Inhibitory effect of platelet factor 4 on human erythroleukemic cells is dependent on cell surface heparan sulfate
Identifieur interne : 001C42 ( Istex/Curation ); précédent : 001C41; suivant : 001C43Inhibitory effect of platelet factor 4 on human erythroleukemic cells is dependent on cell surface heparan sulfate
Auteurs : Anne-Marie Maurer [France] ; Zhong Chao Han [France] ; Didier Dhermy [France] ; Jean Briere [France]Source :
- The Journal of Laboratory and Clinical Medicine [ 0022-2143 ] ; 1996.
Abstract
We have previously reported that platelet factor 4 (PF4) inhibits human erythroleukemic (HEL) cell growth in a dose-dependent fashion in vitro and that PF4 binds to HEL cells in a specific, saturable, and concentration-dependent manner. In this article we demonstrate that the binding of PF4 on HEL cells and its inhibitiroy effect on HEL cell growth were mediated by heparan sulfate. We found that binding of iodine 125-labeled PF4 to HEL cells was inhibited by heparin, heparan sulfate, and dermatan sulfate and to a smaller extent by chondroitin sulfate. Ninety percent of 125I-labeled PF4 bound to HEL cells was released by cells after exposure to heparin and heparan sulfate. Treatment of cells with heparitinase and heparinase induced a decrease in the binding of 125I-labeled PF4 to cells. Binding of 125I-labeled PF4 was partially inhibited by the presence of increasing concentrations of protamine sulfate and basic fibroblast growth factor. To test whether PF4 bound to cell surface proteoglycans, proteoglycan synthesis was inhibited by using 4-methylumbelliferyl-β-D-xyloside. The binding of 125I-labeled PF4 on treated cells was decreased, and xyloside treatment of cells abrogated the biologic activity of PF4 in a plasma clot culture system. The inhibitory effect of PF4 was retained in a serum-free agar culture system, which indicates that the binding of PF4 to HEL cells induces cell growth inhibition in a direct fashion. Taken together, these findings suggest that PF4 directly acts on HEL cell growth by fixation on heparan sulfate proteoglycans on the HEL cell surface.
Url:
DOI: 10.1016/S0022-2143(96)90186-9
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001C44
Links to Exploration step
ISTEX:BE8E1B2D601E90039E3349EB6C2A299D7FD5DD5ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Inhibitory effect of platelet factor 4 on human erythroleukemic cells is dependent on cell surface heparan sulfate</title>
<author><name sortKey="Maurer, Anne Marie" sort="Maurer, Anne Marie" uniqKey="Maurer A" first="Anne-Marie" last="Maurer">Anne-Marie Maurer</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dhermy, Didier" sort="Dhermy, Didier" uniqKey="Dhermy D" first="Didier" last="Dhermy">Didier Dhermy</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Briere, Jean" sort="Briere, Jean" uniqKey="Briere J" first="Jean" last="Briere">Jean Briere</name>
<affiliation wicri:level="1"><mods:affiliation>Reprint requests: Professor Jean Brière, Service d'hématologie clinique, Hôpital Beaujon, 100, Boulevard du Général Leclerc, 92118 Clichy Cédex, France.</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Reprint requests: Professor Jean Brière, Service d'hématologie clinique, Hôpital Beaujon, 100, Boulevard du Général Leclerc, 92118 Clichy Cédex</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:BE8E1B2D601E90039E3349EB6C2A299D7FD5DD5A</idno>
<date when="1996" year="1996">1996</date>
<idno type="doi">10.1016/S0022-2143(96)90186-9</idno>
<idno type="url">https://api.istex.fr/document/BE8E1B2D601E90039E3349EB6C2A299D7FD5DD5A/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001C44</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001C44</idno>
<idno type="wicri:Area/Istex/Curation">001C42</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Inhibitory effect of platelet factor 4 on human erythroleukemic cells is dependent on cell surface heparan sulfate</title>
<author><name sortKey="Maurer, Anne Marie" sort="Maurer, Anne Marie" uniqKey="Maurer A" first="Anne-Marie" last="Maurer">Anne-Marie Maurer</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Han, Zhong Chao" sort="Han, Zhong Chao" uniqKey="Han Z" first="Zhong Chao" last="Han">Zhong Chao Han</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dhermy, Didier" sort="Dhermy, Didier" uniqKey="Dhermy D" first="Didier" last="Dhermy">Didier Dhermy</name>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Briere, Jean" sort="Briere, Jean" uniqKey="Briere J" first="Jean" last="Briere">Jean Briere</name>
<affiliation wicri:level="1"><mods:affiliation>Reprint requests: Professor Jean Brière, Service d'hématologie clinique, Hôpital Beaujon, 100, Boulevard du Général Leclerc, 92118 Clichy Cédex, France.</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Reprint requests: Professor Jean Brière, Service d'hématologie clinique, Hôpital Beaujon, 100, Boulevard du Général Leclerc, 92118 Clichy Cédex</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>INSERM U 409, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>INSERM U 409, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Service d'hématologie clinique, Hôpital Beaujon, Clichy, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Service d'hématologie clinique, Hôpital Beaujon, Clichy</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">The Journal of Laboratory and Clinical Medicine</title>
<title level="j" type="abbrev">YMLC</title>
<idno type="ISSN">0022-2143</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1996">1996</date>
<biblScope unit="volume">127</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="382">382</biblScope>
<biblScope unit="page" to="390">390</biblScope>
</imprint>
<idno type="ISSN">0022-2143</idno>
</series>
<idno type="istex">BE8E1B2D601E90039E3349EB6C2A299D7FD5DD5A</idno>
<idno type="DOI">10.1016/S0022-2143(96)90186-9</idno>
<idno type="PII">S0022-2143(96)90186-9</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-2143</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have previously reported that platelet factor 4 (PF4) inhibits human erythroleukemic (HEL) cell growth in a dose-dependent fashion in vitro and that PF4 binds to HEL cells in a specific, saturable, and concentration-dependent manner. In this article we demonstrate that the binding of PF4 on HEL cells and its inhibitiroy effect on HEL cell growth were mediated by heparan sulfate. We found that binding of iodine 125-labeled PF4 to HEL cells was inhibited by heparin, heparan sulfate, and dermatan sulfate and to a smaller extent by chondroitin sulfate. Ninety percent of 125I-labeled PF4 bound to HEL cells was released by cells after exposure to heparin and heparan sulfate. Treatment of cells with heparitinase and heparinase induced a decrease in the binding of 125I-labeled PF4 to cells. Binding of 125I-labeled PF4 was partially inhibited by the presence of increasing concentrations of protamine sulfate and basic fibroblast growth factor. To test whether PF4 bound to cell surface proteoglycans, proteoglycan synthesis was inhibited by using 4-methylumbelliferyl-β-D-xyloside. The binding of 125I-labeled PF4 on treated cells was decreased, and xyloside treatment of cells abrogated the biologic activity of PF4 in a plasma clot culture system. The inhibitory effect of PF4 was retained in a serum-free agar culture system, which indicates that the binding of PF4 to HEL cells induces cell growth inhibition in a direct fashion. Taken together, these findings suggest that PF4 directly acts on HEL cell growth by fixation on heparan sulfate proteoglycans on the HEL cell surface.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonFranceV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001C42 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001C42 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Sante |area= ParkinsonFranceV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:BE8E1B2D601E90039E3349EB6C2A299D7FD5DD5A |texte= Inhibitory effect of platelet factor 4 on human erythroleukemic cells is dependent on cell surface heparan sulfate }}
![]() | This area was generated with Dilib version V0.6.29. | ![]() |