Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study
Identifieur interne : 000B55 ( Istex/Corpus ); précédent : 000B54; suivant : 000B56Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study
Auteurs : Lysiane Richert ; Eliane Alexandre ; Tom Lloyd ; Samantha Orr ; Catherine Viollon-Abadie ; Rakhee Patel ; Shaun Kingston ; David Berry ; Ashley Dennison ; Bruno Heyd ; Georges Mantion ; Daniel JaeckSource :
- Liver International [ 1478-3223 ] ; 2004-08.
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- KwdEn :
Abstract
Abstract: Background: The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to predict cytochrome P‐450 induction.
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DOI: 10.1111/j.1478-3231.2004.0930.x
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ISTEX:977D9C70C7B25EE1D3BDDB67D18655B933794A84Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title><author><name sortKey="Richert, Lysiane" sort="Richert, Lysiane" uniqKey="Richert L" first="Lysiane" last="Richert">Lysiane Richert</name><affiliation><mods:affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation></author><author><name sortKey="Alexandre, Eliane" sort="Alexandre, Eliane" uniqKey="Alexandre E" first="Eliane" last="Alexandre">Eliane Alexandre</name><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation></author><author><name sortKey="Lloyd, Tom" sort="Lloyd, Tom" uniqKey="Lloyd T" first="Tom" last="Lloyd">Tom Lloyd</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Orr, Samantha" sort="Orr, Samantha" uniqKey="Orr S" first="Samantha" last="Orr">Samantha Orr</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Viollon Badie, Catherine" sort="Viollon Badie, Catherine" uniqKey="Viollon Badie C" first="Catherine" last="Viollon-Abadie">Catherine Viollon-Abadie</name><affiliation><mods:affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Patel, Rakhee" sort="Patel, Rakhee" uniqKey="Patel R" first="Rakhee" last="Patel">Rakhee Patel</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Kingston, Shaun" sort="Kingston, Shaun" uniqKey="Kingston S" first="Shaun" last="Kingston">Shaun Kingston</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Berry, David" sort="Berry, David" uniqKey="Berry D" first="David" last="Berry">David Berry</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Dennison, Ashley" sort="Dennison, Ashley" uniqKey="Dennison A" first="Ashley" last="Dennison">Ashley Dennison</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Heyd, Bruno" sort="Heyd, Bruno" uniqKey="Heyd B" first="Bruno" last="Heyd">Bruno Heyd</name><affiliation><mods:affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Mantion, Georges" sort="Mantion, Georges" uniqKey="Mantion G" first="Georges" last="Mantion">Georges Mantion</name><affiliation><mods:affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Jaeck, Daniel" sort="Jaeck, Daniel" uniqKey="Jaeck D" first="Daniel" last="Jaeck">Daniel Jaeck</name><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:977D9C70C7B25EE1D3BDDB67D18655B933794A84</idno><date when="2004" year="2004">2004</date><idno type="doi">10.1111/j.1478-3231.2004.0930.x</idno><idno type="url">https://api.istex.fr/document/977D9C70C7B25EE1D3BDDB67D18655B933794A84/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B55</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B55</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title><author><name sortKey="Richert, Lysiane" sort="Richert, Lysiane" uniqKey="Richert L" first="Lysiane" last="Richert">Lysiane Richert</name><affiliation><mods:affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation></author><author><name sortKey="Alexandre, Eliane" sort="Alexandre, Eliane" uniqKey="Alexandre E" first="Eliane" last="Alexandre">Eliane Alexandre</name><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation></author><author><name sortKey="Lloyd, Tom" sort="Lloyd, Tom" uniqKey="Lloyd T" first="Tom" last="Lloyd">Tom Lloyd</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Orr, Samantha" sort="Orr, Samantha" uniqKey="Orr S" first="Samantha" last="Orr">Samantha Orr</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Viollon Badie, Catherine" sort="Viollon Badie, Catherine" uniqKey="Viollon Badie C" first="Catherine" last="Viollon-Abadie">Catherine Viollon-Abadie</name><affiliation><mods:affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Patel, Rakhee" sort="Patel, Rakhee" uniqKey="Patel R" first="Rakhee" last="Patel">Rakhee Patel</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Kingston, Shaun" sort="Kingston, Shaun" uniqKey="Kingston S" first="Shaun" last="Kingston">Shaun Kingston</name><affiliation><mods:affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Berry, David" sort="Berry, David" uniqKey="Berry D" first="David" last="Berry">David Berry</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Dennison, Ashley" sort="Dennison, Ashley" uniqKey="Dennison A" first="Ashley" last="Dennison">Ashley Dennison</name><affiliation><mods:affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</mods:affiliation></affiliation></author><author><name sortKey="Heyd, Bruno" sort="Heyd, Bruno" uniqKey="Heyd B" first="Bruno" last="Heyd">Bruno Heyd</name><affiliation><mods:affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Mantion, Georges" sort="Mantion, Georges" uniqKey="Mantion G" first="Georges" last="Mantion">Georges Mantion</name><affiliation><mods:affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</mods:affiliation></affiliation></author><author><name sortKey="Jaeck, Daniel" sort="Jaeck, Daniel" uniqKey="Jaeck D" first="Daniel" last="Jaeck">Daniel Jaeck</name><affiliation><mods:affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</mods:affiliation></affiliation><affiliation><mods:affiliation>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Liver