In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis
Identifieur interne : 001621 ( Istex/Corpus ); précédent : 001620; suivant : 001622In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis
Auteurs : M. Barral-Netto ; R. L. Von Sohsten ; M. Teixeira ; W. L. Conrado Dos Santos ; M. L. Pompeu ; R. A. Moreira ; J. T. A. Oliveira ; B. S. Cavada ; E. Falcoff ; A. BarralSource :
- Acta Tropica [ 0001-706X ] ; 1996.
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Abstract
In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.
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DOI: 10.1016/0001-706X(95)00120-4
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ISTEX:32EF19B1D2BDC6AD12A7CA2BDECB7F2D4794D9CDLe document en format XML
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Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Teixeira, M" sort="Teixeira, M" uniqKey="Teixeira M" first="M." last="Teixeira">M. Teixeira</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Conrado Dos Santos, W L" sort="Conrado Dos Santos, W L" uniqKey="Conrado Dos Santos W" first="W. L." last="Conrado Dos Santos">W. L. Conrado Dos Santos</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Pompeu, M L" sort="Pompeu, M L" uniqKey="Pompeu M" first="M. L." last="Pompeu">M. L. 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Barral-Netto</name><affiliation><mods:affiliation>E-mail: barral@ufba.br</mods:affiliation></affiliation><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Von Sohsten, R L" sort="Von Sohsten, R L" uniqKey="Von Sohsten R" first="R. L." last="Von Sohsten">R. L. Von Sohsten</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Teixeira, M" sort="Teixeira, M" uniqKey="Teixeira M" first="M." last="Teixeira">M. Teixeira</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Conrado Dos Santos, W L" sort="Conrado Dos Santos, W L" uniqKey="Conrado Dos Santos W" first="W. L." last="Conrado Dos Santos">W. L. Conrado Dos Santos</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Pompeu, M L" sort="Pompeu, M L" uniqKey="Pompeu M" first="M. L." last="Pompeu">M. L. Pompeu</name><affiliation><mods:affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Moreira, R A" sort="Moreira, R A" uniqKey="Moreira R" first="R. A." last="Moreira">R. A. Moreira</name><affiliation><mods:affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Oliveira, J T A" sort="Oliveira, J T A" uniqKey="Oliveira J" first="J. T. A." last="Oliveira">J. T. A. Oliveira</name><affiliation><mods:affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Cavada, B S" sort="Cavada, B S" uniqKey="Cavada B" first="B. S." last="Cavada">B. S. Cavada</name><affiliation><mods:affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</mods:affiliation></affiliation></author><author><name sortKey="Falcoff, E" sort="Falcoff, E" uniqKey="Falcoff E" first="E." last="Falcoff">E. Falcoff</name><affiliation><mods:affiliation>Institut Curie, Unité INSERM 196, Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Barral, A" sort="Barral, A" uniqKey="Barral A" first="A." last="Barral">A. Barral</name><affiliation><mods:affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta Tropica</title><title level="j" type="abbrev">ACTROP</title><idno type="ISSN">0001-706X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="237">237</biblScope><biblScope unit="page" to="250">250</biblScope></imprint><idno type="ISSN">0001-706X</idno></series><idno type="istex">32EF19B1D2BDC6AD12A7CA2BDECB7F2D4794D9CD</idno><idno type="DOI">10.1016/0001-706X(95)00120-4</idno><idno type="PII">0001-706X(95)00120-4</idno><idno type="ArticleID">95001204</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0001-706X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Canavalia brasiliensis</term><term>Experimental leishmaniasis</term><term>Interferon-γ</term><term>Leishmania</term><term>Leishmania amazonensis</term><term>Leishmaniasis</term><term>Tegumentary leishmaniasis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>M. Barral-Netto</name><affiliations><json:string>E-mail: barral@ufba.br</json:string><json:string>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</json:string></affiliations></json:item><json:item><name>R.L. Von Sohsten</name><affiliations><json:string>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</json:string></affiliations></json:item><json:item><name>M. Teixeira</name><affiliations><json:string>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</json:string></affiliations></json:item><json:item><name>W.L. Conrado dos Santos</name><affiliations><json:string>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</json:string></affiliations></json:item><json:item><name>M.L. Pompeu</name><affiliations><json:string>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</json:string></affiliations></json:item><json:item><name>R.A. Moreira</name><affiliations><json:string>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</json:string></affiliations></json:item><json:item><name>J.T.A. Oliveira</name><affiliations><json:string>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</json:string></affiliations></json:item><json:item><name>B.S. Cavada</name><affiliations><json:string>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</json:string></affiliations></json:item><json:item><name>E. Falcoff</name><affiliations><json:string>Institut Curie, Unité INSERM 196, Paris, France</json:string></affiliations></json:item><json:item><name>A. Barral</name><affiliations><json:string>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Leishmania amazonensis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Leishmaniasis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Leishmania</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Tegumentary leishmaniasis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Experimental leishmaniasis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Canavalia brasiliensis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Interferon-γ</value></json:item></subject><articleId><json:string>95001204</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.