Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system
Identifieur interne : 001489 ( Istex/Corpus ); précédent : 001488; suivant : 001490Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system
Auteurs : Dom Miceli ; Jacques Repérant ; Jean-Paul Rio ; Jean Désilets ; Monique MédinaSource :
- Brain Research [ 0006-8993 ] ; 2000.
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Abstract
A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.
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DOI: 10.1016/S0006-8993(00)02316-7
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last="Rio">Jean-Paul Rio</name><affiliation><mods:affiliation>E-mail: rio@infobiogen.fr</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Desilets, Jean" sort="Desilets, Jean" uniqKey="Desilets J" first="Jean" last="Désilets">Jean Désilets</name><affiliation><mods:affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</mods:affiliation></affiliation></author><author><name sortKey="Medina, Monique" sort="Medina, Monique" uniqKey="Medina M" first="Monique" last="Médina">Monique Médina</name><affiliation><mods:affiliation>Laboratoire d’Anatomie comparée, MNHN, Paris, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno 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Canada</mods:affiliation></affiliation></author><author><name sortKey="Reperant, Jacques" sort="Reperant, Jacques" uniqKey="Reperant J" first="Jacques" last="Repérant">Jacques Repérant</name><affiliation><mods:affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</mods:affiliation></affiliation></author><author><name sortKey="Rio, Jean Paul" sort="Rio, Jean Paul" uniqKey="Rio J" first="Jean-Paul" last="Rio">Jean-Paul Rio</name><affiliation><mods:affiliation>E-mail: rio@infobiogen.fr</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</mods:affiliation></affiliation></author><author><name sortKey="Desilets, Jean" sort="Desilets, Jean" uniqKey="Desilets J" first="Jean" last="Désilets">Jean Désilets</name><affiliation><mods:affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</mods:affiliation></affiliation></author><author><name sortKey="Medina, Monique" sort="Medina, Monique" uniqKey="Medina M" first="Monique" last="Médina">Monique Médina</name><affiliation><mods:affiliation>Laboratoire d’Anatomie comparée, MNHN, Paris, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">868</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="128">128</biblScope><biblScope unit="page" to="134">134</biblScope></imprint><idno type="ISSN">0006-8993</idno></series><idno type="istex">80EC10732A9863B90356AB25C6BE4CE6C3850C17</idno><idno type="DOI">10.1016/S0006-8993(00)02316-7</idno><idno type="PII">S0006-8993(00)02316-7</idno><idno type="ArticleID">28406</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Afferent synaptic terminals</term><term>Centrifugal visual system</term><term>Electron microscopy</term><term>Glutamate immunoreactivity</term><term>Isthmo-optic nucleus</term><term>Pigeon</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Dom Miceli</name><affiliations><json:string>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</json:string></affiliations></json:item><json:item><name>Jacques Repérant</name><affiliations><json:string>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</json:string></affiliations></json:item><json:item><name>Jean-Paul Rio</name><affiliations><json:string>E-mail: rio@infobiogen.fr</json:string><json:string>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</json:string></affiliations></json:item><json:item><name>Jean Désilets</name><affiliations><json:string>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</json:string></affiliations></json:item><json:item><name>Monique Médina</name><affiliations><json:string>Laboratoire d’Anatomie comparée, MNHN, Paris, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Centrifugal visual system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Isthmo-optic nucleus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Afferent synaptic terminals</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Glutamate immunoreactivity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Pigeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Electron microscopy</value></json:item></subject><articleId><json:string>28406</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.