Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state
Identifieur interne : 001745 ( Istex/Corpus ); précédent : 001744; suivant : 001746Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state
Auteurs : Sylviane Bonnefoix ; Marie-France Sotto ; Remy Gressin ; Isabelle Martin ; Frederic Garban ; Dominique Leroux ; Jean-Charles Renversez ; Jean-Jacques SottoSource :
- European Journal of Haematology [ 0902-4441 ] ; 1998-08.
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- KwdEn :
Abstract
Abstract: Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10−6 mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.
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DOI: 10.1111/j.1600-0609.1998.tb01066.x
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ISTEX:D4CAAAA34F7BD131C363F695A8E64D2249912D6BLe document en format XML
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wicri:corpus="ISTEX">001745</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title><author><name sortKey="Bonnefoix, Sylviane" sort="Bonnefoix, Sylviane" uniqKey="Bonnefoix S" first="Sylviane" last="Bonnefoix">Sylviane Bonnefoix</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Albert Bonniot Institute, GRL, Rond‐Point de la Chantourne, 38706 La Tranche, France Fax: 33–4–76–54–94–54</mods:affiliation></affiliation></author><author><name sortKey="Sotto, Marie Rance" sort="Sotto, Marie Rance" uniqKey="Sotto M" first="Marie-France" last="Sotto">Marie-France Sotto</name><affiliation><mods:affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation></author><author><name sortKey="Gressin, Remy" sort="Gressin, Remy" uniqKey="Gressin R" first="Remy" last="Gressin">Remy Gressin</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation></author><author><name sortKey="Martin, Isabelle" sort="Martin, Isabelle" uniqKey="Martin I" first="Isabelle" last="Martin">Isabelle Martin</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation></author><author><name sortKey="Garban, Frederic" sort="Garban, Frederic" uniqKey="Garban F" first="Frederic" last="Garban">Frederic Garban</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation></author><author><name sortKey="Leroux, Dominique" sort="Leroux, Dominique" uniqKey="Leroux D" first="Dominique" last="Leroux">Dominique Leroux</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation></author><author><name sortKey="Renversez, Jean Harles" sort="Renversez, Jean Harles" uniqKey="Renversez J" first="Jean-Charles" last="Renversez">Jean-Charles Renversez</name><affiliation><mods:affiliation>Laboratory of Biochemistry, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation></author><author><name sortKey="Sotto, Jean Acques" sort="Sotto, Jean Acques" uniqKey="Sotto J" first="Jean-Jacques" last="Sotto">Jean-Jacques Sotto</name><affiliation><mods:affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">European Journal of Haematology</title><idno type="ISSN">0902-4441</idno><idno type="eISSN">1600-0609</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1998-08">1998-08</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="84">84</biblScope><biblScope unit="page" to="92">92</biblScope></imprint><idno type="ISSN">0902-4441</idno></series><idno type="istex">D4CAAAA34F7BD131C363F695A8E64D2249912D6B</idno><idno type="DOI">10.1111/j.1600-0609.1998.tb01066.x</idno><idno type="ArticleID">EJH1066</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0902-4441</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>differentiation</term><term>interferon</term><term>lymphoma</term><term>retinoids</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Abstract: Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10−6 mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Dr. Bonnefoix</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string><json:string>Department of Haematology, CHU Michallon, 38706 Grenoble, France</json:string><json:string>Albert Bonniot Institute, GRL, Rond‐Point de la Chantourne, 38706 La Tranche, France Fax: 33–4–76–54–94–54</json:string></affiliations></json:item><json:item><name>Marie‐France Sotto</name><affiliations><json:string>Department of Haematology, CHU Michallon, 38706 Grenoble, France</json:string></affiliations></json:item><json:item><name>Remy Gressin</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string><json:string>Department of Haematology, CHU Michallon, 38706 Grenoble, France</json:string></affiliations></json:item><json:item><name>Isabelle Martin</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string></affiliations></json:item><json:item><name>Frederic Garban</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string><json:string>Department of Haematology, CHU Michallon, 38706 Grenoble, France</json:string></affiliations></json:item><json:item><name>Dominique Leroux</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string></affiliations></json:item><json:item><name>Jean‐Charles Renversez</name><affiliations><json:string>Laboratory of Biochemistry, CHU Michallon, 38706 Grenoble, France</json:string></affiliations></json:item><json:item><name>Jean‐Jacques Sotto</name><affiliations><json:string>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</json:string><json:string>Department of Haematology, CHU Michallon, 38706 Grenoble, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>lymphoma</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>interferon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>retinoids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>differentiation</value></json:item></subject><articleId><json:string>EJH1066</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Abstract: Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10−6 mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.