion>Vidyanagar, Kolhapur 416004</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Applied biochemistry and biotechnology</title><title level="j" type="abbreviated">Appl. biochem. biotechnol.</title><idno type="ISSN">0273-2289</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Applied biochemistry and biotechnology</title><title level="j" type="abbreviated">Appl. biochem. biotechnol.</title><idno type="ISSN">0273-2289</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biotransformation</term><term>Brevundimonas</term><term>Carrageenan</term><term>Levodopa</term><term>Monophenol monooxygenase</term><term>Tyrosine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Biotransformation</term><term>Tyrosine</term><term>Lévodopa</term><term>Brevundimonas</term><term>Carraghénane</term><term>Monophenol monooxygenase</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">L-DOPA (3,4-dihydroxyphenyl-L-alanine), the most widely used drug for the treatment of Parkinson's disease, was produced in buffer using biomass of Brevundimonas sp. SGJ. The effects of enhancers, such as carrageenan, diatomaceous earth, and activated charcoal, on the <sub>L</sub>-DOPA production were evaluated to obtain the maximum yield. The optimal process conditions found were pH 8, 2 gl<sup>-1</sup> cell mass, 2 gl<sup>-1</sup> <sub>L</sub>-tyrosine, 0.04 gl<sup>-1</sup> CuSO<sub>4</sub>, 0.02 gl<sup>-1</sup> <sub>L</sub>-ascorbic acid, 0.5 gl<sup>-1</sup> carrageenan, and 40 °C temperature. In addition, repeated use of cells resulted in the highest yield of 3.81 gl<sup>-1</sup> (95.2%) of L-DOPA with utilization of 4 gl<sup>-1</sup> <sub>L</sub>-tyrosine, and the highest tyrosinase activity (9,201 Umg<sup>-1</sup>) was observed at 18 h of incubation. Furthennore, the produced L-DOPA was confirmed by high-performance thin-layer chromatography, high-performance liquid chromatography, and gas chromatography-mass spectroscopy. Kinetic studies showed significant values of Y<sub>p/s</sub>, Q<sub>s</sub>, and q<sub>s</sub> after optimization of the process. Thus, Brevundimonas sp. SGJ could be an eventual new source for large-scale production of <sub>L</sub>-DOPA.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Surwase, Shripad N" sort="Surwase, Shripad N" uniqKey="Surwase S" first="Shripad N." last="Surwase">Shripad N. Surwase</name></noRegion><name sortKey="Apine, Onkar A" sort="Apine, Onkar A" uniqKey="Apine O" first="Onkar A." last="Apine">Onkar A. Apine</name><name sortKey="Jadhav, Jyoti P" sort="Jadhav, Jyoti P" uniqKey="Jadhav J" first="Jyoti P." last="Jadhav">Jyoti P. Jadhav</name><name sortKey="Patil, Sushama A" sort="Patil, Sushama A" uniqKey="Patil S" first="Sushama A." last="Patil">Sushama A. Patil</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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