Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides
Identifieur interne : 001A00 ( Istex/Curation ); précédent : 001999; suivant : 001A01Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides
Auteurs : Laurence Vergnaud [France] ; Denis Mariat ; Monique Zoroastro ; Valérie LauthierSource :
- ELECTROPHORESIS [ 0173-0835 ] ; 1991.
Abstract
Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.
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DOI: 10.1002/elps.1150120207
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Denis Mariat<affiliation><mods:affiliation>Centre d'Etudes du Bouchet, Vert le Petit</mods:affiliation><wicri:noCountry code="subField">Petit</wicri:noCountry></affiliation><affiliation><mods:affiliation>Centre d'Etudes du Bouchet, Vert le Petit</mods:affiliation><wicri:noCountry code="subField">Petit</wicri:noCountry></affiliation><affiliation><mods:affiliation>Centre d'Etudes du Bouchet, Vert le Petit</mods:affiliation><wicri:noCountry code="subField">Petit</wicri:noCountry></affiliation>Le document en format XML
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The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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