Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction
Identifieur interne : 000522 ( Main/Exploration ); précédent : 000521; suivant : 000523Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction
Auteurs : Thierry Gauthier ; Aurore Claude-Taupin ; Régis Delage-Mourroux ; Michaël Boyer-Guittaut ; Eric HervouetSource :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
Macroautophagy is a highly regulated intracellular degradation process which has been extensively studied over the last decade. This pathway has been initially described as a non selective process inducing the degradation of parts of the cytoplasm as well as organelles at random. Nevertheless, over the last few years, new research highlighted the existence of a more selective autophagy pathway specifically recruiting some organelles or aggregates to the autophagosomes in order to induce their degradation. These selective autophagy pathways such as aggrephagy, mitophagy, pexophagy or xenophagy, involve the intervention of a cargo, the material to be degraded, cargo adapters, the molecules allowing the recruitment of the cargo to the autophagosome, and the proteins of the ATG8 family which link the cargo adapters to the autophagosome. One of the main questions which now remain is to develop new techniques and protocols able to discriminate between these different types of induced autophagy. In our work, we studied the possibility to use the P-LISA technique, which has been recently developed to study endogenous
Url:
DOI: 10.1371/journal.pone.0128701
PubMed: 26034986
PubMed Central: 4452782
Affiliations:
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Le document en format XML
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Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proximity Ligation <italic>In situ</italic>
Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction</title>
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<front><div type="abstract" xml:lang="en"><p>Macroautophagy is a highly regulated intracellular degradation process which has been extensively studied over the last decade. This pathway has been initially described as a non selective process inducing the degradation of parts of the cytoplasm as well as organelles at random. Nevertheless, over the last few years, new research highlighted the existence of a more selective autophagy pathway specifically recruiting some organelles or aggregates to the autophagosomes in order to induce their degradation. These selective autophagy pathways such as aggrephagy, mitophagy, pexophagy or xenophagy, involve the intervention of a cargo, the material to be degraded, cargo adapters, the molecules allowing the recruitment of the cargo to the autophagosome, and the proteins of the ATG8 family which link the cargo adapters to the autophagosome. One of the main questions which now remain is to develop new techniques and protocols able to discriminate between these different types of induced autophagy. In our work, we studied the possibility to use the P-LISA technique, which has been recently developed to study endogenous <italic>in vivo</italic>
protein interactions, as a new technique to characterize the ATG proteins specifically involved in bulk or selective autophagy. In this manuscript, we indeed demonstrate that this technique allows the study of endogenous ATG protein interactions in cells following autophagy induction, but more interestingly that this technique might be used to characterize the ATG proteins involved in selective autophagy.</p>
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<name sortKey="Delage Mourroux, Regis" sort="Delage Mourroux, Regis" uniqKey="Delage Mourroux R" first="Régis" last="Delage-Mourroux">Régis Delage-Mourroux</name>
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