</abstract><qualityIndicators><score>6.965</score><pdfVersion>1.2</pdfVersion><pdfPageSize>598 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>1525</abstractCharCount><pdfWordCount>4337</pdfWordCount><pdfCharCount>28148</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>219</abstractWordCount></qualityIndicators><title>Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</title><pii><json:string>S0006-8993(00)02316-7</json:string></pii><refBibs><json:item><author><json:item><name>P. Angaut</name></json:item><json:item><name>J. 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II: The distribution of the nerve fibers</title></json:item></refBibs><genre><json:string>brief-communication</json:string></genre><host><volume>868</volume><pii><json:string>S0006-8993(00)X0272-7</json:string></pii><pages><last>134</last><first>128</first></pages><issn><json:string>0006-8993</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Brain Research</title><publicationDate>2000</publicationDate></host><categories><wos><json:string>science</json:string><json:string>neurosciences</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0006-8993(00)02316-7</json:string></doi><id>80EC10732A9863B90356AB25C6BE4CE6C3850C17</id><score>0.11649449</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/80EC10732A9863B90356AB25C6BE4CE6C3850C17/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/80EC10732A9863B90356AB25C6BE4CE6C3850C17/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/80EC10732A9863B90356AB25C6BE4CE6C3850C17/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Elsevier Science B.V.</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Section title: Short communication</note><note type="content">Fig. 1: Electron micrographs of glutamate-immunoreactive (A–C) and -immunonegative (D) axon terminals within the pigeon ION. (A) Large-sized P1a axon terminal with a clear axoplasm. The synaptic vesicles are oval to rounded-shaped. This establishes an asymmetrical contact (arrow) on a dendritic profile. (B) Small-sized P1b axon terminal. As for P1a type, the synaptic vesicles are rounded, but more minute. Note that the synaptic zone is longer than in P1a (arrows) and also asymmetrical. The arrowhead points to a dense-core vesicle. (C) P2b axon terminal with a clear-medium-dense axoplasm, containing numerous pleomorphic synaptic vesicles, and making a symmetrical contact (arrow) on a large dendritic profile. (D) Characteristic P2c axon terminal, containing a dense population of pleomorphic synaptic vesicles, and a long-sized synaptic zone (arrow), with a symmetrical contact on a proximal dendrite of a centrifugal neuron. Scale bars: 0.5 μm.</note><note type="content">Fig. 2: Examples of single glutamate (A–C) and double GABA-glutamate (D) immunolabeling in the pigeon ION. (A) The upper left shows a GLU-ir P1a axon terminal contacting a long spine-like profile (arrow). This micrograph also illustrates a P3 axon terminal with rounded-shaped synaptic vesicles, establishing an asymmetrical contact (arrow) upon dendrite of an ION centrifugal neuron. Note the presence of dense-core vesicles (arrowhead). (B) Another large P1a axon terminal is close to a P4 GLU-immunonegative bouton with a clear axoplasm in which the synaptic vesicles are dense. The synaptic zone is long and asymmetrical (arrow). Note the presence of a clear-cut postsynaptic differentiation. (C) The left part of the micrograph is occupied by a P5 axon terminal. The axoplasm is clear and contains a mixture of small rounded synaptic vesicles and numerous large dense-core vesicles. No glutamate immunoreactivity is present. Another P1b axon terminal lies in the upper right part of the micrograph. (D) P2a axon terminal characterized by pleomorphic synaptic vesicles which make a symmetrical synaptic contact (arrow) on a large dendritic profile of an ION centrifugal neuron. Whereas the bouton is GABA-immunoreactive, the postsynaptic dendrite is densely GLU-ir. Scale bars: 0.5 μm.</note><note type="content">Table 1: Glutamate immunogold labeling</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</title><author xml:id="author-1"><persName><forename type="first">Dom</forename><surname>Miceli</surname></persName><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation></author><author xml:id="author-2"><persName><forename type="first">Jacques</forename><surname>Repérant</surname></persName><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jean-Paul</forename><surname>Rio</surname></persName><email>rio@infobiogen.fr</email><note type="correspondence"><p>Corresponding author. Tel.: +33-11-4216-2678; fax: +33-4570-9990</p></note><affiliation>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Jean</forename><surname>Désilets</surname></persName><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation></author><author xml:id="author-5"><persName><forename