Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides
Identifieur interne : 001A02 ( Istex/Corpus ); précédent : 001A01; suivant : 001A03Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides
Auteurs : Laurence Vergnaud ; Denis Mariat ; Monique Zoroastro ; Valérie LauthierSource :
- ELECTROPHORESIS [ 0173-0835 ] ; 1991.
Abstract
Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.
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DOI: 10.1002/elps.1150120207
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The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Dr. Vergnaud</name><affiliations><json:string>Centre d'Etudes du Bouchet, Vert le Petit</json:string><json:string>Laboratoire de Génétipue Mléculaire, B. 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The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</abstract><qualityIndicators><score>7.615</score><pdfVersion>1.3</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1333</abstractCharCount><pdfWordCount>4999</pdfWordCount><pdfCharCount>29250</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>218</abstractWordCount></qualityIndicators><title>Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title><refBibs><json:item><host><volume>98</volume><pages><last>517</last><first>503</first></pages><author></author><title>J. Mol. 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Genet.</title></host></json:item></refBibs><genre><json:string>article</json:string></genre><host><volume>12</volume><publisherId><json:string>ELPS</json:string></publisherId><pages><total>7</total><last>140</last><first>134</first></pages><issn><json:string>0173-0835</json:string></issn><issue>2‐3</issue><subject><json:item><value>Article</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1522-2683</json:string></eissn><title>ELECTROPHORESIS</title><doi><json:string>10.1002/(ISSN)1522-2683</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>chemistry, analytical</json:string><json:string>biochemical research methods</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>chemistry</json:string><json:string>analytical chemistry</json:string></scienceMetrix></categories><publicationDate>1991</publicationDate><copyrightDate>1991</copyrightDate><doi><json:string>10.1002/elps.1150120207</json:string></doi><id>6982054B80013C4BBD7D6D6F281E6BCA097C0BC7</id><score>0.101374924</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/6982054B80013C4BBD7D6D6F281E6BCA097C0BC7/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/6982054B80013C4BBD7D6D6F281E6BCA097C0BC7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/6982054B80013C4BBD7D6D6F281E6BCA097C0BC7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 1991 VCH Verlagsgesellschaft mbH</p></availability><date>1991</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title><author xml:id="author-1"><persName><surname>Vergnaud</surname></persName><roleName type="degree">Dr.</roleName><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation><affiliation>Laboratoire de Génétipue Mléculaire, B. P. 3, Centre d'Etudes du Bouchet, F‐91710 Vertle Petit, France===</affiliation></author><author xml:id="author-2"><persName><forename type="first">Denis</forename><surname>Mariat</surname></persName><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation></author><author xml:id="author-3"><persName><forename type="first">Monique</forename><surname>Zoroastro</surname></persName><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation></author><author xml:id="author-4"><persName><forename type="first">Valérie</forename><surname>Lauthier</surname></persName><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation></author></analytic><monogr><title level="j">ELECTROPHORESIS</title><title level="j" type="abbrev">ELECTROPHORESIS</title><idno type="pISSN">0173-0835</idno><idno type="eISSN">1522-2683</idno><idno type="DOI">10.1002/(ISSN)1522-2683</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1991"></date><biblScope unit="volume">12</biblScope><biblScope unit="issue">2‐3</biblScope><biblScope unit="page" from="134">134</biblScope><biblScope unit="page" to="140">140</biblScope></imprint></monogr><idno type="istex">6982054B80013C4BBD7D6D6F281E6BCA097C0BC7</idno><idno type="DOI">10.1002/elps.1150120207</idno><idno type="ArticleID">ELPS1150120207</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990-07-26">Received</change><change when="1991">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6982054B80013C4BBD7D6D6F281E6BCA097C0BC7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1522-2683</doi><issn type="print">0173-0835</issn><issn type="electronic">1522-2683</issn><idGroup><id type="product" value="ELPS"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="ELECTROPHORESIS">ELECTROPHORESIS</title><title type="short">ELECTROPHORESIS</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="yes">10.1002/elps.v12:2/3</doi><numberingGroup><numbering type="journalVolume" number="12">12</numbering><numbering type="includedIssue">2</numbering><numbering type="includedIssue">3</numbering><numbering type="journalIssue">2‐3</numbering></numberingGroup><coverDate startDate="1991">1991</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="7" status="forIssue"><doi