Copper brain protein protection against free radical-induced neuronal death: Survival ratio in SH-SY5Y neuroblastoma cell cultures.
Identifieur interne : 000042 ( Main/Curation ); précédent : 000041; suivant : 000043Copper brain protein protection against free radical-induced neuronal death: Survival ratio in SH-SY5Y neuroblastoma cell cultures.
Auteurs : Roger Deloncle [France] ; Bernard Fauconneau [France] ; Olivier Guillard [France] ; José Delaval [France] ; Gérard Lesage [France] ; Alain Pineau [France]Source :
- Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [ 1878-3252 ] ; 2017.
Abstract
In Creutzfeldt Jakob, Alzheimer and Parkinson diseases, copper metalloproteins such as prion, amyloid protein precursor and α-synuclein are able to protect against free radicals by reduction from cupric Cu(+2) to cupreous Cu(+). In these pathologies, a regional copper (Cu) brain decrease correlated with an iron, zinc or manganese (Mn) increase has previously been observed, leading to local neuronal death and abnormal deposition of these metalloproteins in β-sheet structures. In this study we demonstrate the protective effect of Cu metalloproteins against deleterious free-radical effects. With neuroblastoma SH-SY5Y cell cultures, we show that bovine brain prion protein in Cu but not Mn form prevents free radical-induced neuronal death. The survival ratio of SH-SY5Y cells has been measured after UV irradiation (free radical production), when the incubating medium is supplemented with bovine brain homogenate in native, Cu or Mn forms. This ratio, about 28% without any addition or with bovine brain protein added in Mn form, increases by as much as 54.73% with addition to the culture medium of native bovine brain protein and by as much as 95.95% if the addition is carried out in cupric form. This protective effect of brain copper protein against free radical-induced neuronal death has been confirmed with Inductively Coupled Plasma Mass Spectrometry Mn and Cu measurement in bovine brain homogenates: respectively lower than detection limit and 9.01μg/g dry weight for native form; lower than detection limit and 825.85μg/g dry weight for Cu-supplemented form and 1.75 and 68.1μg/g dry weight in Mn-supplemented brain homogenate.
DOI: 10.1016/j.jtemb.2016.07.006
PubMed: 27908423
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000034
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000034
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000034
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001B71
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :001B71
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001B71
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000042
Links to Exploration step
pubmed:27908423Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Copper brain protein protection against free radical-induced neuronal death: Survival ratio in SH-SY5Y neuroblastoma cell cultures.</title>
<author><name sortKey="Deloncle, Roger" sort="Deloncle, Roger" uniqKey="Deloncle R" first="Roger" last="Deloncle">Roger Deloncle</name>
<affiliation wicri:level="4"><nlm:affiliation>Université François Rabelais de Tours, Toxicology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours, France. Electronic address: roger.deloncle@univ-tours.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université François Rabelais de Tours, Toxicology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
<orgName type="university">Université François-Rabelais de Tours</orgName>
</affiliation>
</author>
<author><name sortKey="Fauconneau, Bernard" sort="Fauconneau, Bernard" uniqKey="Fauconneau B" first="Bernard" last="Fauconneau">Bernard Fauconneau</name>
<affiliation wicri:level="3"><nlm:affiliation>GREVIC, EA 3808, Biology and Health Pole, 86022 Poitiers, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GREVIC, EA 3808, Biology and Health Pole, 86022 Poitiers</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">Poitiers</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guillard, Olivier" sort="Guillard, Olivier" uniqKey="Guillard O" first="Olivier" last="Guillard">Olivier Guillard</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Poitiers, Faculty of Medicine and Pharmacy, 86000 Poitiers, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Poitiers, Faculty of Medicine and Pharmacy, 86000 Poitiers</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">Poitiers</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Delaval, Jose" sort="Delaval, Jose" uniqKey="Delaval J" first="José" last="Delaval">José Delaval</name>
<affiliation wicri:level="3"><nlm:affiliation>Touraine Laboratory, Indre et Loire General Council, BP 67357- 37073 Tours Cedex 02, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Touraine Laboratory, Indre et Loire General Council, BP 67357- 37073 Tours Cedex 02</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Alsace (région administrative)</region>
<settlement type="city">37073 Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lesage, Gerard" sort="Lesage, Gerard" uniqKey="Lesage G" first="Gérard" last="Lesage">Gérard Lesage</name>
<affiliation wicri:level="4"><nlm:affiliation>Université François Rabelais de Tours, Virology-Immunology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université François Rabelais de Tours, Virology-Immunology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
<orgName type="university">Université François-Rabelais de Tours</orgName>
</affiliation>
</author>
<author><name sortKey="Pineau, Alain" sort="Pineau, Alain" uniqKey="Pineau A" first="Alain" last="Pineau">Alain Pineau</name>
<affiliation wicri:level="4"><nlm:affiliation>Université de Nantes, Toxicology Laboratory Faculty of Pharmacy, 9 Rue Bias, BP 53508- 44035 Nantes Cedex 1, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Nantes, Toxicology Laboratory Faculty of Pharmacy, 9 Rue Bias, BP 53508- 44035 Nantes Cedex 1</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">44035 Nantes</settlement>
</placeName>
<orgName type="university">Université de Nantes</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:27908423</idno>
<idno type="pmid">27908423</idno>
<idno type="doi">10.1016/j.jtemb.2016.07.006</idno>
<idno type="wicri:Area/PubMed/Corpus">000034</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000034</idno>