type="first">Monique</forename><surname>Médina</surname></persName><affiliation>Laboratoire d’Anatomie comparée, MNHN, Paris, France</affiliation></author></analytic><monogr><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="pISSN">0006-8993</idno><idno type="PII">S0006-8993(00)X0272-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">868</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="128">128</biblScope><biblScope unit="page" to="134">134</biblScope></imprint></monogr><idno type="istex">80EC10732A9863B90356AB25C6BE4CE6C3850C17</idno><idno type="DOI">10.1016/S0006-8993(00)02316-7</idno><idno type="PII">S0006-8993(00)02316-7</idno><idno type="ArticleID">28406</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Centrifugal visual system</term></item><item><term>Isthmo-optic nucleus</term></item><item><term>Afferent synaptic terminals</term></item><item><term>Glutamate immunoreactivity</term></item><item><term>Pigeon</term></item><item><term>Electron microscopy</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/80EC10732A9863B90356AB25C6BE4CE6C3850C17/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco" xml:lang="en"><item-info><jid>BRES</jid><aid>28406</aid><ce:pii>S0006-8993(00)02316-7</ce:pii><ce:doi>10.1016/S0006-8993(00)02316-7</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science B.V.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Short communication</ce:textfn></ce:dochead><ce:title>Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</ce:title><ce:author-group><ce:author><ce:given-name>Dom</ce:given-name><ce:surname>Miceli</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jacques</ce:given-name><ce:surname>Repérant</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jean-Paul</ce:given-name><ce:surname>Rio</ce:surname><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address type="email">rio@infobiogen.fr</ce:e-address></ce:author><ce:author><ce:given-name>Jean</ce:given-name><ce:surname>Désilets</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Monique</ce:given-name><ce:surname>Médina</ce:surname><ce:cross-ref refid="AFF3"><ce:sup loc="post">c</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Laboratoire d’Anatomie comparée, MNHN, Paris, France</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +33-11-4216-2678; fax: +33-4570-9990</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="21" month="3" year="2000"></ce:date-accepted><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0025">A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Centrifugal visual system</ce:text></ce:keyword><ce:keyword><ce:text>Isthmo-optic nucleus</ce:text></ce:keyword><ce:keyword><ce:text>Afferent synaptic terminals</ce:text></ce:keyword><ce:keyword><ce:text>Glutamate immunoreactivity</ce:text></ce:keyword><ce:keyword><ce:text>Pigeon</ce:text></ce:keyword><ce:keyword><ce:text>Electron microscopy</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Quantitative immunogold evidence that glutamate is a neurotransmitter in afferent synaptic terminals within the isthmo-optic nucleus of the pigeon centrifugal visual system</title></titleInfo><name type="personal"><namePart type="given">Dom</namePart><namePart type="family">Miceli</namePart><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacques</namePart><namePart type="family">Repérant</namePart><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Paul</namePart><namePart type="family">Rio</namePart><affiliation>E-mail: rio@infobiogen.fr</affiliation><affiliation>Laboratoire de Neuromorphologie, Développement, Evolution, INSERM U 106, Hôpital de la Salpêtrière, Paris, France</affiliation><description>Corresponding author. Tel.: +33-11-4216-2678; fax: +33-4570-9990</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean</namePart><namePart type="family">Désilets</namePart><affiliation>Laboratoire de Neuropsychologie expérimentale et comparée, Université du Québec, Trois-Rivières, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Monique</namePart><namePart type="family">Médina</namePart><affiliation>Laboratoire d’Anatomie comparée, MNHN, Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A quantitative electron microscopic analysis of glutamate (GLU) immunoreactivity using the post-embedding immunogold technique was carried out within the isthmo-optic nucleus (ION) of the pigeon centrifugal visual system (CVS). Measurements were performed in each of eight different categories of axon terminals, including those that were GABA-immunoreactive (-ir), considered representing control profiles and identified using a single or double-label immunocytochemical procedure. The results