</abstract><qualityIndicators><score>7.262</score><pdfVersion>1.3</pdfVersion><pdfPageSize>625.559 x 858.599 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1765</abstractCharCount><pdfWordCount>4298</pdfWordCount><pdfCharCount>29826</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>247</abstractWordCount></qualityIndicators><title>Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title><refBibs><json:item><author><json:item><name>JU. 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Shiota</name></json:item><json:item><name>M. Takanashi</name></json:item></author><host><volume>72</volume><pages><last>2694</last><first>2686</first></pages><author></author><title>Cancer</title></host><title>Engraftment of human non‐Hodgkin lymphomas in mice with severe combined immunodeficiency</title></json:item></refBibs><genre><json:string>article</json:string></genre><host><volume>61</volume><publisherId><json:string>EJH</json:string></publisherId><pages><total>9</total><last>92</last><first>84</first></pages><issn><json:string>0902-4441</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1600-0609</json:string></eissn><title>European Journal of Haematology</title><doi><json:string>10.1111/(ISSN)1600-0609</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>hematology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>immunology</json:string></scienceMetrix></categories><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1111/j.1600-0609.1998.tb01066.x</json:string></doi><id>D4CAAAA34F7BD131C363F695A8E64D2249912D6B</id><score>0.17053391</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/D4CAAAA34F7BD131C363F695A8E64D2249912D6B/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/D4CAAAA34F7BD131C363F695A8E64D2249912D6B/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/D4CAAAA34F7BD131C363F695A8E64D2249912D6B/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© Munksgaard 1998</p></availability><date>1998</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title><author xml:id="author-1"><persName><surname>Bonnefoix</surname></persName><roleName type="degree">Dr.</roleName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><affiliation>Albert Bonniot Institute, GRL, Rond‐Point de la Chantourne, 38706 La Tranche, France Fax: 33–4–76–54–94–54</affiliation></author><author xml:id="author-2"><persName><forename type="first">Marie‐France</forename><surname>Sotto</surname></persName><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Remy</forename><surname>Gressin</surname></persName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Isabelle</forename><surname>Martin</surname></persName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">Frederic</forename><surname>Garban</surname></persName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">Dominique</forename><surname>Leroux</surname></persName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">Jean‐Charles</forename><surname>Renversez</surname></persName><affiliation>Laboratory of Biochemistry, CHU Michallon, 38706 Grenoble, France</affiliation></author><author xml:id="author-8"><persName><forename type="first">Jean‐Jacques</forename><surname>Sotto</surname></persName><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation></author></analytic><monogr><title level="j">European Journal of Haematology</title><idno type="pISSN">0902-4441</idno><idno type="eISSN">1600-0609</idno><idno type="DOI">10.1111/(ISSN)1600-0609</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1998-08"></date><biblScope unit="volume">61</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="84">84</biblScope><biblScope unit="page" to="92">92</biblScope></imprint></monogr><idno type="istex">D4CAAAA34F7BD131C363F695A8E64D2249912D6B</idno><idno type="DOI">10.1111/j.1600-0609.1998.tb01066.x</idno><idno type="ArticleID">EJH1066</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Abstract: Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10−6 mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>lymphoma</term></item><item><term>interferon</term></item><item><term>retinoids</term></item><item><term>differentiation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/D4CAAAA34F7BD131C363F695A8E64D2249912D6B/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: 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startDate="1998-08">August 1998</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="3" status="forIssue"><doi origin="wiley">10.1111/j.1600-0609.1998.tb01066.x</doi><idGroup><id type="unit" value="EJH1066"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><copyright>© Munksgaard 1998</copyright><eventGroup><event type="firstOnline" date="2009-04-24"></event><event type="publishedOnlineFinalForm" date="2009-04-24"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-21"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-21"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering 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corresponding="yes"><personName><honorifics>Dr.