origin="wiley" registered="yes">10.1002/elps.1150120207</doi><idGroup><id type="unit" value="ELPS1150120207"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Articles</title></titleGroup><copyright ownership="publisher">Copyright © 1991 VCH Verlagsgesellschaft mbH</copyright><eventGroup><event type="manuscriptReceived" date="1990-07-26"></event><event type="firstOnline" date="2005-04-13"></event><event type="publishedOnlineFinalForm" date="2005-04-13"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-05"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-12"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">134</numbering><numbering type="pageLast">140</numbering></numberingGroup><correspondenceTo>Laboratoire de Génétipue Mléculaire, B. P. 3, Centre d'Etudes du Bouchet, F‐91710 Vertle Petit, France===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ELPS.ELPS1150120207.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="63"></count></countGroup><titleGroup><title type="main" xml:lang="en">Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title><title type="short" xml:lang="en">Development of synthetic DNA probes detecting new polymorphic loci</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><honorifics>Dr.</honorifics><givenNames>Gilles</givenNames><familyName>Vergnaud</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Denis</givenNames><familyName>Mariat</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Monique</givenNames><familyName>Zoroastro</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Valérie</givenNames><familyName>Lauthier</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>Centre d'Etudes du Bouchet, Vert le Petit</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Development of synthetic DNA probes detecting new polymorphic loci</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Detection of single and multiple polymorphic loci by synthetic tandem repeats of short oligonucleotides</title></titleInfo><name type="personal"><namePart type="termsOfAddress">Dr.</namePart><namePart type="family">Vergnaud</namePart><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation><affiliation>Laboratoire de Génétipue Mléculaire, B. P. 3, Centre d'Etudes du Bouchet, F‐91710 Vertle Petit, France===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Denis</namePart><namePart type="family">Mariat</namePart><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Monique</namePart><namePart type="family">Zoroastro</namePart><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Valérie</namePart><namePart type="family">Lauthier</namePart><affiliation>Centre d'Etudes du Bouchet, Vert le Petit</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">1991</dateIssued><dateCaptured encoding="w3cdtf">1990-07-26</dateCaptured><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">1</extent><extent unit="references">63</extent></physicalDescription><abstract lang="en">Loci containing tandem repeats of short sequences are sometimes associated with a high level of polymorphism due to variations in the number of repeats. The different variants can be easily characterized by Southern blotting when the repeats span a range from a few hundred bases to a few kilobases, and probes derived from such tandem repeats constitute convenient genetic markers. These structures, usually called minisatellites, are best documented in the human genome, where their number has been estimated to be at least 1500. However, their role and mode of evolution are poorly understood. We are developing tools to evaluate the number of such redunddant sequences in a genome and to gain access to new polymorphic loci. Our strategy is based on the use of polymers of oligonucleotides as DNA probes for hybridization on Southern blots. In a previous report, we made polymers with random units of 14 bp and showed that they detect multiple polymorphic loci on human genomic DNA. At present, we are testing the effect of an increase in the complexity of the polymer, as obtained by the use of a longer random unit, and the effect of slight sequence modifications to a particular tandem repeat sequence. In addition, some of these synthetic probes can detect a single polymorphic locus and directly provide new genetic markers.</abstract><relatedItem type="host"><titleInfo><title>ELECTROPHORESIS</title></titleInfo><titleInfo type="abbreviated"><title>ELECTROPHORESIS</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0173-0835</identifier><identifier type="eISSN">1522-2683</identifier><identifier type="DOI">10.1002/(ISSN)1522-2683</identifier><identifier type="PublisherID">ELPS</identifier><part><date>1991</date><detail type="volume"><caption>vol.</caption><number>12</number></detail><detail type="issue"><caption>no.</caption><number>2‐3</number></detail><extent unit="pages"><start>134</start><end>140</end><total>7</total></extent></part></relatedItem><identifier type="istex">6982054B80013C4BBD7D6D6F281E6BCA097C0BC7</identifier><identifier type="DOI">10.1002/elps.1150120207</identifier><identifier type="ArticleID">ELPS1150120207</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1991 VCH Verlagsgesellschaft mbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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