demonstrated that the glutamate immunogold particle densities for both mitochondrial and vesicular pools and for total surface area of bouton profiles were significantly higher in P1a, P1b and P2b terminals and not significantly different in P4 and P5 terminals compared to those recorded in control GABA-ir terminals (P2a, P2c, P3). Moreover, the values measured in GLU-ir positive profiles were all significantly higher than in either P4 or P5 terminals. The results suggest that tectal neurons, which provide the main input to the ION cells, are either inhibitory GABA-ir possibly associated with P2c and/or P3 terminals or excitatory GLU-ir via P1a, P1b and P2b terminals. Such differential effects of tectal afferents may be the basis for the modulation of centrifugal activity and consequently of end target retinal ganglion cell responses. The data are relevant to hypotheses implicating the avian CVS in mechanisms of selective enhancement of visual attention to either novel or meaningful stimuli within the visual field.</abstract><note type="content">Section title: Short communication</note><note type="content">Fig. 1: Electron micrographs of glutamate-immunoreactive (A–C) and -immunonegative (D) axon terminals within the pigeon ION. (A) Large-sized P1a axon terminal with a clear axoplasm. The synaptic vesicles are oval to rounded-shaped. This establishes an asymmetrical contact (arrow) on a dendritic profile. (B) Small-sized P1b axon terminal. As for P1a type, the synaptic vesicles are rounded, but more minute. Note that the synaptic zone is longer than in P1a (arrows) and also asymmetrical. The arrowhead points to a dense-core vesicle. (C) P2b axon terminal with a clear-medium-dense axoplasm, containing numerous pleomorphic synaptic vesicles, and making a symmetrical contact (arrow) on a large dendritic profile. (D) Characteristic P2c axon terminal, containing a dense population of pleomorphic synaptic vesicles, and a long-sized synaptic zone (arrow), with a symmetrical contact on a proximal dendrite of a centrifugal neuron. Scale bars: 0.5 μm.</note><note type="content">Fig. 2: Examples of single glutamate (A–C) and double GABA-glutamate (D) immunolabeling in the pigeon ION. (A) The upper left shows a GLU-ir P1a axon terminal contacting a long spine-like profile (arrow). This micrograph also illustrates a P3 axon terminal with rounded-shaped synaptic vesicles, establishing an asymmetrical contact (arrow) upon dendrite of an ION centrifugal neuron. Note the presence of dense-core vesicles (arrowhead). (B) Another large P1a axon terminal is close to a P4 GLU-immunonegative bouton with a clear axoplasm in which the synaptic vesicles are dense. The synaptic zone is long and asymmetrical (arrow). Note the presence of a clear-cut postsynaptic differentiation. (C) The left part of the micrograph is occupied by a P5 axon terminal. The axoplasm is clear and contains a mixture of small rounded synaptic vesicles and numerous large dense-core vesicles. No glutamate immunoreactivity is present. Another P1b axon terminal lies in the upper right part of the micrograph. (D) P2a axon terminal characterized by pleomorphic synaptic vesicles which make a symmetrical synaptic contact (arrow) on a large dendritic profile of an ION centrifugal neuron. Whereas the bouton is GABA-immunoreactive, the postsynaptic dendrite is densely GLU-ir. Scale bars: 0.5 μm.</note><note type="content">Table 1: Glutamate immunogold labeling</note><subject lang="en"><genre>Keywords</genre><topic>Centrifugal visual system</topic><topic>Isthmo-optic nucleus</topic><topic>Afferent synaptic terminals</topic><topic>Glutamate immunoreactivity</topic><topic>Pigeon</topic><topic>Electron microscopy</topic></subject><relatedItem type="host"><titleInfo><title>Brain Research</title></titleInfo><titleInfo type="abbreviated"><title>BRES</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000616</dateIssued></originInfo><identifier type="ISSN">0006-8993</identifier><identifier type="PII">S0006-8993(00)X0272-7</identifier><part><date>20000616</date><detail type="volume"><number>868</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>164</end></extent><extent unit="pages"><start>128</start><end>134</end></extent></part></relatedItem><identifier type="istex">80EC10732A9863B90356AB25C6BE4CE6C3850C17</identifier><identifier type="DOI">10.1016/S0006-8993(00)02316-7</identifier><identifier type="PII">S0006-8993(00)02316-7</identifier><identifier type="ArticleID">28406</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©2000</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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