International</title><idno type="ISSN">1478-3223</idno><idno type="eISSN">1478-3231</idno><imprint><publisher>Munksgaard International Publishers</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08">2004-08</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="371">371</biblScope><biblScope unit="page" to="378">378</biblScope></imprint><idno type="ISSN">1478-3223</idno></series><idno type="istex">977D9C70C7B25EE1D3BDDB67D18655B933794A84</idno><idno type="DOI">10.1111/j.1478-3231.2004.0930.x</idno><idno type="ArticleID">LIV930</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1478-3223</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>cell yield</term><term>donor</term><term>hepatocyte</term><term>human</term><term>isolation</term><term>liver resection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Abstract: Background: The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to predict cytochrome P‐450 induction.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Lysiane Richert</name><affiliations><json:string>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</json:string><json:string>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</json:string></affiliations></json:item><json:item><name>Eliane Alexandre</name><affiliations><json:string>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</json:string></affiliations></json:item><json:item><name>Tom Lloyd</name><affiliations><json:string>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</json:string><json:string>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</json:string></affiliations></json:item><json:item><name>Samantha Orr</name><affiliations><json:string>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</json:string></affiliations></json:item><json:item><name>Catherine Viollon‐Abadie</name><affiliations><json:string>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</json:string><json:string>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</json:string></affiliations></json:item><json:item><name>Rakhee Patel</name><affiliations><json:string>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</json:string></affiliations></json:item><json:item><name>Shaun Kingston</name><affiliations><json:string>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</json:string></affiliations></json:item><json:item><name>David Berry</name><affiliations><json:string>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</json:string></affiliations></json:item><json:item><name>Ashley Dennison</name><affiliations><json:string>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</json:string></affiliations></json:item><json:item><name>Bruno Heyd</name><affiliations><json:string>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</json:string></affiliations></json:item><json:item><name>Georges Mantion</name><affiliations><json:string>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</json:string></affiliations></json:item><json:item><name>Daniel Jaeck</name><affiliations><json:string>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</json:string><json:string>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>cell yield</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>donor</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>hepatocyte</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>human</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>isolation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>liver resection</value></json:item></subject><articleId><json:string>LIV930</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><qualityIndicators><score>5.35</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595 x 782 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Davidson</name></json:item></author><host><volume>38</volume><pages><last>8</last><first>165</first></pages><author></author><title>a practical approach using University of Wisconsin solution</title></host><title>Isolation of human hepatocytes after hepatic warm and cold ischaemia</title></json:item></refBibs><genre><json:string>article</json:string></genre><host><volume>24</volume><publisherId><json:string>LIV</json:string></publisherId><pages><total>8</total><last>378</last><first>371</first></pages><issn><json:string>1478-3223</json:string></issn><issue>4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1478-3231</json:string></eissn><title>Liver International</title><doi><json:string>10.1111/(ISSN)1478-3231</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>gastroenterology & hepatology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>gastroenterology & hepatology</json:string></scienceMetrix></categories><publicationDate>2004</publicationDate><copyrightDate>2004</copyrightDate><doi><json:string>10.1111/j.1478-3231.2004.0930.x</json:string></doi><id>977D9C70C7B25EE1D3BDDB67D18655B933794A84</id><score>0.1676233</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/977D9C70C7B25EE1D3BDDB67D18655B933794A84/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/977D9C70C7B25EE1D3BDDB67D18655B933794A84/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/977D9C70C7B25EE1D3BDDB67D18655B933794A84/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Munksgaard International Publishers</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>2004</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title><author xml:id="author-1"><persName><forename type="first">Lysiane</forename><surname>Richert</surname></persName><affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation></author><author xml:id="author-2"><persName><forename type="first">Eliane</forename><surname>Alexandre</surname></persName><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation></author><author