</abstract><qualityIndicators><score>7.088</score><pdfVersion>1.2</pdfVersion><pdfPageSize>461 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>7</keywordCount><abstractCharCount>1217</abstractCharCount><pdfWordCount>5284</pdfWordCount><pdfCharCount>32576</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>174</abstractWordCount></qualityIndicators><title>In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis</title><pii><json:string>0001-706X(95)00120-4</json:string></pii><refBibs><json:item><author><json:item><name>Z.A. Andrade</name></json:item><json:item><name>S.G. Reed</name></json:item><json:item><name>S.B. Roters</name></json:item><json:item><name>M. 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R. João das Botas, s/n. 40.140 Salvador, Bahia, Brazil. Phone (55-71) 237-7353; FAX (55-71) 245-7110.</p></note><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation></author><author xml:id="author-2"><persName><forename type="first">R.L.</forename><surname>Von Sohsten</surname></persName><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation></author><author xml:id="author-3"><persName><forename type="first">M.</forename><surname>Teixeira</surname></persName><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation></author><author xml:id="author-4"><persName><forename type="first">W.L.</forename><surname>Conrado dos Santos</surname></persName><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation></author><author xml:id="author-5"><persName><forename type="first">M.L.</forename><surname>Pompeu</surname></persName><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation></author><author xml:id="author-6"><persName><forename type="first">R.A.</forename><surname>Moreira</surname></persName><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation></author><author xml:id="author-7"><persName><forename type="first">J.T.A.</forename><surname>Oliveira</surname></persName><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation></author><author xml:id="author-8"><persName><forename type="first">B.S.</forename><surname>Cavada</surname></persName><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation></author><author xml:id="author-9"><persName><forename type="first">E.</forename><surname>Falcoff</surname></persName><affiliation>Institut Curie, Unité INSERM 196, Paris, France</affiliation></author><author xml:id="author-10"><persName><forename type="first">A.</forename><surname>Barral</surname></persName><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation></author></analytic><monogr><title level="j">Acta Tropica</title><title level="j" type="abbrev">ACTROP</title><idno type="pISSN">0001-706X</idno><idno type="PII">S0001-706X(00)X0004-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">60</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="237">237</biblScope><biblScope unit="page" to="250">250</biblScope></imprint></monogr><idno type="istex">32EF19B1D2BDC6AD12A7CA2BDECB7F2D4794D9CD</idno><idno type="DOI">10.1016/0001-706X(95)00120-4</idno><idno type="PII">0001-706X(95)00120-4</idno><idno type="ArticleID">95001204</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Leishmania amazonensis</term></item><item><term>Leishmaniasis</term></item><item><term>Leishmania</term></item><item><term>Tegumentary leishmaniasis</term></item><item><term>Experimental leishmaniasis</term></item><item><term>Canavalia brasiliensis</term></item><item><term>Interferon-γ</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/32EF19B1D2BDC6AD12A7CA2BDECB7F2D4794D9CD/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>ACTROP</jid><aid>95001204</aid><ce:pii>0001-706X(95)00120-4</ce:pii><ce:doi>10.1016/0001-706X(95)00120-4</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright></item-info><head><ce:title>In vivo protective effect of the lectin from <ce:italic>Canavalia brasiliensis</ce:italic> on BALB/c mice infected by <ce:italic>Leishmania amazonensis</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Barral-Netto</ce:surname><ce:cross-ref refid="COR1"><ce:sup loc="post">∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref><ce:e-address type="email">barral@ufba.br</ce:e-address></ce:author><ce:author><ce:given-name>R.L.</ce:given-name><ce:surname>Von Sohsten</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Teixeira</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>W.L.</ce:given-name><ce:surname>Conrado dos Santos</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.L.</ce:given-name><ce:surname>Pompeu</ce:surname><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.A.</ce:given-name><ce:surname>Moreira</ce:surname><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.T.A.</ce:given-name><ce:surname>Oliveira</ce:surname><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>B.S.</ce:given-name><ce:surname>Cavada</ce:surname><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Falcoff</ce:surname><ce:cross-ref refid="AFF4"><ce:sup loc="post">d</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Barral</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>LIMI, Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Salvador, Bahia, Brazil</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Institut Curie, Unité INSERM 196, Paris, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence address: Dr. Manoel Barral-Netto, Serviço de Imunologia-HUPES-UFBA. R. João das Botas, s/n. 40.140 Salvador, Bahia, Brazil. Phone (55-71) 237-7353; FAX (55-71) 245-7110.