Hepatitis B surface antigenaemia and alpha‐foetoprotein detection from dried blood spots: applications to field‐based studies and to clinical care in hepatitis B virus endemic areas
Identifieur interne : 002C53 ( Main/Curation ); précédent : 002C52; suivant : 002C54Hepatitis B surface antigenaemia and alpha‐foetoprotein detection from dried blood spots: applications to field‐based studies and to clinical care in hepatitis B virus endemic areas
Auteurs : M. Mendy ; G. D. Kirk [États-Unis] ; M. Van Der Sande ; A. Jeng-Barry ; O. A. Lesi ; P. Hainaut [France] ; O. Sam ; S. Mcconkey ; H. WhittleSource :
- Journal of Viral Hepatitis [ 1352-0504 ] ; 2005-11.
English descriptors
- KwdEn :
Abstract
Summary. In many resource‐limited regions with endemic hepatitis B virus (HBV), there is limited infrastructure to collect, process, transport, and store blood samples for identification of persons with chronic HBV infection or with hepatocellular carcinoma (HCC). We describe the application of a simple technique using commercially available kits for detection of HBV surface antigen (HBsAg) and alpha‐foetoprotein (AFP) in dried blood spots (DBS) collected on filter paper. Study participants included subjects with and without chronic HBV infection and subjects with HCC or cirrhosis. Three to five blood drops were dried on filter paper. Dried blood (equivalent to 20 μL) was eluted and tested for HBsAg by DetermineTM HBsAg and for AFP by counter‐current immuno‐electrophoresis and radio‐immunoassay (RIA). The primary analysis focused on comparison of DBS results to serum testing results as the gold standard. The sensitivity of DBS for detecting chronic HBV infection was 96% (98–98) with specificity of 100% (CI 99–100). Sensitivity of DBS in detecting AFP compared with serum RIA was 73% (60–86) with specificity of 90% (81–98). Both HBsAg and AFP recovery were unaffected when DBS were left at room temperature (30–33 °C) and under humid conditions for up to 28 days prior to elution. We conclude that DBS can be reliably used as an economical and logical alternative for detection of HBsAg in chronically infected patients and for AFP‐based diagnosis of HCC in clinical situations which preclude adequate collection and processing of blood samples. Both research‐oriented field studies and routine clinical care may benefit from application of these techniques in resource‐limited settings.
Url:
DOI: 10.1111/j.1365-2893.2005.00641.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001886
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :001884
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000B16
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :003024
Links to Exploration step
ISTEX:F410C296E566C1900A57895548EF10CDCC25945ECuration
No country items
M. Mendy<affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Hepatitis B surface antigenaemia and alpha‐foetoprotein detection from dried blood spots: applications to field‐based studies and to clinical care in hepatitis B virus endemic areas</title><author><name sortKey="Mendy, M" sort="Mendy, M" uniqKey="Mendy M" first="M." last="Mendy">M. Mendy</name></author><author><name sortKey="Kirk, G D" sort="Kirk, G D" uniqKey="Kirk G" first="G. D." last="Kirk">G. D. Kirk</name></author><author><name sortKey="Van Der Sande, M" sort="Van Der Sande, M" uniqKey="Van Der Sande M" first="M." last="Van Der Sande">M. Van Der Sande</name></author><author><name sortKey="Jeng Arry, A" sort="Jeng Arry, A" uniqKey="Jeng Arry A" first="A." last="Jeng-Barry">A. Jeng-Barry</name></author><author><name sortKey="Lesi, O A" sort="Lesi, O A" uniqKey="Lesi O" first="O. A." last="Lesi">O. A. Lesi</name></author><author><name sortKey="Hainaut, P" sort="Hainaut, P" uniqKey="Hainaut P" first="P." last="Hainaut">P. Hainaut</name></author><author><name sortKey="Sam, O" sort="Sam, O" uniqKey="Sam O" first="O." last="Sam">O. Sam</name></author><author><name sortKey="Mcconkey, S" sort="Mcconkey, S" uniqKey="Mcconkey S" first="S." last="Mcconkey">S. Mcconkey</name></author><author><name sortKey="Whittle, H" sort="Whittle, H" uniqKey="Whittle H" first="H." last="Whittle">H. Whittle</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F410C296E566C1900A57895548EF10CDCC25945E</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1111/j.1365-2893.2005.00641.x</idno><idno type="url">https://api.istex.fr/document/F410C296E566C1900A57895548EF10CDCC25945E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001886</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001886</idno><idno type="wicri:Area/Istex/Curation">001884</idno><idno type="wicri:Area/Istex/Checkpoint">000B16</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000B16</idno><idno type="wicri:doubleKey">1352-0504:2005:Mendy M:hepatitis:b:surface</idno><idno type="wicri:Area/Main/Merge">003024</idno><idno type="wicri:Area/Main/Curation">002C53</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Hepatitis B surface antigenaemia and alpha‐foetoprotein detection from dried blood spots: applications to field‐based studies and to clinical care in hepatitis