xml:id="author-3"><persName><forename type="first">Tom</forename><surname>Lloyd</surname></persName><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation></author><author xml:id="author-4"><persName><forename type="first">Samantha</forename><surname>Orr</surname></persName><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation></author><author xml:id="author-5"><persName><forename type="first">Catherine</forename><surname>Viollon‐Abadie</surname></persName><affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation><affiliation>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation></author><author xml:id="author-6"><persName><forename type="first">Rakhee</forename><surname>Patel</surname></persName><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation></author><author xml:id="author-7"><persName><forename type="first">Shaun</forename><surname>Kingston</surname></persName><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation></author><author xml:id="author-8"><persName><forename type="first">David</forename><surname>Berry</surname></persName><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation></author><author xml:id="author-9"><persName><forename type="first">Ashley</forename><surname>Dennison</surname></persName><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation></author><author xml:id="author-10"><persName><forename type="first">Bruno</forename><surname>Heyd</surname></persName><affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</affiliation></author><author xml:id="author-11"><persName><forename type="first">Georges</forename><surname>Mantion</surname></persName><affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</affiliation></author><author xml:id="author-12"><persName><forename type="first">Daniel</forename><surname>Jaeck</surname></persName><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation><affiliation>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</affiliation></author></analytic><monogr><title level="j">Liver International</title><idno type="pISSN">1478-3223</idno><idno type="eISSN">1478-3231</idno><idno type="DOI">10.1111/(ISSN)1478-3231</idno><imprint><publisher>Munksgaard International Publishers</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08"></date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="371">371</biblScope><biblScope unit="page" to="378">378</biblScope></imprint></monogr><idno type="istex">977D9C70C7B25EE1D3BDDB67D18655B933794A84</idno><idno type="DOI">10.1111/j.1478-3231.2004.0930.x</idno><idno type="ArticleID">LIV930</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2004</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Abstract: Background: The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to predict cytochrome P‐450 induction.</p></abstract><abstract><p>Aims and Methods: As first stage of this prevalidation study, the purpose of the present work was to set criteria for optimization and harmonization of hepatocyte isolation from human tissue among laboratories to establish a routine procedure. This was achieved by combining and/or comparing the data generated by the two independent European laboratories (France and UK).</p></abstract><abstract><p>Results: The results confirmed that surgical waste material is a valuable source for obtaining high quality hepatocytes under certain pre‐, intra‐ and post‐operative conditions: cell yield of viable hepatocytes was not significantly affected by age and sex of patients, nor indications for resection, steatosis or cholestasis. Cold ischeamia up to 5 hours did not influence viable cell yield allowing transport of material.</p></abstract><abstract><p>Conclusion: The use of biopsy sizes between 50–100 g, cannulation with 2–4 cannulae, digestion with collagenase‐containing digestion medium at a flow rate of 25 ml/cannula for 20 minutes, with cut surface being glued in order to reform Glisson's capsule, should optimize the total yield of viable human hepatocytes obtained per preparation of waste liver surgical resecitons.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>cell yield</term></item><item><term>donor</term></item><item><term>hepatocyte</term></item><item><term>human</term></item><item><term>isolation</term></item><item><term>liver resection</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2004-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/977D9C70C7B25EE1D3BDDB67D18655B933794A84/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Munksgaard International Publishers</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1478-3231</doi><issn type="print">1478-3223</issn><issn type="electronic">1478-3231</issn><idGroup><id type="product" value="LIV"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="LIVER INTERNATIONAL">Liver International</title></titleGroup></publicationMeta><publicationMeta level="part" position="08004"><doi origin="wiley">10.1111/liv.2004.24.issue-4</doi><numberingGroup><numbering type="journalVolume" number="24">24</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="2004-08">August 2004</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="13" status="forIssue"><doi origin="wiley">10.1111/j.1478-3231.2004.0930.x</doi><idGroup><id type="unit" value="LIV930"></id><id type="supplier" value="930"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="tocHeading1">Basic Studies</title></titleGroup><eventGroup><event type="firstOnline" date="2004-07-02"></event><event type="publishedOnlineFinalForm" date="2004-07-02"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="371">371</numbering><numbering type="pageLast" number="378">378</numbering></numberingGroup><correspondenceTo>Lysiane Richert, Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, 4, Place Saint‐Jacques, 25030 Besançon Cedex, France.