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="9" month="8" year="1995"></ce:date-received><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">In vivo administration of <ce:italic>Canavalia brasiliensis</ce:italic> lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by <ce:italic>Leishmania amazonensis</ce:italic>. At the doses used <ce:italic>C. brasiliensis</ce:italic> lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on <ce:italic>L. amazonensis</ce:italic>-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked <ce:italic>L. amazonensis</ce:italic> infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Leishmania amazonensis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Leishmaniasis</ce:text></ce:keyword><ce:keyword><ce:text>Leishmania</ce:text></ce:keyword><ce:keyword><ce:text>Tegumentary leishmaniasis</ce:text></ce:keyword><ce:keyword><ce:text>Experimental leishmaniasis</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Canavalia brasiliensis</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Interferon-γ</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>In vivo protective effect of the lectin from Canavalia brasiliensis on BALB/c mice infected by Leishmania amazonensis</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>In vivo protective effect of the lectin from</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Barral-Netto</namePart><affiliation>E-mail: barral@ufba.br</affiliation><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation><description>Correspondence address: Dr. Manoel Barral-Netto, Serviço de Imunologia-HUPES-UFBA. R. João das Botas, s/n. 40.140 Salvador, Bahia, Brazil. Phone (55-71) 237-7353; FAX (55-71) 245-7110.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.L.</namePart><namePart type="family">Von Sohsten</namePart><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Teixeira</namePart><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.L.</namePart><namePart type="family">Conrado dos Santos</namePart><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.L.</namePart><namePart type="family">Pompeu</namePart><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.A.</namePart><namePart type="family">Moreira</namePart><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.T.A.</namePart><namePart type="family">Oliveira</namePart><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.S.</namePart><namePart type="family">Cavada</namePart><affiliation>Departamentos de Patologia & Bioquimica, Universidade Federal do Ceará, Ceara, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Falcoff</namePart><affiliation>Institut Curie, Unité INSERM 196, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Barral</namePart><affiliation>Serviço de Imunologia, Hosp. Univ. Prof. Edgard Santos, Universidade Federal da Bahia, Bahia, Brazil</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">In vivo administration of Canavalia brasiliensis lectin (at the time of infection, or maintained throughout the infection) reduced the lesions of highly susceptible BALB/c mice infected by Leishmania amazonensis. At the doses used C. brasiliensis lectin (ConBr) does not interfere with penetration or fate of Leishmania in the macrophages in vitro. Since Interferon-γ (IFN-γ) is the major macrophage activating factor, and considered a critical element in the successful immune response against leishmaniasis, we explored its participation in this phenomenon. ConBr either in vivo or in vitro induced IFN-γ production in normal or in leishmania-infected BALB/c mice. However we were unable to change the course of disease by in vivo IFN-γ administration (although IFN-γ preparations were effective in inducing a leishmanicidal effect in vitro on L. amazonensis-infected peritoneal macrophages). Additionally, IFN-γ neutralization with anti-IFN-γ monoclonal antibody did not alter the protection conferred by ConBr administration. These data show that lectin administration in vivo is protective in the otherwise unchecked L. amazonensis infection of BALB/c mice, and suggest that such effect is not mediated by IFN-γ.</abstract><subject lang="en"><genre>Keywords</genre><topic>Leishmania amazonensis</topic><topic>Leishmaniasis</topic><topic>Leishmania</topic><topic>Tegumentary leishmaniasis</topic><topic>Experimental leishmaniasis</topic><topic>Canavalia brasiliensis</topic><topic>Interferon-γ</topic></subject><relatedItem type="host"><titleInfo><title>Acta Tropica</title></titleInfo><titleInfo type="abbreviated"><title>ACTROP</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199602</dateIssued></originInfo><identifier type="ISSN">0001-706X</identifier><identifier type="PII">S0001-706X(00)X0004-6</identifier><part><date>199602</date><detail type="volume"><number>60</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue pages"><start>221</start><end>303</end></extent><extent unit="pages"><start>237</start><end>250</end></extent></part></relatedItem><identifier type="istex">32EF19B1D2BDC6AD12A7CA2BDECB7F2D4794D9CD</identifier><identifier type="DOI">10.1016/0001-706X(95)00120-4</identifier><identifier type="PII">0001-706X(95)00120-4</identifier><identifier type="ArticleID">95001204</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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