Functional melanocytes derived from human pluripotent stem cells engraft into pluristratified epidermis
Identifieur interne : 001578 ( Main/Curation ); précédent : 001577; suivant : 001579Functional melanocytes derived from human pluripotent stem cells engraft into pluristratified epidermis
Auteurs : Xavier Nissan [France] ; Lionel Larribere [France] ; Manoubia Saidani [France] ; Ilse Hurbain [France] ; Cédric Delevoye [France] ; Jessica Feteira [France] ; Gilles Lemaitre [France] ; Marc Peschanski [France] ; Christine Baldeschi [France]Source :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2011.
Abstract
Melanocytes are essential for skin homeostasis and protection, and their defects in humans lead to a wide array of diseases that are potentially extremely severe. To date, the analysis of molecular mechanisms and the function of human melanocytes have been limited because of the difficulties in accessing large numbers of cells with the specific phenotypes. This issue can now be addressed via a differentiation protocol that allows melanocytes to be obtained from pluripotent stem cell lines, either induced or of embryonic origin, based on the use of moderate concentrations of a single cytokine, bone morphogenic protein 4. Human melanocytes derived from pluripotent stem cells exhibit all the characteristic features of their adult counterparts. This includes the enzymatic machinery required for the production and functional delivery of melanin to keratinocytes. Melanocytes also integrate appropriately into organotypic epidermis reconstructed in vitro. The availability of human cells committed to the melanocytic lineage in vitro will enable the investigation of those mechanisms that guide the developmental processes and will facilitate analysis of the molecular mechanisms responsible for genetic diseases. Access to an unlimited resource may also prove a vital tool for the treatment of hypopigmentation disorders when donors with matching haplotypes become available in clinically relevant banks of pluripotent stem cell lines.
Url:
DOI: 10.1073/pnas.1019070108
PubMed: 21856949
PubMed Central: 3169131
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wicri:level="1"><nlm:aff id="aff6">University Evry Val d’Essonne U-861, I-Stem,<institution>Association Française Contre les Myopathies</institution>, 91030 Evry cedex,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Peschanski, Marc" sort="Peschanski, Marc" uniqKey="Peschanski M" first="Marc" last="Peschanski">Marc Peschanski</name><affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff5">Institut National de la Santé et de la Recherche Médicale U-861, I-Stem,<institution>Association Française Contre les Myopathies</institution>, 91030 Evry cedex,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">University Evry Val d’Essonne U-861, I-Stem,<institution>Association Française Contre les Myopathies</institution>, 91030 Evry cedex,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Baldeschi, Christine" sort="Baldeschi, Christine" uniqKey="Baldeschi C" first="Christine" last="Baldeschi">Christine Baldeschi</name><affiliation wicri:level="1"><nlm:aff wicri:cut="; and" id="aff5">Institut National de la Santé et de la Recherche Médicale U-861, I-Stem,<institution>Association Française Contre les Myopathies</institution>, 91030 Evry cedex,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">University Evry Val d’Essonne U-861, I-Stem,<institution>Association Française Contre les Myopathies</institution>, 91030 Evry cedex,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Melanocytes are essential for skin homeostasis and protection, and their defects in humans lead to a wide array of diseases that are potentially extremely severe. To date, the analysis of molecular mechanisms and the function of human melanocytes have been limited because of the difficulties in accessing large numbers of cells with the specific phenotypes. This issue can now be addressed via a differentiation protocol that allows melanocytes to be obtained from pluripotent stem cell lines, either induced or of embryonic origin, based on the use of moderate concentrations of a single cytokine, bone morphogenic protein 4. Human melanocytes derived from pluripotent stem cells exhibit all the characteristic features of their adult counterparts. This includes the enzymatic machinery required for the production and functional delivery of melanin to keratinocytes. Melanocytes also integrate appropriately into organotypic epidermis reconstructed in vitro. The availability of human cells committed to the melanocytic lineage in vitro will enable the investigation of those mechanisms that guide the developmental processes and will facilitate analysis of the molecular mechanisms responsible for genetic diseases. Access to an unlimited resource may also prove a vital tool for the treatment of hypopigmentation disorders when donors with matching haplotypes become available in clinically relevant banks of pluripotent stem cell lines.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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