Tel: +33 3 81 66 55 52
Fax: +33 3 81 66 56 59
e‐mail: <email normalForm="lysiane.richert@univ-fcomte.fr">lysiane.richert@univ‐fcomte.fr</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:LIV.LIV930.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 9 October 2003, accepted 16 February 2004</unparsedEditorialHistory><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="6"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="26"></count><count type="wordTotal" number="6680"></count><count type="linksCrossRef" number="68"></count></countGroup><titleGroup><title type="main">Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title><title type="shortAuthors">Richert et al.</title><title type="short">Human hepatocyte isolation</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>Lysiane</givenNames><familyName>Richert</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Eliane</givenNames><familyName>Alexandre</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3 #a4"><personName><givenNames>Tom</givenNames><familyName>Lloyd</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a4"><personName><givenNames>Samantha</givenNames><familyName>Orr</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1 #a5"><personName><givenNames>Catherine</givenNames><familyName>Viollon‐Abadie</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a4"><personName><givenNames>Rakhee</givenNames><familyName>Patel</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a4"><personName><givenNames>Shaun</givenNames><familyName>Kingston</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a3"><personName><givenNames>David</givenNames><familyName>Berry</familyName></personName></creator><creator creatorRole="author" xml:id="cr9" affiliationRef="#a3"><personName><givenNames>Ashley</givenNames><familyName>Dennison</familyName></personName></creator><creator creatorRole="author" xml:id="cr10" affiliationRef="#a6"><personName><givenNames>Bruno</givenNames><familyName>Heyd</familyName></personName></creator><creator creatorRole="author" xml:id="cr11" affiliationRef="#a6"><personName><givenNames>Georges</givenNames><familyName>Mantion</familyName></personName></creator><creator creatorRole="author" xml:id="cr12" affiliationRef="#a2 #a7"><personName><givenNames>Daniel</givenNames><familyName>Jaeck</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</unparsedAffiliation></affiliation><affiliation xml:id="a3"><unparsedAffiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</unparsedAffiliation></affiliation><affiliation xml:id="a4"><unparsedAffiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</unparsedAffiliation></affiliation><affiliation xml:id="a5" countryCode="FR"><unparsedAffiliation>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</unparsedAffiliation></affiliation><affiliation xml:id="a6" countryCode="FR"><unparsedAffiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</unparsedAffiliation></affiliation><affiliation xml:id="a7" countryCode="FR"><unparsedAffiliation>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">cell yield</keyword><keyword xml:id="k2">donor</keyword><keyword xml:id="k3">hepatocyte</keyword><keyword xml:id="k4">human</keyword><keyword xml:id="k5">isolation</keyword><keyword xml:id="k6">liver resection</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><!-- Richert L, Alexandre E, Lloyd T, Orr S, Viollon-Abadie C, Patel R, Kingston S, Berry D, Dennison A, Heyd B, Mantion G, Jaeck D. Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study.
