Actions of amantadine at synaptic and extrasynaptic cholinergic receptors in the central nervous system of the cockroach Periplaneta americana
Identifieur interne : 000F06 ( Istex/Curation ); précédent : 000F05; suivant : 000F07Actions of amantadine at synaptic and extrasynaptic cholinergic receptors in the central nervous system of the cockroach Periplaneta americana
Auteurs : A. Artola [France] ; J. J. Callec [France] ; B. Hue [France] ; J. A. David [Royaume-Uni] ; D. B. Sattelle [Royaume-Uni]Source :
- Journal of Insect Physiology [ 0022-1910 ] ; 1984.
Abstract
The effects of amantadine were investigated on cercal afferent, giant interneurone synapses and on the cell body membrane of the fast coxal depressor motoneurone (Df), in the cockroach Periplaneta americana. Bath-applied amantadine at concentrations above 2.0 × 10−5 M significantly reduced the amplitude of unitary and compound epsps recorded by sucrose-gap methods from cercal afferent, giant interneurone synapses in the desheathed sixth abdominal ganglion. Complete block of synaptic transmission was achieved at 1.0 × 10−3 M amantadine. Synaptic blockade, which was not accompanied by changes in resting potential, was almost fully reversed by washing the ganglion in normal saline. From the dose-dependence of the synaptic blocking action, a Hill coefficient of 0.94 was estimated, indicating that there is no co-operativity in the binding of amantadine to its site of action.Bath-application of amantadine (5.0 × 10−5 M) resulted in a parallel shift to the right of the dose-response curve for the depolarizing postsynaptic actions of acetylcholine. Nevertheless, even at a concentration of 2.0 × 10−3 M, amantadine failed to protect the synaptic acetylcholine receptor/ion channel complex from the blocking action of α-bungarotoxin (5.0 × 10−7 M). In addition, the block by amantadine of the acetylcholine-induced current recorded from the cell body membrane of the fast coxal depressor motoneurone (Df), was strongly dependent on membrane potential in the range − 120mV to − 70mV. An action of amantadine at the open acetylcholine receptor/ion channel complex is proposed.
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DOI: 10.1016/0022-1910(84)90001-5
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Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ</wicri:regionArea></affiliation><affiliation><mods:affiliation>To whom correspondence should be addressed.</mods:affiliation><wicri:noCountry code="no comma">To whom correspondence should be addressed.</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:4D2F4C9543F862CCDCAC899E976ACF98B4A5ED4A</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0022-1910(84)90001-5</idno><idno type="url">https://api.istex.fr/document/4D2F4C9543F862CCDCAC899E976ACF98B4A5ED4A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000F08</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F08</idno><idno type="wicri:Area/Istex/Curation">000F06</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Actions of amantadine at synaptic and extrasynaptic cholinergic receptors in the central nervous system of the cockroach Periplaneta americana</title><author><name sortKey="Artola, A" sort="Artola, A" uniqKey="Artola A" first="A." last="Artola">A. Artola</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie Animale, U.E.R. Sciences de la Vie et de l'Environment, Université de Rennes, 35042, Rennes, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie Animale, U.E.R. Sciences de la Vie et de l'Environment, Université de Rennes, 35042, Rennes</wicri:regionArea></affiliation></author><author><name sortKey="Callec, J J" sort="Callec, J J" uniqKey="Callec J" first="J. J." last="Callec">J. J. Callec</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie Animale, U.E.R. Sciences de la Vie et de l'Environment, Université de Rennes, 35042, Rennes, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie Animale, U.E.R. Sciences de la Vie et de l'Environment, Université de Rennes, 35042, Rennes</wicri:regionArea></affiliation></author><author><name sortKey="Hue, B" sort="Hue, B" uniqKey="Hue B" first="B." last="Hue">B. Hue</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie, Faculté de Médécine, 49045, Angers, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie, Faculté de Médécine, 49045, Angers</wicri:regionArea></affiliation></author><author><name sortKey="David, J A" sort="David, J A" uniqKey="David J" first="J. A." last="David">J. A. David</name><affiliation wicri:level="1"><mods:affiliation>A.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, U.K.</mods:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>A.R.C. 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Bath-applied amantadine at concentrations above 2.0 × 10−5 M significantly reduced the amplitude of unitary and compound epsps recorded by sucrose-gap methods from cercal afferent, giant interneurone synapses in the desheathed sixth abdominal ganglion. Complete block of synaptic transmission was achieved at 1.0 × 10−3 M amantadine. Synaptic blockade, which was not accompanied by changes in resting potential, was almost fully reversed by washing the ganglion in normal saline. From the dose-dependence of the synaptic blocking action, a Hill coefficient of 0.94 was estimated, indicating that there is no co-operativity in the binding of amantadine to its site of action.Bath-application of amantadine (5.0 × 10−5 M) resulted in a parallel shift to the right of the dose-response curve for the depolarizing postsynaptic actions of acetylcholine. Nevertheless, even at a concentration of 2.0 × 10−3 M, amantadine failed to protect the synaptic acetylcholine receptor/ion channel complex from the blocking action of α-bungarotoxin (5.0 × 10−7 M). In addition, the block by amantadine of the acetylcholine-induced current recorded from the cell body membrane of the fast coxal depressor motoneurone (Df), was strongly dependent on membrane potential in the range − 120mV to − 70mV. An action of amantadine at the open acetylcholine receptor/ion channel complex is proposed.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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