B virus endemic areas</title><author><name sortKey="Mendy, M" sort="Mendy, M" uniqKey="Mendy M" first="M." last="Mendy">M. Mendy</name><affiliation></affiliation><affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation></author><author><name sortKey="Kirk, G D" sort="Kirk, G D" uniqKey="Kirk G" first="G. D." last="Kirk">G. D. Kirk</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Viral Epidemiology Branch, National Cancer Institute, NIH/DHHS, Bethesda, MD</wicri:regionArea><wicri:noRegion>MD</wicri:noRegion></affiliation></author><author><name sortKey="Van Der Sande, M" sort="Van Der Sande, M" uniqKey="Van Der Sande M" first="M." last="Van Der Sande">M. Van Der Sande</name><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation></author><author><name sortKey="Jeng Arry, A" sort="Jeng Arry, A" uniqKey="Jeng Arry A" first="A." last="Jeng-Barry">A. Jeng-Barry</name><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation></author><author><name sortKey="Lesi, O A" sort="Lesi, O A" uniqKey="Lesi O" first="O. A." last="Lesi">O. A. Lesi</name><affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation></author><author><name sortKey="Hainaut, P" sort="Hainaut, P" uniqKey="Hainaut P" first="P." last="Hainaut">P. Hainaut</name><affiliation></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>International Agency for Research on Cancer (IARC), Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Sam, O" sort="Sam, O" uniqKey="Sam O" first="O." last="Sam">O. Sam</name><affiliation><wicri:noCountry code="subField">Africa</wicri:noCountry></affiliation></author><author><name sortKey="Mcconkey, S" sort="Mcconkey, S" uniqKey="Mcconkey S" first="S." last="Mcconkey">S. Mcconkey</name><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation></author><author><name sortKey="Whittle, H" sort="Whittle, H" uniqKey="Whittle H" first="H." last="Whittle">H. Whittle</name><affiliation><wicri:noCountry code="subField">Banjul</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Viral Hepatitis</title><idno type="ISSN">1352-0504</idno><idno type="eISSN">1365-2893</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2005-11">2005-11</date><biblScope unit="volume">12</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="642">642</biblScope><biblScope unit="page" to="647">647</biblScope></imprint><idno type="ISSN">1352-0504</idno></series><idno type="istex">F410C296E566C1900A57895548EF10CDCC25945E</idno><idno type="DOI">10.1111/j.1365-2893.2005.00641.x</idno><idno type="ArticleID">JVH641</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1352-0504</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>alpha‐foetoprotein</term><term>chronic hepatitis B virus infection</term><term>dried blood spot</term><term>hepatitis B surface antigen</term><term>hepatocellular carcinoma</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Summary. In many resource‐limited regions with endemic hepatitis B virus (HBV), there is limited infrastructure to collect, process, transport, and store blood samples for identification of persons with chronic HBV infection or with hepatocellular carcinoma (HCC). We describe the application of a simple technique using commercially available kits for detection of HBV surface antigen (HBsAg) and alpha‐foetoprotein (AFP) in dried blood spots (DBS) collected on filter paper. Study participants included subjects with and without chronic HBV infection and subjects with HCC or cirrhosis. Three to five blood drops were dried on filter paper. Dried blood (equivalent to 20 μL) was eluted and tested for HBsAg by DetermineTM HBsAg and for AFP by counter‐current immuno‐electrophoresis and radio‐immunoassay (RIA). The primary analysis focused on comparison of DBS results to serum testing results as the gold standard. The sensitivity of DBS for detecting chronic HBV infection was 96% (98–98) with specificity of 100% (CI 99–100). Sensitivity of DBS in detecting AFP compared with serum RIA was 73% (60–86) with specificity of 90% (81–98). Both HBsAg and AFP recovery were unaffected when DBS were left at room temperature (30–33 °C) and under humid conditions for up to 28 days prior to elution. We conclude that DBS can be reliably used as an economical and logical alternative for detection of HBsAg in chronically infected patients and for AFP‐based diagnosis of HCC in clinical situations which preclude adequate collection and processing of blood samples. Both research‐oriented field studies and routine clinical care may benefit from application of these techniques in resource‐limited settings.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C53 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002C53 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonFranceV1
|flux= Main
|étape= Curation
|type= RBID
|clé= ISTEX:F410C296E566C1900A57895548EF10CDCC25945E
|texte= Hepatitis B surface antigenaemia and alpha‐foetoprotein detection from dried blood spots: applications to field‐based studies and to clinical care in hepatitis B virus endemic areas
}}
| This area was generated with Dilib version V0.6.29. |  |