Liver International 2004: DOI: 10.1111/j.1478-3231.2004.0930.x. © Blackwell Munksgaard 2004
<p><b>Abstract: Background: </b> The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to predict cytochrome P‐450 induction.</p><p><b>Aims and Methods: </b> As first stage of this prevalidation study, the purpose of the present work was to set criteria for optimization and harmonization of hepatocyte isolation from human tissue among laboratories to establish a routine procedure. This was achieved by combining and/or comparing the data generated by the two independent European laboratories (France and UK).</p><p><b>Results: </b> The results confirmed that surgical waste material is a valuable source for obtaining high quality hepatocytes under certain pre‐, intra‐ and post‐operative conditions: cell yield of viable hepatocytes was not significantly affected by age and sex of patients, nor indications for resection, steatosis or cholestasis. Cold ischeamia up to 5 hours did not influence viable cell yield allowing transport of material.</p><p><b>Conclusion: </b> The use of biopsy sizes between 50–100 g, cannulation with 2–4 cannulae, digestion with collagenase‐containing digestion medium at a flow rate of 25 ml/cannula for 20 minutes, with cut surface being glued in order to reform Glisson's capsule, should optimize the total yield of viable human hepatocytes obtained per preparation of waste liver surgical resecitons.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Human hepatocyte isolation</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study</title></titleInfo><name type="personal"><namePart type="given">Lysiane</namePart><namePart type="family">Richert</namePart><affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eliane</namePart><namePart type="family">Alexandre</namePart><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tom</namePart><namePart type="family">Lloyd</namePart><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Samantha</namePart><namePart type="family">Orr</namePart><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Catherine</namePart><namePart type="family">Viollon‐Abadie</namePart><affiliation>Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation><affiliation>KaLy‐Cell, Faculté de Médecine et de Pharmacie, Besançon, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rakhee</namePart><namePart type="family">Patel</namePart><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shaun</namePart><namePart type="family">Kingston</namePart><affiliation>UK Human Tissue Bank, De Montfort University, Oxford Road Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David</namePart><namePart type="family">Berry</namePart><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ashley</namePart><namePart type="family">Dennison</namePart><affiliation>Department of Surgery, Leicester General Hospital, Gwendolen Road, Leicester, UK,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bruno</namePart><namePart type="family">Heyd</namePart><affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Georges</namePart><namePart type="family">Mantion</namePart><affiliation>Service de Chirurgie Viscérale et Digestive – Centre de Transplantation Hépatique, Hôpital Jean Minjoz, Besançon, France,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Jaeck</namePart><affiliation>Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France,</affiliation><affiliation>Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Strasbourg, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Munksgaard International Publishers</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2004-08</dateIssued><edition>Received 9 October 2003, accepted 16 February 2004</edition><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">6</extent><extent unit="references">26</extent><extent unit="words">6680</extent></physicalDescription><abstract>Abstract: Background: The European Center for Validation of Alternative Methods (ECVAM) has funded a prevalidation study in three laboratories (France, USA and UK) on the use of human hepatocyte cultures to predict cytochrome P‐450 induction.</abstract><abstract>Aims and Methods: As first stage of this prevalidation study, the purpose of the present work was to set criteria for optimization and harmonization of hepatocyte isolation from human tissue among laboratories to establish a routine procedure. This was achieved by combining and/or comparing the data generated by the two independent European laboratories (France and UK).</abstract><abstract>Results: The results confirmed that surgical waste material is a valuable source for obtaining high quality hepatocytes under certain pre‐, intra‐ and post‐operative conditions: cell yield of viable hepatocytes was not significantly affected by age and sex of patients, nor indications for resection, steatosis or cholestasis. Cold ischeamia up to 5 hours did not influence viable cell yield allowing transport of material.</abstract><abstract>Conclusion: The use of biopsy sizes between 50–100 g, cannulation with 2–4 cannulae, digestion with collagenase‐containing digestion medium at a flow rate of 25 ml/cannula for 20 minutes, with cut surface being glued in order to reform Glisson's capsule, should optimize the total yield of viable human hepatocytes obtained per preparation of waste liver surgical resecitons.</abstract><subject lang="en"><genre>keywords</genre><topic>cell yield</topic><topic>donor</topic><topic>hepatocyte</topic><topic>human</topic><topic>isolation</topic><topic>liver resection</topic></subject><relatedItem type="host"><titleInfo><title>Liver International</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1478-3223</identifier><identifier type="eISSN">1478-3231</identifier><identifier type="DOI">10.1111/(ISSN)1478-3231</identifier><identifier type="PublisherID">LIV</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>371</start><end>378</end><total>8</total></extent></part></relatedItem><identifier type="istex">977D9C70C7B25EE1D3BDDB67D18655B933794A84</identifier><identifier type="DOI">10.1111/j.1478-3231.2004.0930.x</identifier><identifier type="ArticleID">LIV930</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Munksgaard International Publishers</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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