</honorifics><givenNames>Thierry</givenNames><familyName>Bonnefoix</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Marie‐France</givenNames><familyName>Sotto</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1 #a2"><personName><givenNames>Remy</givenNames><familyName>Gressin</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>Isabelle</givenNames><familyName>Martin</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1 #a2"><personName><givenNames>Frederic</givenNames><familyName>Garban</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>Dominique</givenNames><familyName>Leroux</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a3"><personName><givenNames>Jean‐Charles</givenNames><familyName>Renversez</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a1 #a2"><personName><givenNames>Jean‐Jacques</givenNames><familyName>Sotto</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Laboratory of Biochemistry, CHU Michallon, 38706 Grenoble, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">lymphoma</keyword><keyword xml:id="k2">interferon</keyword><keyword xml:id="k3">retinoids</keyword><keyword xml:id="k4">differentiation</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p><b>Abstract: </b> Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10<sup>−6</sup> mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Phenotypic, morphologic changes and Ig secretion induced on B‐NHL cells in vitro by interferon alpha and all‐trans‐retinoic acid: possible progression toward a more differentiated state</title></titleInfo><name type="personal"><namePart type="termsOfAddress">Dr.</namePart><namePart type="family">Bonnefoix</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><affiliation>Albert Bonniot Institute, GRL, Rond‐Point de la Chantourne, 38706 La Tranche, France Fax: 33–4–76–54–94–54</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marie‐France</namePart><namePart type="family">Sotto</namePart><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Remy</namePart><namePart type="family">Gressin</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Martin</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frederic</namePart><namePart type="family">Garban</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dominique</namePart><namePart type="family">Leroux</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean‐Charles</namePart><namePart type="family">Renversez</namePart><affiliation>Laboratory of Biochemistry, CHU Michallon, 38706 Grenoble, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean‐Jacques</namePart><namePart type="family">Sotto</namePart><affiliation>Lymphoma Research Group, Albert Bonniot Research Institute, Rond‐Point de la Chantourne, 38706 La Tranche, France</affiliation><affiliation>Department of Haematology, CHU Michallon, 38706 Grenoble, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1998-08</dateIssued><edition>Accepted for publication 2 February 1998</edition><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">20</extent></physicalDescription><abstract>Abstract: Twenty‐five B‐cell‐nonHodgkin's lymphomas (B‐NHL): 6 lymphocytic, 2 centrocytic, 13 follicular, centrocytic/centroblastic, 2 lymphoplasmocytoid and 2 centroblastic were tested for their ability to acquire features of mature plasma cells under the effect of interferon alpha (final concentration, 600 Ul/ml), all‐trans‐retinoic‐acid (ATRA) (final concentration, 10−6 mol/1) and the association of both. B‐NHL cells were negatively purified (>99%) by an immunomagnetic method, cultured for 7 d with or without interferon and ATRA, then stained with anti‐CD 19, CD20, surface Ig, DR, CD38 and with anti‐CD138 (syndecan‐1) antibody‐recognizing plasma cells. Ig production was estimated in culture supernatants by an ELISA method and changes in cell morphology were investigated on May–Grünwald–Giemsa‐stained cytospin preparations. In all cases interferon and ATRA, alone or in association, were able to induce changes in the immunophenotypic profile, associated or not with morphologic changes and induction of Ig secretion. All changes were greatly variable from one to the other B‐NHL sample and no relationship could be found between a particular pattern of change and the histological subtype. Interferon alpha was more potent than ATRA in inducing changes. In favour of a differentiation process, we observed a concomitant decrease of DR expression and increase of CD38 expression in 8 cases with interferon alpha, and in 4 cases with ATRA. Although interferon‐ or ATRA‐treated cells did not display cytologic, functional features and changes of the immunophenotypic profile fully compatible with those of terminally differentiated cells, these results suggest a possible transition toward more differentiated elements, especially with interferon alpha.</abstract><subject lang="en"><genre>keywords</genre><topic>lymphoma</topic><topic>interferon</topic><topic>retinoids</topic><topic>differentiation</topic></subject><relatedItem type="host"><titleInfo><title>European Journal of Haematology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0902-4441</identifier><identifier type="eISSN">1600-0609</identifier><identifier type="DOI">10.1111/(ISSN)1600-0609</identifier><identifier type="PublisherID">EJH</identifier><part><date>1998</date><detail type="volume"><caption>vol.</caption><number>61</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>84</start><end>92</end><total>9</total></extent></part></relatedItem><identifier type="istex">D4CAAAA34F7BD131C363F695A8E64D2249912D6B</identifier><identifier type="DOI">10.1111/j.1600-0609.1998.tb01066.x</identifier><identifier type="ArticleID">EJH1066</identifier><accessCondition type="use and reproduction" contentType="copyright">© Munksgaard 1998</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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