Human embryonic stem cell technology : large scale cell amplification and differentiation
Identifieur interne : 002B17 ( Main/Exploration ); précédent : 002B16; suivant : 002B18Human embryonic stem cell technology : large scale cell amplification and differentiation
Auteurs : Steve K. W. Oh [Singapour] ; Andre B. H. Choo [Singapour]Source :
- Cytotechnology : (Dordrecht) [ 0920-9069 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme, Cellule souche, Culture.
English descriptors
- KwdEn :
Abstract
Embryonic stem cells (ESC) hold the promise of overcoming many diseases as potential sources of, for example, dopaminergic neural cells for Parkinson's Disease to pancreatic islets to relieve diabetic patients of their daily insulin injections. While an embryo has the innate capacity to develop fully functional differentiated tissues; biologists are finding that it is much more complex to derive singular, pure populations of primary cells from the highly versatile ESC from this embryonic parent. Thus, a substantial investment in developing the technologies to expand and differentiate these cells is required in the next decade to move this promise into reality. In this review we document the current standard assays for characterising human ESC (hESC), the status of 'defined' feeder-free culture conditions for undifferentiated hESC growth, examine the quality controls that will be required to be established for monitoring their growth, review current methods for expansion and differentiation, and speculate on the possible routes of scaling up the differentiation of hESC to therapeutic quantities.
Affiliations:
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Choo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Stem Cell Group, Bioprocessing Technology Institute, 20 Biopolis Way, #06 - 01 Centros</s1><s2>Singapore 138668</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 138668</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0448821</idno><date when="2006">2006</date><idno type="stanalyst">PASCAL 06-0448821 INIST</idno><idno type="RBID">Pascal:06-0448821</idno><idno type="wicri:Area/PascalFrancis/Corpus">000B30</idno><idno type="wicri:Area/PascalFrancis/Curation">000904</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000A02</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000A02</idno><idno type="wicri:doubleKey">0920-9069:2006:Oh S:human:embryonic:stem</idno><idno type="wicri:Area/Main/Merge">002E42</idno><idno type="wicri:Area/Main/Curation">002B17</idno><idno type="wicri:Area/Main/Exploration">002B17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Human embryonic stem cell technology : large scale cell amplification and differentiation</title><author><name sortKey="Oh, Steve K W" sort="Oh, Steve K W" uniqKey="Oh S" first="Steve K. W." last="Oh">Steve K. W. Oh</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Stem Cell Group, Bioprocessing Technology Institute, 20 Biopolis Way, #06 - 01 Centros</s1><s2>Singapore 138668</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 138668</wicri:noRegion></affiliation></author><author><name sortKey="Choo, Andre B H" sort="Choo, Andre B H" uniqKey="Choo A" first="Andre B. H." last="Choo">Andre B. H. Choo</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Stem Cell Group, Bioprocessing Technology Institute, 20 Biopolis Way, #06 - 01 Centros</s1><s2>Singapore 138668</s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore 138668</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Cytotechnology : (Dordrecht)</title><title level="j" type="abbreviated">Cytotechnology : (Dordr.)</title><idno type="ISSN">0920-9069</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Cytotechnology : (Dordrecht)</title><title level="j" type="abbreviated">Cytotechnology : (Dordr.)</title><idno type="ISSN">0920-9069</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplification</term><term>Bioreactor</term><term>Cell differentiation</term><term>Culture</term><term>Dimensioning</term><term>Embryo</term><term>Human</term><term>Serum free medium</term><term>Stem cell</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Homme</term><term>Embryon</term><term>Cellule souche</term><term>Cytodifférenciation</term><term>Amplification</term><term>Bioréacteur</term><term>Dimensionnement</term><term>Milieu sans sérum</term><term>Culture</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term><term>Cellule souche</term><term>Culture</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Embryonic stem cells (ESC) hold the promise of overcoming many diseases as potential sources of, for example, dopaminergic neural cells for Parkinson's Disease to pancreatic islets to relieve diabetic patients of their daily insulin injections. While an embryo has the innate capacity to develop fully functional differentiated tissues; biologists are finding that it is much more complex to derive singular, pure populations of primary cells from the highly versatile ESC from this embryonic parent. Thus, a substantial investment in developing the technologies to expand and differentiate these cells is required in the next decade to move this promise into reality. In this review we document the current standard assays for characterising human ESC (hESC), the status of 'defined' feeder-free culture conditions for undifferentiated hESC growth, examine the quality controls that will be required to be established for monitoring their growth, review current methods for expansion and differentiation, and speculate on the possible routes of scaling up the differentiation of hESC to therapeutic quantities.</div></front></TEI><affiliations><list><country><li>Singapour</li></country></list><tree><country name="Singapour"><noRegion><name sortKey="Oh, Steve K W" sort="Oh, Steve K W" uniqKey="Oh S" first="Steve K. W." last="Oh">Steve K. W. Oh</name></noRegion><name sortKey="Choo, Andre B H" sort="Choo, Andre B H" uniqKey="Choo A" first="Andre B. H." last="Choo">Andre B. H. Choo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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