<idno type="wicri:Area/PubMed/Curation">000034</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000034</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000034</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000034</idno>
<idno type="wicri:Area/Ncbi/Merge">001B71</idno>
<idno type="wicri:Area/Ncbi/Curation">001B71</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001B71</idno>
<idno type="wicri:Area/Main/Merge">000042</idno>
<idno type="wicri:Area/Main/Curation">000042</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Copper brain protein protection against free radical-induced neuronal death: Survival ratio in SH-SY5Y neuroblastoma cell cultures.</title>
<author><name sortKey="Deloncle, Roger" sort="Deloncle, Roger" uniqKey="Deloncle R" first="Roger" last="Deloncle">Roger Deloncle</name>
<affiliation wicri:level="4"><nlm:affiliation>Université François Rabelais de Tours, Toxicology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours, France. Electronic address: roger.deloncle@univ-tours.fr.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université François Rabelais de Tours, Toxicology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
<orgName type="university">Université François-Rabelais de Tours</orgName>
</affiliation>
</author>
<author><name sortKey="Fauconneau, Bernard" sort="Fauconneau, Bernard" uniqKey="Fauconneau B" first="Bernard" last="Fauconneau">Bernard Fauconneau</name>
<affiliation wicri:level="3"><nlm:affiliation>GREVIC, EA 3808, Biology and Health Pole, 86022 Poitiers, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>GREVIC, EA 3808, Biology and Health Pole, 86022 Poitiers</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">Poitiers</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guillard, Olivier" sort="Guillard, Olivier" uniqKey="Guillard O" first="Olivier" last="Guillard">Olivier Guillard</name>
<affiliation wicri:level="3"><nlm:affiliation>University of Poitiers, Faculty of Medicine and Pharmacy, 86000 Poitiers, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>University of Poitiers, Faculty of Medicine and Pharmacy, 86000 Poitiers</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Poitou-Charentes</region>
<settlement type="city">Poitiers</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Delaval, Jose" sort="Delaval, Jose" uniqKey="Delaval J" first="José" last="Delaval">José Delaval</name>
<affiliation wicri:level="3"><nlm:affiliation>Touraine Laboratory, Indre et Loire General Council, BP 67357- 37073 Tours Cedex 02, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Touraine Laboratory, Indre et Loire General Council, BP 67357- 37073 Tours Cedex 02</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Alsace (région administrative)</region>
<settlement type="city">37073 Tours</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lesage, Gerard" sort="Lesage, Gerard" uniqKey="Lesage G" first="Gérard" last="Lesage">Gérard Lesage</name>
<affiliation wicri:level="4"><nlm:affiliation>Université François Rabelais de Tours, Virology-Immunology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université François Rabelais de Tours, Virology-Immunology Laboratory Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours</wicri:regionArea>
<placeName><region type="region" nuts="2">Centre-Val de Loire</region>
<region type="old region" nuts="2">Région Centre</region>
<settlement type="city">Tours</settlement>
</placeName>
<orgName type="university">Université François-Rabelais de Tours</orgName>
</affiliation>
</author>
<author><name sortKey="Pineau, Alain" sort="Pineau, Alain" uniqKey="Pineau A" first="Alain" last="Pineau">Alain Pineau</name>
<affiliation wicri:level="4"><nlm:affiliation>Université de Nantes, Toxicology Laboratory Faculty of Pharmacy, 9 Rue Bias, BP 53508- 44035 Nantes Cedex 1, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Nantes, Toxicology Laboratory Faculty of Pharmacy, 9 Rue Bias, BP 53508- 44035 Nantes Cedex 1</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">44035 Nantes</settlement>
</placeName>
<orgName type="university">Université de Nantes</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)</title>
<idno type="eISSN">1878-3252</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In Creutzfeldt Jakob, Alzheimer and Parkinson diseases, copper metalloproteins such as prion, amyloid protein precursor and α-synuclein are able to protect against free radicals by reduction from cupric Cu(+2) to cupreous Cu(+). In these pathologies, a regional copper (Cu) brain decrease correlated with an iron, zinc or manganese (Mn) increase has previously been observed, leading to local neuronal death and abnormal deposition of these metalloproteins in β-sheet structures. In this study we demonstrate the protective effect of Cu metalloproteins against deleterious free-radical effects. With neuroblastoma SH-SY5Y cell cultures, we show that bovine brain prion protein in Cu but not Mn form prevents free radical-induced neuronal death. The survival ratio of SH-SY5Y cells has been measured after UV irradiation (free radical production), when the incubating medium is supplemented with bovine brain homogenate in native, Cu or Mn forms. This ratio, about 28% without any addition or with bovine brain protein added in Mn form, increases by as much as 54.73% with addition to the culture medium of native bovine brain protein and by as much as 95.95% if the addition is carried out in cupric form. This protective effect of brain copper protein against free radical-induced neuronal death has been confirmed with Inductively Coupled Plasma Mass Spectrometry Mn and Cu measurement in bovine brain homogenates: respectively lower than detection limit and 9.01μg/g dry weight for native form; lower than detection limit and 825.85μg/g dry weight for Cu-supplemented form and 1.75 and 68.1μg/g dry weight in Mn-supplemented brain homogenate.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000042 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000042 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:27908423 |texte= Copper brain protein protection against free radical-induced neuronal death: Survival ratio in SH-SY5Y neuroblastoma cell cultures. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:27908423" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a ParkinsonFranceV1
![]() | This area was generated with Dilib version V0.6.29. | ![]() |